##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513186_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2648560 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.44021128462259 31.0 31.0 34.0 27.0 34.0 2 30.57155397650044 31.0 31.0 34.0 27.0 34.0 3 30.659695457154076 31.0 31.0 34.0 27.0 34.0 4 34.03776995801492 37.0 35.0 37.0 30.0 37.0 5 33.77301590298124 37.0 35.0 37.0 28.0 37.0 6 33.69685300691697 37.0 35.0 37.0 29.0 37.0 7 33.55128371643459 37.0 35.0 37.0 28.0 37.0 8 33.51322907542212 37.0 35.0 37.0 28.0 37.0 9 35.0391129519437 39.0 35.0 39.0 28.0 39.0 10 34.86536231008548 39.0 35.0 39.0 27.0 39.0 11 34.88000271845833 39.0 35.0 39.0 27.0 39.0 12 34.81105279850183 39.0 35.0 39.0 27.0 39.0 13 34.77856080285136 39.0 35.0 39.0 27.0 39.0 14 35.91283150089105 40.0 36.0 41.0 27.0 41.0 15 35.88720285740176 40.0 36.0 41.0 27.0 41.0 16 35.84109893678074 40.0 36.0 41.0 27.0 41.0 17 35.765976983719455 40.0 36.0 41.0 27.0 41.0 18 35.71911227232911 40.0 36.0 41.0 27.0 41.0 19 35.799693795873985 40.0 36.0 41.0 27.0 41.0 20 35.75844798683058 40.0 36.0 41.0 27.0 41.0 21 35.704044084332615 40.0 36.0 41.0 27.0 41.0 22 35.6431589240946 40.0 36.0 41.0 27.0 41.0 23 35.55741346240976 40.0 36.0 41.0 26.0 41.0 24 35.49090977738847 40.0 36.0 41.0 26.0 41.0 25 35.43066345485849 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7895259727047375 1101 2 0.8065662022714761 1101 3 0.708240265878608 1101 4 0.936065219363833 1101 5 0.8961517192516624 1101 6 0.9367623673758345 1101 7 0.9253404874016624 1101 8 0.9604186101387953 1101 9 1.0191543755106522 1101 10 1.1996176975645838 1101 11 1.1314761082535725 1101 12 1.1394238215148604 1101 13 1.1288237312663867 1101 14 1.2726221704387655 1101 15 1.2258284384165634 1101 16 1.266748829191286 1101 17 1.2024987792623136 1101 18 1.196385006933106 1101 19 1.246733844914857 1101 20 1.3079237540379935 1101 21 1.2359955451023623 1101 22 1.328105606083085 1101 23 1.3814404075605182 1101 24 1.3880051173522432 1101 25 1.3447448488168163 1102 1 0.7942933337820399 1102 2 0.7753866257085633 1102 3 0.7053545322513486 1102 4 0.866661870325224 1102 5 0.825975419078631 1102 6 0.8063442476993359 1102 7 0.867901634472581 1102 8 0.9062012160181752 1102 9 0.9409866094463339 1102 10 1.0556644128979968 1102 11 1.097713712776084 1102 12 1.0755621606108434 1102 13 1.1142013755255533 1102 14 1.2321182820807195 1102 15 1.0708260697124174 1102 16 1.1984586017575865 1102 17 1.2589196609452245 1102 18 1.235121786960299 1102 19 1.197096262999814 1102 20 1.151332300254822 1102 21 1.2554062182842713 1102 22 1.1945516523369548 1102 23 1.2646948334810446 1102 24 1.3269459996617314 1102 25 1.312482103431556 1103 1 0.7249214894241689 1103 2 0.7847264271562899 1103 3 0.7494996430097736 1103 4 0.9015590400215174 1103 5 0.8457189712324293 1103 6 0.6903344098023325 1103 7 0.7676411211377143 1103 8 0.7759365493600114 1103 9 0.8885232453757581 1103 10 0.9812532133968972 1103 11 1.0083411385542504 1103 12 1.0088786310890967 1103 13 1.0079287385019597 1103 14 1.1089624695089526 1103 15 1.006987127475604 1103 16 1.0898821355414015 1103 17 1.2139620275615357 1103 18 1.171226850145814 1103 19 0.9815959809879047 1103 20 1.0471189148006985 