##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513193_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2271976 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.68864635894041 31.0 31.0 34.0 27.0 34.0 2 30.77110101515157 31.0 31.0 34.0 27.0 34.0 3 30.876433553875568 33.0 31.0 34.0 27.0 34.0 4 34.2689478233925 37.0 35.0 37.0 30.0 37.0 5 34.01379680067043 37.0 35.0 37.0 30.0 37.0 6 33.928606200065495 37.0 35.0 37.0 30.0 37.0 7 33.78546384292792 37.0 35.0 37.0 29.0 37.0 8 33.75344325820343 37.0 35.0 37.0 29.0 37.0 9 35.30712208227552 39.0 35.0 39.0 29.0 39.0 10 35.14219384359694 39.0 35.0 39.0 28.0 39.0 11 35.16357215041004 39.0 35.0 39.0 28.0 39.0 12 35.094053810427575 39.0 35.0 39.0 28.0 39.0 13 35.07118253009715 39.0 35.0 39.0 27.0 39.0 14 36.22085664637302 40.0 36.0 41.0 27.0 41.0 15 36.19507424374201 40.0 36.0 41.0 27.0 41.0 16 36.14971813082533 40.0 36.0 41.0 27.0 41.0 17 36.0753674334588 40.0 36.0 41.0 27.0 41.0 18 36.026663573910994 40.0 36.0 41.0 27.0 41.0 19 36.10454511843435 40.0 37.0 41.0 27.0 41.0 20 36.0656221720652 40.0 36.0 41.0 27.0 41.0 21 36.01641962767212 40.0 36.0 41.0 27.0 41.0 22 35.9550131691532 40.0 36.0 41.0 27.0 41.0 23 35.880668193678105 40.0 36.0 41.0 27.0 41.0 24 35.81164501737695 40.0 36.0 41.0 27.0 41.0 25 35.75482047345571 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5379493027213158 1101 2 0.6216999588084917 1101 3 0.5756597660214418 1101 4 0.6537627475051551 1101 5 0.6297365060287916 1101 6 0.5707223432088853 1101 7 0.6177284113687378 1101 8 0.7016691573766849 1101 9 0.7583691439587525 1101 10 0.8066424699292867 1101 11 0.7980439524498948 1101 12 0.8384877115472591 1101 13 0.8886176885678267 1101 14 0.9816304256242887 1101 15 0.9975245606778955 1101 16 0.9777383968568998 1101 17 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-0.752350115371307 2215 18 -0.769458671225614 2215 19 -0.8556738365695011 2215 20 -0.7737348933776573 2215 21 -0.8379808743365373 2215 22 -0.749244094876147 2215 23 -0.7107048113969299 2215 24 -0.8242165895874933 2215 25 -0.7738372693793778 2216 1 -0.3903678721392474 2216 2 -0.27432744266483766 2216 3 -0.3729292527861645 2216 4 -0.14315034669532167 2216 5 -0.5009043656060825 2216 6 -0.548612449657206 2216 7 -0.68609506877484 2216 8 -0.5803363534941184 2216 9 -0.7239691063397657 2216 10 -0.8292750246668845 2216 11 -0.7791796029993563 2216 12 -0.7150741246572139 2216 13 -0.8166732221680704 2216 14 -1.0195050568172732 2216 15 -0.9433729474565808 2216 16 -0.9400459111952841 2216 17 -1.0386602889349348 2216 18 -0.9597835464024556 2216 19 -0.9917462517350657 2216 20 -1.107736446031943 2216 21 -1.320489737733169 2216 22 -1.120509219134469 2216 23 -1.2329157149182493 2216 24 -1.0965707392596897 2216 25 -1.2121078988611558 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 58006.0 3 1.0 4 925.0 5 7539.0 6 8350.0 7 6476.0 8 5299.0 9 4573.0 10 4107.0 11 3833.0 12 3665.0 13 3382.0 14 3337.0 15 3249.0 16 3374.0 17 3596.0 18 3958.0 19 4689.0 20 6070.0 21 7845.0 22 10024.0 23 12053.0 