FastQCFastQC Report
Thu 26 May 2016
SRR1513187_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513187_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3400158
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACCG600.005785915511.1068915
ACTGCGC951.3781431E-510.9878758
TTAGGAC9400.010.3307283
GTCCTAA8550.010.136791
GTATTAG2159.094947E-129.7456191
GACGTGA7650.09.6775897
GGCGAGG6350.09.56354519
CGATACT1106.684016E-59.5201934
GTCCTAC12800.09.4497211
TAGGACG12450.09.4054924
CGTGAAA8300.09.3740939
AGGACGT12200.09.3641235
CTACACT5350.09.2532714
TTTAGGA11850.09.2402322
TAGGACC5700.09.1861514
GGACGTG12550.09.1778836
ACGTGAA8500.09.1546048
GTATTAA4700.09.1188461
ATTAGAC2304.0017767E-119.1065413
TACACCG1151.085911E-49.1062725
Produced by FastQC (version 0.11.5)