##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513186_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2648560
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.66912246654786	31.0	30.0	34.0	25.0	34.0
2	29.61437196061256	31.0	30.0	34.0	24.0	34.0
3	29.767839882804243	31.0	30.0	34.0	25.0	34.0
4	32.940593001480046	37.0	35.0	37.0	25.0	37.0
5	33.0309617301477	37.0	35.0	37.0	26.0	37.0
6	33.35260292385296	37.0	35.0	37.0	28.0	37.0
7	33.313400866886155	37.0	35.0	37.0	27.0	37.0
8	33.241133672637204	37.0	35.0	37.0	27.0	37.0
9	34.51221644969342	39.0	35.0	39.0	26.0	39.0
10	34.140640196937206	39.0	35.0	39.0	23.0	39.0
11	34.51722747455221	39.0	35.0	39.0	27.0	39.0
12	34.513440511070165	39.0	35.0	39.0	27.0	39.0
13	34.54432295284985	39.0	35.0	39.0	27.0	39.0
14	34.88929682544477	40.0	35.0	41.0	18.0	41.0
15	35.27213882260549	40.0	35.0	41.0	25.0	41.0
16	35.090470293291446	40.0	35.0	41.0	23.0	41.0
17	34.737231552239706	40.0	34.0	41.0	17.0	41.0
18	34.4797663636089	39.0	34.0	41.0	17.0	41.0
19	35.09544922523938	40.0	35.0	41.0	24.0	41.0
20	35.29641767602018	40.0	36.0	41.0	25.0	41.0
21	35.34348740447639	40.0	36.0	41.0	25.0	41.0
22	35.05883649983387	40.0	36.0	41.0	22.0	41.0
23	34.59346324040233	40.0	34.0	41.0	18.0	41.0
24	34.830630229256656	40.0	35.0	41.0	19.0	41.0
25	34.45171225118555	40.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.7491993777215384
1101	2	1.0122720012182853
1101	3	0.9019668918009742
1101	4	1.2525050008257637
1101	5	1.145734605020202
1101	6	0.8220066665003714
1101	7	0.8226534277081896
1101	8	0.8294890552468459
1101	9	1.0955444288363623
1101	10	1.4751493588005857
1101	11	1.0932805434467525
1101	12	0.9498903299306534
1101	13	0.7925313073453353
1101	14	1.7801599917316224
1101	15	1.4357985450584962
1101	16	1.5006058051900624
1101	17	1.9168474326131317
1101	18	1.880614104867469
1101	19	1.4436714871869896
1101	20	1.041504668189333
1101	21	1.0083943161207074
1101	22	1.297399094276635
1101	23	1.639232158891481
1101	24	1.248987952299096
1101	25	1.483978482240225
1102	1	0.7671953905087285
1102	2	0.8576929933983841
1102	3	0.7227568535757207
1102	4	1.053991188041877
1102	5	1.071409685702207
1102	6	0.6719941350181955
1102	7	0.7533860833536465
1102	8	0.7607358826862196
1102	9	0.9469569300905647
1102	10	1.2251522164865563
1102	11	0.8639535729452703
1102	12	0.7698487050885134
1102	13	0.6898196173775943
1102	14	1.7552934668344093
1102	15	1.2499035326307322
1102	16	1.4065175530735772
1102	17	1.6930437340477766
1102	18	1.9170776486266874
1102	19	1.2721218466415465
1102	20	1.0441425240188664
1102	21	0.9284594417255363
1102	22	1.2098851074914236
1102	23	1.3900940158115915
1102	24	1.1728598331348579
1102	25	1.4476907390881948
1103	1	0.47459982904362263
1103	2	0.7734899055091695
1103	3	0.59281958005052
1103	4	1.0195751220839213
1103	5	0.8896841722721831
1103	6	0.6588459703332816
1103	7	0.4735691014477865
1103	8	0.5784369868721058
1103	9	0.8948059017875281
1103	10	1.2318734479782876
1103	11	0.8033255093203664
1103	12	0.695797104751982
1103	13	0.7344166582288523
1103	14	1.5467726919572868
1103	15	1.161487310243487
1103	16	1.1823433397738654
1103	17	1.561287133432053
1103	18	1.7473798332203643
1103	19	1.1795676720183508
1103	20	0.838035476188935
1103	21	0.8925143212216469
1103	22	1.0283523597083573
1103	23	1.4754005785434003
1103	24	1.1255203127579563
1103	25	1.3859684023440195
