##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513189_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2392286 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.130336840996435 31.0 30.0 34.0 23.0 34.0 2 29.000437656701582 31.0 30.0 34.0 16.0 34.0 3 29.174935187515207 31.0 30.0 34.0 22.0 34.0 4 32.218717996092444 35.0 33.0 37.0 19.0 37.0 5 32.35511305922452 35.0 33.0 37.0 20.0 37.0 6 32.725564585505246 37.0 35.0 37.0 25.0 37.0 7 32.679710954292254 37.0 35.0 37.0 25.0 37.0 8 32.56115238729817 37.0 35.0 37.0 23.0 37.0 9 33.773015851783605 39.0 34.0 39.0 19.0 39.0 10 33.35376539427142 38.0 33.0 39.0 17.0 39.0 11 33.79508720947245 39.0 34.0 39.0 22.0 39.0 12 33.783938876873414 39.0 34.0 39.0 22.0 39.0 13 33.82168394581584 39.0 35.0 39.0 22.0 39.0 14 34.03170941935872 39.0 33.0 41.0 11.0 41.0 15 34.446554885160054 39.0 34.0 41.0 17.0 41.0 16 34.282523912274705 39.0 34.0 41.0 17.0 41.0 17 33.88887532677949 39.0 33.0 41.0 11.0 41.0 18 33.6154907899808 39.0 33.0 41.0 10.0 41.0 19 34.2472614060359 39.0 34.0 41.0 18.0 41.0 20 34.4506894242578 39.0 34.0 41.0 18.0 41.0 21 34.49483631973769 40.0 34.0 41.0 18.0 41.0 22 34.17786418513506 39.0 34.0 41.0 10.0 41.0 23 33.71604314868707 39.0 33.0 41.0 2.0 41.0 24 33.95531010924279 39.0 34.0 41.0 2.0 41.0 25 33.59712258484144 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7931858890312213 1101 2 1.0303248146807853 1101 3 1.013424774404406 1101 4 1.3345839593262099 1101 5 1.2523569595927455 1101 6 0.9185037238842568 1101 7 0.9118053206995356 1101 8 0.9429674318201151 1101 9 1.2776478587924203 1101 10 1.5496535942988032 1101 11 1.2520360273040083 1101 12 1.1010782098076959 1101 13 1.0926680041938326 1101 14 2.0242097463127564 1101 15 1.6518714650582496 1101 16 1.7558026910853854 1101 17 2.107571085082597 1101 18 2.113004976215187 1101 19 1.6722801798455862 1101 20 1.3362328886796675 1101 21 1.3045960044680882 1101 22 1.5634297981066467 1101 23 1.8875555545605707 1101 24 1.5223697052925118 1101 25 1.6837546896706428 1102 1 0.9101015206591505 1102 2 1.1163261292325615 1102 3 1.0439782594431897 1102 4 1.2492529112624453 1102 5 1.2905766730604782 1102 6 0.9535783407543832 1102 7 0.89372385947825 1102 8 1.0401102423950803 1102 9 1.4157506730079774 1102 10 1.571657006065763 1102 11 1.2306026688340026 1102 12 1.1389362036816166 1102 13 1.0836459122684872 1102 14 2.0624941707138476 1102 15 1.609064456460736 1102 16 1.7131500366219186 1102 17 2.0834773165195557 1102 18 2.387176728714749 1102 19 1.7497490108804925 1102 20 1.5333874956866183 1102 21 1.4763425331944973 1102 22 1.7168892752648972 1102 23 2.002695831242576 1102 24 1.6691625846369575 1102 25 2.1037272754727354 1103 1 0.6942025119732911 1103 2 0.9558462804381591 1103 3 0.8060277729879637 1103 4 1.181487242145053 1103 5 1.0709652481330423 1103 6 0.8891936150539053 1103 7 0.8692603710709434 1103 8 0.8617264149660997 1103 9 1.236621408848272 1103 10 1.494267509776634 1103 11 1.036415169861172 1103 12 1.0301565881726304 1103 13 0.9904455195188362 1103 14 1.873057729298118 1103 15 1.3845312753958297 1103 16 1.4446082209082647 1103 17 1.792608126493846 1103 18 2.0941168010480737 1103 19 1.4707602038642946 1103 20 1.2503571020501383 1103 21 1.2632787882311973 1103 22 1.4359972981696316 1103 