##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513187_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3400158
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.385349739629746	31.0	30.0	34.0	25.0	34.0
2	29.27917173260772	31.0	30.0	34.0	22.0	34.0
3	29.443771730607814	31.0	30.0	34.0	23.0	34.0
4	32.54727956759656	37.0	33.0	37.0	22.0	37.0
5	32.6732625366233	37.0	33.0	37.0	25.0	37.0
6	33.029109235511996	37.0	35.0	37.0	26.0	37.0
7	32.98846435959741	37.0	35.0	37.0	26.0	37.0
8	32.86351398964401	37.0	35.0	37.0	25.0	37.0
9	34.115135826041026	39.0	35.0	39.0	24.0	39.0
10	33.70045186135468	39.0	34.0	39.0	18.0	39.0
11	34.14362538446743	39.0	35.0	39.0	25.0	39.0
12	34.139650275075454	39.0	35.0	39.0	25.0	39.0
13	34.17961635900449	39.0	35.0	39.0	25.0	39.0
14	34.41394105803319	39.0	34.0	41.0	17.0	41.0
15	34.82865855057324	40.0	34.0	41.0	21.0	41.0
16	34.662339220706805	39.0	34.0	41.0	18.0	41.0
17	34.26772138235929	39.0	33.0	41.0	17.0	41.0
18	33.99605165407019	39.0	33.0	41.0	16.0	41.0
19	34.63103361667311	39.0	34.0	41.0	18.0	41.0
20	34.838699260446134	40.0	34.0	41.0	22.0	41.0
21	34.885053871025995	40.0	35.0	41.0	22.0	41.0
22	34.56951971055463	40.0	34.0	41.0	18.0	41.0
23	34.101735566406035	39.0	34.0	41.0	10.0	41.0
24	34.33925746979993	39.0	34.0	41.0	15.0	41.0
25	33.97941772117649	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9006535507922955
1101	2	1.1361884130827207
1101	3	1.0362312614232678
1101	4	1.391280116647799
1101	5	1.2842956377873662
1101	6	1.0662531906832697
1101	7	0.9802985791115049
1101	8	0.9754037460794294
1101	9	1.2944511767345048
1101	10	1.65035304999342
1101	11	1.300163763717869
1101	12	1.123725234683974
1101	13	1.0694109348332574
1101	14	2.065432537097358
1101	15	1.7351293198397784
1101	16	1.7963037332024143
1101	17	2.1584171428395322
1101	18	2.079683355698421
1101	19	1.6284378035183167
1101	20	1.3137540607728297
1101	21	1.3080370073596725
1101	22	1.586509212134878
1101	23	1.8725710952580386
1101	24	1.5036995732099072
1101	25	1.749505628215097
1102	1	0.845433282548786
1102	2	1.118130228090589
1102	3	0.998834987573936
1102	4	1.2560361220077567
1102	5	1.1987705836961027
1102	6	0.9311410435058534
1102	7	0.8861355185292865
1102	8	0.9486692911718748
1102	9	1.271921549472502
1102	10	1.4915638338250474
1102	11	1.1546166121148005
1102	12	0.9976297328645387
1102	13	0.9676780166094119
1102	14	1.9940494295540816
1102	15	1.6226800304023286
1102	16	1.706035858497458
1102	17	2.0367368406660447
1102	18	2.206306554705108
1102	19	1.6398215564687462
1102	20	1.4071097477319228
1102	21	1.2821320543207264
1102	22	1.5122729439405376
1102	23	1.792697963865436
1102	24	1.624322329651683
1102	25	1.8726050577981042
1103	1	0.8892392550898123
1103	2	1.1680249981872883
1103	3	1.0202156970020475
1103	4	1.428886616183867
1103	5	1.262627507655857
1103	6	0.9350421426585172
1103	7	0.9363174388262152
1103	8	0.956450313771505
1103	9	1.3866712869700635
1103	10	1.6205347937293269
1103	11	1.2300814474708233
1103	12	1.1857799224157262
