##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513190_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2057365 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.627952259321997 31.0 30.0 34.0 16.0 34.0 2 28.512311135846094 31.0 28.0 34.0 16.0 34.0 3 28.682421932909328 31.0 29.0 34.0 16.0 34.0 4 31.733222349947628 35.0 32.0 37.0 17.0 37.0 5 31.837797862800233 35.0 32.0 37.0 17.0 37.0 6 32.20189854498351 35.0 33.0 37.0 19.0 37.0 7 32.14569315605155 36.0 33.0 37.0 19.0 37.0 8 32.03538798414477 36.0 33.0 37.0 17.0 37.0 9 33.16474227956634 38.0 33.0 39.0 17.0 39.0 10 32.74222853018303 38.0 32.0 39.0 11.0 39.0 11 33.164883236567164 38.0 33.0 39.0 17.0 39.0 12 33.138691967638216 38.0 33.0 39.0 17.0 39.0 13 33.17572331598914 38.0 33.0 39.0 17.0 39.0 14 33.33636034442114 39.0 32.0 41.0 10.0 41.0 15 33.72662118778146 39.0 33.0 41.0 11.0 41.0 16 33.54824642200096 39.0 33.0 41.0 10.0 41.0 17 33.16500669545754 38.0 32.0 41.0 10.0 41.0 18 32.867774556289234 38.0 32.0 41.0 8.0 41.0 19 33.49856588403127 39.0 32.0 41.0 10.0 41.0 20 33.69604129554065 39.0 33.0 41.0 8.0 41.0 21 33.7365226880014 39.0 33.0 41.0 2.0 41.0 22 33.41407966014781 39.0 33.0 41.0 2.0 41.0 23 32.9268574122725 39.0 32.0 41.0 2.0 41.0 24 33.145285838925034 39.0 33.0 41.0 2.0 41.0 25 32.75570499157904 39.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0112031054957882 1101 2 1.1575605958482527 1101 3 1.1352881015806027 1101 4 1.6121033001093217 1101 5 1.3653839336104276 1101 6 1.1757462105060554 1101 7 1.1650445388859865 1101 8 1.1785005784099383 1101 9 1.5269703907485734 1101 10 1.96799708641975 1101 11 1.51790116703679 1101 12 1.4470888451753083 1101 13 1.444302906709673 1101 14 2.3323627818084844 1101 15 2.0113168837272823 1101 16 1.9812931637081093 1101 17 2.409176383812081 1101 18 2.412234053768813 1101 19 1.9140229555244446 1101 20 1.5310223958284084 1101 21 1.5866783129825137 1101 22 1.7368424755368181 1101 23 2.0071264255658505 1101 24 1.832230624333043 1101 25 2.144907969906555 1102 1 1.1930922672623048 1102 2 1.3938220808349904 1102 3 1.371460152588753 1102 4 1.652585486524675 1102 5 1.6531032340453713 1102 6 1.34559506292252 1102 7 1.3431907846555475 1102 8 1.4118400681287753 1102 9 1.7981978575456878 1102 10 1.9472694099727477 1102 11 1.48578792115741 1102 12 1.437089086439066 1102 13 1.4623881210683578 1102 14 2.5313833341527356 1102 15 2.1072674795655644 1102 16 2.1781660526502407 1102 17 2.6571535120079943 1102 18 2.849827647467336 1102 19 2.192516413116927 1102 20 1.9529766904896633 1102 21 1.768613855626299 1102 22 1.9652373061696622 1102 23 2.2350293693164502 1102 24 2.1406391238055917 1102 25 2.4908313195792715 1103 1 0.8329520112584703 1103 2 1.1036891173441745 1103 3 0.9680767928380973 1103 4 1.3398324130134789 1103 5 1.2954679063151708 1103 6 1.0018163424089153 1103 7 0.988188586911896 1103 8 0.9347432208919386 1103 9 1.3783039486255149 1103 10 1.6027606056784904 1103 11 1.1404499681225246 1103 12 1.0955988497487326 1103 13 0.9855043883672678 1103 14 1.9324116117876997 1103 15 1.6017681255690022 1103 16 1.7091442483693058 1103 17 2.0146181816138693 1103 18 2.385100600568819 1103 19 1.6183433974130494 1103 20 1.3663248197436246 1103 21 1.3818833988952122 1103 22 1.820174570578203 1103 23 1.9987411252470295 1103 24 1.7078576128235383 1103 25 1.992846750403622 1104 1 0.7196185658229695 1104 2 0.9544592592327064 1104 3 0.7599262407212528 1104 4 1.1395666078383009 1104 5 