##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513188_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2121306
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.81069492095907	31.0	31.0	34.0	27.0	34.0
2	30.90885049115969	33.0	31.0	34.0	27.0	34.0
3	31.008289233142225	33.0	31.0	34.0	27.0	34.0
4	34.402630737856775	37.0	35.0	37.0	31.0	37.0
5	34.1690001348226	37.0	35.0	37.0	30.0	37.0
6	34.097530012171745	37.0	35.0	37.0	30.0	37.0
7	33.96696799047379	37.0	35.0	37.0	30.0	37.0
8	33.93776098309249	37.0	35.0	37.0	30.0	37.0
9	35.49722340859829	39.0	37.0	39.0	30.0	39.0
10	35.33652806337228	39.0	35.0	39.0	29.0	39.0
11	35.36316401311268	39.0	35.0	39.0	30.0	39.0
12	35.30043331796544	39.0	35.0	39.0	29.0	39.0
13	35.279542885373445	39.0	35.0	39.0	29.0	39.0
14	36.44315671572135	40.0	37.0	41.0	29.0	41.0
15	36.4357263874236	40.0	37.0	41.0	29.0	41.0
16	36.4033562343198	40.0	37.0	41.0	29.0	41.0
17	36.33530806022328	40.0	37.0	41.0	28.0	41.0
18	36.28812297707167	40.0	36.0	41.0	28.0	41.0
19	36.36467251777914	40.0	37.0	41.0	28.0	41.0
20	36.33242634490262	40.0	37.0	41.0	28.0	41.0
21	36.276800235326725	40.0	37.0	41.0	28.0	41.0
22	36.22604942426977	40.0	37.0	41.0	28.0	41.0
23	36.14552073109679	40.0	37.0	41.0	28.0	41.0
24	36.095072092380825	40.0	37.0	41.0	28.0	41.0
25	36.03638371833201	40.0	37.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31567304100089544
1101	2	0.4257730812239373
1101	3	0.31994426844824275
1101	4	0.5107480861663163
1101	5	0.3793337610594847
1101	6	0.5246038287326087
1101	7	0.44068428036378293
1101	8	0.45451764889818236
1101	9	0.5744422953521919
1101	10	0.660664659828015
1101	11	0.6171708085826282
1101	12	0.5771454748068265
1101	13	0.6237820185356568
1101	14	0.6384209354168462
1101	15	0.7098495342606128
1101	16	0.5908862869339373
1101	17	0.6457631428627195
1101	18	0.7000899607242275
1101	19	0.5223305842183308
1101	20	0.6317390162479981
1101	21	0.6716129533437538
1101	22	0.6362710811742929
1101	23	0.5953543179912728
1101	24	0.6435730081991125
1101	25	0.6321859961878147
1102	1	0.5762942558862996
1102	2	0.6853489388681133
1102	3	0.5865003488287321
1102	4	0.7126036259311306
1102	5	0.6961945414082393
1102	6	0.8275828617976018
1102	7	0.6999194872761478
1102	8	0.6994235509046334
1102	9	0.6864862355149981
1102	10	0.8254607114274535
1102	11	0.7948743852109033
1102	12	0.8351063842921533
1102	13	0.8537205470737135
1102	14	0.9247801374580931
1102	15	0.8566399512741398
1102	16	0.9598637674982982
1102	17	1.0396771155217408
1102	18	0.9554784614879566
1102	19	0.964181770031395
1102	20	0.9973526091627036
1102	21	1.0345738040609547
1102	22	1.0597710758085057
1102	23	1.0534731348815427
1102	24	1.1297508977896413
1102	25	1.1595834419716624
1103	1	0.4161539712268265
1103	2	0.41594164599582584
1103	3	0.3947315140768666
1103	4	0.5047374252918502
1103	5	0.3973710364599796
1103	6	0.32627970517069116
1103	7	0.36899330933258057
1103	8	0.35175105324294265
1103	9	0.36007800368353315
1103	10	0.48547017571601003
1103	11	0.4682330791229248
1103	12	0.46106520373520965
1103	13	0.45743939875349326
1103	14	0.45268262863731223
1103	15	0.4292861384962592
