##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513191_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2624547 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.257468812713203 31.0 30.0 34.0 23.0 34.0 2 29.155136486410797 31.0 30.0 34.0 20.0 34.0 3 29.32693146664929 31.0 30.0 34.0 22.0 34.0 4 32.39313260536009 35.0 33.0 37.0 19.0 37.0 5 32.51866779295627 37.0 33.0 37.0 22.0 37.0 6 32.87690751965958 37.0 35.0 37.0 26.0 37.0 7 32.836966531748146 37.0 35.0 37.0 25.0 37.0 8 32.740198594271696 37.0 35.0 37.0 25.0 37.0 9 33.951166048845764 39.0 35.0 39.0 22.0 39.0 10 33.55840379311172 39.0 34.0 39.0 17.0 39.0 11 33.968055820680675 39.0 34.0 39.0 24.0 39.0 12 33.962025827695214 39.0 35.0 39.0 23.0 39.0 13 33.99415937302704 39.0 35.0 39.0 23.0 39.0 14 34.249286448289936 39.0 33.0 41.0 16.0 41.0 15 34.65272597518734 40.0 34.0 41.0 17.0 41.0 16 34.48107120962208 39.0 34.0 41.0 17.0 41.0 17 34.117520471151785 39.0 33.0 41.0 13.0 41.0 18 33.866886742740746 39.0 33.0 41.0 11.0 41.0 19 34.48239067541942 39.0 34.0 41.0 18.0 41.0 20 34.683439465934505 40.0 34.0 41.0 18.0 41.0 21 34.73088498700918 40.0 35.0 41.0 18.0 41.0 22 34.4245445023465 40.0 34.0 41.0 11.0 41.0 23 33.96790455648156 39.0 34.0 41.0 10.0 41.0 24 34.19862818231108 39.0 34.0 41.0 2.0 41.0 25 33.839066703701626 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0581930639001698 1101 2 1.3196491698922515 1101 3 1.2025147813182713 1101 4 1.6776925451640707 1101 5 1.483037079748847 1101 6 1.236220910765013 1101 7 1.1922493348349903 1101 8 1.2009379546679284 1101 9 1.662657004680284 1101 10 2.021060849537136 1101 11 1.5720909348823682 1101 12 1.447895086028673 1101 13 1.3606750285525564 1101 14 2.3391029669127335 1101 15 1.9414702570483655 1101 16 1.9964376104559634 1101 17 2.3794488605949766 1101 18 2.4031322955062038 1101 19 2.0005826389930093 1101 20 1.4535147947229063 1101 21 1.4960465032748615 1101 22 1.955192441262021 1101 23 2.1374793763507682 1101 24 1.8627302871662081 1101 25 2.104358132308022 1102 1 1.141147190625027 1102 2 1.3687568381703379 1102 3 1.2216049054472506 1102 4 1.5540167434404495 1102 5 1.5336852233138814 1102 6 1.1754884562890524 1102 7 1.1989799084021726 1102 8 1.2371404988445391 1102 9 1.58128845105513 1102 10 1.7458954450592543 1102 11 1.4100000462904632 1102 12 1.3839298883407594 1102 13 1.2803360249942273 1102 14 2.3164275666696454 1102 15 1.867639637833399 1102 16 2.024262410953135 1102 17 2.2723784035121426 1102 18 2.5406690749723992 1102 19 1.8855227193991055 1102 20 1.6944993569716331 1102 21 1.733410041143273 1102 22 1.939750290206348 1102 23 2.1442401984508948 1102 24 1.984828215416691 1102 25 2.1592619694719204 1103 1 0.5411642195855606 1103 2 0.8584087970903127 1103 3 0.6369129928907817 1103 4 1.129281771072705 1103 5 0.9448484423149424 1103 6 0.7061097726823959 1103 7 0.7666346558903996 1103 8 0.6846890517414579 1103 9 1.0967319922718417 1103 10 1.2954949363053814 1103 11 0.9020376398175642 1103 12 0.8587883480208376 1103 13 0.8655301156410502 1103 14 1.693588776958343 1103 15 1.3572906851387785 1103 16 1.4335004822635184 1103 17 1.7658413976118084 1103 18 1.9444826748278032 1103 19 1.3565227399408215 1103 20 0.9868272328938374 1103 21 1.0740955461431128 1103 22 1.2594592396240145 1103 23 1.6396353292864276 1103 24 1.40267095606562 1103 25 1.5999892142651078 1104 1 0.7449694456397467 1104 2 0.9484576789603487 1104 3 0.8172448293493666 1104 4 1.266208439704755 1104 5 1.1747331124173712 1104 6 0.8771337475515395 1104 7 0.7729449554569925 1104 8 0.7937123807240312 1104 9 1.1427185656600898 1104 10 1.2917172949901712 1104 11 0.9455486816644054 1104 12 0.8460891537888031 1104 13 0.9060268978292569 1104 14 1.891882743423622 1104 15 1.511980641235624 1104 16 1.4847702365452733 1104 17 1.8894259212888116 1104 18 1.9893502619643897 1104 19 1.476057261232448 1104 20 1.2258357236110555 1104 21 1.105900872505437 1104 22 1.4072136216830486 1104 23 1.6660397350157368 1104 24 1.526733411339805 1104 25 1.7591034322102388 1105 1 0.5920197054877292 