FastQCFastQC Report
Thu 26 May 2016
SRR1513189_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513189_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2392286
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30370.12694970417416646No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG604.1378618E-412.65047559
TAGGACC5050.011.4999994
GTTCGCA951.38036285E-510.9859389
CTAGACG700.001465332510.880564
TACACCG909.3133734E-510.5783225
TGCGTTG5650.010.41471912
ACCGTGC1553.7362042E-910.4073418
ATTATAC2250.010.1558293
TGAACCG856.466335E-410.0805195
GAACCGT1151.0016642E-59.9338016
TAGACAG3650.09.9117435
ACCTAAT1356.2069375E-79.879111
GTAGGAC9650.09.8664153
TTAGGAC6350.09.7459973
GGCGAGG4200.09.71396119
CTCCTAA3450.09.6643471
CTGCGTT6000.09.64780111
CGTTGAT5850.09.5719514
GTCCTAG2800.09.5262841
TAGGACT3700.09.5204914
Produced by FastQC (version 0.11.5)