1103	16	0.525552551892396
1103	17	0.5761454136880175
1103	18	0.4427224659075861
1103	19	0.5363704897219037
1103	20	0.4055463856258186
1103	21	0.5081982571338912
1103	22	0.39334788038770796
1103	23	0.4891167801911749
1103	24	0.4704998080741234
1103	25	0.49072533220892467
1104	1	0.35573671968591114
1104	2	0.40557378902376584
1104	3	0.3893139193652573
1104	4	0.5524686668113787
1104	5	0.5148293602302232
1104	6	0.43784369710027704
1104	7	0.4693019028269845
1104	8	0.5351966991071961
1104	9	0.4569606931332686
1104	10	0.563858065852564
1104	11	0.5671066588414817
1104	12	0.5795944601873728
1104	13	0.48387854958711785
1104	14	0.6585259150879921
1104	15	0.6719306298662673
1104	16	0.6710104026273527
1104	17	0.5698999071730739
1104	18	0.5872354466642165
1104	19	0.6223396507826138
1104	20	0.6360983621789558
1104	21	0.6227652427525356
1104	22	0.5238299821851413
1104	23	0.6576327000096924
1104	24	0.5806628031478738
1104	25	0.45666338979876997
1105	1	0.026316477748331124
1105	2	0.08311196477134075
1105	3	-0.022463416028948302
1105	4	0.0755584712275521
1105	5	-0.017581572281244462
1105	6	0.08207891178908966
1105	7	0.027522161583689808
1105	8	-0.01773356756520883
1105	9	0.041967691516212824
1105	10	-0.05177025446214145
1105	11	-0.026314365344148882
1105	12	-0.12983490136613085
1105	13	0.018114000299043198
1105	14	0.14327316903835197
1105	15	0.1808958390670412
1105	16	0.017942369856235985
1105	17	0.0718257764769561
1105	18	0.05818288510928937
1105	19	0.14280702018993452
1105	20	0.08711642508764328
1105	21	0.0781910869665694
1105	22	0.16652906322218541
1105	23	0.11934419685442066
1105	24	0.12995890882593386
1105	25	0.22805229064307753
1106	1	0.2766070190107719
1106	2	0.2818122534356604
1106	3	0.23036831452278506
1106	4	0.25307624684710817
1106	5	0.29723877532872933
1106	6	0.36923301271860964
1106	7	0.35187131931722604
1106	8	0.25359193008957703
1106	9	0.31871726776007137
1106	10	0.3252383400150265
1106	11	0.3310951828129376
1106	12	0.4671240647940209
1106	13	0.3419765964683492
1106	14	0.41187055306007636
1106	15	0.36415869877849616
1106	16	0.4122520540195467
1106	17	0.35744376741565276
1106	18	0.46579018613159207
1106	19	0.3919994049961204
1106	20	0.4397828883385344
1106	21	0.37731450612275097
1106	22	0.44348067561309534
1106	23	0.32501247063409267
1106	24	0.48924049177288964
1106	25	0.4978392719689353
1107	1	0.37268315061326973
1107	2	0.43696900598267874
1107	3	0.3982387226822297
1107	4	0.5129630959580425
1107	5	0.46949003620106566
1107	6	0.41987103096802514
1107	7	0.5085735063269681
1107	8	0.5382838915838235
1107	9	0.5437272835982014
1107	10	0.6275754001137983
1107	11	0.5062319411260816
1107	12	0.5360869481572408
1107	13	0.482433468456577
1107	14	0.5224758279433459
1107	15	0.47979482618744385
1107	16	0.6012004659007673
1107	17	0.5607123004604304
1107	18	0.6713792110195484
1107	19	0.5492979556013751
1107	20	0.6618028488742596
1107	21	0.5843271841144926
1107	22	0.6183629193106057
1107	23	0.6054203679820489
1107	24	0.5942889422197624
1107	25	0.6381949559102722
1108	1	0.3076535589157352
1108	2	0.4049173299443076
1108	3	0.4084003752001486