24 14171.0 25 15898.0 26 17536.0 27 19430.0 28 21830.0 29 26318.0 30 33442.0 31 42505.0 32 54534.0 33 70971.0 34 94273.0 35 127032.0 36 180197.0 37 291756.0 38 1097732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.665848214088854 18.592587888842147 12.928948967541476 27.81261492952753 2 14.558745591593611 20.103345546871285 38.412119750188836 26.925789111346266 3 17.107955165698872 24.17287017702013 29.648187013828487 29.070987643452504 4 11.585932421006206 16.709002389609843 36.72996264391468 34.97510254546927 5 13.29507370587427 37.57937962313621 35.579691503120564 13.545855167868961 6 33.00803549699458 36.65181294468965 17.301251413930345 13.03890014438542 7 28.447144806938756 31.220314357416456 21.915863162065776 18.416677673579006 8 26.118538983564182 35.65968663828169 19.964615623008033 18.257158755146097 9 26.589332293365892 14.54962572616519 19.054512898207904 39.806529082261015 10 14.665656639461663 28.51881652512494 34.417704057333864 22.397822778079536 11 35.41190575956789 22.002213051546317 23.4501596847854 19.135721504100395 12 23.021546002246502 24.481992767529235 31.046454716070944 21.45000651415332 13 29.12649605453579 20.90963989056222 25.400708458187935 24.56315559671405 14 21.694856915487353 20.559529636293853 27.051062878310383 30.69455056990841 15 24.092023859406968 28.80875502206009 22.97555079807181 24.123670320461134 16 23.65812843093413 27.29654714662479 25.664707725785835 23.380616696655245 17 22.821043919640243 27.532263425392962 26.555963258325317 23.09072939664148 18 22.900524403865383 26.950598787236263 27.372111893370484 22.776764915527874 19 23.915173399719013 26.66749120589302 26.88012549428339 22.53720990010458 20 24.1509593411198 26.356660457680892 26.76647992760487 22.72590027359444 21 23.967893744421108 26.111333142015074 26.471463355804424 23.449309757759394 22 23.86802131891532 26.45528798097513 26.26756567969778 23.409125020411775 23 23.355233041298103 26.257905374950703 26.519187700715534 23.867673883035664 24 23.53695637629975 26.398738366954582 26.638485617805824 23.42581963893985 25 23.631101087513404 26.16951265244466 26.51744827841188 23.681937981630057 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 139.0 1 139.0 2 233.0 3 327.0 4 327.0 5 327.0 6 853.0 7 1379.0 8 1379.0 9 1379.0 10 2036.0 11 2693.0 12 2693.0 13 2693.0 14 4977.5 15 7262.0 16 7262.0 17 7262.0 18 14270.5 19 21279.0 20 21279.0 21 21279.0 22 33092.5 23 44906.0 24 44906.0 25 44906.0 26 64170.0 27 83434.0 28 83434.0 29 83434.0 30 107388.5 31 131343.0 32 131343.0 33 131343.0 34 157592.0 35 183841.0 36 183841.0 37 183841.0 38 207673.5 39 231506.0 40 231506.0 41 231506.0 42 251262.5 43 271019.0 44 271019.0 45 271019.0 46 288985.0 47 306951.0 48 306951.0 49 306951.0 50 304476.0 51 302001.0 52 302001.0 53 302001.0 54 277391.0 55 252781.0 56 252781.0 57 252781.0 58 220352.5 59 187924.0 60 187924.0 61 187924.0 62 154685.5 63 121447.0 64 121447.0 65 121447.0 66 94838.5 67 68230.0 68 68230.0 69 68230.0 70 50295.5 71 32361.0 72 32361.0 73 32361.0 74 22777.5 75 13194.0 76 13194.0 77 13194.0 78 9214.5 79 5235.0 80 5235.0 81 5235.0 82 3609.0 83 1983.0 84 1983.0 85 1983.0 86 1273.5 87 564.0 88 564.0 89 564.0 90 361.5 91 159.0 92 159.0 93 159.0 94 87.5 95 16.0 96 16.0 97 16.0 98 9.