##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513193_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2271976
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.153981380085	31.0	30.0	34.0	23.0	34.0
2	29.019143247992055	31.0	30.0	34.0	16.0	34.0
3	29.197294337616242	31.0	30.0	34.0	22.0	34.0
4	32.25301543678278	35.0	33.0	37.0	19.0	37.0
5	32.38885269914823	35.0	33.0	37.0	22.0	37.0
6	32.75694153459367	37.0	33.0	37.0	25.0	37.0
7	32.71531477445184	37.0	35.0	37.0	25.0	37.0
8	32.590879921266776	37.0	35.0	37.0	23.0	37.0
9	33.80198822522773	39.0	34.0	39.0	21.0	39.0
10	33.377561206632464	38.0	33.0	39.0	17.0	39.0
11	33.82260728106283	39.0	34.0	39.0	23.0	39.0
12	33.80485005123293	39.0	34.0	39.0	23.0	39.0
13	33.84384254059022	39.0	34.0	39.0	23.0	39.0
14	34.04850843494825	39.0	33.0	41.0	11.0	41.0
15	34.460338049345594	39.0	34.0	41.0	17.0	41.0
16	34.299941988823825	39.0	34.0	41.0	17.0	41.0
17	33.90229782356856	39.0	33.0	41.0	11.0	41.0
18	33.62740231410895	39.0	32.0	41.0	10.0	41.0
19	34.26031172864502	39.0	34.0	41.0	18.0	41.0
20	34.468802047204726	39.0	34.0	41.0	18.0	41.0
21	34.513368539104285	39.0	34.0	41.0	18.0	41.0
22	34.19091971041948	39.0	34.0	41.0	10.0	41.0
23	33.72374091979845	39.0	33.0	41.0	8.0	41.0
24	33.96193841836357	39.0	34.0	41.0	2.0	41.0
25	33.60552708303257	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.1661673187108974
1101	2	1.428704747270455
1101	3	1.268851689368237
1101	4	1.6570621477806924
1101	5	1.5741252050504286
1101	6	1.2838466121119652
1101	7	1.1561565104030223
1101	8	1.2576877619724058
1101	9	1.5646453922262609
1101	10	2.0939707753681773
1101	11	1.6716980889127484
1101	12	1.4308805218608
1101	13	1.4055263957242516
1101	14	2.3930367835116684
1101	15	2.0058097188263986
1101	16	1.9959824245716788
1101	17	2.473438505084438
1101	18	2.4022726397694996
1101	19	2.050495886794124
1101	20	1.6869387778446878
1101	21	1.690591425791638
1101	22	1.8851628681860149
1101	23	2.1799839502085163
1101	24	1.842026919527214
1101	25	1.953157950667574
1102	1	0.9714009215860102
1102	2	1.1830585957280775
1102	3	1.1073549837677596
1102	4	1.472135897406325
1102	5	1.40584503134572
1102	6	1.0107502509259803
1102	7	1.0268138928528572
1102	8	1.022222428040095
1102	9	1.4083186476919707
1102	10	1.596505216817711
1102	11	1.306324848421582
1102	12	1.1706660115283682
1102	13	1.11139080123489
1102	14	2.1659852710827963
1102	15	1.6876081614738965
1102	16	1.7601408552395412
1102	17	2.113547819250357
1102	18	2.2851548649866302
1102	19	1.739502700211311
1102	20	1.5582063729330358
1102	21	1.357359170625422
1102	22	1.614863002956902
1102	23	1.8949042107655671
1102	24	1.684970166008199
1102	25	2.0628071719913237
1103	1	0.9553741402835065
1103	2	1.2532552201827798
1103	3	0.9447459244310075
1103	4	1.4350191050920102
1103	5	1.2323872150736435
1103	6	0.9697059107173231
1103	7	0.9839895736795867
1103	8	0.9324500938173443
1103	9	1.3926949271097513
1103	10	1.7315060285862387
1103	11	1.2365161963808688
1103	12	1.2443303875960154
1103	13	1.193788218399206
1103	14	2.0557594582554444
1103	15	1.677596232005996
1103	16	1.720552315999356
1103	17	2.0432730179571763
1103	18	2.3865379597306102
1103	19	1.6375547204561158
1103	20	1.3675341227106799
1103	21	1.4601839486243762
1103	22	1.7173024556131509
1103	23	2.1622590277294123
1103	24	1.7698972127237127
1103	25	1.8387243821899588
1104	1	0.8031027582409536
