FastQCFastQC Report
Thu 26 May 2016
SRR1513192_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513192_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164532
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACCGT456.838202E-414.7564816
TAGGACC6050.011.9728474
TGGCGAG11200.010.92947918
CTACACT3500.010.6202744
CGCAGAT1451.4768375E-810.4676518
TAGGACT3950.010.3755364
CTAGGAC3050.010.3122463
TGTAGGA13450.010.2824732
GTATTAA2700.010.2605991
GTCCTAC13000.010.2143311
GGCGAGA7000.010.167191519
TATGGCG12450.010.13724916
GTAGGAC13000.010.1175393
TGTACCG1054.048511E-59.9652095
ACCGTGC1054.1718056E-59.9380398
CTTATAC2400.09.9281413
GGACGTG9750.09.9241396
CTAATAC2500.09.9122573
GACGTGG4600.09.8987587
AGGACGT10200.09.8852745
Produced by FastQC (version 0.11.5)