##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513191_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2624547 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.50457431320529 31.0 31.0 34.0 27.0 34.0 2 30.626738252353643 31.0 31.0 34.0 27.0 34.0 3 30.72106500664686 33.0 31.0 34.0 27.0 34.0 4 34.08484435599743 37.0 35.0 37.0 30.0 37.0 5 33.80780911905941 37.0 35.0 37.0 29.0 37.0 6 33.71756002083407 37.0 35.0 37.0 29.0 37.0 7 33.57628954634838 37.0 35.0 37.0 28.0 37.0 8 33.539410420160124 37.0 35.0 37.0 28.0 37.0 9 35.06279216946772 39.0 35.0 39.0 28.0 39.0 10 34.90272721349627 39.0 35.0 39.0 27.0 39.0 11 34.907901058735085 39.0 35.0 39.0 27.0 39.0 12 34.847541690051656 39.0 35.0 39.0 27.0 39.0 13 34.8136253608718 39.0 35.0 39.0 27.0 39.0 14 35.9505960457176 40.0 36.0 41.0 27.0 41.0 15 35.92350298927777 40.0 36.0 41.0 27.0 41.0 16 35.88898007922891 40.0 36.0 41.0 27.0 41.0 17 35.81778112565711 40.0 36.0 41.0 27.0 41.0 18 35.77510671365382 40.0 36.0 41.0 27.0 41.0 19 35.85982457163084 40.0 36.0 41.0 27.0 41.0 20 35.81846695829795 40.0 36.0 41.0 27.0 41.0 21 35.76640044929658 40.0 36.0 41.0 27.0 41.0 22 35.70847997768758 40.0 36.0 41.0 27.0 41.0 23 35.63199211140056 40.0 36.0 41.0 26.0 41.0 24 35.55891169028408 40.0 36.0 41.0 26.0 41.0 25 35.502647885520815 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7983724298068857 1101 2 0.7858787897107185 1101 3 0.7195960311018545 1101 4 0.94496192822551 1101 5 0.8441255198143551 1101 6 0.7734290601585059 1101 7 0.8331768859092819 1101 8 0.8815882675980689 1101 9 0.9776586663342428 1101 10 1.0497348698041833 1101 11 0.9489715003941583 1101 12 0.8851016069256019 1101 13 0.9529235121685744 1101 14 1.1459983215331064 1101 15 1.138806085906623 1101 16 1.2077605692106061 1101 17 1.196117634482988 1101 18 1.2026362324564985 1101 19 1.1841054129291066 1101 20 1.241182843786028 1101 21 1.1544963311669463 1101 22 1.2370610887626015 1101 23 1.4309656634004995 1101 24 1.428101950908335 1101 25 1.3136925922717637 1102 1 0.6672972869513139 1102 2 0.703630278763498 1102 3 0.6284077037357321 1102 4 0.6977362686699919 1102 5 0.7912598837977001 1102 6 0.7409421357743398 1102 7 0.6942184253946166 1102 8 0.7435389129917738 1102 9 0.8004600104690525 1102 10 0.9223236492483764 1102 11 0.9440107409909899 1102 12 0.9709810417730083 1102 13 0.9700529813893155 1102 14 1.0874641104423475 1102 15 0.9886611679351418 1102 16 1.1004393242909813 1102 17 1.0189195285911268 1102 18 1.0396582368892808 1102 19 1.0490930110305996 1102 20 0.9929034353373396 1102 21 1.0524944282592372 1102 22 1.0272677137412884 1102 23 1.0577735632173528 1102 24 1.0549115006451757 1102 25 1.060855554868077 1103 1 0.530760272542576 1103 2 0.5753048454822007 1103 3 0.592288378977166 1103 4 0.6577536428285882 1103 5 0.6500859113909385 1103 6 0.3731822244270333 1103 7 0.459473292778398 1103 8 0.5030123814215273 1103 9 0.609686486041852 1103 10 0.5744239302566143 1103 11 0.5015381796785263 1103 12 0.5533283423788973 1103 13 0.664658812003303 1103 14 0.6835649403641142 1103 15 0.6253312172996814 1103 16 0.6062426627694677 1103 17 0.6728297899801987 1103 18 0.6554028418629798 1103 19 0.6720631340171295 1103 20 0.7049985918560893 1103 21 0.535663713508427 1103 22 0.6774810133230034 1103 23 0.7068412063569554 1103 24 0.7622420731233319 1103 25 0.7640507396955059 1104 1 0.4543612492013622 1104 