1104	2	0.9955125397910471
1104	3	0.833345186413883
1104	4	1.236268440810555
1104	5	1.096556386227654
1104	6	0.8581212245205805
1104	7	0.7522577046096828
1104	8	0.7589726234289174
1104	9	1.0725052719313481
1104	10	1.4017311700360437
1104	11	0.9432362395900071
1104	12	0.8839953673034984
1104	13	0.8727348669971846
1104	14	1.9047992609225801
1104	15	1.4199994415498196
1104	16	1.4664337709990463
1104	17	1.9590496894005298
1104	18	1.9120190837104687
1104	19	1.1836755774513037
1104	20	1.001773195910765
1104	21	0.989924207191045
1104	22	1.203700369673811
1104	23	1.4564960016436999
1104	24	1.3229642206054208
1104	25	1.6805313806665865
1105	1	0.596249539615112
1105	2	0.781899686515402
1105	3	0.6584050653884539
1105	4	1.0266146585855722
1105	5	0.9809564749855895
1105	6	0.6613293567259362
1105	7	0.6457446617616469
1105	8	0.6943919514310508
1105	9	0.8618551956249405
1105	10	1.2289053186691774
1105	11	0.7733486056244345
1105	12	0.7237978568536647
1105	13	0.6477811609240334
1105	14	1.4421474939561278
1105	15	1.0282779394935133
1105	16	1.285412924219493
1105	17	1.6145066880014909
1105	18	1.1954560767400153
1105	19	1.0516770022497397
1105	20	0.9213363172635383
1105	21	0.803411205561865
1105	22	0.8534304376952306
1105	23	1.272707480476619
1105	24	1.0644315740698147
1105	25	1.2907353143880442
1106	1	0.7012681782231773
1106	2	0.9929564044090711
1106	3	0.8981103567133957
1106	4	1.3356973175496023
1106	5	1.1502245088004202
1106	6	0.9369749664759155
1106	7	0.9179327297681539
1106	8	0.955463578482572
1106	9	1.2907564900855633
1106	10	1.1987228737920077
1106	11	1.0432901876546197
1106	12	0.9723700781107496
1106	13	0.9426091956723113
1106	14	2.004997070492827
1106	15	1.6122710354730572
1106	16	1.5850565737393296
1106	17	1.9517800147834379
1106	18	2.0954556445905084
1106	19	1.533895346650418
1106	20	1.3279335115761057
1106	21	1.2039405022731202
1106	22	1.5518915533151016
1106	23	1.8355357952577762
1106	24	1.4849648984144395
1106	25	1.8404804758261335
1107	1	0.7024404105523274
1107	2	0.920035947644454
1107	3	0.9235715923624142
1107	4	1.1269340971740363
1107	5	1.0479617560843977
1107	6	0.761483410043283
1107	7	0.7183063434916477
1107	8	0.8544306161934045
1107	9	0.991681017029947
1107	10	1.2837438927759024
1107	11	0.8876474384331985
1107	12	0.7849444668129166
1107	13	0.7735641461826219
1107	14	1.6844231340328548
1107	15	1.1231772671649551
1107	16	1.2546530503614974
1107	17	1.7762365532805546
1107	18	1.9598862819436604
1107	19	1.2988450347060834
1107	20	1.0795913903546435
1107	21	0.8891100417055569
1107	22	1.2033865221206668
1107	23	1.5739039204055203
1107	24	1.315443452883926
1107	25	1.5925618177520633
1108	1	0.562074962047344
1108	2	0.8106242306036933
1108	3	0.4776866019510493
1108	4	0.9584712497548651
1108	5	0.8716997027661932
1108	6	0.5214299297742642
1108	7	0.3128624302485292
1108	8	0.5235282041792502
1108	9	0.6643507088431448
1108	10	0.9410312546860666
1108	11	0.6019939257518914
1108	12	0.3737946410289439
1108	13	0.4290485747810635
1108	14	1.237052580593101
1108	15	0.8256189925788888
1108	16	0.8544221128361755
1108	17	1.2737178486915184
1108	18	1.461432618005027
1108	19	0.8880801047976519
1108	20	0.715743241906381
1108	21	0.7372719950858908
1108	22	0.9746233208673942
1108	23	1.3096425153330031
1108	24	0.9754318384820664
1108	25	1.3502327250259256
1109	1	0.3369451976321436
1109	2	0.6182872611147339
1109	3	0.5505302564504646