1.026451824302324 1104 6 0.846038965829301 1104 7 0.8139779228788129 1104 8 0.8590139689524321 1104 9 1.229427212340667 1104 10 1.3448826258798476 1104 11 0.8590885665938899 1104 12 0.6804547219063295 1104 13 0.7444975113094401 1104 14 1.8676149869925567 1104 15 1.373204999198144 1104 16 1.4698937525222817 1104 17 1.8720517036616329 1104 18 1.9458629828000227 1104 19 1.298052262616487 1104 20 1.2436543574616294 1104 21 1.0880241095354535 1104 22 1.2970547289867653 1104 23 1.6187890120118098 1104 24 1.5582957545742673 1104 25 1.8424654526542525 1105 1 0.9752685147901339 1105 2 1.2093414005584329 1105 3 1.1501539055512744 1105 4 1.5252250723438046 1105 5 1.3682870737995394 1105 6 1.1248165532544903 1105 7 1.2112654320133913 1105 8 1.277567172948899 1105 9 1.5191467632517046 1105 10 1.8422071765996435 1105 11 1.374553428422736 1105 12 1.3431242525854188 1105 13 1.2666586462313987 1105 14 1.9718407474019486 1105 15 1.6628754297461796 1105 16 1.91629880628291 1105 17 2.242054461649225 1105 18 1.8908777692371643 1105 19 1.7475667964058559 1105 20 1.5102299635646332 1105 21 1.4974069041217462 1105 22 1.8156357141721458 1105 23 2.01932249136415 1105 24 1.7115261230373804 1105 25 1.987812204167625 1106 1 0.5982321916070674 1106 2 0.6987929614665589 1106 3 0.6565100368352503 1106 4 1.05950644753376 1106 5 1.0604067492660896 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1.5619277440221992 1107 19 1.070411948988351 1107 20 0.7451376020773779 1107 21 0.6467544115756425 1107 22 1.0161661623497338 1107 23 1.2668693651507041 1107 24 1.0700986708696334 1107 25 1.4064191780472797 1108 1 0.8589476924280142 1108 2 1.0498341225193677 1108 3 0.7769486251457813 1108 4 1.3952216550531027 1108 5 1.3601075020868088 1108 6 0.8913974255129986 1108 7 0.9127054348578412 1108 8 0.8772845057238783 1108 9 1.2251540837599961 1108 10 1.437827118564961 1108 11 1.0965252249102377 1108 12 0.8385962436412058 1108 13 0.9337329515633641 1108 14 1.927516709231007 1108 15 1.4520274083179885 1108 16 1.601548949986892 1108 17 1.851131615381874 1108 18 2.1368966255707065 1108 19 1.514896904712245 1108 20 1.371521541424542 1108 21 1.2676022032729577 1108 22 1.5032234427404987 1108 23 1.9325497767513795 1108 24 1.5911387247412847 1108 25 1.895872467002711 1109 1 0.630277255792759 1109 2 0.8713644299262491 1109 3 0.7550937928460542 1109 4 1.1120432497226709 1109 5 1.1147843245344724 1109 6 0.7499398551071934 1109 7 0.7646705164446388 1109 8 0.8383234894220237 1109 9 0.9776509286568711 1109 10 1.2731747421083313 1109 11 0.8585920646309972 1109 12 0.7652121314028903 1109 13 0.8982649397532896 1109 14 1.6461666158864787 1109 15 1.3388440207356211 1109 16 1.498565802405615 1109 17 1.7962281683226635 1109 18 1.8535590595849527 1109 19 1.3068952530084914 1109 20 1.171039019048024 1109 21 1.1475646328283702 1109 22 1.3792233341778086 1109 23 1.6757063532270067 1109 24 1.3891442730701016 1109 25 1.668883782724329 1110 1 0.25666908341160877 1110 2 0.4427042583436922 1110 3 0.48239120038472194 1110 4 0.8083373453989147 1110 5 0.7462065148724655 1110 6 0.4681340828949274 1110 7 0.3990335489146446 1110 8 0.3139152448237468 1110 9 0.6517074968337582 1110 10 0.9751280436062615 1110 11 0.5530404203061039 1110 12 0.6094279307156825 1110 13 0.28743552216154455 1110 14 1.1713478512770834 1110 15 0.8492524582950267 1110 16 1.0793775382628539 1110 17 1.3249232066426373 1110 18 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2215 