1103 21 0.9910618295683449 1103 22 1.0055655609508776 1103 23 1.0059781350928318 1103 24 1.0555764611720733 1103 25 0.9921924394554864 1104 1 0.6093902565323503 1104 2 0.6798818205025015 1104 3 0.6777140766497283 1104 4 0.8285599214855637 1104 5 0.7723380470496153 1104 6 0.807861223888743 1104 7 0.8206843242573854 1104 8 0.9065511747098611 1104 9 0.8127450163062306 1104 10 0.9090366412314381 1104 11 0.8981639796994045 1104 12 0.8913373311515826 1104 13 0.8760080584698855 1104 14 1.0799362901614913 1104 15 1.0057994946261246 1104 16 1.043666941808148 1104 17 1.00987971812782 1104 18 0.9504269890444306 1104 19 1.0000836519248324 1104 20 1.064266178894215 1104 21 1.0583766483974273 1104 22 0.9344728838900593 1104 23 0.9914462914154427 1104 24 0.9226794347758513 1104 25 0.904671565602726 1105 1 0.41666261062210097 1105 2 0.5180590411305239 1105 3 0.39061154460891956 1105 4 0.4975020725911605 1105 5 0.5085939929299315 1105 6 0.41279072922848314 1105 7 0.5117406732432954 1105 8 0.5625933857079275 1105 9 0.575128350120842 1105 10 0.5190243905938203 1105 11 0.5140913251293711 1105 12 0.5640999901611039 1105 13 0.6561162803990044 1105 14 0.7187663241462587 1105 15 0.6457039782930138 1105 16 0.5187647215073241 1105 17 0.6570227144588614 1105 18 0.5888303177610084 1105 19 0.7027275210904307 1105 20 0.7334204361725583 1105 21 0.730554405419312 1105 22 0.7175472683407023 1105 23 0.650957612089023 1105 24 0.6940911997505737 1105 25 0.7822136975543756 1106 1 0.5197035235057719 1106 2 0.5825648327463782 1106 3 0.44197633939919356 1106 4 0.565195390892427 1106 5 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1107 18 0.8194120766132613 1107 19 0.8146581526264924 1107 20 0.8941954458066093 1107 21 0.8605009371159227 1107 22 0.9979023783855254 1107 23 0.9970143103892539 1107 24 1.0412833038230644 1107 25 1.0269241922624985 1108 1 0.45966071086590077 1108 2 0.4917945234004222 1108 3 0.5465530537555665 1108 4 0.6435018334930831 1108 5 0.5002649581159346 1108 6 0.5059661258066228 1108 7 0.5413993622958273 1108 8 0.6246880120567226 1108 9 0.7837326187199096 1108 10 0.8128863551232968 1108 11 0.7024553783275493 1108 12 0.6796011961263773 1108 13 0.7234715856717031 1108 14 0.7634123146309051 1108 15 0.727728430293034 1108 16 0.7548106020735545 1108 17 0.8275837058883369 1108 18 0.8556802964308332 1108 19 0.893662504026814 1108 20 0.9645384886092501 1108 21 0.8668611572093425 1108 22 0.6526254166209142 1108 23 0.7756945807485138 1108 24 0.8400482273424359 1108 25 0.8016770526466033 1109 1 0.3955703404384927 1109 2 0.36720139653574435 1109 3 0.395163443359106 1109 4 0.38805738863843686 1109 5 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0.49796505022420234 1110 18 0.5112160482666965 1110 19 0.48600477186672464 1110 20 0.5134083062690777 1110 21 0.44551166483492466 1110 22 0.590799449374309 1110 23 0.49691634108091876 1110 24 0.4573137192799095 1110 25 0.5450799303987779 1111 1 0.3300279475629573 1111 2 0.3583236354502688 1111 3 0.37640518616392526 1111 4 0.5164009329790034 1111 5 0.36907843851784605 1111 6 0.24137427513597487 1111 7 0.21212778188061776 1111 8 0.20424287290840937 1111 9 0.3073091136591799 1111 10 0.40530679294890604 1111 11 