FastQCFastQC Report
Thu 26 May 2016
SRR1513191_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513191_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2624547
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40240.1533216970395272No Hit
TATCAACGCAGAGTACTTTTTTTTT28340.10798053911779823No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG500.001510304413.2879537
GATCGAT500.001512257513.28567411
TAGGACC4900.011.4629334
ACCGCAT600.00592733911.0707638
AAGGCGT1053.3866472E-610.8788236
GGCGAGG5100.010.41993819
GTCCTAT2750.010.3912831
GAACAGT5200.010.2511996
GCGTGCG750.002675350810.1210699
GATATAC6650.09.8834391
TTAGGAC7800.09.76513
TGTAGGA12000.09.7603032
CTAGCAC2350.09.7235453
TTAAGGT2650.09.6996864
GTAGGAC10800.09.609133
TAAAGCG900.00109275959.5200624
TGGACCG1002.6986666E-49.5200625
TAAGGTG2201.6370905E-119.5200625
ACGAAAT1002.7832558E-49.49085312
CGAAATC1201.720761E-59.49085213
Produced by FastQC (version 0.11.5)