FastQCFastQC Report
Thu 26 May 2016
SRR1513186_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513186_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648560
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACT855.226799E-511.1970994
TAGGACC4550.010.877184
AGGACGT9850.010.8133685
TTAGGAC7550.010.7153073
GTCCTAA7300.010.7142581
CGGGACC803.7088204E-410.7072264
TAGGACG9800.010.5858284
GTAGGAC10900.010.4782163
ACCGTGC1002.4230085E-510.4410178
GGTATCA11500.010.2847791
TAGGACT5000.010.0885864
GGACGTG10300.09.9524136
CTAGGAC3350.09.943883
GTATTAG2850.09.7056431
TGGCGAG9100.09.70154418
GTATCAA21750.09.6917931
TTAGACA3150.09.6686054
GACGTGA5700.09.6583567
TGTAGGA13050.09.5597922
TTATACC2303.6379788E-129.5175344
Produced by FastQC (version 0.11.5)