FastQCFastQC Report
Thu 26 May 2016
SRR1513188_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513188_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2121306
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33090.1559888106666365No Hit
GTATCAACGCAGAGTACTTTTTTTT31450.14825772425100386No Hit
CTGTAGGACGTGGAATATGGCAAGA31080.1465135157304038No Hit
GTCCTAAAGTGTGTATTTCTCATTT29180.13755676927326843No Hit
CTTTAGGACGTGAAATATGGCGAGG27570.1299671051701169No Hit
GTACATGGGAAGCAGTGGTATCAAC24460.11530632544291113No Hit
CCCATGTACTCTGCGTTGATACCAC23240.10955515140201368No Hit
GTCCTACAGTGTGCATTTCTCATTT21790.10271973963209458No Hit
TATCAACGCAGAGTACTTTTTTTTT21620.10191834652803508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6950.012.1911984
TGCGCAT752.0999055E-411.38506510
CCGATCC600.00593343511.0690759
AAAGGCG1502.060915E-910.7894395
ACTAGAC1252.1724918E-710.6640193
TAACCTA1105.896827E-610.3855565
GGCGAGG6500.010.36332819
GTAGGAC16750.010.2888443
GGACGTG15150.010.2420146
AATACCG750.002602233610.1547665
AGGACGT15900.010.1188415
AGGCGTG1701.5861588E-910.0491987
TGTAGGA18050.010.0228112
ATACCGT951.6109228E-410.020446
CGTGCAC951.6663597E-49.98689910
GTCCTAC19000.09.9755291
GGACCTG11950.09.957556
TGTACCG1356.2609433E-79.8726895
CTGTAGG18150.09.8654671
TAGAGTG3100.09.8271935
Produced by FastQC (version 0.11.5)