19 -3.7621947314557502 2215 20 -3.4970823089055365 2215 21 -3.628789157785345 2215 22 -9.440231945559013 2215 23 -10.75040042997649 2215 24 -7.222184164822252 2215 25 -5.701504760304509 2216 1 -2.67174704364097 2216 2 -2.5997250830637455 2216 3 -2.5529591471956046 2216 4 -7.468485015881257 2216 5 -4.153174962514324 2216 6 -3.530265401551322 2216 7 -3.0251348425533457 2216 8 -8.449912836343483 2216 9 -4.892130970668742 2216 10 -3.2208732687198456 2216 11 -3.1950243253649084 2216 12 -3.4137185243228068 2216 13 -3.032580093326498 2216 14 -2.573206802468391 2216 15 -3.2233179211557825 2216 16 -2.721272476475626 2216 17 -2.5727894862671903 2216 18 -8.129359018290021 2216 19 -5.842806549177158 2216 20 -5.41730996266395 2216 21 -4.318913207891388 2216 22 -4.1006731502757745 2216 23 -3.28589942730229 2216 24 -3.696845639477111 2216 25 -3.0687275191843177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 126629.0 3 3.0 4 1058.0 5 6051.0 6 6968.0 7 6280.0 8 5881.0 9 5596.0 10 5190.0 11 4980.0 12 4743.0 13 4654.0 14 4758.0 15 4765.0 16 4913.0 17 5089.0 18 5892.0 19 7228.0 20 9153.0 21 11619.0 22 14193.0 23 16690.0 24 19570.0 25 22318.0 26 24898.0 27 27564.0 28 30970.0 29 36341.0 30 43679.0 31 52383.0 32 65641.0 33 84029.0 34 108626.0 35 141106.0 36 195932.0 37 275046.0 38 666929.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.17004799679015 17.92626793184329 12.049302739133038 25.854381332233515 2 15.098813189254823 19.75138222497901 40.31141539740399 24.83838918836217 3 18.773739501019136 24.171008706168873 29.355045723588518 27.70020606922347 4 11.803275488016478 16.063166988391078 38.247948244263256 33.88560927932919 5 13.173177699373934 37.525318697660275 35.84372352103967 13.457780081926115 6 32.7634017380888 36.47259175137046 17.807874876274447 12.95613163426629 7 28.668360421746957 31.11876315042334 21.98702167462012 18.22585475320958 8 25.94934893522896 35.67314774077972 20.308894198632483 18.068609125358833 9 26.777514879773186 14.738693049591465 19.475038004615488 39.00875406601986 10 14.275622944140917 28.26411839803668 35.15308766576921 22.30717099205319 11 35.31547132313119 22.024799776757284 23.776078609439004 18.88365029067252 12 23.199130292728842 24.362499902760813 31.23390691487293 21.204462889637416 13 29.18528364742753 21.123640737567907 25.453395396958594 24.237680218045966 14 21.23813177094956 20.64513964753019 28.08381914736902 30.032909434151232 15 23.86811164810841 29.14285797645219 23.065387403920358 23.923642971519044 16 23.24742263029293 27.25191084629513 26.447658708898935 23.053007814513 17 22.486157191671413 27.394828938532434 27.3665281940089 22.752485675787256 18 22.56552704613392 26.865116487124084 28.3542923934754 22.2150640732666 19 23.905210541950623 26.65655716543717 26.896977894420047 22.541254398192155 20 24.220506789099126 26.580829102959857 26.870022798070554 22.328641309870463 21 23.97448470789133 26.236659731436916 26.408978877776423 23.379876682895333 22 23.864019307161556 26.671882803234137 26.522012080105462 22.942085809498845 23 23.385421731266653 26.478976642875203 26.82135980863105 23.314241817227092 24 23.550419820711806 26.450017847121654 26.816151304687814 23.18341102747873 25 23.548675548372135 26.296140158427534 26.847733648029475 23.307450645170857 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 135.0 1 135.0 2 229.0 3 323.0 4 323.0 5 323.0 6 663.0 7 1003.0 8 1003.0 9 1003.0 10 1643.0 11 2283.0 12 2283.0 13 