0.3715038565520885 1111 12 0.33550279106638214 1111 13 0.3444637988183601 1111 14 0.3865817368681306 1111 15 0.3307438558740259 1111 16 0.3587955486881569 1111 17 0.48263735414977305 1111 18 0.42801436134782733 1111 19 0.40077139749826074 1111 20 0.37137312962651947 1111 21 0.39929196436289516 1111 22 0.44421443212885237 1111 23 0.37976495799098586 1111 24 0.31988567172441407 1111 25 0.3732086950223348 1112 1 -0.4847037373645975 1112 2 -0.44762541562708336 1112 3 -0.3157071337402044 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2216 15 -2.9009306778785344 2216 16 -2.930369682286546 2216 17 -3.061142032952546 2216 18 -3.0363765016024473 2216 19 -3.0381625924818962 2216 20 -2.8991553375605363 2216 21 -3.3336329408139562 2216 22 -3.1402043759058174 2216 23 -3.18719672740896 2216 24 -3.229386939128716 2216 25 -3.3302891639108942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79856.0 3 1.0 4 1256.0 5 10294.0 6 11187.0 7 8628.0 8 6887.0 9 6139.0 10 5381.0 11 4833.0 12 4566.0 13 4284.0 14 4165.0 15 4107.0 16 4263.0 17 4401.0 18 5002.0 19 5988.0 20 7371.0 21 9525.0 22 11985.0 23 14367.0 24 16153.0 25 18372.0 26 20322.0 27 22626.0 28 25225.0 29 30482.0 30 38643.0 31 49320.0 32 63186.0 33 81798.0 34 108777.0 35 146767.0 36 210979.0 37 351507.0 38 1249917.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.61129929782713 19.61760459304197 14.29564643744579 27.475449671685105 2 14.109072674966328 20.41273517669852 38.663356971674396 26.81483517666076 3 16.900179991595106 23.931104035293004 29.915418675855417 29.253297297256474 4 11.66761877756512 17.313151854577363 36.56143607270679 34.45779329515073 5 13.209544040377935 37.74334106402418 35.555699136323895 13.491415759273995 6 32.81942539433258 37.0040989148728 17.27620080099486 12.900274889799759 7 28.250126799121865 31.445577967958627 21.76469632979328 18.539598903126226 8 25.564770893294497 36.30619860269568 19.947044114548444 18.181986389461386 9 25.95691241647052 14.992112415262268 19.510199489062728 39.54077567920449 10 14.577358217002631 28.95932163456528 34.369208951233986 22.094111197198096 11 34.894433201437764 22.271762769202887 23.75600326215 19.077800767209354 12 22.740885613314404 24.712296493188752 31.117852719968585 21.42896517352826 13 28.542113450327726 21.428625366236748 25.727527411121514 24.301733772314012 14 21.25047054243868 21.1719744357172 27.31022650600954 30.267328515834578 15 23.443946899447248 29.653170024466124 23.165946778626875 23.736936297459753 16 23.11686350318664 27.97010450962032 25.994653698613586 22.918378288579454 17 22.269825960397547 28.103509870401183 27.009823951347762 22.616840217853504 18 22.228222979408674 27.631019399398898 27.888250458680254 22.25250716251217 19 23.441719273869573 27.08264113329507 27.164572446914548 22.311067145920802 20 23.72696861690881 26.861917419276892 27.19477753949316 22.21633642432114 21 23.503293125138754 26.52423139018058 26.779503335412457 23.192972149268204 22 23.42836236610172 27.038400881384934 26.734273240656524 22.798963511856822 23 22.962219546426134 26.861015551460056 26.8630167348013 23.31374816731252 24 23.118071706889783 26.689823904310266 27.201649198054795 22.99045519074516 25 