1105 2 0.8293042586160269 1105 3 0.7710892252662838 1105 4 1.0150640928796406 1105 5 0.9621986211946449 1105 6 0.6690513748939821 1105 7 0.5270602026934625 1105 8 0.6646940474753364 1105 9 0.9739367194697834 1105 10 1.1343856883339996 1105 11 0.6325740570474068 1105 12 0.6648142904840739 1105 13 0.5597595675768616 1105 14 1.2509105564684972 1105 15 1.0408043000983866 1105 16 1.1652308530705895 1105 17 1.5185035032636591 1105 18 1.136376822321914 1105 19 0.9396260659317193 1105 20 0.724376512126959 1105 21 0.8045152987238495 1105 22 0.9806363359518357 1105 23 1.2604940542478715 1105 24 1.0850663214679557 1105 25 1.3193416120893673 1106 1 0.6359370063216438 1106 2 0.8096823145447871 1106 3 0.7550319705616708 1106 4 1.2286406501863354 1106 5 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1107 18 1.607585819510291 1107 19 1.1113759655166717 1107 20 0.8229021514361037 1107 21 0.7155993349625192 1107 22 1.0760286687049927 1107 23 1.28551070083153 1107 24 0.8857487867689784 1107 25 1.1598596644837116 1108 1 0.5288341551608262 1108 2 0.7240428101629526 1108 3 0.5622543693061708 1108 4 1.0783934044478798 1108 5 0.8944929334674896 1108 6 0.5630347362163874 1108 7 0.45153585575108934 1108 8 0.5200450361089679 1108 9 0.7218265351749764 1108 10 1.0029517072231613 1108 11 0.6592108852697649 1108 12 0.5006577660842453 1108 13 0.5252865093208996 1108 14 1.5111276323932472 1108 15 0.9688730967741961 1108 16 1.016524757487467 1108 17 1.3100388768707276 1108 18 1.5371031183117552 1108 19 1.0684127104059087 1108 20 0.7784286090710211 1108 21 0.8158793586264963 1108 22 1.07592980589029 1108 23 1.3080156636922453 1108 24 1.108124737162278 1108 25 1.4082705411462157 1109 1 0.6150027815480463 1109 2 0.7384001581773987 1109 3 0.6955625247150223 1109 4 0.9490487660520088 1109 5 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2216 25 -1.5018564509548256 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135027.0 3 2.0 4 1074.0 5 6663.0 6 7513.0 7 6825.0 8 6248.0 9 5989.0 10 5582.0 11 5283.0 12 5106.0 13 4970.0 14 4973.0 15 5009.0 16 5211.0 17 5570.0 18 6374.0 19 7780.0 20 9592.0 21 12466.0 22 15262.0 23 18398.0 24 21303.0 25 24224.0 26 27146.0 27 30300.0 28 34246.0 29 40358.0 30 49174.0 31 58157.0 32 74771.0 33 96876.0 34 127770.0 35 171900.0 36 244811.0 37 358456.0 38 984138.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.88000983159951 18.950255388773734 13.314756597304783 24.854978182321975 2 14.2388889567965 20.284835076182155 41.51270313712788 23.963572829893472 3 18.213373854609294 25.127049174065775 29.972603828129895 26.686973143195036 4 11.473035737447624 17.178485217071238 39.40164272194289 31.946836323538246 5 12.918566165587286 37.86914895445595 36.040664396498876 13.171620483457888 6 31.14028351891492 37.411021636809366 18.620910158290886 12.827784685984833 7 27.70335852676178 31.75587640243482 22.530264696769976 18.01050037403343 8 25.13131961349505 36.13552976755742 21.022875373647363 17.71027524530016 9 26.249795643364234 15.450238158842936 20.438369277871292 37.86159691992154 10 14.02507969596442 29.05620542408924 35.291716764448644 21.6269981154977 11 34.0489369032085 22.87247259179766 24.42996121998512 18.64862928500872 12 22.76361443872931 24.95723811585498 31.73819817732233 20.540949268093385 13 28.4245079588668 21.841321165029008 26.08894661541405 23.645224260690142 14 20.661370257279575 21.645205105651247 28.840709839796702 28.852714797272473 15 23.336633010468123 29.634620156592995 23.800900251535264 23.227846581403618 16 22.32497784891531 28.22480730938158 27.31075351797334 22.139461323729765 17 21.672712053571427 28.082499329137395 28.057113278200625 22.187675339090553 18 21.491235091041172 27.773140663125183 29.236461594400094 21.499162651433554 19 23.220821906113606 27.261941603413607 27.567370415709426 21.949866074763364 20 23.418844398167078 27.396406652506506 27.7219277587828 21.462821190543615 21 23.511370254390005 26.80368245004742 27.13459069828425 22.550356597278334 22 23.07329596459563 