FastQCFastQC Report
Thu 26 May 2016
SRR1513193_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513193_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271976
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC24770.10902403898632733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGT500.001514889613.2824816
CTTAGAC2450.011.6692153
TAGGACC3750.010.9280544
TTAGGAC4900.010.696783
GTCCTAA3850.010.6699241
AGGACGT5450.010.4700595
GTCCTAC7250.010.4098061
TGGACTG3750.010.3979325
GGACGTG5550.010.0857966
GTATTAG2002.1827873E-1110.0309691
TGGCGAG6350.09.86274718
GTCTAGG1752.3374014E-99.8262551
GTCTTAG3500.09.8262551
GACGTGA3300.09.7749877
GTCTAGC1606.088885E-89.5533031
TAGGACG5800.09.5302794
CTTATAC1803.963578E-99.5298593
AGGACCG2002.6011548E-109.51030355
AGAACCG1002.7262475E-49.51030355
TGCGGAA800.0045595489.48915818
Produced by FastQC (version 0.11.5)