2283.0 14 4495.5 15 6708.0 16 6708.0 17 6708.0 18 12920.5 19 19133.0 20 19133.0 21 19133.0 22 30684.0 23 42235.0 24 42235.0 25 42235.0 26 60684.0 27 79133.0 28 79133.0 29 79133.0 30 103145.5 31 127158.0 32 127158.0 33 127158.0 34 150678.0 35 174198.0 36 174198.0 37 174198.0 38 196010.0 39 217822.0 40 217822.0 41 217822.0 42 234758.0 43 251694.0 44 251694.0 45 251694.0 46 263544.5 47 275395.0 48 275395.0 49 275395.0 50 270780.0 51 266165.0 52 266165.0 53 266165.0 54 243547.0 55 220929.0 56 220929.0 57 220929.0 58 191994.5 59 163060.0 60 163060.0 61 163060.0 62 134326.0 63 105592.0 64 105592.0 65 105592.0 66 81997.5 67 58403.0 68 58403.0 69 58403.0 70 43150.5 71 27898.0 72 27898.0 73 27898.0 74 19677.0 75 11456.0 76 11456.0 77 11456.0 78 7975.0 79 4494.0 80 4494.0 81 4494.0 82 2991.0 83 1488.0 84 1488.0 85 1488.0 86 998.0 87 508.0 88 508.0 89 508.0 90 320.0 91 132.0 92 132.0 93 132.0 94 73.0 95 14.0 96 14.0 97 14.0 98 10.0 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17916120863337326 2 0.2458484517817694 3 0.24993134421942628 4 0.41363588862452705 5 0.24439027591117765 6 0.019102103904751953 7 0.019296527354164185 8 0.01701205182357044 9 0.018373015969456074 10 0.02133797357299264 11 0.019977009427107003 12 0.02824000602712693 13 0.02678183015653518 14 0.02469177807535367 15 0.04218988852245469 16 0.020560279775343707 17 0.042870370595397514 18 0.03193405156595937 19 0.026927647743594352 20 0.02974678776007174 21 0.03480179744478982 22 0.04500902853893208 23 0.029406546723600335 24 0.04914052683894205 25 0.037815360910679434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2057365.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.98105539202349 #Duplication Level Percentage of deduplicated Percentage of total 1 83.22003411532567 61.56705953611993 2 11.202422285976242 16.575340473272934 3 2.983290909300436 6.62121030034427 4 1.0936597070955354 3.236403974826359 5 0.503215823011667 1.8614218838184413 6 0.2787577940699166 1.2373677482426866 7 0.1715769864557789 0.8885412577287009 8 0.10999457873182103 0.6510012017584912 9 0.07916301045964799 0.5270906755633097 >10 0.33402647074870595 4.104036864990701 >50 0.015358984717305742 0.7819741907270502 >100 0.007238588615544449 1.113184836436873 >500 8.626153364074238E-4 0.4327787487335642 >1k 3.9813015526477143E-4 0.4025883074367819 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.86058623530584E-5 7 0.0 0.0 0.0 0.0 4.86058623530584E-5 8 0.0 0.0 0.0 4.86058623530584E-5 4.86058623530584E-5 9 0.0 0.0 0.0 4.86058623530584E-5 4.86058623530584E-5 10 0.0 0.0 0.0 9.72117247061168E-5 4.86058623530584E-5 11 0.0 0.0 0.0 9.72117247061168E-5 4.86058623530584E-5 12 0.0 0.0 0.0 9.72117247061168E-5 9.72117247061168E-5 13 0.0 0.0 0.0 9.72117247061168E-5 1.4581758705917521E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGATCCC 40 0.005317407 14.23479 9 CTAGGAC 215 0.0 12.405946 3 TAGGACC 395 0.0 11.33445 4 GTCCTAA 460 0.0 11.210872 1 TAGACCG 60 0.005832064 11.094705 5 TAGGACA 375 0.0 10.9228735 4 CGCAGAT 140 8.521965E-9 10.843446 8 GCACCGT 90 9.647292E-5 10.541471 6 TGGCGAG 840 0.0 10.281182 18 TAGGACT 280 0.0 10.206173 4 TCCTATA 225 0.0 10.167513 2 GACGTGG 415 0.0 10.059583 7 ATTAACC 190 9.458745E-11 10.027362 3 GTATCAA 2040 0.0 9.877699 1 GTCCTAC 825 0.0 9.839396 1 GGCGAGG 415 0.0 9.833346 19 TTAGGAC 620 0.0 9.833284 3 TTAGACT 155 3.8973667E-8 9.833044 4 TATGGCG 915 0.0 9.749621 16 TCCTACA 855 0.0 9.587786 2 >>END_MODULE