##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513192_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2164532 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.313167465299657 31.0 30.0 34.0 24.0 34.0 2 29.189153128713272 31.0 30.0 34.0 20.0 34.0 3 29.37029944579244 31.0 30.0 34.0 23.0 34.0 4 32.426008023905396 37.0 33.0 37.0 20.0 37.0 5 32.56568625458067 37.0 33.0 37.0 23.0 37.0 6 32.93301092337743 37.0 35.0 37.0 26.0 37.0 7 32.89633971685334 37.0 35.0 37.0 26.0 37.0 8 32.78141649095509 37.0 35.0 37.0 25.0 37.0 9 34.01352671154781 39.0 35.0 39.0 23.0 39.0 10 33.600824104240544 39.0 34.0 39.0 17.0 39.0 11 34.04499633177056 39.0 34.0 39.0 25.0 39.0 12 34.031998140937624 39.0 35.0 39.0 25.0 39.0 13 34.07049145034585 39.0 35.0 39.0 25.0 39.0 14 34.30309877608647 39.0 34.0 41.0 16.0 41.0 15 34.72906429657774 40.0 34.0 41.0 18.0 41.0 16 34.56816716038386 39.0 34.0 41.0 17.0 41.0 17 34.18397926202985 39.0 33.0 41.0 16.0 41.0 18 33.911231157589725 39.0 33.0 41.0 11.0 41.0 19 34.54115716468964 39.0 34.0 41.0 18.0 41.0 20 34.751169305882286 40.0 34.0 41.0 18.0 41.0 21 34.79647563537984 40.0 35.0 41.0 18.0 41.0 22 34.48274361386203 40.0 34.0 41.0 16.0 41.0 23 34.028084592881974 39.0 34.0 41.0 10.0 41.0 24 34.2727462564656 39.0 34.0 41.0 8.0 41.0 25 33.91975863604696 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9692533701981709 1101 2 1.1659813880888628 1101 3 1.1442012423480818 1101 4 1.5200564601668063 1101 5 1.4237207295415644 1101 6 1.1788951594373813 1101 7 1.097350325628888 1101 8 1.1312602850078335 1101 9 1.4480356250675257 1101 10 1.8122291451544825 1101 11 1.3375702156730895 1101 12 1.2301773682879684 1101 13 1.1521140480479275 1101 14 2.1444001899459977 1101 15 1.7676402350994067 1101 16 1.9437813306658 1101 17 2.278709046222353 1101 18 2.272050778644825 1101 19 1.8083252859607626 1101 20 1.4966365287847694 1101 21 1.4239576564725098 1101 22 1.7200199016550428 1101 23 1.9056198989773634 1101 24 1.6303563875848113 1101 25 1.8339698720149045 1102 1 0.9676138449558245 1102 2 1.176813588082755 1102 3 1.1065946321017535 1102 4 1.3906590444156208 1102 5 1.4466793679369943 1102 6 1.103488449128342 1102 7 1.0632817309932818 1102 8 1.099228765252498 1102 9 1.4601511998526604 1102 10 1.5879908901918682 1102 11 1.363233307329132 1102 12 1.163065175904478 1102 13 1.218992819484491 1102 14 2.1417940964554774 1102 15 1.646394054981819 1102 16 1.7448717441409158 1102 17 2.1489460844599364 1102 18 2.298154287181866 1102 19 1.7408366839496452 1102 20 1.6033489518292612 1102 21 1.5299290047118959 1102 22 1.7200988830823718 1102 23 1.9839010634630867 1102 24 1.8053099382844309 1102 25 2.000409870793483 1103 1 0.8630147206501206 1103 2 1.0810864021325983 1103 3 0.8997778546475388 1103 4 1.3244856763127473 1103 5 1.2204416254667407 1103 6 0.9348968343969375 1103 7 0.8938277948650111 1103 8 0.9545141152420058 1103 9 1.2955815420374677 1103 10 1.590453636293553 1103 11 1.2446200192791963 1103 12 1.1049031540144227 1103 13 1.152263207967195 1103 14 2.085458455144284 1103 15 1.6286599773775094 1103 16 1.5813554903048512 1103 17 2.0404454746096405 1103 18 2.2482903794825333 1103 19 1.5937154885969562 1103 20 1.2971556857754436 1103 21 1.3337797078266291 1103 22 1.6497917329623561 1103 23 1.9856087019244626 1103 24 1.5082021734722133 1103 25 1.805294667882798 1104 1 1.0135692975787052 1104 2 1.2548156702947928 1104 3 0.9863679536920635 1104 4 1.4413783046051378 1104 5 1.341346893584081 1104 6 1.1259789215132088 1104 7 1.0097361262003872 1104 8 1.056487013911017 1104 9 1.3691699506341735 1104 10 1.456101377907899 1104 11 1.1946155862368144 1104 12 1.1619948482414983 1104 13 1.0123074509655794 1104 14 2.056850174829286 1104 15 1.689117457849079 1104 16 1.7746839373136538 1104 17 2.2266926471790995 1104 18 2.3756426121156764 1104 19 1.6508007209401683 1104 20 1.5113293148844065 1104 21 1.4969262157438195 1104 22 1.7688249553128372 1104 23 2.028447761009552 1104 24 1.7659572180891487 