27.398666999567357 27.40122093233793 22.126816103499078 23 22.653773856729277 27.054967855634676 27.613282780468545 22.6779755071675 24 22.801708840662084 27.204676249257137 27.655863856922142 22.337751053158637 25 22.97903027604902 26.97080948562124 27.528098881228484 22.522061357101254 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 213.0 1 213.0 2 339.0 3 465.0 4 465.0 5 465.0 6 1005.5 7 1546.0 8 1546.0 9 1546.0 10 2703.5 11 3861.0 12 3861.0 13 3861.0 14 7638.0 15 11415.0 16 11415.0 17 11415.0 18 21303.5 19 31192.0 20 31192.0 21 31192.0 22 49631.5 23 68071.0 24 68071.0 25 68071.0 26 95886.5 27 123702.0 28 123702.0 29 123702.0 30 158173.5 31 192645.0 32 192645.0 33 192645.0 34 221593.5 35 250542.0 36 250542.0 37 250542.0 38 274406.5 39 298271.0 40 298271.0 41 298271.0 42 314443.0 43 330615.0 44 330615.0 45 330615.0 46 338544.5 47 346474.0 48 346474.0 49 346474.0 50 333934.0 51 321394.0 52 321394.0 53 321394.0 54 287161.0 55 252928.0 56 252928.0 57 252928.0 58 215200.0 59 177472.0 60 177472.0 61 177472.0 62 143844.5 63 110217.0 64 110217.0 65 110217.0 66 84707.0 67 59197.0 68 59197.0 69 59197.0 70 43251.5 71 27306.0 72 27306.0 73 27306.0 74 19045.5 75 10785.0 76 10785.0 77 10785.0 78 7454.5 79 4124.0 80 4124.0 81 4124.0 82 2778.0 83 1432.0 84 1432.0 85 1432.0 86 961.0 87 490.0 88 490.0 89 490.0 90 320.0 91 150.0 92 150.0 93 150.0 94 84.5 95 19.0 96 19.0 97 19.0 98 20.0 99 21.0 100 21.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16856242239136887 2 0.25189108825256323 3 0.25387238254830263 4 0.4175196710137026 5 0.24888104499557448 6 0.017298223274340295 7 0.01714581602082188 8 0.01581225255253573 9 0.017450630527858713 10 0.019508128450357338 11 0.01962243389049615 12 0.027242796566416982 13 0.025947334911510442 14 0.024042244242530236 15 0.04031171855562122 16 0.019889146584153382 17 0.040349820369000824 18 0.030062330756507695 19 0.0264807602988249 20 0.02872876728822155 21 0.032729457693079986 22 0.043512270879507965 23 0.02891927635511957 24 0.04755106309774601 25 0.03638723177752199 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2624547.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.43612095506711 #Duplication Level Percentage of deduplicated Percentage of total 1 82.63448316708644 59.85721417750529 2 11.63606881044276 16.85743375589431 3 2.9795239478147986 6.474754712172953 4 1.1280280491188708 3.268399048267316 5 0.5282504643115388 1.913220726372049 6 0.3038155831580193 1.320433339780112 7 0.1943641480258881 0.985528945501226 8 0.11919855247733926 0.6907424611933969 9 0.08939517568558379 0.5827895782884379 >10 0.3575324079619851 4.377646166002222 >50 0.01778391181400496 0.9046280857322836 >100 0.009378758749625337 1.2647590658937269 >500 0.0015398457864532187 0.7666675524913851 >1k 6.371775667599164E-4 0.7357823849052453 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2827 0.10771382642414101 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.8101813379604175E-5 0.0 8 3.8101813379604175E-5 0.0 0.0 3.8101813379604175E-5 0.0 9 3.8101813379604175E-5 0.0 0.0 3.8101813379604175E-5 0.0 10 3.8101813379604175E-5 0.0 0.0 3.8101813379604175E-5 0.0 11 3.8101813379604175E-5 0.0 0.0 1.524072535184167E-4 0.0 12 3.8101813379604175E-5 0.0 0.0 1.524072535184167E-4 0.0 13 3.8101813379604175E-5 0.0 0.0 1.524072535184167E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGACT 55 1.9169506E-4 13.855206 4 AACCGTT 60 0.0059277033 11.070671 7 TCCAACG 200 1.8189894E-12 10.439852 18 TAGGACC 415 0.0 10.328805 4 GCGTTTA 75 0.0026708848 10.123107 13 ATTTGCG 75 0.0026713072 10.122914 16 GACGTGA 485 0.0 9.978278 7 CCAACGA 125 2.6030084E-6 9.870593 19 GACCGAG 135 6.5486347E-7 9.840596 7 GTCCTAA 590 0.0 9.547372 1 GGCGAGG 550 0.0 9.490956 19 AACCGTG 80 0.0045599886 9.489147 7 ACCGAGC 80 0.0045599886 9.489147 8 ACCGTGA 80 0.0045599886 9.489147 8 GGACGTG 965 0.0 9.439441 6 GTCCTAC 1190 0.0 9.386911 1 CTACACC 295 0.0 9.364005 4 TTAGGAC 825 0.0 9.352442 3 GTAGAAC 665 0.0 9.310771 3 AACAGTG 725 0.0 9.29282 7 >>END_MODULE