FastQCFastQC Report
Thu 26 May 2016
SRR1513193_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513193_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271976
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31340.1379415979746265No Hit
GTACATGGGAAGCAGTGGTATCAAC27960.12306468026070698No Hit
CCCATGTACTCTGCGTTGATACCAC25820.11364556667852126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGCC551.9239473E-413.8491044
ATTTGCG551.9775859E-413.80428416
CGTGCGC1101.9099389E-1013.80033410
TTGCGCG655.5041117E-513.14119518
CGACGGG550.00309139212.0792814
GTCCTAC7350.010.886971
CAGTACG700.00146429610.8814394
CTAGACA2550.010.8280974
TAGGACA4150.010.7831134
AAGGCGT1701.3460522E-1010.6395276
GTCCTAA4050.010.5845541
TATGGCG6600.010.20941816
TAGGACG5950.010.0813334
TTAGGAC5100.010.0813323
AAAACGC856.654038E-410.04914918
GTAGGAC5900.010.0053913
TAGACTG2201.8189894E-129.9540435
GTACTAG1159.938638E-69.9402761
GTTCTAG2500.09.9071421
GACGTGA3650.09.8800287
Produced by FastQC (version 0.11.5)