1103	13	1.0905818506712421
1103	14	2.052350268035717
1103	15	1.6993480655410238
1103	16	1.7381489115839983
1103	17	2.107321496177377
1103	18	2.3786505870478507
1103	19	1.7361843714716798
1103	20	1.4942870376078687
1103	21	1.472830654205957
1103	22	1.638097308440443
1103	23	2.021828409264927
1103	24	1.7655595173958716
1103	25	1.9659564203689186
1104	1	0.7681274709480697
1104	2	1.0212100362035557
1104	3	0.8406975996801975
1104	4	1.250222660445921
1104	5	1.1193767170326723
1104	6	0.9246655018108285
1104	7	0.8170574026436128
1104	8	0.8875932153771018
1104	9	1.2018671369214644
1104	10	1.3269412582368716
1104	11	0.9222613097013195
1104	12	0.8803365446110902
1104	13	0.8952827782392987
1104	14	1.800550339468046
1104	15	1.332058903992646
1104	16	1.39534277445118
1104	17	1.8756585622414903
1104	18	1.959316600215999
1104	19	1.3404887254362734
1104	20	1.10573067362634
1104	21	1.062750165406186
1104	22	1.342297598203615
1104	23	1.6884531165000851
1104	24	1.4450801026562559
1104	25	1.783180718890847
1105	1	0.7572930496819907
1105	2	1.0245627208512076
1105	3	0.8866977107882228
1105	4	1.2756691758204965
1105	5	1.1300416985609658
1105	6	0.7778237474151197
1105	7	0.7898276034713732
1105	8	0.8569968430541124
1105	9	1.1110654925226982
1105	10	1.5342007788057472
1105	11	1.0049506794410519
1105	12	1.0172341307892552
1105	13	0.943800874958832
1105	14	1.686806276803118
1105	15	1.2835255299197001
1105	16	1.5114020505823973
1105	17	1.7951594094401742
1105	18	1.5223787373788582
1105	19	1.2828958807930704
1105	20	1.2266787028477495
1105	21	1.1452270410485283
1105	22	1.321405122994598
1105	23	1.5673842573017254
1105	24	1.3537451397385567
1105	25	1.6297599805780223
1106	1	0.5184352461183046
1106	2	0.7965652810890589
1106	3	0.707209551888301
1106	4	1.0607888256460285
1106	5	1.0229690414883095
1106	6	0.7106505390654405
1106	7	0.6882315842282907
1106	8	0.6590584539392523
1106	9	0.879856399548899
1106	10	0.8121383420374286
1106	11	0.7026836301151818
1106	12	0.7053579306777564
1106	13	0.6510645175165948
1106	14	1.6332260732229145
1106	15	1.2248981477629073
1106	16	1.3640077120115919
1106	17	1.6525789374479416
1106	18	1.7749513634808949
1106	19	1.3381361095627113
1106	20	1.0957607336944832
1106	21	0.8605141386885649
1106	22	1.0882830781078425
1106	23	1.4933138920548927
1106	24	1.1397526139813223
1106	25	1.4143520975230857
1107	1	0.5151454827354982
1107	2	0.7006741827535521
1107	3	0.6746908168778454
1107	4	0.8129470128302358
1107	5	0.7669194610419439
1107	6	0.5807174300675157
1107	7	0.5089069662504642
1107	8	0.6539991306730215
1107	9	0.9009528712840691
1107	10	1.1120848865826147
1107	11	0.6724147348407641
1107	12	0.5716696271818549
1107	13	0.6029208145077263
1107	14	1.4348607656082777
1107	15	0.8472502082556375
1107	16	1.0847326580953123
1107	17	1.5174088323598411
1107	18	1.5317817827068296
1107	19	1.0431751566219418
1107	20	0.8579761243596522
1107	21	0.7459417925675709
1107	22	1.0697830873243035
1107	23	1.319945665895304