1104	1	0.5322944906867342
1104	2	0.8057110945223158
1104	3	0.5642504810404532
1104	4	1.0694568916713578
1104	5	0.8908520422819493
1104	6	0.7011355501535803
1104	7	0.6355378341520677
1104	8	0.6991906794966241
1104	9	1.0146607990864638
1104	10	1.0792226872589907
1104	11	0.7490092585649037
1104	12	0.7137265530697263
1104	13	0.6302258290763234
1104	14	1.6488737818830685
1104	15	1.1842017455906984
1104	16	1.337361088562929
1104	17	1.7212708389189757
1104	18	1.8619622428651255
1104	19	1.2232463414887889
1104	20	0.9307321446397765
1104	21	0.9229678225191122
1104	22	1.1837760744745012
1104	23	1.5060816111734994
1104	24	1.237808732675461
1104	25	1.5090812192905645
1105	1	0.29311538520141056
1105	2	0.5531274398270085
1105	3	0.4604574368349077
1105	4	0.7133710337517414
1105	5	0.6619451286386635
1105	6	0.39992068660058777
1105	7	0.3160036242932662
1105	8	0.4237614657249722
1105	9	0.6409456150890378
1105	10	0.9562592752621697
1105	11	0.3639227115599155
1105	12	0.418676791216555
1105	13	0.38047569264074355
1105	14	1.1149035552743172
1105	15	0.7274719241899135
1105	16	0.9322071370830969
1105	17	1.2144803252377088
1105	18	0.9357904601324805
1105	19	0.7172540824292497
1105	20	0.5630585587969179
1105	21	0.4570426480093346
1105	22	0.6387512937841819
1105	23	1.0546708558324696
1105	24	0.7409799380887634
1105	25	0.9296599222104192
1106	1	0.4285824239315339
1106	2	0.708710098904966
1106	3	0.626500047247653
1106	4	1.092878891284606
1106	5	0.8855974819515069
1106	6	0.5293548508846939
1106	7	0.4778398459947155
1106	8	0.5821234801679083
1106	9	0.7633022515693995
1106	10	0.6379622883893319
1106	11	0.5181442252688413
1106	12	0.5595306158674447
1106	13	0.43829192282305485
1106	14	1.4065622730942806
1106	15	0.9409703763445378
1106	16	1.0996228487656623
1106	17	1.40032237724202
1106	18	1.4761971758611523
1106	19	0.9993285533858582
1106	20	0.7469193248662975
1106	21	0.5968270721541558
1106	22	0.7949087758652098
1106	23	1.2969402173696167
1106	24	0.9465255234324346
1106	25	1.2462207974283004
1107	1	0.4341609419711254
1107	2	0.6896477818582945
1107	3	0.6221011514312877
1107	4	0.9218918954328927
1107	5	0.8246017333227442
1107	6	0.5091891544075651
1107	7	0.5542096404592911
1107	8	0.6304639696184395
1107	9	0.9267421509163825
1107	10	1.2315331618878673
1107	11	0.7062709142621131
1107	12	0.6975809863473685
1107	13	0.6103052197750003
1107	14	1.4387781429689284
1107	15	0.8452366880331184
1107	16	1.0047886165245785
1107	17	1.4970292578041864
1107	18	1.646423519302246
1107	19	1.091991014896955
1107	20	0.8095289663105447
1107	21	0.7308904145619479
1107	22	0.9643839711342324
1107	23	1.2148036478326105
1107	24	1.0654479871754603
1107	25	1.3805467597024261
1108	1	0.4828303321354994
1108	2	0.7119049815756284
1108	3	0.5009880011053376
1108	4	0.9158349947189279
1108	5	0.8697084890777731
1108	6	0.5189239915531019
1108	7	0.472601402432133
1108	8	0.5636772018911813
1108	9	0.771163443446838
1108	10	1.0000877045660062
1108	11	0.5656770911248898
1108	12	0.445769240772222
1108	13	0.4354310420642449
1108	14	1.419695449786829
1108	15	0.9411696385519264
1108	16	1.0648717601247526
1108	17	1.437714081967819
1108	18	1.648247709885439
1108	19	0.9792242818864523
1108	20	0.8141114398089329
1108	21	0.7939189201721533
1108	22	1.076246219203611
1108	23	1.3395818595451559
1108	24	1.002793952509066
1108	25	1.2089487336188398
1109	1	0.42680369439828425
