FastQCFastQC Report
Thu 26 May 2016
SRR1513191_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513191_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624547
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28270.10771382642414101No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACT551.9169506E-413.8552064
AACCGTT600.005927703311.0706717
TCCAACG2001.8189894E-1210.43985218
TAGGACC4150.010.3288054
GCGTTTA750.002670884810.12310713
ATTTGCG750.002671307210.12291416
GACGTGA4850.09.9782787
CCAACGA1252.6030084E-69.87059319
GACCGAG1356.5486347E-79.8405967
GTCCTAA5900.09.5473721
GGCGAGG5500.09.49095619
AACCGTG800.00455998869.4891477
ACCGAGC800.00455998869.4891478
ACCGTGA800.00455998869.4891478
GGACGTG9650.09.4394416
GTCCTAC11900.09.3869111
CTACACC2950.09.3640054
TTAGGAC8250.09.3524423
GTAGAAC6650.09.3107713
AACAGTG7250.09.292827
Produced by FastQC (version 0.11.5)