1104 25 2.0254725274350847 1105 1 0.9040778118907085 1105 2 1.1117880857303817 1105 3 0.9338436757635407 1105 4 1.2975864506505062 1105 5 1.1714848025762237 1105 6 0.9409933957053127 1105 7 0.9350389749804435 1105 8 0.8709662229317843 1105 9 1.1295000104757094 1105 10 1.499342734715455 1105 11 1.0458224556385005 1105 12 1.0157066480057821 1105 13 0.9421384027408735 1105 14 1.812923674787271 1105 15 1.3858083802687204 1105 16 1.6076482028314985 1105 17 1.8347010420292946 1105 18 1.5707931211087853 1105 19 1.3693982193671346 1105 20 1.2202420617971654 1105 21 1.0712164338109602 1105 22 1.358982275774629 1105 23 1.6519033059113113 1105 24 1.482595256843986 1105 25 1.6315051528582885 1106 1 0.39881986977098904 1106 2 0.5936589054297663 1106 3 0.5141841063970638 1106 4 1.0294514307456595 1106 5 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1107 18 1.9024901610506788 1107 19 1.331946123455424 1107 20 1.151981243588743 1107 21 1.028453118094717 1107 22 1.296889823514583 1107 23 1.6285810747949654 1107 24 1.470369350717057 1107 25 1.7325524423545602 1108 1 0.46707508713490853 1108 2 0.7650426226644598 1108 3 0.5295842617578437 1108 4 1.0333219665666036 1108 5 0.8583201087438752 1108 6 0.5113906273768123 1108 7 0.498569028810266 1108 8 0.522453543349755 1108 9 0.7477064895019652 1108 10 0.8675980137013539 1108 11 0.5609447772152478 1108 12 0.2671295232336064 1108 13 0.3670894565641234 1108 14 1.3653428733859272 1108 15 0.893991969006791 1108 16 0.9312298603340068 1108 17 1.3066175791110481 1108 18 1.6018924032558957 1108 19 0.8506763067596381 1108 20 0.6053871530329857 1108 21 0.6935699955500425 1108 22 0.8994174288529706 1108 23 1.2798324001182593 1108 24 1.0181750880948215 1108 25 1.2496411735158475 1109 1 0.4190133925693189 1109 2 0.5780590392290854 1109 3 0.5130643699619135 1109 4 0.7081649583881386 1109 5 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-2.2586690190917835 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 107868.0 3 7.0 4 942.0 5 5356.0 6 6083.0 7 5621.0 8 5149.0 9 4759.0 10 4429.0 11 4303.0 12 4149.0 13 4149.0 14 4051.0 15 4154.0 16 4339.0 17 4531.0 18 5149.0 19 6357.0 20 8182.0 21 10215.0 22 12628.0 23 15269.0 24 17370.0 25 20090.0 26 22699.0 27 25351.0 28 28199.0 29 33527.0 30 40704.0 31 48269.0 32 61921.0 33 80319.0 34 105872.0 35 142072.0 36 202447.0 37 294598.0 38 813404.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9756390469425 18.105038098697957 12.109392896727423 25.80992995763212 2 14.689105898636209 19.88643890833865 41.084509351790174 24.33994584123496 3 18.710161281954356 24.31329167871093 29.508028891408923 27.468518147925792 4 11.483888995648837 16.359801445065507 38.78584233134017 33.37046722794548 5 12.84844077155404 37.77515796105171 36.11073023402324 13.26567103337101 6 32.40587443517268 36.79340269675444 17.948281940727963 12.852440927344919 7 28.417510191143432 31.259617010776598 22.128897748475833 18.193975049604138 8 25.42937161039133 36.222241424004146 20.526294144909482 17.822092820695044 9 26.669029414360484 14.917101287481858 19.748750893539118 38.66511840461854 10 13.923139909282803 28.55909741193519 35.51810277585755 21.999659902924463 11 34.85865347204445 22.217175250472017 24.209861402865098 18.714309874618433 12 23.337176604873946 24.415757259277573 31.429026943721105 20.818039192127376 13 29.03860834718122 21.38639400957861 25.63248964412002 23.942507999120146 14 20.71127676957372 21.192740930784502 28.4125776793603 29.68340462028148 15 23.517264743005807 29.709518983106154 23.219901954395436 23.553314319492607 16 22.730814017966065 27.605125503678234 27.08203948098037 22.58202099737533 17 22.0100846239526 27.87021246111966 27.842759360167125 22.276943554760617 18 21.909187831263665 27.064481140991177 29.079248181385875 21.947082846359283 19 23.62158113072053 26.839305114688976 27.187921531731075 22.351192222859424 20 23.904264084798314 26.890985812263075 27.299875087284086 21.904875015654525 21 