23 1.8265183822082065 1103 24 1.5477981964417111 1103 25 1.736845293057236 1104 1 0.8045757950162873 1104 2 1.0568548753230331 1104 3 0.8561196955797641 1104 4 1.345022885294071 1104 5 1.2198963703065218 1104 6 1.0260012644808896 1104 7 0.9758709707806688 1104 8 0.9673327996925494 1104 9 1.2887414472459398 1104 10 1.4265889382633574 1104 11 1.0044251275753595 1104 12 0.9325884297093694 1104 13 0.9567395677701782 1104 14 1.8631685597827996 1104 15 1.5847017120720182 1104 16 1.6389926810806514 1104 17 2.0941265608988147 1104 18 2.1264141479819756 1104 19 1.4974443195107696 1104 20 1.3141334500439115 1104 21 1.2711595277799503 1104 22 1.4760280175660938 1104 23 1.780056085650287 1104 24 1.5927129804754045 1104 25 1.936859033614951 1105 1 0.6953474162289481 1105 2 0.8332052269570447 1105 3 0.7800591417665927 1105 4 1.0821418883092377 1105 5 0.9073503147777373 1105 6 0.621560338790843 1105 7 0.5293644135401863 1105 8 0.6322810535555661 1105 9 0.8406599223119784 1105 10 1.180904254043675 1105 11 0.7057429138224762 1105 12 0.7196577080497306 1105 13 0.660763148200914 1105 14 1.382067544564805 1105 15 1.156855752980384 1105 16 1.3521863418631384 1105 17 1.600486440153901 1105 18 1.2256488005971917 1105 19 1.0722439107941852 1105 20 1.0029351928470902 1105 21 0.8569655373822513 1105 22 1.1091761691715263 1105 23 1.36649920490526 1105 24 1.1980039400290607 1105 25 1.3687512116310074 1106 1 0.5489542883971374 1106 2 0.7417847547951126 1106 3 0.7163021688972755 1106 4 1.2301816685124152 1106 5 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1107 18 1.9320065624380618 1107 19 1.3927003003117306 1107 20 1.2329186359983169 1107 21 1.050522929729631 1107 22 1.3368430909178102 1107 23 1.598730173134797 1107 24 1.2988961639085232 1107 25 1.6444190745114042 1108 1 0.6516471534892752 1108 2 0.8141075831011513 1108 3 0.7026924432467467 1108 4 1.0559990869491571 1108 5 0.9802417447429761 1108 6 0.6166466676487801 1108 7 0.48995882073370467 1108 8 0.6463020875215406 1108 9 0.757104721094322 1108 10 1.013569178772613 1108 11 0.7669783744259462 1108 12 0.5072668615395344 1108 13 0.4981216748784192 1108 14 1.477807303838091 1108 15 0.8794000456790556 1108 16 1.0237246179787363 1108 17 1.3986974798691492 1108 18 1.6723006621516276 1108 19 1.0328732603870776 1108 20 0.8527255912794942 1108 21 0.8660887488106326 1108 22 1.1183828499167845 1108 23 1.5269593819658667 1108 24 1.1907508639281374 1108 25 1.4569539783747203 1109 1 0.3641665328113106 1109 2 0.5613205682294442 1109 3 0.45588962810509415 1109 4 0.781251333425395 1109 5 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1.3181592684722077 1110 18 1.0842890372115264 1110 19 0.7229369932050744 1110 20 0.5453943851232168 1110 21 0.36755662833581937 1110 22 0.6602694090610015 1110 23 -0.4561806630756351 1110 24 0.3158741158779179 1110 25 0.7600215679855609 1111 1 0.29714769383496886 1111 2 0.6392540772195723 1111 3 0.5227499609179453 1111 4 0.9292556585326253 1111 5 0.7626390629690434 1111 6 0.522934632208397 1111 7 0.5321898226297392 1111 8 0.5613052452002023 1111 9 0.6311776402269942 1111 10 1.0157839881782778 1111 11 0.7321366982171043 1111 12 0.48252832663091993 1111 13 0.3718856922181146 1111 14 1.281407558168901 1111 15 0.8114430101349654 1111 16 