##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513188_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2121306 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.567522083094094 31.0 30.0 34.0 25.0 34.0 2 29.449770094460675 31.0 30.0 34.0 23.0 34.0 3 29.627749603310413 31.0 30.0 34.0 25.0 34.0 4 32.69830990908431 37.0 33.0 37.0 24.0 37.0 5 32.853626492358956 37.0 35.0 37.0 25.0 37.0 6 33.23152435339362 37.0 35.0 37.0 27.0 37.0 7 33.199355962788964 37.0 35.0 37.0 27.0 37.0 8 33.08690872509671 37.0 35.0 37.0 26.0 37.0 9 34.335023329967484 39.0 35.0 39.0 25.0 39.0 10 33.922334637247054 39.0 34.0 39.0 22.0 39.0 11 34.36629416029559 39.0 35.0 39.0 27.0 39.0 12 34.36161213893705 39.0 35.0 39.0 27.0 39.0 13 34.39971885244279 39.0 35.0 39.0 27.0 39.0 14 34.64188193499665 40.0 34.0 41.0 17.0 41.0 15 35.07767149105315 40.0 34.0 41.0 24.0 41.0 16 34.919623100109085 40.0 34.0 41.0 22.0 41.0 17 34.541208576226154 39.0 34.0 41.0 17.0 41.0 18 34.275827721224566 39.0 33.0 41.0 17.0 41.0 19 34.909825833708105 40.0 34.0 41.0 22.0 41.0 20 35.12337352555454 40.0 35.0 41.0 25.0 41.0 21 35.16618488798881 40.0 36.0 41.0 25.0 41.0 22 34.8600984487858 40.0 35.0 41.0 19.0 41.0 23 34.39584812375018 40.0 34.0 41.0 16.0 41.0 24 34.65145905399787 40.0 34.0 41.0 18.0 41.0 25 34.29559761769401 39.0 34.0 41.0 15.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8200886945854222 1101 2 1.0278416429048391 1101 3 1.010395783613049 1101 4 1.3528156496866544 1101 5 1.2028281214052967 1101 6 1.0039446812386146 1101 7 1.0263696346512177 1101 8 1.0626738150474182 1101 9 1.3293285715892438 1101 10 1.6707256065269647 1101 11 1.2223995048090543 1101 12 1.1887143679820937 1101 13 1.0499579049261385 1101 14 2.0149003716536384 1101 15 1.6256699375745 1101 16 1.7126142559640343 1101 17 2.023175699873697 1101 18 2.122757468542112 1101 19 1.6579877703116708 1101 20 1.2680628955179074 1101 21 1.3510257782680668 1101 22 1.5014330884809794 1101 23 1.8730323294375921 1101 24 1.5802256958689682 1101 25 1.8370968282367883 1102 1 1.0885752313183126 1102 2 1.3149781173179633 1102 3 1.1654279451099079 1102 4 1.5511006424019627 1102 5 1.53605945370942 1102 6 1.2475711755460281 1102 7 1.1825852329388624 1102 8 1.260875408123617 1102 9 1.6536692570709093 1102 10 1.7564886442361711 1102 11 1.4099989187630655 1102 12 1.4313418041126411 1102 13 1.3482909853010128 1102 14 2.304241621820367 1102 15 1.892761983282817 1102 16 2.0026645052686547 1102 17 2.3036014672960192 1102 18 2.6301591354106364 1102 19 1.967891732969889 1102 20 1.8503399611792801 1102 21 1.638087336093605 1102 22 1.8343818227432607 1102 23 2.030430546872509 1102 24 1.8860406232801097 1102 25 2.120608280100832 1103 1 0.5952866393593652 1103 2 0.9937083198856378 1103 3 0.7413514046949956 1103 4 1.1697844966042723 1103 5 1.0270257822013917 1103 6 0.7362992240190138 1103 7 0.714863544395115 1103 8 0.7339885917685649 1103 9 1.122172940532451 1103 10 1.3527397321347436 1103 11 0.9790179698833441 1103 12 0.9682448579405758 1103 13 0.997039803526711 1103 14 1.8049229779188636 1103 15 1.4105159024935645 1103 16 1.4788069476567145 1103 17 1.8472803299340228 1103 18 2.073335449978714 1103 19 1.5297736041937924 1103 20 1.188607725848776 1103 21 1.234504069230347 1103 22 1.3967051117773437 1103 23 1.773343585444131 1103 24 1.4303674303323746 1103 25 1.7629890937591242 1104 1 0.7256773957215792 1104 2 0.8744125821857054 1104 3 0.7694999057594245 1104 4 1.0976746291562094 1104 5 0.9605374108855003 1104 6 0.822480790730296 1104 7 0.6994083569848968 