FastQCFastQC Report
Thu 26 May 2016
SRR1513188_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513188_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121306
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT32860.15490457293761484No Hit
GTCCTAAAGTGTGTATTTCTCATTT28640.1350111676486089No Hit
CTGTAGGACGTGGAATATGGCAAGA26980.12718579969132224No Hit
CTTTAGGACGTGAAATATGGCGAGG23310.10988513679780286No Hit
GTATCAACGCAGAGTACTTTTTTTT21410.10092839034066749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7100.013.9580384
GGCGAGG7550.011.56390219
GTCCTAA9300.011.19496251
TGTAGGA19900.011.116612
GTAGGAC18350.011.0090333
TGGCGAG17550.010.81474818
CTGTAGG20650.010.7311681
TTAGGGC1605.16593E-1010.7201763
AAATGTC6100.010.5757087
AATGTCC6050.010.5063018
TTATACT2550.010.4632654
AGGACGT17300.010.3882045
GTCCTAC17000.010.3382731
GACGTGG8100.010.30697
AGGACCT13900.010.2612725
GTCTAGG2053.6379788E-1210.2505671
GGACGTG16500.010.1765196
GGACCTG13300.010.1285346
GCCGGTT750.002672670610.12212711
TAGAAAT7250.010.1205034
Produced by FastQC (version 0.11.5)