1109	2	0.5838369453308374
1109	3	0.5390666538395976
1109	4	0.7790880178869273
1109	5	0.8580482293983351
1109	6	0.5019225776033593
1109	7	0.4760934521951512
1109	8	0.49427204701331817
1109	9	0.7979618783452906
1109	10	1.131708388419817
1109	11	0.6013427471242565
1109	12	0.5987512718495864
1109	13	0.4940840611185493
1109	14	1.4882332485502943
1109	15	0.8995796333465194
1109	16	1.0915447801600138
1109	17	1.3955513242753455
1109	18	1.5978547731998987
1109	19	1.0125358513284723
1109	20	0.8859030106432755
1109	21	0.7542268036407549
1109	22	1.0690126084421934
1109	23	1.3948952463951585
1109	24	1.1459949954881807
1109	25	1.4085126295382153
1110	1	0.4647528817815072
1110	2	0.7143790519923705
1110	3	0.5743787225621624
1110	4	1.016964597377509
1110	5	0.8123120337722298
1110	6	0.5380329028759618
1110	7	0.44131545486066415
1110	8	0.5426722531121655
1110	9	0.5998573208285478
1110	10	0.9834579159306784
1110	11	0.6675598544043027
1110	12	0.49049247365542215
1110	13	0.37971135566241543
1110	14	1.2240609946614711
1110	15	0.870837987287949
1110	16	1.068954367198593
1110	17	1.3573598954488304
1110	18	1.1190559551488235
1110	19	0.8030901561159709
1110	20	0.6271580622701762
1110	21	0.44912037122687565
1110	22	0.7702172540565186
1110	23	-0.5019954147239005
1110	24	0.22782624183761868
1110	25	0.686413691334522
1111	1	0.39132904201112595
1111	2	0.7496881606559782
1111	3	0.6938668813027142
1111	4	1.0625662848152189
1111	5	0.916458174317313
1111	6	0.5949720894205583
1111	7	0.5509492376852094
1111	8	0.7524103117870311
1111	9	0.8141754857205825
1111	10	1.0435573730628462
1111	11	0.7042350231864489
1111	12	0.6312985961297741
1111	13	0.47828738012913874
1111	14	1.5039273460324978
1111	15	0.9912691901418214
1111	16	1.1625391595056769
1111	17	1.400793105669571
1111	18	1.7433306207867503
1111	19	1.095240293451596
1111	20	0.8488419048530957
1111	21	0.6501444731407062
1111	22	1.0336372165855252
1111	23	1.4288988920222039
1111	24	1.101643707539921
1111	25	1.174605886200112
1112	1	0.15189318771042437
1112	2	0.18708850776630825
1112	3	0.10359705254388629
1112	4	0.5152788119356657
1112	5	0.3424803556835272
1112	6	0.19766317592210214
1112	7	0.08029938671517556
1112	8	0.17763661603339642
1112	9	0.459265606424033
1112	10	0.6744504166223493
1112	11	0.09927895934981734
1112	12	0.09812194438610788
1112	13	-0.029023910176618983
1112	14	0.6734523132210057
1112	15	0.3638404637953414
1112	16	0.1779752834545505
1112	17	0.5551369593569433
1112	18	0.7526482325698822
1112	19	0.2279573879903154
1112	20	0.10220614154182783
1112	21	-0.008301981002666992
1112	22	0.2780962214828264
1112	23	0.6506568074130215
1112	24	0.34578023386353607
1112	25	0.5680836420767648
1113	1	0.14746975201584078
1113	2	0.3024721457212358
1113	3	0.20057229132676113
1113	4	0.6670140921871379
1113	5	0.5569718013011169
1113	6	0.2677361972275989
1113	7	0.1718104372885776
1113	8	0.3023523507056325
1113	9	0.2862238753832713
1113	10	0.6351703219048446
1113	11	0.2207218574964429
1113	12	0.1747787585006293
1113	13	-0.05624533609306326
1113	14	0.6606297054733119
1113	15	0.4582724853419151
1113	16	0.5461117973136353
1113	17	0.8585158424361694
1113	18	0.8621862472311435
1113	19	0.6650232116680286
1113	20	0.3282916936786151
1113	21	0.22236067339125754
1113	22	0.46041934165031506
1113	23	0.4998869901859848
1113	24	0.40124804404376846
1113	25	0.7809113562094581