23.550047831853703 26.47911342388265 26.708382821201297 23.262455923062348 22 23.46843555317683 27.187247674153653 26.807652541734434 22.536664230935084 23 22.733786552146583 26.990026012868263 27.37848607787039 22.897701357114766 24 23.260368274874548 26.6990733272723 27.35785263735893 22.682705760494226 25 23.134404234060117 26.678285190864532 27.350717677256437 22.836592897818917 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 160.0 1 160.0 2 281.0 3 402.0 4 402.0 5 402.0 6 790.0 7 1178.0 8 1178.0 9 1178.0 10 1945.0 11 2712.0 12 2712.0 13 2712.0 14 5219.0 15 7726.0 16 7726.0 17 7726.0 18 14852.0 19 21978.0 20 21978.0 21 21978.0 22 35115.0 23 48252.0 24 48252.0 25 48252.0 26 69416.0 27 90580.0 28 90580.0 29 90580.0 30 117194.5 31 143809.0 32 143809.0 33 143809.0 34 168951.0 35 194093.0 36 194093.0 37 194093.0 38 215480.5 39 236868.0 40 236868.0 41 236868.0 42 253569.0 43 270270.0 44 270270.0 45 270270.0 46 280696.0 47 291122.0 48 291122.0 49 291122.0 50 283745.5 51 276369.0 52 276369.0 53 276369.0 54 248842.0 55 221315.0 56 221315.0 57 221315.0 58 190939.0 59 160563.0 60 160563.0 61 160563.0 62 131084.5 63 101606.0 64 101606.0 65 101606.0 66 78092.5 67 54579.0 68 54579.0 69 54579.0 70 39913.5 71 25248.0 72 25248.0 73 25248.0 74 17706.0 75 10164.0 76 10164.0 77 10164.0 78 6922.0 79 3680.0 80 3680.0 81 3680.0 82 2483.5 83 1287.0 84 1287.0 85 1287.0 86 859.5 87 432.0 88 432.0 89 432.0 90 274.0 91 116.0 92 116.0 93 116.0 94 65.0 95 14.0 96 14.0 97 14.0 98 11.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.170799045705954 2 0.2630591739923457 3 0.2692498886595347 4 0.4696627261689825 5 0.26666272432100796 6 0.017324761195491683 7 0.018387346548815173 8 0.01760195737461955 9 0.01778675482737146 10 0.020512517255462152 11 0.01931133381257473 12 0.026657032559463202 13 0.02513245357425993 14 0.023469276499492733 15 0.039038461893841254 16 0.020882112160965974 17 0.038899863804277325 18 0.03044538034087738 19 0.025363450390199823 20 0.028689804539734225 21 0.032431952957960426 22 0.04208761986424779 23 0.027858216002350625 24 0.045506372740158146 25 0.03446472493823145 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2164532.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.36747808838433 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13318884279201 60.99272410841059 2 11.43248314109518 16.775449127002474 3 2.9017958810909263 6.386923371687073 4 1.036668520756832 3.042310199261787 5 0.47259430974318045 1.7336526332388955 6 0.2840594701091829 1.2504436169420123 7 0.1765221024991017 0.9065687041052862 8 0.11014495099928216 0.6464845823188801 9 0.08106585117132666 0.535283735357568 >10 0.3443713085677144 4.318500564670868 >50 0.016338679561048956 0.8200071429528256 >100 0.008921231314505113 1.27130819699568 >500 0.0011456132894486454 0.5142038589555755 >1k 7.000970102179244E-4 0.8061401581004013 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.6199363187977816E-5 0.0 5 0.0 0.0 0.0 4.6199363187977816E-5 0.0 6 0.0 0.0 0.0 4.6199363187977816E-5 0.0 7 0.0 0.0 0.0 4.6199363187977816E-5 0.0 8 0.0 0.0 0.0 9.239872637595563E-5 0.0 9 0.0 0.0 0.0 9.239872637595563E-5 0.0 10 0.0 0.0 0.0 9.239872637595563E-5 0.0 11 0.0 0.0 0.0 9.239872637595563E-5 0.0 12 0.0 0.0 0.0 9.239872637595563E-5 0.0 13 0.0 0.0 0.0 9.239872637595563E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACCGT 45 6.838202E-4 14.756481 6 TAGGACC 605 0.0 11.972847 4 TGGCGAG 1120 0.0 10.929479 18 CTACACT 350 0.0 10.620274 4 CGCAGAT 145 1.4768375E-8 10.467651 8 TAGGACT 395 0.0 10.375536 4 CTAGGAC 305 0.0 10.312246 3 TGTAGGA 1345 0.0 10.282473 2 GTATTAA 270 0.0 10.260599 1 GTCCTAC 1300 0.0 10.214331 1 GGCGAGA 700 0.0 10.1671915 19 TATGGCG 1245 0.0 10.137249 16 GTAGGAC 1300 0.0 10.117539 3 TGTACCG 105 4.048511E-5 9.965209 5 ACCGTGC 105 4.1718056E-5 9.938039 8 CTTATAC 240 0.0 9.928141 3 GGACGTG 975 0.0 9.924139 6 CTAATAC 250 0.0 9.912257 3 GACGTGG 460 0.0 9.898758 7 AGGACGT 1020 0.0 9.885274 5 >>END_MODULE