FastQCFastQC Report
Thu 26 May 2016
SRR1513190_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513190_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057365
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCC400.00531740714.234799
CTAGGAC2150.012.4059463
TAGGACC3950.011.334454
GTCCTAA4600.011.2108721
TAGACCG600.00583206411.0947055
TAGGACA3750.010.92287354
CGCAGAT1408.521965E-910.8434468
GCACCGT909.647292E-510.5414716
TGGCGAG8400.010.28118218
TAGGACT2800.010.2061734
TCCTATA2250.010.1675132
GACGTGG4150.010.0595837
ATTAACC1909.458745E-1110.0273623
GTATCAA20400.09.8776991
GTCCTAC8250.09.8393961
GGCGAGG4150.09.83334619
TTAGGAC6200.09.8332843
TTAGACT1553.8973667E-89.8330444
TATGGCG9150.09.74962116
TCCTACA8550.09.5877862
Produced by FastQC (version 0.11.5)