1104 8 0.7609256902813684 1104 9 1.023271346879767 1104 10 1.179556490790496 1104 11 0.8141596693646633 1104 12 0.6159907467456733 1104 13 0.66252031353239 1104 14 1.649246644510626 1104 15 1.395756961396259 1104 16 1.4339117167688755 1104 17 1.8305176921980149 1104 18 1.8746135056836195 1104 19 1.2479585293755733 1104 20 1.0055236598343384 1104 21 0.9834706051853317 1104 22 1.305093143984564 1104 23 1.6802869990980795 1104 24 1.4519941874813824 1104 25 1.586126022067269 1105 1 0.5191674814614373 1105 2 0.7453875698837429 1105 3 0.6376990434221881 1105 4 1.0779459724391387 1105 5 0.9592770672715432 1105 6 0.6989655298523587 1105 7 0.6629806680221151 1105 8 0.6835210163492889 1105 9 0.9465446939791349 1105 10 1.1930939041238986 1105 11 0.7599271104317751 1105 12 0.7074400853751754 1105 13 0.6022025232799848 1105 14 1.4454955231528928 1105 15 1.0605768628655383 1105 16 1.2651961446577573 1105 17 1.4838530856098728 1105 18 1.3251478203706668 1105 19 1.0677368302828398 1105 20 0.9130909576612041 1105 21 0.8142714831913267 1105 22 1.144643240287948 1105 23 1.3951517350427594 1105 24 1.1595521237190454 1105 25 1.3227750967475629 1106 1 0.4453146550248306 1106 2 0.5798778064075272 1106 3 0.5539392267659018 1106 4 0.9786605586019235 1106 5 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1.6177111718191313 1107 18 1.770697281812545 1107 19 1.2575753012664705 1107 20 0.9105347467621812 1107 21 0.7943052996312971 1107 22 1.0947580363827285 1107 23 1.4500598443802772 1107 24 1.2615033883970383 1107 25 1.5014868371454853 1108 1 0.6384809403297425 1108 2 0.8803585381714107 1108 3 0.6362805031275123 1108 4 1.1604906818927176 1108 5 0.9725647550277401 1108 6 0.6632727284144693 1108 7 0.5997078362095039 1108 8 0.6729887111268908 1108 9 0.8507388871742236 1108 10 1.0849017857511214 1108 11 0.7662224851271873 1108 12 0.5755067818179569 1108 13 0.522219356162374 1108 14 1.5014785376647026 1108 15 0.8967354588304985 1108 16 1.0832933044556867 1108 17 1.3469523861280805 1108 18 1.5250831941257843 1108 19 0.956530747323086 1108 20 0.7292599277377505 1108 21 0.7130508679972607 1108 22 0.9659595732321833 1108 23 1.1752891650454416 1108 24 0.9114996781918023 1108 25 1.2434767288974413 1109 1 0.30777303600048356 1109 2 0.5911247823249184 1109 3 0.46539399469728693 1109 4 0.7093276940752276 1109 5 0.8148614636669151 1109 6 0.5002906986038056 1109 7 0.39417589605188397 1109 8 0.4064870991643872 1109 9 0.5812779032385862 1109 10 0.8951412762559627 1109 11 0.44122515492841785 1109 12 0.27187607619136145 1109 13 0.34667451134316707 1109 14 1.305654218800079 1109 15 0.7715544537754297 1109 16 0.9832631338348534 1109 17 1.2766204868949629 1109 18 1.3660175573427153 1109 19 0.6983818792067851 1109 20 0.7062662180134751 1109 21 0.5220952957300327 1109 22 0.9005341338725188 1109 23 1.2398169537555077 1109 24 0.9332008680643256 1109 25 1.1272343303629526 1110 1 0.15616171862378891 1110 2 0.37744325964467507 1110 3 0.3114845689308865 1110 4 0.6729233678788447 1110 5 0.5388837403497746 1110 6 0.23304938254786833 1110 7 0.13363336050164776 1110 8 0.16114458415918165 1110 9 0.33117737934031766 1110 10 0.7276446148233902 1110 11 0.2374041461674281 1110 12 0.22710662078141297 1110 13 0.1309491250232213 1110 14 0.855763180835794 1110 15 0.5617207217706692 1110 16 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2215 15 -4.655521089196512 2215 16 -9.544857578757629 2215 17 -4.621120217286094 2215 18 -2.9627057890030137 2215 19 -3.495918358677695 2215 20 -3.221382452853355 2215 21 -3.367308096507532 2215 22 -9.28116335792149 2215 23 -10.706394143845088 2215 24 -6.7062843500553875 2215 25 -4.92912567300716 2216 1 -1.598832859356822 2216 2 -1.5120629706880315 2216 3 -1.2992004682914455 2216 4 -6.907991456520904 2216 5 -3.2422981933485744 2216 6 -2.3549727778773786 2216 7 -1.7575543611192366 2216 8 -7.524778290109847 2216 9 -3.4083288509923904 2216 10 -1.6917969837076754 2216 11 -1.6961895882401095 2216 12 -1.7548778050500147 2216 13 -1.643511999430416 2216 14 -0.9752965079469433 2216 15 -1.6136303789325268 2216 16 -1.266007294344277 2216 17 -1.0817558181457585 2216 18 -7.044112107696552 2216 19 -4.30778728191509 2216 20 -3.615653522696803 2216 21 -2.671785683345597 2216 22 -2.375446122121602 2216 23 -1.6436539988947914 2216 24 -2.1048898766134485 2216 25 -1.5178356204249965 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 91360.0 3 2.0 4 751.0 5 4370.0 6 5352.0 7 4898.0 8 4578.0 9 4369.0 10 4094.0 11 3958.0 12 3716.0 13 3810.0 14 3766.0 15 3880.0 16 3901.0 17 4206.0 18 4781.0 19 5913.0 20 7545.0 21 9759.0 22 12059.0 23 14076.0 24 16672.0 25 19069.0 26 21742.0 27 24628.0 28 27447.0 29 33123.0 30 39965.0 31 47949.0 32 61319.0 33 79637.0 34 105457.0 35 140979.0 36 200789.0 37 294292.0 38 807094.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.632063442288185 18.419354960584702 12.249250514297373 25.69933108282974 2 14.319613037077644 19.976700937869953 41.86567358155735 23.83801244349505 3 18.792316788333586 24.540877911191814 29.524842075612767 27.141963224861833 4 11.368595924274397 16.471445674040456 39.26361890184995 32.8963394998352 5 12.62899606608077 38.01804007266692 36.39697611859604 12.955987742656275 6 31.97154271257037 37.204664173738635 18.129049809682815 12.694743304008188 7 28.075936750179046 31.370398642477827 22.361490728329127 18.192173879013996 8 24.85994510017662 37.05615491896571 20.686492964958223 17.39740701589945 9 26.517970469474626 14.956971584083165 20.16125990370235 38.36379804273986 10 13.816537371449373 28.68458363765214 35.857297309531006 21.641581681367487 11 34.52812992275416 22.23702208175715 24.73691481551857 18.497933179970115 12 23.290808567824683 24.600313572009572 31.85736010090889 20.251517759256856 13 29.016960597225776 21.77967208600398 25.650728354597145 23.55263896217309 14 20.233404690726996 21.68494610473505 28.68666245437999 29.39498675015796 15 23.08627530320458 30.706935800376623 23.197526165646355 23.00926273077244 16 22.078162116184178 27.8918502466436 28.006991457295438 22.022996179876785 17 21.4675256078927 28.311576842542113 28.359444266284356 21.86145328328083 18 21.386936521671473 27.25727314095476 30.030438852330306 21.325351485043463 19 23.43081752999169 26.833286651822892 27.616781578627226 22.119114239558193 20 23.653849327696815 27.112149554745024 27.919007683801773 21.314993433756385 21 23.111271655175244 26.761191700144348 26.97759608632974 23.149940558350668 22 23.163424548416717 27.85509907926577 27.14343183958671 21.838044532730798 23 22.10513211775069 27.61783462316875 27.88058003927035 22.396453219810212 24 22.976482218285614 26.922587144643977 28.00414853216669 22.09678210490372 25 22.649999693471557 27.146394683193424 27.911348201691943 22.29225742164308 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 164.0 2 274.5 3 385.0 4 385.0 5 385.0 6 852.5 7 1320.0 8 1320.0 9 1320.0 10 2095.0 11 2870.0 12 2870.0 13 2870.0 14 5613.0 15 8356.0 16 8356.0 17 8356.0 18 16021.0 19 23686.0 20 23686.0 21 23686.0 22 37617.5 23 51549.0 24 51549.0 25 51549.0 26 74882.5 27 