1114	1	-0.6307277850686326
1114	2	-0.3489587856545455
1114	3	-0.32612625251893945
1114	4	-0.0830068191273341
1114	5	-3.2903627298388756
1114	6	-0.9465002503003959
1114	7	-0.6273007548534508
1114	8	-0.32942927480875994
1114	9	0.05090475763748259
1114	10	0.20698242435119596
1114	11	-0.20759945362758003
1114	12	-0.33701220473179916
1114	13	-0.328954931214291
1114	14	-6.044142447431206
1114	15	-2.3288128223500593
1114	16	-1.0527694627609065
1114	17	-0.03149076664790584
1114	18	-6.764287538480545
1114	19	-2.4414466073613283
1114	20	-1.2694222284524557
1114	21	-0.866914070483638
1114	22	-0.591750969457074
1114	23	-0.1857622429939596
1114	24	-0.32411217567594264
1114	25	-0.1756929542930763
1115	1	-0.2585834981432633
1115	2	0.06002169789789136
1115	3	0.05021254176893564
1115	4	-2.683404504582704
1115	5	-0.5747046808966161
1115	6	-0.2199654579159116
1115	7	-0.1584318411493726
1115	8	0.14931938772880216
1115	9	0.375731404345494
1115	10	0.08507755216492541
1115	11	-0.04922560691774436
1115	12	-0.05456091141642361
1115	13	0.009212532652696837
1115	14	-5.3177565542281044
1115	15	-1.8489827979358253
1115	16	-0.5928303452371679
1115	17	0.2858104403803239
1115	18	0.527257996825746
1115	19	0.2075302736618596
1115	20	0.20149059791199875
1115	21	0.11971274588366754
1115	22	-0.942236992601849
1115	23	-0.10537477810706264
1115	24	0.09088351314996856
1115	25	0.4963227579881391
1116	1	0.6252572835557793
1116	2	0.8744429327657883
1116	3	0.7521981839404646
1116	4	0.9089410503424418
1116	5	0.43282761512999457
1116	6	0.6306195424072882
1116	7	0.7251838118293676
1116	8	0.7106371297408955
1116	9	0.7472073382910409
1116	10	1.1417211727148384
1116	11	0.9527391684104813
1116	12	0.48487579354955557
1116	13	0.6486964066293055
1116	14	1.623157187179757
1116	15	1.330509775988439
1116	16	1.2696154922733953
1116	17	1.8615131017515836
1116	18	1.516373631886779
1116	19	1.4097625629500357
1116	20	0.9647856662213314
1116	21	0.9448587151965455
1116	22	1.0439161336782163
1116	23	0.2608244335480592
1116	24	0.5941156448651839
1116	25	1.3962208928146964
1201	1	0.44009566007201784
1201	2	0.5360298864006374
1201	3	0.5376455476878519
1201	4	0.8490774695027383
1201	5	0.8549600989926844
1201	6	0.47586342968718043
1201	7	0.36224433573434567
1201	8	0.45653107758465694
1201	9	0.606267373468313
1201	10	1.0292962296806039
1201	11	0.5761636328195223
1201	12	0.579799657883548
1201	13	0.4035031197978256
1201	14	1.2847037307342788
1201	15	0.9274097078915986
1201	16	0.9668977240342826
1201	17	1.3372483858571371
1201	18	0.6861404789005121
1201	19	0.6434632815027541
1201	20	0.7056713995449684
1201	21	0.5535336467007284
1201	22	0.7290495725950805
1201	23	1.1027287790811826
1201	24	0.7630056224185537
1201	25	1.0454634453906237
1202	1	0.5947784186895149
1202	2	0.801800826923369
1202	3	0.6868691222306609
1202	4	1.0830481770795828
1202	5	0.9940062585337301
1202	6	0.5871298083573109
1202	7	0.5330149162142916
1202	8	0.6423481570378513
1202	9	0.8527441325137488
1202	10	1.1885225175223013
1202	11	0.7147848853356464
1202	12	0.6055992291319541
1202	13	0.596168927467339
1202	14	1.5369302265835643
1202	15	1.0880397257148715
1202	16	1.096181866973886
1202	17	1.5254540832091337
1202	18	1.6868099374706844
1202	19	1.1730911535121464
1202	20	0.8442059436842797
1202	21	0.8470681263355928
1202	22	1.046270138917457
1202	23	1.2913373851265817