1109	4	0.7619731899155511
1109	5	0.8789091883782802
1109	6	0.47685019032982723
1109	7	0.39710577569120886
1109	8	0.34113566319403077
1109	9	0.5984874318198621
1109	10	0.9977724594883028
1109	11	0.6554890129435549
1109	12	0.5622628053788787
1109	13	0.42744035731696783
1109	14	1.3873297568324858
1109	15	0.8951591916085704
1109	16	1.045486457592581
1109	17	1.4098225172908556
1109	18	1.519536630990146
1109	19	0.8212372992553938
1109	20	0.7289277055038141
1109	21	0.6229369788147494
1109	22	1.0258168647704338
1109	23	1.3158128637606552
1109	24	1.1088911799385102
1109	25	1.3440178525746802
1110	1	0.6053082404877053
1110	2	0.844318586089134
1110	3	0.7441571645782332
1110	4	1.0696921078297663
1110	5	0.9192547064330796
1110	6	0.5282523761786706
1110	7	0.547392028520953
1110	8	0.5467134551228412
1110	9	0.736108585708223
1110	10	1.0449919364985547
1110	11	0.6747960554312371
1110	12	0.6134457583722792
1110	13	0.42229493727783307
1110	14	1.265940757916276
1110	15	0.8801918663080954
1110	16	1.0322190511510243
1110	17	1.3104235304569656
1110	18	0.9580455935299454
1110	19	0.7447903121636017
1110	20	0.5165315064725391
1110	21	0.4294837204734705
1110	22	0.7172411941827903
1110	23	-0.6262091706285986
1110	24	0.17004637388803445
1110	25	0.6436389825057987
1111	1	0.41345475639426965
1111	2	0.7409836905447627
1111	3	0.624437790341716
1111	4	1.0369141317088975
1111	5	0.8767916179449671
1111	6	0.6123436980393393
1111	7	0.581926092600014
1111	8	0.6764444044412699
1111	9	0.8223567850708164
1111	10	1.1682287734970203
1111	11	0.8216640814599288
1111	12	0.572370078110751
1111	13	0.5111563933124259
1111	14	1.260104002646223
1111	15	0.9114598260335285
1111	16	0.9495292876036387
1111	17	1.3581775074088114
1111	18	1.6411599218766497
1111	19	1.1977043437005648
1111	20	0.7599762844374638
1111	21	0.4686160184973076
1111	22	0.8433185444655393
1111	23	1.3887068867032042
1111	24	0.9117716830752016
1111	25	1.1884708888054831
1112	1	0.2665823274817001
1112	2	0.4835971444809104
1112	3	0.4276781130194607
1112	4	0.8837713849346684
1112	5	0.5600116980123886
1112	6	0.36620660013232964
1112	7	0.14019257766010895
1112	8	0.34570598813079556
1112	9	0.5010624118643037
1112	10	0.7696061238471898
1112	11	0.317421787197091
1112	12	0.32093528590478115
1112	13	0.2616317309401026
1112	14	0.7867335704025251
1112	15	0.6068825378911313
1112	16	0.46059436997877157
1112	17	0.9938199280938989
1112	18	1.1138254999072714
1112	19	0.568230024474353
1112	20	0.4298900464024271
1112	21	0.41396347901544317
1112	22	0.6077059734784456
1112	23	0.9561145282258892
1112	24	0.692821639931104
1112	25	1.1038927728983552
1113	1	0.024166305486360784
1113	2	0.13618284877564335
1113	3	0.12857938819768577
1113	4	0.34992866956259405
1113	5	0.4664965730555437
1113	6	0.014224907659837527
1113	7	0.05369422111355249
1113	8	0.10029804618472582
1113	9	0.3142577494899683
1113	10	0.4835198637250002
1113	11	0.1457431882617044
1113	12	0.018347305183581852
1113	13	-0.1240464481210708
1113	14	0.5165383478628698
1113	15	0.330620006479613
1113	16	0.37401823552797
1113	17	0.726479723746678
1113	18	0.6396988256272209
1113	19	0.4257453302806269
1113	20	0.26155969139372104
1113	21	0.16198662394918983
1113	22	0.3807772395383182
1113	23	0.4280468222440703
1113	24	0.41785698777538727
1113	25	0.8040474118128529
1114	1	-1.1507145516422668
1114	2	-0.8616721400829732
1114	3	-0.7855322748127556