1108	4	0.5311243632142464
1108	5	0.5079851020028627
1108	6	0.4585707202102327
1108	7	0.4435246140668312
1108	8	0.47389777689576107
1108	9	0.5487821571589038
1108	10	0.618934556975276
1108	11	0.5641186382039365
1108	12	0.6296913038632397
1108	13	0.5691061114268976
1108	14	0.6051918555802516
1108	15	0.6126003109950915
1108	16	0.5451364539921286
1108	17	0.5521409768240986
1108	18	0.5805286520902442
1108	19	0.5622261470149894
1108	20	0.6624618787642689
1108	21	0.5983429528615218
1108	22	0.5225888430767611
1108	23	0.5468096187304639
1108	24	0.5986319435734302
1108	25	0.5774253544087955
1109	1	0.22492183323836912
1109	2	0.21189686246398054
1109	3	0.14915931567366414
1109	4	0.1697564989581366
1109	5	0.1765895838336533
1109	6	0.1685658958980909
1109	7	0.22453799593204593
1109	8	0.2249565491157881
1109	9	0.07552180337103209
1109	10	0.14347787160631498
1109	11	0.2134208305774905
1109	12	0.24315001454714746
1109	13	0.3544580136322679
1109	14	0.3940398904945326
1109	15	0.33757381835216904
1109	16	0.21047481675800128
1109	17	0.3147170474890686
1109	18	0.30731874447656793
1109	19	0.4262753878579417
1109	20	0.35632790776540446
1109	21	0.3439303128649911
1109	22	0.4258227378275947
1109	23	0.3275963475406414
1109	24	0.3389295498632592
1109	25	0.4886445686134877
1110	1	0.15461307078294695
1110	2	0.15788838030146835
1110	3	0.07567634603628193
1110	4	0.07701072903312678
1110	5	0.14402607767748066
1110	6	-0.005774828754482542
1110	7	0.08595589652345126
1110	8	0.08169715343317563
1110	9	0.08368294978372148
1110	10	0.06609794997093132
1110	11	0.04196484830492153
1110	12	0.051141297584287315
1110	13	0.04716672912761766
1110	14	0.14713411880852334
1110	15	0.08424809157171609
1110	16	0.09470283724432704
1110	17	0.15788288990868438
1110	18	0.06195201568564812
1110	19	0.08784480804784067
1110	20	0.11821284092068396
1110	21	0.045214744134526086
1110	22	0.02462562293684556
1110	23	-0.11340382855961906
1110	24	-0.0624583403088792
1110	25	-0.00954416683990189
1111	1	0.16794987122211325
1111	2	0.27268136519066744
1111	3	0.24459879843580978
1111	4	0.3192733045149154
1111	5	0.19099669395554741
1111	6	0.15922184860242794
1111	7	0.16481232944939705
1111	8	0.16333666110414669
1111	9	0.16149419476364102
1111	10	0.20137430719839955
1111	11	0.281265215240289
1111	12	0.26739407849209584
1111	13	0.21761109652526756
1111	14	0.2626299149426998
1111	15	0.31613968672932913
1111	16	0.331187625317483
1111	17	0.4513423392167084
1111	18	0.4445413658922277
1111	19	0.3296638829084344
1111	20	0.3647529582244289
1111	21	0.34187982171101794
1111	22	0.35878745010162305
1111	23	0.34223238536080913
1111	24	0.3056594546118063
1111	25	0.38989604003074874
1112	1	-0.5909679267071652
1112	2	-0.5426853345155926
1112	3	-0.5669897661059835
1112	4	-1.2053333291410837
1112	5	-0.6325808323261128
1112	6	-0.6164789052352617
1112	7	-0.5253870937675345
1112	8	-0.4796166880043202
1112	9	-0.3788535089847258
1112	10	-0.3887529277637043
1112	11	-0.44032182022630906
1112	12	-0.3962369401178023
1112	13	-0.4695616253514956
1112	14	-0.5439060139867564
1112	15	-0.3683968263574613
1112	16	-0.41644691703356074