1202	24	0.9138741376200983
1202	25	1.2737763671334648
1203	1	0.23756185192389268
1203	2	0.4502379260023517
1203	3	0.33206362649907817
1203	4	0.5686157496170168
1203	5	0.5246188125064108
1203	6	0.2325955350930755
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2211	12	-0.34332828134554205
2211	13	-0.3532114721896349
2211	14	0.11857223298105879
2211	15	-0.1891476620782342
2211	16	-6.643964049283952
2211	17	-1.9202898407173379
2211	18	-0.528370317238462
2211	19	-0.638854579166285
2211	20	-0.6954771344380433
2211	21	-0.5252517611776994
2211	22	-0.485622747870039
2211	23	-0.07204976870730206
2211	24	-0.5516629697368103
2211	25	-7.466914730747064
2212	1	-1.3018820230156898
2212	2	-3.7616404298717043
2212	3	-1.6860152617260482
2212	4	-3.331513533530824
2212	5	-1.1039651143185765
2212	6	-0.9313116072582659
2212	7	-0.8422896620079001
2212	8	-0.690919014166937
2212	9	-0.8257299012230774
2212	10	-0.22825922641896312
2212	11	-0.7387101940806247
2212	12	-0.8394075614258725
2212	13	-1.0783275814381525
2212	14	-6.782918678860572
2212	15	-5.019619174724973
2212	16	-2.67611787959882
2212	17	-7.302158528510105
2212	18	-2.9627939819786917
2212	19	-1.835133184560874
2212	20	-1.8724140021932456
2212	21	-1.4055094886178807
2212	22	-1.2257283719898453
2212	23	-0.7713112082025475
2212	24	-1.1137569150699491
2212	25	-0.9142899803816107
2213	1	-0.18921331213818604
2213	2	-0.04260951073752395
2213	3	-0.16403615775668356
2213	4	0.28586994086047923
2213	5	0.07758138716313567
2213	6	-0.19549513080580994
2213	7	-0.1991348534955364
2213	8	-0.16835085050242782
2213	9	0.06715173595085844
2213	10	-3.9304654791658926
2213	11	-1.500291890419419
2213	12	-0.9282484385772918
2213	13	-0.6264086839525476
2213	14	0.21772761227494186
2213	15	-0.31349248568828614
2213	16	-0.2243104705013792
2213	17	0.31522467046521285
2213	18	0.43880052230476707
2213	19	-0.18277727903362262
2213	20	-0.48641823269284856
2213	21	-0.5755452546876967
2213	22	-0.7200370935821354
2213	23	-0.9561593964363695
2213	24	-0.4499166589815644
2213	25	-0.0981563106515253
2214	1	-0.4878663023216667
2214	2	-0.2538337040407477
2214	3	-0.15969644257260995
2214	4	0.27193941146049383
2214	5	-3.472267718922403
2214	6	-1.0177913079799836
2214	7	-0.6424009935491242
2214	8	-0.26811943425961005
2214	9	-0.23614997248742498
2214	10	-3.74185664557292
2214	11	-1.2810521149513079
2214	12	-0.6913340206514107
2214	13	-0.3681698371595914
2214	14	0.4406450930020327
2214	15	0.10978461969457243
2214	16	0.11731186972553331
2214	17	0.47473229684942453
2214	18	0.97397723885733
2214	19	0.1535023354840419
2214	20	-0.26524125671328136
2214	21	-0.33037162529353736
2214	22	0.001759152355127469
2214	23	0.15154216186054015
2214	24	-0.3982353065786981
2214	25	-0.06141613851745831
2215	1	-1.5464161913437842
2215	2	-4.217754157136646
2215	3	-2.1906562099017393
2215	4	-1.0570503071404218
2215	5	-1.1329557817843572
2215	6	-1.2431315125874747
2215	7	-1.360868988548745
2215	8	-1.5243817757653346
2215	9	-7.427362055044764
2215	10	-3.022322242429823
2215	11	-2.1396059583576914
2215	12	-2.458496813722313
2215	13	-1.824524787793571
2215	14	-7.071507240084685
2215	15	-3.6228532097311508
2215	16	-8.732517453947196
2215	17	-3.7000248274202896
2215	18	-1.9652733415442327
2215	19	-2.4670891638654027
2215	20	-2.108533600515102
2215	21	-2.217654197939403
2215	22	-8.63274501888247