23.081985336177144 26.790492490444805 27.02114621573467 23.106375957643383 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 80.0 1 80.0 2 213.5 3 347.0 4 347.0 5 347.0 6 889.5 7 1432.0 8 1432.0 9 1432.0 10 2394.5 11 3357.0 12 3357.0 13 3357.0 14 6687.5 15 10018.0 16 10018.0 17 10018.0 18 18757.0 19 27496.0 20 27496.0 21 27496.0 22 43328.5 23 59161.0 24 59161.0 25 59161.0 26 83588.0 27 108015.0 28 108015.0 29 108015.0 30 138714.0 31 169413.0 32 169413.0 33 169413.0 34 198599.0 35 227785.0 36 227785.0 37 227785.0 38 255819.5 39 283854.0 40 283854.0 41 283854.0 42 304851.0 43 325848.0 44 325848.0 45 325848.0 46 338788.5 47 351729.0 48 351729.0 49 351729.0 50 345781.5 51 339834.0 52 339834.0 53 339834.0 54 310314.5 55 280795.0 56 280795.0 57 280795.0 58 243009.0 59 205223.0 60 205223.0 61 205223.0 62 167847.5 63 130472.0 64 130472.0 65 130472.0 66 100572.5 67 70673.0 68 70673.0 69 70673.0 70 52017.5 71 33362.0 72 33362.0 73 33362.0 74 23230.5 75 13099.0 76 13099.0 77 13099.0 78 8855.0 79 4611.0 80 4611.0 81 4611.0 82 3031.5 83 1452.0 84 1452.0 85 1452.0 86 930.0 87 408.0 88 408.0 89 408.0 90 245.0 91 82.0 92 82.0 93 82.0 94 47.5 95 13.0 96 13.0 97 13.0 98 7.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18534599933548798 2 0.006758389464463708 3 0.0038889056694958774 4 0.0022276255776723956 5 0.07422901501193102 6 0.3244404506599813 7 0.02533452140030809 8 0.01200652429999698 9 0.0035490983779865285 10 0.0028694837949678315 11 0.0 12 0.0 13 0.0 14 7.928836801884798E-4 15 0.0 16 0.0 17 0.02046395022200743 18 0.028166248829552664 19 0.0 20 0.0 21 4.530763886791313E-4 22 3.775636572326094E-5 23 0.004795058446854139 24 0.0 25 3.775636572326094E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2648560.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.03044775397984 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35281999628282 51.84994159383843 2 14.273151142716353 19.134714239130126 3 4.352328434491 8.752155712089294 4 1.7368871676455466 4.656972981816914 5 0.8072041925768038 2.7053629228656466 6 0.4419929572892985 1.7776191498724423 7 0.27278202549739533 1.2799290915829569 8 0.17145722027605798 0.9194283396605534 9 0.11393752909389707 0.6873555232031423 >10 0.44810484390400757 4.940322576133983 >50 0.01754442623868698 0.8173476281430669 >100 0.010095928777326102 1.288150030190407 >500 0.0010729523002459074 0.47842255765422803 >1k 6.211829106021334E-4 0.712277653818802 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3330 0.12572869785845892 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 420 0.0 12.239124 4 ATACCGT 95 1.0161511E-6 12.02372 6 TATACCG 80 2.8091288E-5 11.899148 5 CCGATCA 100 1.9583094E-6 11.386736 9 GGTATCA 1140 0.0 10.943426 1 GGCGAGG 510 0.0 10.79074 19 GTCCTAC 990 0.0 10.677625 1 TGGACCG 125 2.180077E-7 10.661637 5 AGGACGT 1115 0.0 10.415757 5 TGGCGAG 985 0.0 10.406875 18 GGACGTG 1085 0.0 10.352219 6 GTGTTCG 110 6.133867E-6 10.351578 9 TAGGACG 1135 0.0 10.31609 4 GTAGGAC 1045 0.0 10.294397 3 AGTACCG 85 6.4741506E-4 10.079278 5 TCTATAC 300 0.0 9.837375 3 TCGAACT 215 1.0913936E-11 9.709068 19 GTATCAA 2270 0.0 9.481335 1 GTCCTAA 690 0.0 9.385267 1 CTTAGAC 235 7.2759576E-12 9.317486 3 >>END_MODULE