1112	17	-0.391256810284915
1112	18	-0.4469218578609997
1112	19	-0.5159034076064586
1112	20	-0.48009461864322844
1112	21	-0.5070920107717427
1112	22	-0.4222256652426992
1112	23	-0.42507490236342704
1112	24	-0.4838693082687513
1112	25	-0.4930872326818019
1113	1	-0.6561495448971648
1113	2	-0.5687042219917622
1113	3	-0.45791744943545964
1113	4	-1.3463268240778632
1113	5	-0.7026970467041025
1113	6	-0.7090400636069347
1113	7	-0.4899899157109928
1113	8	-0.41790269523686163
1113	9	-0.387720781081768
1113	10	-0.34864871785981677
1113	11	-0.32840139929417234
1113	12	-0.4489219990806319
1113	13	-0.4021995766753008
1113	14	-0.4670406963943208
1113	15	-0.38851615381562965
1113	16	-0.3409724726910639
1113	17	-0.4625852143925755
1113	18	-0.5030114614111483
1113	19	-0.38961721395320836
1113	20	-0.3978586915861584
1113	21	-0.42559967295412093
1113	22	-0.534819403545896
1113	23	-0.49097061745433024
1113	24	-0.41104544234019613
1113	25	-0.4131571695754772
1114	1	-0.6772049480941114
1114	2	-0.5528203875279551
1114	3	-0.41726590795438767
1114	4	-1.338644841777338
1114	5	-0.7017331269482199
1114	6	-0.5458003876995718
1114	7	-0.4943463299366897
1114	8	-0.554324021417969
1114	9	-0.5248094846968314
1114	10	-0.3871049837514633
1114	11	-0.44424559895869464
1114	12	-0.49729684654234774
1114	13	-0.39074797640680714
1114	14	-0.4532442467811819
1114	15	-0.4734876117294391
1114	16	-0.5020607120435656
1114	17	-0.6084216020848743
1114	18	-0.6216421347930918
1114	19	-0.5770420228222051
1114	20	-0.731333962777299
1114	21	-0.6574469887834624
1114	22	-0.6060959243651212
1114	23	-0.6229415629545016
1114	24	-0.794221961290873
1114	25	-0.7412305374338501
1115	1	-0.23263505214354296
1115	2	-0.14255687899659364
1115	3	-0.21620247359004807
1115	4	0.013975108289287164
1115	5	-0.16760042834660993
1115	6	-0.07990962882432484
1115	7	-0.26262276595254264
1115	8	-0.23838578702430624
1115	9	-0.37705652987403937
1115	10	-0.3355206985361434
1115	11	-0.17495937030866315
1115	12	-0.30171185415309054
1115	13	-0.3591681757091152
1115	14	-0.266738764027167
1115	15	-0.3845427238639374
1115	16	-0.4478202987780193
1115	17	-0.35810453806407594
1115	18	-0.4540380314594543
1115	19	-0.38266069221408117
1115	20	-0.34621617950886474
1115	21	-0.2510948420362453
1115	22	-0.3094087755265704
1115	23	-0.3186517768746171
1115	24	-0.340948823982707
1115	25	-0.3642682806865878
1116	1	-0.0026528644664338685
1116	2	0.049767629140401226
1116	3	0.09412219647715148
1116	4	0.26697000748772837
1116	5	0.07832135936288154
1116	6	0.00916089178129198
1116	7	0.05349981521497682
1116	8	0.004442324059816372
1116	9	0.06968786329250776
1116	10	0.10795027547406022
1116	11	0.09370308617258871
1116	12	0.09061427328597915
1116	13	0.06707990136965947
1116	14	0.07067521054978698
1116	15	0.09691383835774303
1116	16	0.16051020077667033
1116	17	0.09829818236342192
1116	18	-0.05545896903536374
1116	19	-0.020112720389931837
1116	20	-0.05456934929268442
1116	21	-0.004935016920867952
1116	22	-0.11164179309599831
1116	23	-0.11402138257456329
1116	24	0.061959442554417876
1116	25	0.03079203831592281
1201	1	0.45284384878454276