2 0.5254223405677116 1104 3 0.5673374626867549 1104 4 0.6569152747197293 1104 5 0.5722058750353511 1104 6 0.5707233905214153 1104 7 0.5737530130410065 1104 8 0.5993683823561042 1104 9 0.5589246946363033 1104 10 0.7665592826378926 1104 11 0.7497018067230741 1104 12 0.7439372372645252 1104 13 0.7093016232735394 1104 14 0.7719717045580197 1104 15 0.7327942042070177 1104 16 0.7696370413544145 1104 17 0.8523490693273672 1104 18 0.7626677201904215 1104 19 0.832261658508834 1104 20 0.9158047757306989 1104 21 0.8941275187834137 1104 22 0.8380284755881817 1104 23 0.7515018773694919 1104 24 0.6442240396766863 1104 25 0.7120868408403638 1105 1 0.34797169729101185 1105 2 0.33615651692577586 1105 3 0.22786257568253987 1105 4 0.36473526016146707 1105 5 0.4177249785174837 1105 6 0.43694061327969536 1105 7 0.3965231449117397 1105 8 0.5463221514214993 1105 9 0.4929791696079491 1105 10 0.5975734515475466 1105 11 0.6485278355348782 1105 12 0.515111039170641 1105 13 0.5531143634424822 1105 14 0.6128711002210139 1105 15 0.6534034503939452 1105 16 0.6220332291220885 1105 17 0.6568739791846596 1105 18 0.5953493175360833 1105 19 0.6904582965668311 1105 20 0.6111368225202654 1105 21 0.6719608775090578 1105 22 0.5404305882284746 1105 23 0.5963336956282532 1105 24 0.595707797061614 1105 25 0.6026967641555814 1106 1 0.4659984748766739 1106 2 0.45075632063036153 1106 3 0.39664546632220876 1106 4 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1107 17 0.5116259763284106 1107 18 0.521081811845896 1107 19 0.5592755411778896 1107 20 0.5462442443080988 1107 21 0.6334530897641528 1107 22 0.5804718253418741 1107 23 0.5127298218388106 1107 24 0.5735991702931926 1107 25 0.5631589411538442 1108 1 0.2363851091854272 1108 2 0.2433898726574668 1108 3 0.2818324979911253 1108 4 0.35015662790947033 1108 5 0.2588668545342401 1108 6 0.25170052668218545 1108 7 0.3018963011738265 1108 8 0.3746357326701997 1108 9 0.39043445364999485 1108 10 0.3105303505075341 1108 11 0.32444846655089066 1108 12 0.33941332682019976 1108 13 0.34163447812490233 1108 14 0.37773468145196887 1108 15 0.37969748792308167 1108 16 0.4191037654864047 1108 17 0.42777979214383066 1108 18 0.4417072418509349 1108 19 0.43826114559516327 1108 20 0.4410030251753412 1108 21 0.5523840041137262 1108 22 0.41717271712303017 1108 23 0.45919718527797926 1108 24 0.44974842860297315 1108 25 0.5065242208488385 1109 1 0.1958460341223578 1109 2 0.19935199645319202 1109 3 0.16506693674001127 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2214 23 -0.8832649180667644 2214 24 -0.9966998071502005 2214 25 -1.0910376225385718 2215 1 -0.597530061735803 2215 2 -0.6317549923923274 2215 3 -0.6907330021814602 2215 4 -0.477715220875929 2215 5 -0.8267460832604812 2215 6 -0.8102090530736064 2215 7 -0.8026615945247002 2215 8 -0.8580873023359388 2215 9 -1.043120022118245 2215 10 -1.029970923036963 2215 11 -1.052757198290962 2215 12 -1.0396484559654908 2215 13 -1.0892752658971219 2215 14 -1.2935322323951937 2215 15 -1.3523348024498176 2215 16 -1.2329616022882703 2215 17 -1.352548810525569 2215 18 -1.408836744312211 2215 19 -1.2915718903947706 2215 20 -1.223110439210977 2215 21 -1.271160770776433 2215 22 -1.305196563573105 2215 23 -1.2562268372841245 2215 24 -1.3296021050245628 2215 25 -1.454787683381788 2216 1 -1.3051626052727947 2216 2 -1.1810797718737795 2216 3 -1.1854993311489324 2216 