1114	4	-0.5739094802415536
1114	5	-3.5722884849858865
1114	6	-1.572728148036589
1114	7	-1.1889776076013483
1114	8	-0.9125330092467934
1114	9	-0.5687680592606483
1114	10	-0.4152993239092808
1114	11	-0.8567171523156745
1114	12	-0.9436494070637309
1114	13	-0.9014522382479768
1114	14	-6.52601316224132
1114	15	-2.6653820171798124
1114	16	-1.4644345624309807
1114	17	-0.610117873180883
1114	18	-6.667482235609757
1114	19	-2.7225403800841192
1114	20	-1.6147235413605756
1114	21	-1.3439489708230354
1114	22	-1.1904417253762318
1114	23	-0.6763200509877905
1114	24	-0.7257623866202607
1114	25	-0.671325050845283
1115	1	-0.9253076121749046
1115	2	-0.6792657915361815
1115	3	-0.6964006472927906
1115	4	-3.3863821156246487
1115	5	-1.4483458138860108
1115	6	-0.9822616665614845
1115	7	-0.8147051573113053
1115	8	-0.6596711671852766
1115	9	-0.5130303889830969
1115	10	-0.7898851143013701
1115	11	-0.8861980894013257
1115	12	-0.9724594815871086
1115	13	-0.9883313043363628
1115	14	-6.370203041762384
1115	15	-2.8806447463166904
1115	16	-1.6388493715299433
1115	17	-0.7118046787771775
1115	18	-0.42329602071237815
1115	19	-0.7721435737445574
1115	20	-0.7462898857521552
1115	21	-0.9610989368292167
1115	22	-2.173242892795301
1115	23	-1.2728395155330716
1115	24	-0.9741321093220279
1115	25	-0.5769275955126858
1116	1	-0.34339499678133834
1116	2	-0.11204587463149451
1116	3	-0.2183374360573147
1116	4	-0.03973849520786388
1116	5	-0.49015837001482154
1116	6	-0.30078400563378693
1116	7	-0.1710724045232368
1116	8	-0.22774720266933457
1116	9	-0.26663276144311254
1116	10	-0.09332714469832126
1116	11	-0.3609066870497486
1116	12	-0.6786597902209053
1116	13	-0.5905710549361558
1116	14	0.47781714669018527
1116	15	-0.13165811646585013
1116	16	-0.021467502389221238
1116	17	0.36177425268087404
1116	18	0.14350641989430812
1116	19	-0.08499316956517333
1116	20	-0.5375540741029212
1116	21	-0.6518366505601989
1116	22	-0.5831751408720578
1116	23	-1.25104452587194
1116	24	-0.9449739544971436
1116	25	-0.3197790767939068
1201	1	0.9837776474242723
1201	2	1.1061460851617184
1201	3	1.1053156769207604
1201	4	1.4879097069301324
1201	5	1.4771362401369288
1201	6	1.1515592816388605
1201	7	1.0960235293701857
1201	8	1.091884656836939
1201	9	1.3666942464521838
1201	10	1.7870412833153182
1201	11	1.1421853020705015
1201	12	1.1849217878033684
1201	13	1.114611778975096
1201	14	2.04295765767057
1201	15	1.658536592017711
1201	16	1.7260115230332858
1201	17	2.0468036659677438
1201	18	1.3576242225521469
1201	19	1.4985739174556088
1201	20	1.4248483847673583
1201	21	1.330221244449028
1201	22	1.5708840652279648
1201	23	1.7445572820532576
1201	24	1.5087339022719348
1201	25	1.8101074678493134
1202	1	0.8357684031537218
1202	2	0.9546347640300894
1202	3	0.893678366438639
1202	4	1.2886081935424727
1202	5	1.1399149631601233
1202	6	0.7950783040331402
1202	7	0.8099712186469645
1202	8	0.8655116209000084
1202	9	1.2012104377024997
1202	10	1.3881947506056136
1202	11	0.9755890121778918
1202	12	1.0064929004911676
1202	13	1.0582971569087078
1202	14	1.9357272190843489
1202	15	1.3493674053344265
1202	16	1.4440881361309437
1202	17	1.8969989529052214
1202	18	1.9733959675976465
1202	19	1.5113044214818956
1202	20	1.2818239515127843
1202	21	1.2033546526366194
1202	22	1.4128649016209067
1202	23	1.6586248299794946
1202	24	1.285733096325842
1202	25	1.7431113047037812
1203	1	0.48394622474988225
