FastQCFastQC Report
Thu 26 May 2016
SRR1513187_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513187_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400158
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGTA701.08114975E-412.226925
GTCCTAA8100.010.965741
CAATACG700.001456421910.8887634
GGCGTAA750.002679150310.1194956
GGACGTG10050.010.0062186
CAGCGTA951.6642566E-49.9885479
CTATACT6050.09.763894
TTAGGAC9750.09.5768113
CTAAGAC4800.09.5279493
GTAGGAC12900.09.2325093
GTATAAG4350.09.2214681
AGGACGT10750.09.2002065
CTAAAGT9950.09.1925244
TAGGACG11450.09.1532184
AGCGCCG1054.5467104E-49.03725810
TAGGACC6750.09.0336414
TGCGTTG7250.09.03155612
AGCACCG1901.0404619E-89.009315
GTATTAA5000.08.9777571
GTCCTAC12650.08.9090521
Produced by FastQC (version 0.11.5)