4 -1.1693539095258956 2216 5 -1.4385571930414969 2216 6 -1.523668087432462 2216 7 -1.6531439702064503 2216 8 -1.5864102273027392 2216 9 -1.7882368933155632 2216 10 -1.7385940497335 2216 11 -1.6513505033426128 2216 12 -1.8450339032650902 2216 13 -1.9418284284148655 2216 14 -2.104615525857902 2216 15 -2.0987485995348294 2216 16 -2.1396246627042004 2216 17 -2.2811661331042714 2216 18 -2.246763761323521 2216 19 -2.21778122412087 2216 20 -2.2977101974541725 2216 21 -2.467561369086603 2216 22 -2.2225985277925346 2216 23 -2.290691326407959 2216 24 -2.3327292438786316 2216 25 -2.514250510604363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78838.0 3 0.0 4 1305.0 5 10331.0 6 11235.0 7 8785.0 8 6878.0 9 6193.0 10 5300.0 11 4777.0 12 4518.0 13 4131.0 14 3941.0 15 4055.0 16 4064.0 17 4223.0 18 4745.0 19 5758.0 20 7293.0 21 9146.0 22 11911.0 23 13677.0 24 16046.0 25 18121.0 26 20275.0 27 21924.0 28 24776.0 29 29790.0 30 37670.0 31 47869.0 32 61336.0 33 79186.0 34 105955.0 35 142115.0 36 204855.0 37 335753.0 38 1267772.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.69767164890133 19.888476120239353 14.464932732961078 26.94891949789824 2 13.479943117039891 20.79436474597765 39.68969674614836 26.0359953908341 3 16.531029521369422 25.16354189370234 30.257193633297312 28.048234951630924 4 11.268778757770981 17.866586804079088 37.83539619616324 33.02923824198668 5 12.822630585120177 38.12650047435581 35.82724754661957 13.223621393904454 6 31.193036162503812 37.887751661560735 18.16837250765889 12.750839668276557 7 27.387153770281376 31.908511050233322 22.51691797810534 18.187417201379965 8 25.099877601352325 36.54373198506828 20.58438876127386 17.77200165230553 9 25.919897089799516 15.324086351900453 20.05342886667703 38.702587691622995 10 14.376933259260783 29.510823965670575 34.48286767457155 21.629375100497093 11 34.01630833816274 22.772310802588027 24.355707861204237 18.855672998044994 12 22.461933430797774 25.16891486416513 31.64172712471905 20.72742458031805 13 28.255504664233484 21.834777582569487 26.093455365821228 23.816262387375804 14 20.98012275062602 21.754210693966257 27.879282516951747 29.386384038455972 15 23.212615358002733 29.876203398148327 23.608721809897098 23.30245943395184 16 22.447188029019866 28.498251317274942 26.770600793203553 22.28395986050164 17 21.858041365349205 28.392565334569092 27.40625876513701 22.343134534944696 18 21.721979698101297 28.056204107487098 28.599005867076453 21.62281032733515 19 23.1732561847816 27.354701592312885 27.51579605928185 21.956246163623668 20 23.253993927332985 27.397947150498737 27.730766490369575 21.617292431798706 21 23.270640107966162 26.839170196827922 27.21672408655887 22.67346560864705 22 22.939337271541266 27.4344038431057 27.25490599509858 22.37135289025446 23 22.636618481813226 27.160954836654525 27.252861313050907 22.949565368481338 24 22.646117596674777 27.25178859437457 27.654600965423747 22.447492843526902 25 22.840743780429165 27.091701472792547 27.399565180614644 22.667989566163644 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 127.0 1 127.0 2 280.0 3 433.0 4 433.0 5 433.0 6 1069.0 7 1705.0 8 1705.0 9 1705.0 10 2821.0 11 3937.0 12 3937.0 13 3937.0 14 7493.5 15 11050.0 16 11050.0 17 11050.0 18 20742.0 19 30434.0 20 30434.0 21 30434.0 22 48705.0 23 66976.0 24 66976.0 25 66976.0 26 95003.0 27 123030.0 28 123030.0 