1107	24	1.078113456734215
1107	25	1.368718855193734
1108	1	0.7690760734484883
1108	2	0.9222416222491141
1108	3	0.7457617872076732
1108	4	1.2313136095641042
1108	5	1.1771227390304375
1108	6	0.9469516710884989
1108	7	0.8416623780334191
1108	8	0.8803869552872001
1108	9	1.1345442250923412
1108	10	1.3883936375440555
1108	11	1.0099666485066052
1108	12	0.8543676472616113
1108	13	0.8962046471973153
1108	14	1.8257236582824845
1108	15	1.3682493274534124
1108	16	1.492495170105272
1108	17	1.8218867807956514
1108	18	2.1175130312734822
1108	19	1.5165354933583046
1108	20	1.2228213250234319
1108	21	1.2158339525645303
1108	22	1.4432714295716451
1108	23	1.8564798947444743
1108	24	1.5209028201193746
1108	25	1.895034152463765
1109	1	0.44918984316802835
1109	2	0.63364054582145
1109	3	0.6384387570280481
1109	4	0.7969669291475867
1109	5	0.9365798805270202
1109	6	0.5021329312597587
1109	7	0.52491561068652
1109	8	0.5810338250373093
1109	9	0.8430011937738016
1109	10	1.1934894086322743
1109	11	0.6710760890872436
1109	12	0.6903621756776914
1109	13	0.6207697727101262
1109	14	1.4922490795564372
1109	15	1.0440937299744562
1109	16	1.1925538961596729
1109	17	1.5989708527381765
1109	18	1.6663153928069505
1109	19	1.1279904016822115
1109	20	0.9823725398374279
1109	21	0.8879872049578381
1109	22	1.08088313589559
1109	23	1.5515857295227562
1109	24	1.3082318048609167
1109	25	1.5268220739726388
1110	1	0.45705077989298104
1110	2	0.6952818009187283
1110	3	0.5808228962731832
1110	4	1.024346991637124
1110	5	0.8849596476715504
1110	6	0.5442284564543911
1110	7	0.6137618169239971
1110	8	0.5956127150977508
1110	9	0.7524345629285918
1110	10	1.1551313208411003
1110	11	0.7419091271643694
1110	12	0.5948641890142099
1110	13	0.5549627855541743
1110	14	1.393417015425186
1110	15	1.0927192035609679
1110	16	1.1831509139743233
1110	17	1.5303158730668969
1110	18	1.0976960214780078
1110	19	0.8860503100098214
1110	20	0.6696979057819661
1110	21	0.5488793173636921
1110	22	0.8624879905647163
1110	23	-0.27090567163975976
1110	24	0.46288631247746537
1110	25	0.8566495573259587
1111	1	0.19704477301332446
1111	2	0.518955778746296
1111	3	0.5190292322405412
1111	4	0.8852245541974497
1111	5	0.7222695562672143
1111	6	0.4108049501598998
1111	7	0.42620062750601306
1111	8	0.48185546070918406
1111	9	0.6253111989922644
1111	10	0.9693087428571872
1111	11	0.5773131944707259
1111	12	0.4842567790642107
1111	13	0.31334672562770294
1111	14	1.2177388083636487
1111	15	0.8745604272900209
1111	16	0.887843152088152
1111	17	1.28610544809586
1111	18	1.5529345821570857
1111	19	0.94686117597373
1111	20	0.587456851305987
1111	21	0.3532951413404888
1111	22	0.7440965436357772
1111	23	1.140109766599089
1111	24	0.9011989025414167
1111	25	1.0284059905650054
1112	1	-0.031472682797161866
1112	2	0.24087799510493468
1112	3	0.1369754960575129
1112	4	0.4870277578983533
1112	5	0.24740262187808781
1112	6	0.020315745872757418
1112	7	-0.14312278805935108
1112	8	0.015103748470814082
1112	9	0.21349387224955052