1203	2	0.5625257675512572
1203	3	0.5164588207183485
1203	4	0.8269047113380097
1203	5	0.5584671924640645
1203	6	0.3570503467797792
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2211	20	-1.0764792893464374
2211	21	-1.1040133607450144
2211	22	-0.8226040764259537
2211	23	-0.31442682562546764
2211	24	-0.7857268176298362
2211	25	-8.054966210814616
2212	1	-1.7571486723550436
2212	2	-4.075284351360423
2212	3	-1.9509478788500516
2212	4	-4.045733873688533
2212	5	-1.6170329358827793
2212	6	-1.3468624581324562
2212	7	-1.1684903580563777
2212	8	-1.1968811626159876
2212	9	-1.3070838916429537
2212	10	-0.606745698933203
2212	11	-1.2185993006796139
2212	12	-1.3450598921646204
2212	13	-1.5504369691560527
2212	14	-6.856313752550744
2212	15	-5.869861563344578
2212	16	-3.3391014504974237
2212	17	-7.947872995996079
2212	18	-3.5488951819415284
2212	19	-2.338958984900728
2212	20	-2.4014534956681146
2212	21	-1.9895717602467897
2212	22	-1.8616097754509298
2212	23	-1.3105786441612395
2212	24	-1.7297686983303109
2212	25	-1.4574723927499846
2213	1	-0.25379679567830493
2213	2	-0.30996561179801674
2213	3	-0.20088992244492943
2213	4	0.21689326044860024
2213	5	-0.03477864465473601
2213	6	-0.2390050900079146
2213	7	-0.22593521164200325
2213	8	-0.33132356369173266
2213	9	-0.07706792807611151
2213	10	-3.917984473639219
2213	11	-1.56780957685028
2213	12	-0.8436855817646531
2213	13	-0.6788420140535365
2213	14	0.15121264593675932
2213	15	-0.472103415257088
2213	16	-0.3175437343687477
2213	17	0.07908114231266694
2213	18	0.2053665604763708
2213	19	-0.24361149113791924
2213	20	-0.5395389762176777
2213	21	-0.546057390121284
2213	22	-0.8297236042661993
2213	23	-1.157754781518321
2213	24	-0.6458848006751552
2213	25	-0.25531934458996375
2214	1	-1.0485287568158554
2214	2	-0.7620113739421335
2214	3	-0.7433346349983196
2214	4	-0.3459521581598928
2214	5	-3.6829737385701975
2214	6	-1.3609191511804681
2214	7	-1.2747419485108331
2214	8	-0.9201121818318043
2214	9	-0.682171299966754
2214	10	-4.277140340572135
2214	11	-1.8636401027564133
2214	12	-1.2181325225318602
2214	13	-0.9203322218899785
2214	14	-0.19367387072495745
2214	15	-0.4999563053233018
2214	16	-0.5547063440540398
2214	17	-0.2677459317894275
2214	18	0.09865474021219
2214	19	-0.6061501100931395
2214	20	-0.8974742236807742
2214	21	-0.9742741908621468
2214	22	-0.7239064170658764
2214	23	-0.4068468706129664
2214	24	-1.0207401845120145
2214	25	-0.7189735993067146
2215	1	-2.3036649545668233
2215	2	-4.8166708415013595
2215	3	-3.1223998628349143
2215	4	-2.0066884485375844
2215	5	-1.8182726987502669
2215	6	-2.094236338571477
2215	7	-2.334333293294332
2215	8	-2.413754185925651
2215	9	-8.036152460909573
2215	10	-3.86080468342697
2215	11	-3.0724547958471895
2215	12	-3.393691460709544
2215	13	-2.7061216681797546
2215	14	-8.104337522066082
2215	15	-4.684116852642418
2215	16	-9.144014047828403
2215	17	-4.389402491308648
2215	18	-2.855863627390555
2215	19	-3.4102020334608127
2215	20	-3.1698733372118895
2215	21	-3.282244160825737
2215	22	-9.187472071389042
2215	23	-10.363924370073882
2215	24	-6.574913609636447
2215	25	-4.9290159505383215
2216	1	-2.1722265293943828
2216	2	-2.153640236347126
2216	3	-1.936440415747981
2216	4	-6.825549914206455
2216	5	-3.7079047906169613
2216	6	-2.875026051120262
2216	7	-2.413597017273908
2216	8	-8.19299649851358
2216	9	-4.1693257999175835