1201	2	0.47109008301136157
1201	3	0.3907631918422183
1201	4	0.5758239630417208
1201	5	0.4669737041455022
1201	6	0.35313019195928774
1201	7	0.4130111205742253
1201	8	0.34292724731026425
1201	9	0.46391270159855225
1201	10	0.4670386211730886
1201	11	0.4671244773574088
1201	12	0.3714312903629775
1201	13	0.44609493406091616
1201	14	0.5215801679894696
1201	15	0.5652702876113977
1201	16	0.5785555984468687
1201	17	0.6386969980763766
1201	18	0.6292290100986264
1201	19	0.5586203391945048
1201	20	0.5745875511179364
1201	21	0.7297335765620261
1201	22	0.6907814132906935
1201	23	0.6450669631244708
1201	24	0.5441634148505585
1201	25	0.5195985871413242
1202	1	0.4186382301342384
1202	2	0.46066313261922076
1202	3	0.40506716186249747
1202	4	0.449273405086835
1202	5	0.425633495232141
1202	6	0.3961877279027135
1202	7	0.39129973225781356
1202	8	0.37840635744399975
1202	9	0.46655787913767455
1202	10	0.5806701249710216
1202	11	0.5021250604489254
1202	12	0.5669003262431929
1202	13	0.5321223952216343
1202	14	0.6299763919083787
1202	15	0.6338243088359263
1202	16	0.6591786341879313
1202	17	0.6538821712839535
1202	18	0.5508824812571618
1202	19	0.6041629230053402
1202	20	0.6045781530545895
1202	21	0.5468040463573942
1202	22	0.6983568011445556
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2210	16	-0.17586816814574036
2210	17	-0.179506330644152
2210	18	-0.21923399248155562
2210	19	-0.2614704291258505
2210	20	-0.26184188072030423
2210	21	-0.23281808885006683
2210	22	-0.14532332332660758
2210	23	-0.17285383502211715
2210	24	-0.09688345741982829
2210	25	-0.1535769229731585
2211	1	-0.8408545138412649
2211	2	-0.8339647464914179
2211	3	-0.6203613762815579
2211	4	-1.5636232353960153
2211	5	-0.979862333108521
2211	6	-0.8375985522197666
2211	7	-0.8924183146067861
2211	8	-0.8408107352644691
2211	9	-0.9221367576173094
2211	10	-0.798424425244221
2211	11	-0.9537895980519338
2211	12	-0.9658086450433601
2211	13	-0.9223255256056007
2211	14	-1.0821114348346228
2211	15	-1.0383781275906472
2211	16	-1.0110164685502738
2211	17	-1.148679072225562
2211	18	-0.9892994186230197
2211	19	-1.058056654946995
2211	20	-1.082636986962278
2211	21	-1.065890390690491
2211	22	-1.0445976989220185
2211	23	-1.1507532261499804
2211	24	-1.1905528612497918
2211	25	-1.2212015105002578
2212	1	-0.052426275413026246
2212	2	-0.30083953836620836
2212	3	-0.2641613263315108
2212	4	0.052044430271649844
2212	5	-0.09115520690139078
2212	6	-0.09143846230968222
2212	7	-0.06513518107365002
2212	8	-0.22557388877762463
2212	9	-0.17125638798693643
2212	10	-0.14923611355590793
2212	11	-0.2403363845987272
2212	12	-0.23267719316191204
2212	13	-0.23423861251868772
2212	14	-0.29399122171441405
2212	15	-0.2615757632882776
2212	16	-0.2548330686168754
2212	17	-0.21861330465979734
2212	18	-0.2896719627455582
2212	19	-0.34893868957903607
2212	20	-0.32840580148108955
2212	21	-0.3024767210268209
2212	22	-0.2532453013196161
2212	23	-0.2765690769658278
2212	24	-0.3219901739371025
2212	25	-0.28235077789952356
2213	1	-0.3294501415841822
2213	2	-0.3045586515814449
2213	3	-0.33001515381324253
2213	4	-0.1373404742295321