1112	10	0.5023595967568966
1112	11	0.07874439818474599
1112	12	0.05163668107815056
1112	13	-0.09530416227721616
1112	14	0.5577208252143606
1112	15	0.3696934489647177
1112	16	0.259660699209185
1112	17	0.7148292086927626
1112	18	0.8473201517293703
1112	19	0.29174127517745063
1112	20	0.19659119064772312
1112	21	0.1469714902632404
1112	22	0.4839925641264955
1112	23	0.8422318981678671
1112	24	0.5218511121582878
1112	25	0.8312201937240999
1113	1	0.07345796711451413
1113	2	0.19375045989810147
1113	3	0.261593753712436
1113	4	0.47041277253592284
1113	5	0.49226603557403337
1113	6	0.1922126030715816
1113	7	0.1006601664166098
1113	8	0.11010286658267177
1113	9	0.22688853890912242
1113	10	0.49767078805039944
1113	11	0.1648662086523558
1113	12	0.025260124589891575
1113	13	-0.029818498942226768
1113	14	0.529897617640934
1113	15	0.33140252159950734
1113	16	0.4261715685913572
1113	17	0.6639322265228529
1113	18	0.7544819875102746
1113	19	0.3955668616330641
1113	20	0.26825301357698805
1113	21	0.17449689440288552
1113	22	0.30148252433960465
1113	23	0.40746909062599457
1113	24	0.2717216474333739
1113	25	0.7320504803333137
1114	1	-1.1734927192911506
1114	2	-0.8816074506957463
1114	3	-0.9585518461721279
1114	4	-0.6354454540314052
1114	5	-3.9073745456780884
1114	6	-1.560340749507013
1114	7	-1.3213503637147426
1114	8	-0.916823463352749
1114	9	-0.5998610197276477
1114	10	-0.498782137985728
1114	11	-0.8717789266164573
1114	12	-1.0158067618239386
1114	13	-0.9152451761442393
1114	14	-6.402765938838343
1114	15	-2.7808178027535746
1114	16	-1.5719993143871918
1114	17	-0.7382532147056438
1114	18	-6.803391193686391
1114	19	-2.8232466253494373
1114	20	-1.769008317788355
1114	21	-1.4838933706584427
1114	22	-1.1992014213390405
1114	23	-0.8657298735720786
1114	24	-0.9157774854867142
1114	25	-0.8360119807132875
1115	1	-1.058283585278339
1115	2	-0.6823177234049851
1115	3	-0.6790906757755124
1115	4	-3.3469556927288266
1115	5	-1.3537188829133342
1115	6	-0.9061076588820924
1115	7	-0.8322733464625784
1115	8	-0.6423709376257918
1115	9	-0.44049726152514523
1115	10	-0.8002431368508951
1115	11	-0.9013666290631335
1115	12	-0.8357624213126655
1115	13	-0.9290818009059478
1115	14	-6.439086802767893
1115	15	-2.848040461984265
1115	16	-1.581279312973713
1115	17	-0.7139115770661988
1115	18	-0.47155698140775826
1115	19	-0.7317953331029159
1115	20	-0.735598703266632
1115	21	-0.9497264043025311
1115	22	-2.200939189508766
1115	23	-1.2917849116089926
1115	24	-0.9705044836164376
1115	25	-0.586911140466853
1116	1	-0.4209622021953088
1116	2	-0.17658747321237556
1116	3	-0.15786546881432528
1116	4	0.020970607736103375
1116	5	-0.7262554872637814
1116	6	-0.3914164151321273
1116	7	-0.3276979085423335
1116	8	-0.3248300907413224
1116	9	-0.35583171965010507
1116	10	-0.14703788392898076
1116	11	-0.40896818880852237
1116	12	-0.7524894172807493
1116	13	-0.7537264611511745
1116	14	0.08767778948565308
1116	15	-0.30330905494638216
1116	16	-0.26842192412346577
1116	17	0.18426398082821294