2215	23	-9.83340668626559
2215	24	-5.680911531800017
2215	25	-3.9997856565928167
2216	1	-0.9119963410951115
2216	2	-0.8919198673623647
2216	3	-0.6627538215320641
2216	4	-5.976440917623016
2216	5	-2.5025797638579235
2216	6	-1.5383624247588692
2216	7	-0.9815833133014351
2216	8	-7.183521798719916
2216	9	-2.6745916555257168
2216	10	-0.9064884991932232
2216	11	-0.8545190588659679
2216	12	-1.0332915888220526
2216	13	-0.8271894322708064
2216	14	-0.1446703269295213
2216	15	-0.6433644957343176
2216	16	-0.41900922855505485
2216	17	0.04569605578460312
2216	18	-6.420331274538714
2216	19	-3.2321421780207267
2216	20	-2.5067666441834646
2216	21	-1.6137401683170651
2216	22	-0.9611234415747134
2216	23	-0.42426639004216327
2216	24	-0.9093063506227779
2216	25	-0.3297703535376755
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	111454.0
3	5.0
4	985.0
5	5536.0
6	6499.0
7	5954.0
8	5598.0
9	5306.0
10	4832.0
11	4746.0
12	4537.0
13	4589.0
14	4509.0
15	4695.0
16	4750.0
17	5026.0
18	5837.0
19	7023.0
20	9155.0
21	11602.0
22	13853.0
23	16664.0
24	19527.0
25	22519.0
26	25314.0
27	28718.0
28	32150.0
29	38469.0
30	46573.0
31	56085.0
32	71391.0
33	94988.0
34	126712.0
35	172600.0
36	250907.0
37	374217.0
38	1045235.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06134380942152	18.489103123614917	12.948464665136802	25.50108840182676
2	14.840456166028815	19.65329686588298	40.89147477487227	24.614772193215934
3	18.530350107827655	23.765714854409016	29.626665425485015	28.077269612278315
4	11.791645569332625	16.4542542261258	38.38250448109269	33.37159572344889
5	13.286155490119064	37.36607781392677	35.86870725595834	13.479059439995822
6	32.74386778469887	36.474384512698705	17.774974934906282	13.006772767696145
7	28.639730705847345	31.295702441139106	21.738199499416186	18.326367353597362
8	25.521288258047363	35.8585372245717	20.348037821791674	18.272136695589257
9	26.21666569741754	15.100045391069363	19.97648878553588	38.70680012597721
10	14.253539338593358	28.48264535466648	35.06974826124999	22.194067045490165
11	34.78074513309293	22.30852514523022	24.01008747234272	18.90064224933413
12	23.048883101423485	24.44936832198584	31.23089972762993	21.27084884896075
13	28.595421046628704	21.470217013475818	25.714899688577997	24.21946225131748
14	20.88669883765985	21.032510629041358	28.29602248394409	29.784768049354703
15	23.528785195839657	29.478158786948455	23.370256607057602	23.62279941015429
16	22.941239336815944	27.711646430738824	26.641636578068155	22.705477654377074
17	22.102092347323236	27.7595320877581	27.687010978260663	22.451364586658006
18	22.01012487649826	27.267144458510966	28.57735816561772	22.14537249937305
19	23.415128792442214	26.965347553595066	27.318197868849055	22.30132578511367
20	23.766131255641454	26.790819582976123	27.30928578712219	22.133763374260237
21	23.694446783993257	26.51031514414915	26.717664214609687	23.077573857247906
22	23.50029991735275	27.01295914929236	26.86194282985998	22.624798103494907
23	22.903984802534925	26.781377817894786	27.24194711856213	23.072690261008155
24	23.186039141203807	26.613623683416264	27.213331988560363	22.987005186819562
25	23.15868551268246	26.606293243814385	27.267940836211064	22.967080407292098
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	164.0
1	164.0
2	300.5
3	437.0
4	437.0
5	437.0
6	865.0
7	1293.0
8	1293.0
9	1293.0
10	2299.5
11	3306.0