2213	5	-0.40379336546978095
2213	6	-0.43442984502974724
2213	7	-0.41663517404651174
2213	8	-0.5182534673099113
2213	9	-0.5829789605622224
2213	10	-0.5567674997694638
2213	11	-0.5908789299087118
2213	12	-0.5893564384070444
2213	13	-0.537096248709652
2213	14	-0.6426678107010773
2213	15	-0.6101743008403062
2213	16	-0.7331420478948516
2213	17	-0.7679558656302632
2213	18	-0.8673860947232583
2213	19	-0.8266779686749004
2213	20	-0.7930234046645879
2213	21	-0.8141741464839356
2213	22	-0.9314914378990906
2213	23	-0.8157187730922715
2213	24	-0.8832364570826101
2213	25	-0.8687926162793431
2214	1	-0.1948723709841218
2214	2	-0.21361917770834893
2214	3	-0.337094180514363
2214	4	-0.09150848671876588
2214	5	-0.19541292834661306
2214	6	-0.2515370442832605
2214	7	-0.36078802360149353
2214	8	-0.4115117934655288
2214	9	-0.37612154597709946
2214	10	-0.5195801792124755
2214	11	-0.5387350345598705
2214	12	-0.6341700868423032
2214	13	-0.5945808166108861
2214	14	-0.5451405353637213
2214	15	-0.520058021770538
2214	16	-0.6575359791322839
2214	17	-0.6986460034424979
2214	18	-0.6166638366124388
2214	19	-0.5762114168517627
2214	20	-0.6674088123591062
2214	21	-0.6611698219074214
2214	22	-0.9221357280225462
2214	23	-0.8696189668907195
2214	24	-0.8854189326783626
2214	25	-0.8729624231986648
2215	1	-0.4548576044028536
2215	2	-0.49729889313043074
2215	3	-0.4634038003391403
2215	4	-0.3565881412558838
2215	5	-0.5983604625054539
2215	6	-0.6251745454693349
2215	7	-0.6755805995890967
2215	8	-0.6501126648322
2215	9	-0.7385912023328558
2215	10	-0.7574251775519727
2215	11	-0.7080340370743414
2215	12	-0.7590828612200156
2215	13	-0.8301769753229777
2215	14	-0.931453673865164
2215	15	-0.9679697464756032
2215	16	-0.9862952799040272
2215	17	-1.0279219862998161
2215	18	-1.079675724995269
2215	19	-0.9482936827213848
2215	20	-0.815455650294389
2215	21	-0.8799877738794635
2215	22	-1.0727551040702394
2215	23	-0.9571366591132602
2215	24	-0.903781532308308
2215	25	-0.998909055943713
2216	1	-0.3284685655359354
2216	2	-0.2658637236416368
2216	3	-0.3947363927155223
2216	4	-0.14453880427403476
2216	5	-0.3513221290644495
2216	6	-0.45018515919828417
2216	7	-0.5475744701115062
2216	8	-0.45868940661152635
2216	9	-0.6564700975242275
2216	10	-0.5630582108401825
2216	11	-0.604183355775902
2216	12	-0.5856163348227525
2216	13	-0.6355719561491
2216	14	-0.8098513049405867
2216	15	-0.7643802705792595
2216	16	-0.7133141007450661
2216	17	-0.8201303225213792
2216	18	-0.7144826572967276
2216	19	-0.6608101652412302
2216	20	-0.6726094765102815
2216	21	-0.9212444110948965
2216	22	-0.7796143629592507
2216	23	-0.8303639384636199
2216	24	-0.8032374146404422
2216	25	-0.9321900790364523
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	49272.0
3	2.0
4	781.0
5	6592.0
6	7191.0
7	5593.0
8	4338.0
9	3895.0
10	3588.0
11	3260.0
12	2925.0
13	2885.0
14	2843.0
15	2830.0
16	2908.0
17	3081.0
18	3510.0
19	4128.0
20	5338.0
21	6776.0
22	8854.0
23	10769.0
24	12530.0
25	13968.0
26	15739.0
27	17371.0
28	19928.0
29	23666.0
30	29961.0
31	39174.0
32	49470.0
33	65288.0
34	86298.0
35	116060.0
36	167309.0
37	273382.0