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18847030074261348 2 0.007922618900903882 3 0.006338095120723105 4 0.0025088293186195628 5 0.08239523656940038 6 0.3475828970024331 7 0.029753835427839027 8 0.015493121406212038 9 0.004841600439441262 10 0.003961309450451941 11 0.0 12 0.0 13 0.0 14 0.0014084655823829125 15 0.0 16 0.0 17 0.019410416307214514 18 0.02794923890041092 19 0.0 20 0.0 21 8.802909889893204E-4 22 1.3204364834839804E-4 23 0.004577513142744466 24 0.0 25 1.7605819779786407E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2271976.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.27626288775242 #Duplication Level Percentage of deduplicated Percentage of total 1 78.04865576639372 51.72773227609204 2 13.754989535068635 18.23258604888985 3 4.1629702041710255 8.27718322936558 4 1.6282041567049987 4.316451468988469 5 0.7943145258234913 2.632209916451906 6 0.4390491259607795 1.7459121175688728 7 0.2856937408076234 1.3254299429806013 8 0.18402323452316655 0.9757097814969522 9 0.12583101248297618 0.7505648336478407 >10 0.5359297984039093 5.943127392678553 >50 0.025950398943326163 1.166974544248248 >100 0.012260111642109113 1.442735827108796 >500 0.0013302431714054051 0.5838630842636545 >1k 7.981459028421716E-4 0.8795195362186558 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3134 0.1379415979746265 No Hit GTACATGGGAAGCAGTGGTATCAAC 2796 0.12306468026070698 No Hit CCCATGTACTCTGCGTTGATACCAC 2582 0.11364556667852126 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.401454944946602E-5 0.0 6 4.401454944946602E-5 0.0 0.0 8.802909889893203E-5 0.0 7 4.401454944946602E-5 0.0 0.0 8.802909889893203E-5 0.0 8 4.401454944946602E-5 0.0 0.0 8.802909889893203E-5 0.0 9 4.401454944946602E-5 0.0 0.0 8.802909889893203E-5 0.0 10 4.401454944946602E-5 4.401454944946602E-5 0.0 8.802909889893203E-5 0.0 11 4.401454944946602E-5 4.401454944946602E-5 0.0 1.3204364834839804E-4 0.0 12 4.401454944946602E-5 8.802909889893203E-5 0.0 1.7605819779786407E-4 0.0 13 4.401454944946602E-5 1.3204364834839804E-4 0.0 1.7605819779786407E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGGCC 55 1.9239473E-4 13.849104 4 ATTTGCG 55 1.9775859E-4 13.804284 16 CGTGCGC 110 1.9099389E-10 13.800334 10 TTGCGCG 65 5.5041117E-5 13.141195 18 CGACGGG 55 0.003091392 12.07928 14 GTCCTAC 735 0.0 10.88697 1 CAGTACG 70 0.001464296 10.881439 4 CTAGACA 255 0.0 10.828097 4 TAGGACA 415 0.0 10.783113 4 AAGGCGT 170 1.3460522E-10 10.639527 6 GTCCTAA 405 0.0 10.584554 1 TATGGCG 660 0.0 10.209418 16 TAGGACG 595 0.0 10.081333 4 TTAGGAC 510 0.0 10.081332 3 AAAACGC 85 6.654038E-4 10.049149 18 GTAGGAC 590 0.0 10.005391 3 TAGACTG 220 1.8189894E-12 9.954043 5 GTACTAG 115 9.938638E-6 9.940276 1 GTTCTAG 250 0.0 9.907142 1 GACGTGA 365 0.0 9.880028 7 >>END_MODULE