FastQCFastQC Report
Thu 26 May 2016
SRR1513192_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513192_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164532
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27500.127048248766939No Hit
GTACATGGGAAGCAGTGGTATCAAC22780.10524214934221347No Hit
CCCATGTACTCTGCGTTGATACCAC22410.1035327729042583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4750.013.6266084
AAAGGCG1650.012.1145655
GGTTCGT550.00309503412.0773388
CGTGCGC951.0560652E-611.98514810
AAGGCGT1459.458745E-1111.8145336
GCGTGCG1052.765646E-711.7473479
GTCCTAC11550.011.13258651
GTAGGAC12200.011.0795243
GTATTAG1900.011.0284221
TGTAGGA13250.010.921462
GTCCTAA7400.010.8116461
TGGCGAG12300.010.34108418
GGCGAGG5650.010.24465219
ATAGGAC2053.6379788E-1210.2155423
CTGTAGG13150.010.1402021
AGGACGT10850.010.0010955
TATGGCG13050.09.96474916
TCCTACA13650.09.90395452
AGGACCT10100.09.8013175
TGCGCCA1651.0362783E-89.77983112
Produced by FastQC (version 0.11.5)