2216	10	-2.550107919962425
2216	11	-2.465013220704371
2216	12	-2.6829193632995327
2216	13	-2.5121602717366756
2216	14	-1.8253324424432904
2216	15	-2.4699038263720894
2216	16	-2.1074670353743272
2216	17	-1.9143696189113442
2216	18	-7.636765979622396
2216	19	-5.143014925483033
2216	20	-4.449532909220501
2216	21	-3.625311270432359
2216	22	-3.30446680523756
2216	23	-2.4767377087140865
2216	24	-2.914860698541009
2216	25	-2.3656761281083796
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117617.0
3	4.0
4	1042.0
5	5827.0
6	6830.0
7	6180.0
8	5616.0
9	5284.0
10	5065.0
11	4764.0
12	4584.0
13	4674.0
14	4591.0
15	4732.0
16	4814.0
17	5283.0
18	5798.0
19	7054.0
20	8931.0
21	11343.0
22	14122.0
23	16853.0
24	19502.0
25	22245.0
26	24860.0
27	28035.0
28	31376.0
29	37580.0
30	44800.0
31	53572.0
32	67975.0
33	87519.0
34	115271.0
35	152383.0
36	214080.0
37	307608.0
38	814162.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39979063566417	17.805140361854594	12.016886724105163	25.77818227837608
2	15.109191194803692	19.77656481195867	40.146756213728516	24.967487779509113
3	18.732734471394604	24.18241930102962	29.2889117575933	27.79593446998247
4	11.760871622476172	16.16944067825092	38.21480657979805	33.85488111947486
5	13.258969291074859	37.3944961253651	35.76044646667099	13.586088116889048
6	32.864319749812466	36.3118821470204	17.685078553392007	13.138719549775136
7	28.717771725182022	31.105768743982683	21.924405554046565	18.252053976788734
8	25.95584778279236	35.36253805694323	20.423041773273066	18.258572386991347
9	26.848039021469734	14.68860743890789	19.3910819389233	39.072271600699075
10	14.235866840531067	28.258614784402567	35.07494160251675	22.430576772549617
11	35.23531799383849	22.090475020712987	23.605142279053428	19.069064706395096
12	23.253915701902994	24.369851120558184	31.01016656041645	21.366066617122367
13	29.173417347017843	20.957824684244265	25.393946224667342	24.474811744070553
14	21.204052603008943	20.515675374400875	28.134755697844838	30.145516324745348
15	24.10394466452937	28.73633972717101	23.08773793059113	24.071977677708485
16	23.446818533005505	27.135414029843517	26.19172666700418	23.226040770146795
17	22.50907934875267	27.308761263324012	27.239323179022566	22.942836208900754
18	22.518219717932833	26.755611206430846	28.176513657578933	22.549655418057387
19	23.949012674188648	26.639129906471286	26.84825313860952	22.563604280730548
20	24.276990675108088	26.396664524025464	26.656951403136475	22.669393397729976
21	24.11072414506677	26.0731409852811	26.44087124597685	23.375263623675277
22	23.927011413273213	26.47619592785684	26.35664717486262	23.24014548400733
23	23.313340721046043	26.2410410775318	26.85358724523288	23.592030956189276
24	23.655699488938147	26.425587791583993	26.576669405598867	23.34204331387899
25	23.616363541098405	26.06857871996428	26.725420498940856	23.58963723999646
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	222.0
1	222.0
2	338.5
3	455.0
4	455.0
5	455.0
6	854.0
7	1253.0
8	1253.0
9	1253.0
10	2076.0
11	2899.0
12	2899.0
13	2899.0
14	5287.5
15	7676.0
16	7676.0
17	7676.0
18	14654.0
19	21632.0
20	21632.0
21	21632.0
22	33804.0
23	45976.0
24	45976.0
25	45976.0
26	65430.5
27	84885.0
28	84885.0
29	84885.0
30	110048.0
31	135211.0
32	135211.0
33	135211.0
34	160847.5
35	186484.0
36	186484.0
37	186484.0
38	210704.5
39	234925.0
40	234925.0
41	234925.0
42	255122.5
43	275320.0