0.8446940360575326 1111 17 1.3779284653152146 1111 18 1.6354452952780036 1111 19 1.138829507763127 1111 20 0.8224882611522375 1111 21 0.5609886149937182 1111 22 0.9169043553892564 1111 23 1.2307827121689385 1111 24 0.912197974777321 1111 25 1.1604734848184606 1112 1 0.1770318654080114 1112 2 0.33880458593732143 1112 3 0.12784151261644183 1112 4 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 2 128682.0 3 1.0 4 1115.0 5 6200.0 6 7155.0 7 6396.0 8 5854.0 9 5672.0 10 5177.0 11 4919.0 12 4867.0 13 4883.0 14 4958.0 15 4839.0 16 5074.0 17 5243.0 18 6003.0 19 7293.0 20 9313.0 21 11773.0 22 14617.0 23 17326.0 24 19914.0 25 22918.0 26 25600.0 27 28999.0 28 32224.0 29 38832.0 30 46077.0 31 55858.0 32 70241.0 33 90688.0 34 119063.0 35 158110.0 36 224940.0 37 322036.0 38 869426.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.46375765351628 17.70352218776459 12.039773579027502 25.792946579691623 2 15.116423320036512 19.593769409824 40.28572099140247 25.004086278737013 3 18.90437357031434 24.03129235620579 29.201379371621456 27.86295470185841 4 11.807240452592335 15.984450485691331 38.105297729268614 34.10301133244772 5 13.301287021243605 37.391466516912324 35.65757104600336 13.649675415840711 6 33.01264590748298 36.12748506276128 17.67663485992589 13.183234169829849 7 28.821912630768292 30.989168944563694 21.804387108426784 18.384531316241233 8 25.967896963912203 35.45640821101227 20.299590987937844 18.276103837137693 9 26.71819459646582 14.827149953884913 19.472899965800416 38.98175548384885 10 14.27091359953106 28.127282556387893 34.94454550813808 22.657258335942963 11 35.32406327321926 22.034161919168355 23.625734074871247 19.01604073274114 12 23.471592303636484 24.231494679554427 30.818067897825035 21.478845118984054 13 29.136434508500685 21.08427261563343 25.405549601924378 24.373743273941507 14 21.225735264296265 20.520878324164784 28.028997056894056 30.22438935464489 15 24.000869808851142 28.909518970614677 23.001216896036933 24.088394324497248 16 23.41736322251991 27.173873051232395 26.20889651504661 23.199867211201088 17 22.43802586019437 27.253943428733923 27.37354264590268 22.934488065169027 18 22.388135840561247 26.826407597785355 28.25289489320475 22.532561668448643 19 23.937459050220163 26.470021311655685 26.86819846892249 22.724321169201666 20 24.303987268704837 26.405562498876268 26.71748926549609 22.5729609669228 21 24.12665239086265 26.046986379444515 26.34918683531685 23.477174394375986 22 23.957361034442382 26.42741841053177 26.457486492363795 23.15773406266205 23 23.265781087907957 26.26383969432773 26.88079559086646 23.589583626897856 24 23.709449227553552 26.32620279035066 26.642826165779166 23.321521816316622 25 23.640908684150883 26.180452234535718 26.763496408401053 23.415142672912346 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 193.0 1 193.0 2 306.5 3 420.0 4 420.0 5 420.0 6 824.5 7 1229.0 8 1229.0 9 1229.0 10 2000.5 11 2772.0 12 2772.0 13 2772.0 14 5304.5 15 7837.0 16 7837.0 17 7837.0 18 14883.5 19 21930.0 20 21930.0 21 21930.0 22 34727.5 23 47525.0 24 47525.0 25 47525.0 26 68592.5 27 89660.0 28 89660.0 29 89660.0 30 115846.0 31 142032.0 32 142032.0 33 142032.0 34 169455.0 35 196878.0 36 196878.0 37 196878.0 38 222204.5 39 247531.0 40 247531.0 41 247531.0 