1116	18	-0.012858749827138638
1116	19	-0.2317921480681875
1116	20	-0.662773460226461
1116	21	-0.7941394908376864
1116	22	-0.6730126355963932
1116	23	-1.3842072667307406
1116	24	-1.0940010879289304
1116	25	-0.499581621991922
1201	1	0.7284656664982876
1201	2	0.948060161305083
1201	3	0.8971447847144915
1201	4	1.266354871702518
1201	5	1.0612209829440005
1201	6	0.8117758797165422
1201	7	0.6991617443546403
1201	8	0.8919845978113585
1201	9	0.923494885329113
1201	10	1.3249692535847686
1201	11	0.8712222952926609
1201	12	0.8863073691826671
1201	13	0.7461786469205123
1201	14	1.7507288576039954
1201	15	1.4340618768322955
1201	16	1.5898777648756592
1201	17	1.7803045006704394
1201	18	1.3192639881027475
1201	19	1.1225879659237208
1201	20	1.0824878580806185
1201	21	1.0522406952295995
1201	22	1.312643118168907
1201	23	1.5860010992434397
1201	24	1.1996286715275346
1201	25	1.5959622808885499
1202	1	0.9189254850872857
1202	2	1.0883227960646025
1202	3	0.9844771690585397
1202	4	1.4984565307541473
1202	5	1.2753308279213158
1202	6	0.9177186196006559
1202	7	0.887657414583181
1202	8	0.9566906383205733
1202	9	1.2173896979325605
1202	10	1.5388832214573895
1202	11	1.1906441551249003
1202	12	0.9828921922605929
1202	13	0.9852228689313591
1202	14	1.8902670591859376
1202	15	1.5247444402916415
1202	16	1.5333690815484715
1202	17	1.9743280115635429
1202	18	2.1569932501454403
1202	19	1.5914878221087818
1202	20	1.4048881855326911
1202	21	1.2513409165079281
1202	22	1.4394877388154228
1202	23	1.7490201486585022
1202	24	1.4371255433788264
1202	25	1.8183371319078248
1203	1	0.7944168109930594
1203	2	0.9270033414806811
1203	3	0.8042728987581462
1203	4	1.1355948246006378
1203	5	1.0661443259561594
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2211	17	-2.139495371815279
2211	18	-0.7734487246421082
2211	19	-0.6828927692690172
2211	20	-0.7764853155385296
2211	21	-0.7400112176488278
2211	22	-0.7064815795465691
2211	23	-0.2900415766650113
2211	24	-0.710081927095608
2211	25	-7.7399381342078755
2212	1	-1.6672855101424702
2212	2	-4.0414674779658775
2212	3	-1.92606575525393
2212	4	-3.7545324543811347
2212	5	-1.4576829112947927
2212	6	-1.273558196589775
2212	7	-1.1889214031464874
2212	8	-1.0539700957816756
2212	9	-1.1512925329500803
2212	10	-0.6065904808379727
2212	11	-1.175012187407063
2212	12	-1.2617698146081366
2212	13	-1.418214301810437
2212	14	-6.813334379075243
2212	15	-5.404744121464706
2212	16	-3.1174770627229442
2212	17	-7.631105614365829
2212	18	-3.2603177764652003
2212	19	-2.117566877046734
2212	20	-2.0712877647908456
2212	21	-1.6327803574506774
2212	22	-1.6312828108408794
2212	23	-1.0907150598139808
2212	24	-1.408003845780918
2212	25	-1.234914295571663
2213	1	-0.2984231874421859
2213	2	-0.2303820124328766
2213	3	-0.27735592510656915
2213	4	0.1383275536413322
2213	5	-0.04944410493205709
2213	6	-0.3678273962504619
2213	7	-0.40684733345627677
2213	8	-0.4334684288190971
2213	9	-0.15545040449861602
2213	10	-4.229371973936683
2213	11	-1.7224387522805102
