FastQCFastQC Report
Thu 26 May 2016
SRR1513185_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513185_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507506
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT33450.1333994813970535No Hit
GTCCTAAAGTGTGTATTTCTCATTT31700.12642043528510002No Hit
CTGTAGGACGTGGAATATGGCAAGA28890.11521408124247759No Hit
CTTTAGGACGTGAAATATGGCGAGG26600.10608150090169274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACT1451.0004442E-1011.78029819
TAGGACC7100.011.1395834
TAGGACT4500.011.0113364
GCCGGTT700.001506037410.84492111
GTCCTAC18650.010.6546751
GGACGTG16800.010.5593416
GGCGAGG8400.010.39346419
GACGTGG9050.010.3778757
TGTAGGA20150.010.3643082
GTATTAG3050.010.33641051
AGGACGT18150.010.322965
AAAGGCG1951.4551915E-1110.2423455
ACGTGGA9400.010.1933138
TCCTACA19750.010.0913772
TAAGACT3400.010.0895734
GTCCTAA10900.010.0792221
CTAAGGT1803.7289283E-1010.0584334
GTAGGAC19500.010.0181113
TTAGGAC12850.09.9372983
CCTACAG20050.09.8858853
Produced by FastQC (version 0.11.5)