44	275320.0
45	275320.0
46	290982.0
47	306644.0
48	306644.0
49	306644.0
50	302408.5
51	298173.0
52	298173.0
53	298173.0
54	273396.0
55	248619.0
56	248619.0
57	248619.0
58	216287.0
59	183955.0
60	183955.0
61	183955.0
62	151367.5
63	118780.0
64	118780.0
65	118780.0
66	92711.5
67	66643.0
68	66643.0
69	66643.0
70	49102.5
71	31562.0
72	31562.0
73	31562.0
74	22237.5
75	12913.0
76	12913.0
77	12913.0
78	8980.0
79	5047.0
80	5047.0
81	5047.0
82	3473.5
83	1900.0
84	1900.0
85	1900.0
86	1243.0
87	586.0
88	586.0
89	586.0
90	381.0
91	176.0
92	176.0
93	176.0
94	103.0
95	30.0
96	30.0
97	30.0
98	20.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18305651116032917
2	0.25990591449909684
3	0.2596858417518495
4	0.46787466064782374
5	0.25647277964203846
6	0.017605819779786407
7	0.017913921625932666
8	0.016725528790797087
9	0.017869907076483203
10	0.02086289643904689
11	0.01998260545005757
12	0.02676084606527534
13	0.026408729669679606
14	0.024075958548857912
15	0.03886484716387849
16	0.02029070729620383
17	0.03908491991112582
18	0.02962179177949063
19	0.025880555076286015
20	0.027993253449860384
21	0.03283485388930165
22	0.04119761828470019
23	0.028037267999309855
24	0.0448068113395564
25	0.03543171230682014
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2271976.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.83014231773107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31380469821686	56.08324412154941
2	12.672499006304164	17.698448182630504
3	3.6582050253569864	7.663589326443521
4	1.3956965454881085	3.8984675361520096
5	0.6517210350917896	2.2754886315959335
6	0.3561585398198644	1.4922360913977852
7	0.23684927545858434	1.1577453029187104
8	0.14577980965256235	0.814385988407214
9	0.10341996306962853	0.6499647665681987
>10	0.43574436646457115	5.127881845280054
>50	0.01950202969004468	0.9276478643334424
>100	0.009022407383746387	1.1610455116198426
>500	0.0011500545622841656	0.5450714577192969
>1k	4.472434408879571E-4	0.5047833733840132
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2477	0.10902403898632733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.401454944946602E-5	0.0	0.0	0.0
6	8.802909889893203E-5	4.401454944946602E-5	0.0	0.0	0.0
7	8.802909889893203E-5	4.401454944946602E-5	0.0	0.0	0.0
8	8.802909889893203E-5	4.401454944946602E-5	0.0	0.0	0.0
9	8.802909889893203E-5	4.401454944946602E-5	0.0	4.401454944946602E-5	0.0
10	8.802909889893203E-5	4.401454944946602E-5	0.0	4.401454944946602E-5	0.0
11	8.802909889893203E-5	4.401454944946602E-5	0.0	4.401454944946602E-5	0.0
12	8.802909889893203E-5	8.802909889893203E-5	0.0	4.401454944946602E-5	0.0
13	8.802909889893203E-5	8.802909889893203E-5	0.0	4.401454944946602E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACGGT	50	0.0015148896	13.282481	6
CTTAGAC	245	0.0	11.669215	3
TAGGACC	375	0.0	10.928054	4
TTAGGAC	490	0.0	10.69678	3
GTCCTAA	385	0.0	10.669924	1
AGGACGT	545	0.0	10.470059	5
GTCCTAC	725	0.0	10.409806	1
TGGACTG	375	0.0	10.397932	5
GGACGTG	555	0.0	10.085796	6
GTATTAG	200	2.1827873E-11	10.030969	1
TGGCGAG	635	0.0	9.862747	18
GTCTAGG	175	2.3374014E-9	9.826255	1
GTCTTAG	350	0.0	9.826255	1
GACGTGA	330	0.0	9.774987	7
GTCTAGC	160	6.088885E-8	9.553303	1
TAGGACG	580	0.0	9.530279	4
CTTATAC	180	3.963578E-9	9.529859	3
AGGACCG	200	2.6011548E-10	9.5103035	5
AGAACCG	100	2.7262475E-4	9.5103035	5
TGCGGAA	80	0.004559548	9.489158	18
>>END_MODULE