42 268336.5 43 289142.0 44 289142.0 45 289142.0 46 305571.0 47 322000.0 48 322000.0 49 322000.0 50 317898.5 51 313797.0 52 313797.0 53 313797.0 54 288149.0 55 262501.0 56 262501.0 57 262501.0 58 228663.0 59 194825.0 60 194825.0 61 194825.0 62 160787.0 63 126749.0 64 126749.0 65 126749.0 66 98499.5 67 70250.0 68 70250.0 69 70250.0 70 51738.0 71 33226.0 72 33226.0 73 33226.0 74 23443.5 75 13661.0 76 13661.0 77 13661.0 78 9516.5 79 5372.0 80 5372.0 81 5372.0 82 3617.5 83 1863.0 84 1863.0 85 1863.0 86 1263.5 87 664.0 88 664.0 89 664.0 90 431.5 91 199.0 92 199.0 93 199.0 94 110.0 95 21.0 96 21.0 97 21.0 98 15.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17422666019029498 2 0.26133999028544247 3 0.2621760107278143 4 0.47013609576781373 5 0.2573270921620575 6 0.018601454842773816 7 0.01922847017455271 8 0.017765434400401958 9 0.018559653820655223 10 0.021109516169889386 11 0.020900511059296422 12 0.027714077664627055 13 0.027379669487678317 14 0.024871608160562744 15 0.03996177714537476 16 0.021652929457431094 17 0.040379787366560685 18 0.031225363522588857 19 0.026627251089543644 20 0.029218914460896396 21 0.034569445292076284 22 0.043723869136048116 23 0.029469720593607956 24 0.046691741706468205 25 0.03645049128741296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2392286.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.72596427314078 #Duplication Level Percentage of deduplicated Percentage of total 1 81.53471721111565 57.66621496494001 2 12.00558322580442 16.982129006129227 3 3.2922728843280242 6.985475231832419 4 1.283338259032373 3.630613434347132 5 0.6066151545731778 2.145172087494417 6 0.33248214547539734 1.4109072205410085 7 0.209795760246648 1.0386605210702586 8 0.152132441346511 0.8607770889167217 9 0.10613620576431164 0.675592694727608 >10 0.44534867438000547 5.37633733586844 >50 0.020210226088701853 0.9729512060829915 >100 0.009815399352341587 1.2629523723608187 >500 0.0010150389373946105 0.4661679285512336 >1k 5.373735550832649E-4 0.5260489071377444 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 4.180102211859284E-5 0.0 6 0.0 0.0 0.0 4.180102211859284E-5 4.180102211859284E-5 7 0.0 0.0 0.0 4.180102211859284E-5 4.180102211859284E-5 8 0.0 0.0 0.0 4.180102211859284E-5 8.360204423718568E-5 9 0.0 0.0 0.0 8.360204423718568E-5 8.360204423718568E-5 10 0.0 0.0 0.0 8.360204423718568E-5 8.360204423718568E-5 11 0.0 0.0 0.0 8.360204423718568E-5 8.360204423718568E-5 12 0.0 0.0 0.0 8.360204423718568E-5 2.090051105929642E-4 13 0.0 0.0 0.0 8.360204423718568E-5 2.090051105929642E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCCG 55 0.0030420483 12.105303 5 CGTTCGA 55 0.0031012807 12.074172 8 TATACGT 65 7.946485E-4 11.706225 5 GGTCGGT 75 2.0974912E-4 11.3866 11 GTCCTAC 890 0.0 11.378879 1 GTCTACG 60 0.005634534 11.146291 1 ACGTTCG 60 0.005937208 11.068222 7 GGACCGA 95 1.3823063E-5 10.984543 6 TAGGACC 455 0.0 10.892671 4 GTCTAAC 80 3.5798602E-4 10.74821 1 GTCCTAA 525 0.0 10.7368355 1 AGGACGT 790 0.0 10.594875 5 GGACGTG 795 0.0 10.500947 6 TTAGGAC 640 0.0 10.424407 3 TAGGACG 815 0.0 10.40818 4 ACCGTCC 120 1.5518472E-6 10.277421 8 GTAGGAC 950 0.0 10.132837 3 AATCGAA 75 0.0026786057 10.119517 7 TAAGACT 210 5.456968E-12 9.984948 4 GGCGAGG 580 0.0 9.979426 19 >>END_MODULE