2213	12	-1.1135876915488154
2213	13	-0.8938355495006931
2213	14	-0.07844817310837016
2213	15	-0.5648185593991215
2213	16	-0.3720186315747469
2213	17	-0.06625912616608076
2213	18	0.1086129344433786
2213	19	-0.4410674327009261
2213	20	-0.8586717843077878
2213	21	-0.8498491336305349
2213	22	-1.0896886422507919
2213	23	-1.3941025776508127
2213	24	-0.9094554512598663
2213	25	-0.5202762598914248
2214	1	-1.034792522942503
2214	2	-0.7869376900504008
2214	3	-0.721562392382058
2214	4	-0.3765671728230373
2214	5	-3.677383500181598
2214	6	-1.5537370341456622
2214	7	-1.2727410465224303
2214	8	-0.9440801885618413
2214	9	-0.7688927027049601
2214	10	-4.341336983857765
2214	11	-1.9522707849660392
2214	12	-1.3943246750809308
2214	13	-1.138035662566324
2214	14	-0.2957017853456634
2214	15	-0.6386692171253472
2214	16	-0.6478394874016544
2214	17	-0.36595504119522815
2214	18	0.07809412303436147
2214	19	-0.7454023139721926
2214	20	-1.094954093335943
2214	21	-1.064588673258207
2214	22	-0.7822497294626558
2214	23	-0.4660992145050997
2214	24	-0.9870598420843919
2214	25	-0.6524283980175412
2215	1	-2.374274150692873
2215	2	-4.95377277270785
2215	3	-3.19085475803465
2215	4	-2.0033653539947274
2215	5	-2.027264614139245
2215	6	-2.1840787766236716
2215	7	-2.323716326751054
2215	8	-2.4597452259945563
2215	9	-8.333013799179021
2215	10	-3.9904108601068167
2215	11	-3.2157024029224637
2215	12	-3.657385043794438
2215	13	-2.87239985887852
2215	14	-8.026722034028246
2215	15	-4.946385740978087
2215	16	-9.527914970991542
2215	17	-4.784517844616069
2215	18	-3.277230777844572
2215	19	-3.7115136103162065
2215	20	-3.5766450761680098
2215	21	-3.4795862798258312
2215	22	-9.433429626588204
2215	23	-10.771722724566079
2215	24	-6.954895671812121
2215	25	-5.31629886342262
2216	1	-2.030661296136291
2216	2	-2.0172286211230173
2216	3	-1.868365514209163
2216	4	-7.171349490876299
2216	5	-3.7376761289959255
2216	6	-2.8622870552048276
2216	7	-2.4415299898832963
2216	8	-8.14463850532593
2216	9	-4.121194649320476
2216	10	-2.336420003958416
2216	11	-2.3715288306887885
2216	12	-2.5826565635418213
2216	13	-2.3957756856126053
2216	14	-1.7902982809890773
2216	15	-2.3865295183357844
2216	16	-2.0120654811148313
2216	17	-1.7169852412194686
2216	18	-7.8031534569536305
2216	19	-5.103206058747055
2216	20	-4.427504427926458
2216	21	-3.601147795487247
2216	22	-3.201705258784404
2216	23	-2.4113483053371993
2216	24	-2.795140196637636
2216	25	-2.2327785376664195
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	159422.0
3	2.0
4	1388.0
5	7822.0
6	9248.0
7	8531.0
8	7707.0
9	7282.0
10	6802.0
11	6731.0
12	6616.0
13	6458.0
14	6732.0
15	6822.0
16	6589.0
17	7280.0
18	8264.0
19	10006.0
20	12729.0
21	16343.0
22	20090.0
23	24077.0
24	27828.0
25	31961.0
26	35734.0
27	40474.0
28	45214.0
29	54221.0
30	64529.0
31	77077.0
32	98519.0
33	127686.0
34	168316.0
35	225720.0
36	319733.0
37	462487.0
38	1273718.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.85502166269827	18.684217193812948	12.863188261309944	25.59757288217884
