FastQCFastQC Report
Thu 26 May 2016
SRR1513190_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513190_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2057365
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22410.10892573753320388No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGACA602.5130095E-514.2833684
GGACGTT550.003017380112.1183366
TAAGACG657.8694057E-411.7196874
TAGGACT2300.011.1782884
TACACCG600.005775315711.1092865
GTGCCGA600.005931257311.06959411
GTCCTAA5600.010.7185391
TAGGACC4000.010.7125264
GATATAC2700.010.2333381
CGTGCGC1303.913683E-710.21709310
ACCGTAT750.0026750410.1210198
TGTAGGA9350.010.0843462
GTAGGAC8500.010.0831163
GTTATAC1252.4769543E-69.9086951
AGGACGT8300.09.8664245
AGGACTG4350.09.8505995
TTAGGAC5950.09.7630183
ATAGGAC2054.0017767E-119.755213
TATACTG2750.09.6953775
GTATTAG1803.97813E-99.5275921
Produced by FastQC (version 0.11.5)