38	1049803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.94649769611372	19.264891191200835	13.190150437445567	27.598460675239885
2	13.720099928952193	20.406136984131397	40.20127226019244	25.67249082672397
3	17.304556363960366	24.493644360445355	29.895006899438854	28.306792376155425
4	11.273116578409331	16.98929739367046	37.813251469999656	33.92433455792055
5	12.551709235571426	38.24487720000151	36.222225157864145	12.981188406562922
6	32.06541560489141	37.5930171888149	17.71100255393708	12.63056465235661
7	27.840722879180774	31.471434681469805	22.326137833503328	18.36170460584609
8	24.96478056452936	37.400258747338505	20.217511838822286	17.41744884930985
9	26.22458903789917	14.856393678804402	19.773324388630673	39.14569289466576
10	14.2685677783723	29.12170619197017	35.03237531320493	21.5773507164526
11	34.55404359389923	22.126935010790522	24.62756434008106	18.691457055229186
12	23.042278220246498	24.657651775675824	31.928930540398483	20.371139463679196
13	28.98365440912344	21.61885178281681	25.703175308041367	23.694318500018387
14	20.675821559726014	21.810236320694678	27.66691651699218	29.84702560258713
15	23.00167915425686	30.809510744795894	23.06560203949831	23.12320806144894
16	22.31780799186916	28.068039217350066	27.39317194219033	22.220980848590443
17	21.78129542671585	28.57136794914895	27.689980625793016	21.95735599834218
18	21.767258858833937	27.468043691069212	29.289510861467022	21.475186588629835
19	23.363578851895955	26.983942910640895	27.498908691155354	22.153569546307793
20	23.542383795642873	27.25123108123015	27.83068543623598	21.375699686891
21	22.953503632705473	26.673844709147854	27.11951817861258	23.253133479534092
22	23.099964078664893	27.96325087139879	26.941237032728015	21.9955480172083
23	22.14584436296984	27.636705813135055	27.494615023060287	22.722834800834818
24	22.888164178105374	27.024955381260412	28.011800277753423	22.07508016288079
25	22.623438457549618	27.272945835101115	27.65384434073446	22.44977136661481
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	116.0
1	116.0
2	222.0
3	328.0
4	328.0
5	328.0
6	816.0
7	1304.0
8	1304.0
9	1304.0
10	2039.0
11	2774.0
12	2774.0
13	2774.0
14	5426.5
15	8079.0
16	8079.0
17	8079.0
18	15311.5
19	22544.0
20	22544.0
21	22544.0
22	36700.5
23	50857.0
24	50857.0
25	50857.0
26	73750.0
27	96643.0
28	96643.0
29	96643.0
30	125072.0
31	153501.0
32	153501.0
33	153501.0
34	177628.5
35	201756.0
36	201756.0
37	201756.0
38	219227.0
39	236698.0
40	236698.0
41	236698.0
42	250060.0
43	263422.0
44	263422.0
45	263422.0
46	274228.5
47	285035.0
48	285035.0
49	285035.0
50	274833.5
51	264632.0
52	264632.0
53	264632.0
54	235626.5
55	206621.0
56	206621.0
57	206621.0
58	177339.5
59	148058.0
60	148058.0
61	148058.0
62	120433.5
63	92809.0
64	92809.0
65	92809.0
66	71145.5
67	49482.0
68	49482.0
69	49482.0
70	36011.0
71	22540.0
72	22540.0
73	22540.0
74	15698.5
75	8857.0
76	8857.0
77	8857.0
78	6167.5
79	3478.0
80	3478.0
81	3478.0
82	2363.5
83	1249.0
84	1249.0
85	1249.0
86	828.5
87	408.0
88	408.0
89	408.0
90	260.0
91	112.0
92	112.0
93	112.0
94	57.5
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18922305409969142
