##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513189_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2392286 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.587933466149114 31.0 31.0 34.0 27.0 34.0 2 30.692950174017653 31.0 31.0 34.0 27.0 34.0 3 30.802329654564712 33.0 31.0 34.0 27.0 34.0 4 34.18781700850149 37.0 35.0 37.0 30.0 37.0 5 33.91469372809104 37.0 35.0 37.0 30.0 37.0 6 33.8268998773558 37.0 35.0 37.0 30.0 37.0 7 33.68806238050133 37.0 35.0 37.0 28.0 37.0 8 33.654745711842146 37.0 35.0 37.0 28.0 37.0 9 35.1975344921134 39.0 35.0 39.0 28.0 39.0 10 35.039668333970106 39.0 35.0 39.0 28.0 39.0 11 35.05009476291714 39.0 35.0 39.0 28.0 39.0 12 34.97502932341702 39.0 35.0 39.0 27.0 39.0 13 34.94689974359253 39.0 35.0 39.0 27.0 39.0 14 36.10670588717235 40.0 36.0 41.0 27.0 41.0 15 36.07758395108277 40.0 36.0 41.0 27.0 41.0 16 36.03966791595988 40.0 36.0 41.0 27.0 41.0 17 35.96572566992408 40.0 36.0 41.0 27.0 41.0 18 35.91716458650847 40.0 36.0 41.0 27.0 41.0 19 35.989519647734426 40.0 36.0 41.0 27.0 41.0 20 35.95087836487778 40.0 36.0 41.0 27.0 41.0 21 35.891347857237804 40.0 36.0 41.0 27.0 41.0 22 35.83445290404241 40.0 36.0 41.0 27.0 41.0 23 35.7535712703247 40.0 36.0 41.0 27.0 41.0 24 35.691075398175634 40.0 36.0 41.0 27.0 41.0 25 35.63277133252462 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44738761261725557 1101 2 0.44790782141322083 1101 3 0.4758446745494638 1101 4 0.6031198536816973 1101 5 0.4829213532176979 1101 6 0.4900903301409585 1101 7 0.5626280821836289 1101 8 0.4707026221279236 1101 9 0.6224528789307158 1101 10 0.700790770213537 1101 11 0.7380062668903378 1101 12 0.6555422567051963 1101 13 0.6541325638262734 1101 14 0.7529431565743678 1101 15 0.7579401890323822 1101 16 0.6732695866799432 1101 17 0.7842679252696172 1101 18 0.8362489994108984 1101 19 0.7291031078601478 1101 20 0.8057830041510456 1101 21 0.7108706426762694 1101 22 0.7905077954988897 1101 23 0.8068580304424771 1101 24 0.8628333656695801 1101 25 0.7676442125085217 1102 1 0.48944302228184 1102 2 0.609802715688307 1102 3 0.49597292161419304 1102 4 0.6067736814947793 1102 5 0.5632935427500527 1102 6 0.5181522021846874 1102 7 0.5072993466078373 1102 8 0.5644932127063669 1102 9 0.588970134883759 1102 10 0.6639173991821892 1102 11 0.6994533163219074 1102 12 0.6226379573979273 1102 13 0.7318319174753967 1102 14 0.8290261892546766 1102 15 0.7543497004745205 1102 16 0.8683210076658767 1102 17 0.9111692123716963 1102 18 0.8819106315884682 1102 19 0.8970013532256118 1102 20 0.8249939212889359 1102 21 0.7594119920751226 1102 22 0.7938793803632436 1102 23 0.809592995276688 1102 24 0.9028520753593838 1102 25 1.0276983308591667 1103 1 0.364966521085087 1103 2 0.45736935978835547 1103 3 0.42819486692538433 1103 4 0.5091826036793279 1103 5 0.4877049561811404 1103 6 0.3092676348369139 1103 7 0.38683410326033396 1103 8 0.4486854999728678 1103 9 0.4896852507829337 1103 10 0.5016063118965377 1103 11 0.5118493837124376 1103 12 0.5193625374615607 1103 13 0.6000538043521004 1103 14 0.7418612219386915 1103 15 0.5992311368133585 1103 16 0.7343280209162941 1103 17 0.6877475569463911 1103 18 0.7080395982562706 1103 19 0.596526676201222 1103 20 0.7454584659631749 1103 21 0.6260829831058246 1103 22 0.6463443591676423 1103 23 0.5933582264529775 1103 24 0.7032584332542839 1103 25 0.692584386380986 1104 1 0.3950398425352333 1104 2 0.4052774666067229 1104 3 0.3013565534436502 1104 4 0.49809473257555936 1104 5 0.42499448965521225 1104 6 0.48254017872012867 1104 7 0.41344682809199185 1104 8 0.4868708893484879 1104 9 0.35197908905198005 1104 10 0.514789973820001 1104 11 0.47667284442606217 1104 12 0.5710290900188753 1104 13 