FastQCFastQC Report
Thu 26 May 2016
SRR1513189_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513189_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392286
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCCG550.003042048312.1053035
CGTTCGA550.003101280712.0741728
TATACGT657.946485E-411.7062255
GGTCGGT752.0974912E-411.386611
GTCCTAC8900.011.3788791
GTCTACG600.00563453411.1462911
ACGTTCG600.00593720811.0682227
GGACCGA951.3823063E-510.9845436
TAGGACC4550.010.8926714
GTCTAAC803.5798602E-410.748211
GTCCTAA5250.010.73683551
AGGACGT7900.010.5948755
GGACGTG7950.010.5009476
TTAGGAC6400.010.4244073
TAGGACG8150.010.408184
ACCGTCC1201.5518472E-610.2774218
GTAGGAC9500.010.1328373
AATCGAA750.002678605710.1195177
TAAGACT2105.456968E-129.9849484
GGCGAGG5800.09.97942619
Produced by FastQC (version 0.11.5)