12	3306.0
13	3306.0
14	6776.0
15	10246.0
16	10246.0
17	10246.0
18	19207.5
19	28169.0
20	28169.0
21	28169.0
22	44044.0
23	59919.0
24	59919.0
25	59919.0
26	84234.5
27	108550.0
28	108550.0
29	108550.0
30	139747.5
31	170945.0
32	170945.0
33	170945.0
34	201244.5
35	231544.0
36	231544.0
37	231544.0
38	259132.5
39	286721.0
40	286721.0
41	286721.0
42	307765.5
43	328810.0
44	328810.0
45	328810.0
46	340321.0
47	351832.0
48	351832.0
49	351832.0
50	344519.0
51	337206.0
52	337206.0
53	337206.0
54	307269.0
55	277332.0
56	277332.0
57	277332.0
58	239503.5
59	201675.0
60	201675.0
61	201675.0
62	164844.5
63	128014.0
64	128014.0
65	128014.0
66	98918.0
67	69822.0
68	69822.0
69	69822.0
70	51360.5
71	32899.0
72	32899.0
73	32899.0
74	22950.0
75	13001.0
76	13001.0
77	13001.0
78	8839.0
79	4677.0
80	4677.0
81	4677.0
82	3062.5
83	1448.0
84	1448.0
85	1448.0
86	920.5
87	393.0
88	393.0
89	393.0
90	255.5
91	118.0
92	118.0
93	118.0
94	71.5
95	25.0
96	25.0
97	25.0
98	19.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16295647446159423
2	0.22042166309239739
3	0.22340441598453498
4	0.30284380946627604
5	0.21876038300057388
6	0.017179146404083728
7	0.017065877306913946
8	0.01725465913553025
9	0.017443440964146555
10	0.020275168393391125
11	0.019746579273265473
12	0.026127405080496574
13	0.026655994200622226
14	0.02363548494276135
15	0.041078925906907905
16	0.020652732050623734
17	0.039832965838040295
18	0.03111124535596702
19	0.026240674177666356
20	0.028317274292445706
21	0.03213066723049506
22	0.04394840970187574
23	0.028317274292445706
24	0.048063853565711176
25	0.035000151025462896
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2648560.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.5024681314501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06407070520221	54.951480545310346
2	13.461333130076037	18.71191753759886
3	3.9468147373975304	8.229400965201283
4	1.5315353670274767	4.257819521560637
5	0.71178590229079	2.4735438495190545
6	0.3804688448574556	1.5866114258828963
7	0.24388670270250462	1.1865509447585691
8	0.14473723160159743	0.804767586145947
9	0.10312228734107926	0.6450528140609041
>10	0.3865776626878198	4.365168929811132
>50	0.016208349930626385	0.782047442023815
>100	0.00792454082905295	1.0345901989498314
>500	0.001041293680744125	0.47224948299625813
>1k	4.932443750180212E-4	0.4987987561805375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	3.775636572326094E-5
7	0.0	0.0	0.0	0.0	3.775636572326094E-5
8	0.0	0.0	0.0	0.0	3.775636572326094E-5
9	0.0	0.0	0.0	0.0	3.775636572326094E-5
10	0.0	0.0	0.0	0.0	3.775636572326094E-5
11	0.0	0.0	0.0	0.0	3.775636572326094E-5
12	0.0	0.0	0.0	0.0	3.775636572326094E-5
13	0.0	0.0	0.0	0.0	3.775636572326094E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGACT	85	5.226799E-5	11.197099	4
TAGGACC	455	0.0	10.87718	4
AGGACGT	985	0.0	10.813368	5
TTAGGAC	755	0.0	10.715307	3
GTCCTAA	730	0.0	10.714258	1
CGGGACC	80	3.7088204E-4	10.707226	4
TAGGACG	980	0.0	10.585828	4
GTAGGAC	1090	0.0	10.478216	3
ACCGTGC	100	2.4230085E-5	10.441017	8
GGTATCA	1150	0.0	10.284779	1
TAGGACT	500	0.0	10.088586	4
GGACGTG	1030	0.0	9.952413	6
CTAGGAC	335	0.0	9.94388	3
GTATTAG	285	0.0	9.705643	1
TGGCGAG	910	0.0	9.701544	18
GTATCAA	2175	0.0	9.691793	1
TTAGACA	315	0.0	9.668605	4
GACGTGA	570	0.0	9.658356	7
TGTAGGA	1305	0.0	9.559792	2
TTATACC	230	3.6379788E-12	9.517534	4
>>END_MODULE