0.5243196134646055 1104 14 0.5582284714695902 1104 15 0.4606523943257983 1104 16 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-0.8978423501336366 2215 22 -0.9268431646356774 2215 23 -0.8710495407149708 2215 24 -0.8649732511381458 2215 25 -1.0861040025308455 2216 1 -0.459899775779963 2216 2 -0.4972136745889273 2216 3 -0.5220691448038153 2216 4 -0.2949762655991677 2216 5 -0.5250007698918893 2216 6 -0.6987744431470944 2216 7 -0.7141532837667626 2216 8 -0.7395015381590966 2216 9 -0.919430960655049 2216 10 -0.8626393091128008 2216 11 -0.7885362414154358 2216 12 -0.9740762529362925 2216 13 -1.0417029447174855 2216 14 -1.1716210848797886 2216 15 -1.199281855459688 2216 16 -1.0176847361919883 2216 17 -1.246832350740668 2216 18 -1.0061037423244912 2216 19 -1.0944353326561398 2216 20 -1.2036824964501065 2216 21 -1.3501802932279503 2216 22 -1.3038190760332995 2216 23 -1.3427677884450802 2216 24 -1.2384325731090158 2216 25 -1.3530909488730032 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 67038.0 3 1.0 4 1112.0 5 8668.0 6 9618.0 7 7463.0 8 5879.0 9 5121.0 10 4668.0 11 4093.0 12 3839.0 13 3743.0 14 3598.0 15 3577.0 16 3713.0 17 3818.0 18 4277.0 19 5017.0 20 6421.0 21 8267.0 22 10591.0 23 12602.0 24 14852.0 25 16230.0 26 17991.0 27 20028.0 28 22448.0 29 26914.0 30 34309.0 31 44287.0 32 56535.0 33 73562.0 34 97065.0 35 130599.0 36 187186.0 37 305082.0 38 1162074.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.58325833487937 18.540246006096755 12.981468087930995 27.89502757109288 2 14.458719799805944 19.93742681575316 38.558718169425006 27.045135215015893 3 17.21443053320235 24.002424597119745 29.548021654160483 29.235123215517422 4 11.667934655385748 16.470671838945925 36.61296525432231 35.24842825134602 5 13.335561707056986 37.54716294418091 35.42838114226883 13.688894206493268 6 33.13652325589686 36.46747579961608 17.280170846469268 13.115830098017794 7 28.491476453100635 31.086924707623737 21.800168087606256 18.621430751669376 8 25.99541876800061 35.829891556327034 19.861760341949378 18.312929333722977 9 26.471384952896287 14.64725721604837 19.082439026021582 39.798918805033765 10 14.718569477578905 28.458788164313102 34.17844493056359 22.644197427544395 11 35.358314181498365 21.990054700817545 23.51052507935924 19.14110603832485 12 23.210696050010764 24.30446205197123 30.837839137059337 21.647002760958664 13 29.02194804467359 20.97876257270243 25.470533205477942 24.528756177146043 14 21.706612357916068 20.58540753299825 26.954479635890593 30.75350047319509 15 23.940573994915322 28.991809507726085 22.82879220962711 24.238824287731482 16 23.570175137922472 27.31738596472161 25.656296947772965 23.45614194958295 17 22.687314366370543 27.534567992079634 26.611626016804024 23.1664916247458 18 22.71953162794996 26.97688537771528 27.56235044026956 22.741232554065203 19 23.93066715267322 26.51593496764183 26.70955730209515 22.843840577589802 20 24.169894402257924 26.416323131933222 26.71616186358989 22.697620602218965 21 23.934963754030697 25.971735584588 26.40952134921451 23.68377931216679 22 23.87236470571023 26.48547309371584 26.275381068727178 23.366781131846746 23 23.43589514207088 26.31063005638022 26.41505490247062 23.83841989907828 24 23.48653129266317 26.349901307786777 26.768831151459317 23.394736248090737 25 23.576713428194022 26.262561238650996 26.54133295433645 23.61939237881853 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 145.0 1 145.0 2 227.5 3 310.0 4 310.0 5 310.0 6 826.5 7 1343.0 8 1343.0 9 1343.0 10 2028.5 11 2714.0 12 2714.0 13 2714.0 14 5020.5 15 7327.0 16 7327.0 17 7327.0 18 14133.0 19 20939.0 20 20939.0 21 20939.0 22 33630.5 23 46322.0 24 46322.0 25 46322.0 26 66983.5 