2	14.78732206072536	19.9851332727499	40.405181711979424	24.822362954545323
3	17.754274246481792	24.809442117287528	29.55296963635216	27.883313999878517
4	11.579804983646229	16.998793554912748	38.56042505624563	32.860976405195395
5	13.352587435887228	37.45132294555335	35.81796764544145	13.37812197311797
6	32.424926651884284	36.560954387385124	18.179331215793187	12.834787744937406
7	28.423185433520093	31.244456955974222	22.582173835155498	17.750183775350187
8	25.858906280702538	35.34805721688362	20.74212044161061	18.050916060803235
9	26.721003621679245	14.671389675272566	19.920324233246497	38.687282469801694
10	14.218438459533605	28.743457006716994	35.081798814980594	21.95630571876881
11	34.65270954798703	22.107305277897076	24.05484988792338	19.185135286192512
12	22.96709878718736	24.448003445510512	31.48802985167713	21.096867915625
13	28.8303384593479	21.494370582228687	25.58104164012854	24.09424931829488
14	21.21271580939112	20.684224027563356	28.455803789961216	29.647256373084307
15	23.919563835797007	29.210849967046414	23.13359146972978	23.735994727426796
16	23.29275707814486	27.129562107674698	26.70419830542827	22.87348250875217
17	22.48073830278158	27.327012511202764	27.588670302943996	22.603578883071656
18	22.384574106283377	27.004051703645477	28.3279161557417	22.28345803432945
19	23.851946819423684	26.733644954667422	26.793452951287815	22.620955274621082
20	24.171464950463825	26.63379945233077	26.750799910094695	22.44393568711071
21	24.147953361069245	26.197696179687746	26.324028828792784	23.33032163045023
22	23.88340413186446	26.60024933715568	26.413820234207446	23.102526296772417
23	23.367012460217857	26.393456223313088	26.787820912243042	23.451710404226013
24	23.654313954716695	26.514394267304326	26.59646058477474	23.23483119320424
25	23.61164273876391	26.301490692111045	26.697325686525396	23.38954088259965
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	138.0
1	138.0
2	294.0
3	450.0
4	450.0
5	450.0
6	792.5
7	1135.0
8	1135.0
9	1135.0
10	1947.0
11	2759.0
12	2759.0
13	2759.0
14	5944.5
15	9130.0
16	9130.0
17	9130.0
18	18999.0
19	28868.0
20	28868.0
21	28868.0
22	49041.0
23	69214.0
24	69214.0
25	69214.0
26	102103.5
27	134993.0
28	134993.0
29	134993.0
30	176111.0
31	217229.0
32	217229.0
33	217229.0
34	258790.5
35	300352.0
36	300352.0
37	300352.0
38	336252.0
39	372152.0
40	372152.0
41	372152.0
42	399195.0
43	426238.0
44	426238.0
45	426238.0
46	443259.0
47	460280.0
48	460280.0
49	460280.0
50	449431.0
51	438582.0
52	438582.0
53	438582.0
54	398540.0
55	358498.0
56	358498.0
57	358498.0
58	308559.0
59	258620.0
60	258620.0
61	258620.0
62	211362.0
63	164104.0
64	164104.0
65	164104.0
66	127283.5
67	90463.0
68	90463.0
69	90463.0
70	66219.5
71	41976.0
72	41976.0
73	41976.0
74	29038.5
75	16101.0
76	16101.0
77	16101.0
78	11111.5
79	6122.0
80	6122.0
81	6122.0
82	4038.0
83	1954.0
84	1954.0
85	1954.0
86	1274.5
87	595.0
88	595.0
89	595.0
90	378.0
91	161.0
92	161.0