29 123030.0 30 156625.0 31 190220.0 32 190220.0 33 190220.0 34 218876.5 35 247533.0 36 247533.0 37 247533.0 38 271671.5 39 295810.0 40 295810.0 41 295810.0 42 311455.0 43 327100.0 44 327100.0 45 327100.0 46 337652.5 47 348205.0 48 348205.0 49 348205.0 50 336597.0 51 324989.0 52 324989.0 53 324989.0 54 290379.5 55 255770.0 56 255770.0 57 255770.0 58 217995.5 59 180221.0 60 180221.0 61 180221.0 62 146087.5 63 111954.0 64 111954.0 65 111954.0 66 85980.5 67 60007.0 68 60007.0 69 60007.0 70 43903.0 71 27799.0 72 27799.0 73 27799.0 74 19415.5 75 11032.0 76 11032.0 77 11032.0 78 7606.0 79 4180.0 80 4180.0 81 4180.0 82 2798.5 83 1417.0 84 1417.0 85 1417.0 86 953.5 87 490.0 88 490.0 89 490.0 90 301.5 91 113.0 92 113.0 93 113.0 94 63.5 95 14.0 96 14.0 97 14.0 98 7.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1843746749439046 2 0.008039482623096481 3 0.005448559313283397 4 0.0014859707218045628 5 0.07784200473453133 6 0.32893295490612284 7 0.027090389312898568 8 0.012764107482167398 9 0.004686523045691313 10 0.0035434686443031886 11 0.0 12 0.0 13 0.0 14 0.0014859707218045628 15 0.0 16 0.0 17 0.01962243389049615 18 0.028690665474841944 19 0.0 20 0.0 21 8.001380809716876E-4 22 1.1430544013881253E-4 23 0.005334253873144584 24 0.0 25 3.4291632041643754E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2624547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.72797054571495 #Duplication Level Percentage of deduplicated Percentage of total 1 81.01790644908223 56.49214194557095 2 12.447500440244161 17.358778881302374 3 3.4023703675844814 7.117211423296322 4 1.2600746993250038 3.5144980607973797 5 0.6062407566899897 2.113596881304577 6 0.34412851758504237 1.4397229882866232 7 0.20886931950571921 1.019482363087882 8 0.13966740480272333 0.7790979750624591 9 0.10306948218288713 0.6468143236029401 >10 0.4356031810496299 5.116698438782183 >50 0.020040883715607458 0.9669746537506148 >100 0.012232794629068934 1.6227452933053605 >500 0.0014211498497735354 0.7121801997847912 >1k 8.745537536613954E-4 1.100056572065585 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4024 0.1533216970395272 No Hit TATCAACGCAGAGTACTTTTTTTTT 2834 0.10798053911779823 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.8101813379604175E-5 7 0.0 0.0 0.0 0.0 3.8101813379604175E-5 8 0.0 0.0 0.0 0.0 3.8101813379604175E-5 9 0.0 0.0 0.0 3.8101813379604175E-5 7.620362675920835E-5 10 0.0 0.0 0.0 3.8101813379604175E-5 7.620362675920835E-5 11 0.0 0.0 0.0 1.9050906689802087E-4 7.620362675920835E-5 12 3.8101813379604175E-5 0.0 0.0 1.9050906689802087E-4 7.620362675920835E-5 13 3.8101813379604175E-5 0.0 0.0 1.9050906689802087E-4 7.620362675920835E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCGCG 50 0.0015103044 13.287953 7 GATCGAT 50 0.0015122575 13.285674 11 TAGGACC 490 0.0 11.462933 4 ACCGCAT 60 0.005927339 11.070763 8 AAGGCGT 105 3.3866472E-6 10.878823 6 GGCGAGG 510 0.0 10.419938 19 GTCCTAT 275 0.0 10.391283 1 GAACAGT 520 0.0 10.251199 6 GCGTGCG 75 0.0026753508 10.121069 9 GATATAC 665 0.0 9.883439 1 TTAGGAC 780 0.0 9.7651 3 TGTAGGA 1200 0.0 9.760303 2 CTAGCAC 235 0.0 9.723545 3 TTAAGGT 265 0.0 9.699686 4 GTAGGAC 1080 0.0 9.60913 3 TAAAGCG 90 0.0010927595 9.520062 4 TGGACCG 100 2.6986666E-4 9.520062 5 TAAGGTG 220 1.6370905E-11 9.520062 5 ACGAAAT 100 2.7832558E-4 9.490853 12 CGAAATC 120 1.720761E-5 9.490852 13 >>END_MODULE