27 87645.0 28 87645.0 29 87645.0 30 113919.5 31 140194.0 32 140194.0 33 140194.0 34 166207.5 35 192221.0 36 192221.0 37 192221.0 38 218149.5 39 244078.0 40 244078.0 41 244078.0 42 264844.0 43 285610.0 44 285610.0 45 285610.0 46 302890.5 47 320171.0 48 320171.0 49 320171.0 50 318275.0 51 316379.0 52 316379.0 53 316379.0 54 292175.5 55 267972.0 56 267972.0 57 267972.0 58 233638.0 59 199304.0 60 199304.0 61 199304.0 62 164930.5 63 130557.0 64 130557.0 65 130557.0 66 101390.0 67 72223.0 68 72223.0 69 72223.0 70 53265.0 71 34307.0 72 34307.0 73 34307.0 74 24175.5 75 14044.0 76 14044.0 77 14044.0 78 9838.5 79 5633.0 80 5633.0 81 5633.0 82 3816.5 83 2000.0 84 2000.0 85 2000.0 86 1322.0 87 644.0 88 644.0 89 644.0 90 412.0 91 180.0 92 180.0 93 180.0 94 101.0 95 22.0 96 22.0 97 22.0 98 12.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1863907576268055 2 0.00865281157854872 3 0.005684939008128626 4 0.001922847017455271 5 0.07954734509168218 6 0.3311894982456111 7 0.028257490952168764 8 0.014755760807863273 9 0.004932520609993956 10 0.004054699145503505 11 0.0 12 4.180102211859284E-5 13 0.0 14 0.001588438840506528 15 0.0 16 0.0 17 0.01998088857268738 18 0.028550098106998913 19 0.0 20 0.0 21 8.360204423718568E-4 22 4.180102211859284E-5 23 0.005643137986010034 24 0.0 25 2.508061327115571E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2392286.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.74778900508984 #Duplication Level Percentage of deduplicated Percentage of total 1 79.65133876978247 53.96202092948156 2 12.983755556154359 17.592414638240168 3 3.7251733995630842 7.571167844429191 4 1.4557833089405356 3.945044018049397 5 0.7010454817411635 2.3747140689985957 6 0.3884789757534427 1.5791155009354592 7 0.2446528590196585 1.1602283190649072 8 0.1691848652275341 0.9169520441831632 9 0.11679468098966377 0.7121323264144066 >10 0.523160486127292 6.100436930603006 >50 0.02627326776434434 1.2182166355866335 >100 0.012443465107011107 1.5348199371241178 >500 0.0011736384761055199 0.5217622541642678 >1k 7.412453533230085E-4 0.8109745527250787 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3037 0.12694970417416646 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.180102211859284E-5 2 0.0 0.0 0.0 0.0 4.180102211859284E-5 3 0.0 0.0 0.0 0.0 4.180102211859284E-5 4 0.0 0.0 0.0 4.180102211859284E-5 4.180102211859284E-5 5 0.0 0.0 0.0 4.180102211859284E-5 8.360204423718568E-5 6 0.0 0.0 0.0 4.180102211859284E-5 8.360204423718568E-5 7 0.0 4.180102211859284E-5 0.0 4.180102211859284E-5 8.360204423718568E-5 8 0.0 4.180102211859284E-5 0.0 4.180102211859284E-5 8.360204423718568E-5 9 0.0 4.180102211859284E-5 0.0 1.6720408847437136E-4 8.360204423718568E-5 10 0.0 4.180102211859284E-5 0.0 1.6720408847437136E-4 8.360204423718568E-5 11 0.0 4.180102211859284E-5 0.0 2.090051105929642E-4 8.360204423718568E-5 12 0.0 4.180102211859284E-5 0.0 2.090051105929642E-4 8.360204423718568E-5 13 0.0 4.180102211859284E-5 0.0 2.090051105929642E-4 2.090051105929642E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTGCG 60 4.1378618E-4 12.6504755 9 TAGGACC 505 0.0 11.499999 4 GTTCGCA 95 1.38036285E-5 10.985938 9 CTAGACG 70 0.0014653325 10.88056 4 TACACCG 90 9.3133734E-5 10.578322 5 TGCGTTG 565 0.0 10.414719 12 ACCGTGC 155 3.7362042E-9 10.407341 8 ATTATAC 225 0.0 10.155829 3 TGAACCG 85 6.466335E-4 10.080519 5 GAACCGT 115 1.0016642E-5 9.933801 6 TAGACAG 365 0.0 9.911743 5 ACCTAAT 135 6.2069375E-7 9.87911 1 GTAGGAC 965 0.0 9.866415 3 TTAGGAC 635 0.0 9.745997 3 GGCGAGG 420 0.0 9.713961 19 CTCCTAA 345 0.0 9.664347 1 CTGCGTT 600 0.0 9.647801 11 CGTTGAT 585 0.0 9.57195 14 GTCCTAG 280 0.0 9.526284 1 TAGGACT 370 0.0 9.520491 4 >>END_MODULE