FastQCFastQC Report
Thu 26 May 2016
SRR1513186_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513186_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648560
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33300.12572869785845892No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4200.012.2391244
ATACCGT951.0161511E-612.023726
TATACCG802.8091288E-511.8991485
CCGATCA1001.9583094E-611.3867369
GGTATCA11400.010.9434261
GGCGAGG5100.010.7907419
GTCCTAC9900.010.6776251
TGGACCG1252.180077E-710.6616375
AGGACGT11150.010.4157575
TGGCGAG9850.010.40687518
GGACGTG10850.010.3522196
GTGTTCG1106.133867E-610.3515789
TAGGACG11350.010.316094
GTAGGAC10450.010.2943973
AGTACCG856.4741506E-410.0792785
TCTATAC3000.09.8373753
TCGAACT2151.0913936E-119.70906819
GTATCAA22700.09.4813351
GTCCTAA6900.09.3852671
CTTAGAC2357.2759576E-129.3174863
Produced by FastQC (version 0.11.5)