2	0.009381013394578622
3	0.0056097517284163624
4	0.002215616228870328
5	0.08306203819722378
6	0.34483473859971164
7	0.031820020308244074
8	0.014990765122994985
9	0.005892596353378532
10	0.0042898101452595715
11	0.0
12	4.714077082702826E-5
13	0.0
14	0.0016970677497730171
15	0.0
16	0.0
17	0.020977643018027573
18	0.028190180954562896
19	0.0
20	0.0
21	0.0010370969581946215
22	1.8856308330811303E-4
23	0.005986877895032589
24	0.0
25	2.8284462496216956E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2121306.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.489336125402
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.50739703794554	53.659014431496985
2	13.239686900139075	17.870753587971357
3	3.820601214580828	7.735495187158939
4	1.4596894202824575	3.940538796965439
5	0.6825615164365011	2.3032811804523567
6	0.3503253022162656	1.4185933244703957
7	0.21629122101774403	1.0218145641368102
8	0.14697055030350117	0.7935155895974636
9	0.10207361492872115	0.6199992456713382
>10	0.4318281013669192	5.074727323420506
>50	0.025083456216663764	1.1565963746445669
>100	0.01413561142280964	1.8350619408920508
>500	0.002307286535704094	1.0366049249808862
>1k	0.001048766607138121	1.534003528140976
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3309	0.1559888106666365	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3145	0.14825772425100386	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3108	0.1465135157304038	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2918	0.13755676927326843	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2757	0.1299671051701169	No Hit
GTACATGGGAAGCAGTGGTATCAAC	2446	0.11530632544291113	No Hit
CCCATGTACTCTGCGTTGATACCAC	2324	0.10955515140201368	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2179	0.10271973963209458	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2162	0.10191834652803508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.714077082702826E-5	0.0	0.0	9.428154165405651E-5	0.0
7	4.714077082702826E-5	0.0	0.0	9.428154165405651E-5	0.0
8	4.714077082702826E-5	0.0	0.0	9.428154165405651E-5	0.0
9	4.714077082702826E-5	0.0	0.0	9.428154165405651E-5	0.0
10	4.714077082702826E-5	0.0	0.0	9.428154165405651E-5	0.0
11	4.714077082702826E-5	0.0	0.0	1.4142231248108478E-4	0.0
12	4.714077082702826E-5	0.0	0.0	1.4142231248108478E-4	0.0
13	9.428154165405651E-5	0.0	0.0	1.4142231248108478E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	695	0.0	12.191198	4
TGCGCAT	75	2.0999055E-4	11.385065	10
CCGATCC	60	0.005933435	11.069075	9
AAAGGCG	150	2.060915E-9	10.789439	5
ACTAGAC	125	2.1724918E-7	10.664019	3
TAACCTA	110	5.896827E-6	10.385556	5
GGCGAGG	650	0.0	10.363328	19
GTAGGAC	1675	0.0	10.288844	3
GGACGTG	1515	0.0	10.242014	6
AATACCG	75	0.0026022336	10.154766	5
AGGACGT	1590	0.0	10.118841	5
AGGCGTG	170	1.5861588E-9	10.049198	7
TGTAGGA	1805	0.0	10.022811	2
ATACCGT	95	1.6109228E-4	10.02044	6
CGTGCAC	95	1.6663597E-4	9.986899	10
GTCCTAC	1900	0.0	9.975529	1
GGACCTG	1195	0.0	9.95755	6
TGTACCG	135	6.2609433E-7	9.872689	5
CTGTAGG	1815	0.0	9.865467	1
TAGAGTG	310	0.0	9.827193	5
>>END_MODULE