93	161.0
94	94.0
95	27.0
96	27.0
97	27.0
98	22.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17187436583829338
2	0.2556351793063734
3	0.2588115022890113
4	0.44133243219873897
5	0.25507638174461306
6	0.01811680516023079
7	0.018440319538092054
8	0.017705059588407363
9	0.018528550732054217
10	0.021175486550919106
11	0.020528457795196575
12	0.027998698883993037
13	0.028263392465879528
14	0.02535176306512815
15	0.04229215230586343
16	0.021028434560982166
17	0.04246861469378776
18	0.032174975398202084
19	0.0278516468940561
20	0.030175068335059722
21	0.03514542559492823
22	0.04549788568648869
23	0.029998605947135396
24	0.04961534140472296
25	0.03726297425002015
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3400158.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.1456641934534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.37770524494607	56.38147520752366
2	12.579679391489956	17.648199325135216
3	3.7036636609207343	7.793878423333263
4	1.4323121955658595	4.018819611614029
5	0.6566084762419524	2.3029118840521563
6	0.36758854543027225	1.547084560146712
7	0.2227846766751451	1.0939165382251272
8	0.1371127389037658	0.7694291311830568
9	0.09254679085408746	0.5842580502089325
>10	0.3991761061738275	4.678218357360149
>50	0.019652430907865413	0.9454546882769199
>100	0.009731774095341983	1.2438209191664107
>500	0.0010150360946177277	0.5047216994631515
>1k	4.229317006541347E-4	0.48781160431114434
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	2.9410397987387646E-5
3	0.0	0.0	0.0	0.0	2.9410397987387646E-5
4	0.0	0.0	0.0	0.0	2.9410397987387646E-5
5	2.9410397987387646E-5	0.0	0.0	0.0	2.9410397987387646E-5
6	8.823119396216293E-5	0.0	0.0	0.0	2.9410397987387646E-5
7	1.1764159194955058E-4	0.0	0.0	0.0	2.9410397987387646E-5
8	1.4705198993693823E-4	2.9410397987387646E-5	0.0	0.0	2.9410397987387646E-5
9	1.4705198993693823E-4	2.9410397987387646E-5	0.0	2.9410397987387646E-5	2.9410397987387646E-5
10	1.4705198993693823E-4	2.9410397987387646E-5	0.0	2.9410397987387646E-5	2.9410397987387646E-5
11	2.058727859117135E-4	2.9410397987387646E-5	0.0	2.9410397987387646E-5	2.9410397987387646E-5
12	2.058727859117135E-4	2.9410397987387646E-5	0.0	2.9410397987387646E-5	2.9410397987387646E-5
13	2.058727859117135E-4	2.9410397987387646E-5	0.0	2.9410397987387646E-5	2.9410397987387646E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGCGTA	70	1.08114975E-4	12.22692	5
GTCCTAA	810	0.0	10.96574	1
CAATACG	70	0.0014564219	10.888763	4
GGCGTAA	75	0.0026791503	10.119495	6
GGACGTG	1005	0.0	10.006218	6
CAGCGTA	95	1.6642566E-4	9.988547	9
CTATACT	605	0.0	9.76389	4
TTAGGAC	975	0.0	9.576811	3
CTAAGAC	480	0.0	9.527949	3
GTAGGAC	1290	0.0	9.232509	3
GTATAAG	435	0.0	9.221468	1
AGGACGT	1075	0.0	9.200206	5
CTAAAGT	995	0.0	9.192524	4
TAGGACG	1145	0.0	9.153218	4
AGCGCCG	105	4.5467104E-4	9.037258	10
TAGGACC	675	0.0	9.033641	4
TGCGTTG	725	0.0	9.031556	12
AGCACCG	190	1.0404619E-8	9.00931	5
GTATTAA	500	0.0	8.977757	1
GTCCTAC	1265	0.0	8.909052	1
>>END_MODULE