98216.0 28 98216.0 29 98216.0 30 126214.0 31 154212.0 32 154212.0 33 154212.0 34 179562.5 35 204913.0 36 204913.0 37 204913.0 38 223394.0 39 241875.0 40 241875.0 41 241875.0 42 254260.0 43 266645.0 44 266645.0 45 266645.0 46 275304.0 47 283963.0 48 283963.0 49 283963.0 50 272616.0 51 261269.0 52 261269.0 53 261269.0 54 231963.0 55 202657.0 56 202657.0 57 202657.0 58 173610.5 59 144564.0 60 144564.0 61 144564.0 62 117332.0 63 90100.0 64 90100.0 65 90100.0 66 69358.0 67 48616.0 68 48616.0 69 48616.0 70 35277.0 71 21938.0 72 21938.0 73 21938.0 74 15397.0 75 8856.0 76 8856.0 77 8856.0 78 6139.0 79 3422.0 80 3422.0 81 3422.0 82 2314.5 83 1207.0 84 1207.0 85 1207.0 86 797.0 87 387.0 88 387.0 89 387.0 90 245.0 91 103.0 92 103.0 93 103.0 94 62.5 95 22.0 96 22.0 97 22.0 98 16.5 99 11.0 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1816805307673669 2 0.2517788569871579 3 0.25550297788249315 4 0.45641694314728753 5 0.25220312392460115 6 0.01758350751848154 7 0.01805491522675182 8 0.016593551331113943 9 0.018007774455924795 10 0.021166206101335686 11 0.01956341989321673 12 0.02738878785050342 13 0.02672881705892502 14 0.024796045455016864 15 0.041153892931995666 16 0.020364812997276206 17 0.04082390753620647 18 0.031160049516665678 19 0.026021705496519595 20 0.028803010975314264 21 0.03483702964117388 22 0.04398233918161736 23 0.029321559454411575 24 0.04893212011845533 25 0.03700550509921718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2121306.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.45845755057222 #Duplication Level Percentage of deduplicated Percentage of total 1 81.35516871777727 57.3215970162115 2 12.390257171746345 17.459968179523255 3 3.353649802351871 7.088789767154828 4 1.2450073407476157 3.5088518747286668 5 0.5586569867227732 1.9681054792168549 6 0.3077767621684358 1.3011285559378376 7 0.17421009854874647 0.8592202383434497 8 0.11533084153467893 0.6500826562034356 9 0.08419193780106612 0.533883066809115 >10 0.3810416924095845 4.655612852548651 >50 0.020213674113128435 0.9821836193152196 >100 0.01178757129508032 1.6373196824817002 >500 0.0018951819484212533 0.8719613275182543 >1k 8.1222083503736E-4 1.1612956840073503 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 3286 0.15490457293761484 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2864 0.1350111676486089 No Hit CTGTAGGACGTGGAATATGGCAAGA 2698 0.12718579969132224 No Hit CTTTAGGACGTGAAATATGGCGAGG 2331 0.10988513679780286 No Hit GTATCAACGCAGAGTACTTTTTTTT 2141 0.10092839034066749 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.714077082702826E-5 0.0 7 0.0 0.0 0.0 4.714077082702826E-5 0.0 8 0.0 0.0 0.0 4.714077082702826E-5 0.0 9 0.0 0.0 0.0 9.428154165405651E-5 0.0 10 0.0 0.0 0.0 9.428154165405651E-5 0.0 11 0.0 0.0 0.0 1.8856308330811303E-4 0.0 12 0.0 0.0 0.0 1.8856308330811303E-4 0.0 13 0.0 0.0 0.0 2.3570385413514128E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 710 0.0 13.958038 4 GGCGAGG 755 0.0 11.563902 19 GTCCTAA 930 0.0 11.1949625 1 TGTAGGA 1990 0.0 11.11661 2 GTAGGAC 1835 0.0 11.009033 3 TGGCGAG 1755 0.0 10.814748 18 CTGTAGG 2065 0.0 10.731168 1 TTAGGGC 160 5.16593E-10 10.720176 3 AAATGTC 610 0.0 10.575708 7 AATGTCC 605 0.0 10.506301 8 TTATACT 255 0.0 10.463265 4 AGGACGT 1730 0.0 10.388204 5 GTCCTAC 1700 0.0 10.338273 1 GACGTGG 810 0.0 10.3069 7 AGGACCT 1390 0.0 10.261272 5 GTCTAGG 205 3.6379788E-12 10.250567 1 GGACGTG 1650 0.0 10.176519 6 GGACCTG 1330 0.0 10.128534 6 GCCGGTT 75 0.0026726706 10.122127 11 TAGAAAT 725 0.0 10.120503 4 >>END_MODULE