##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513185_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2507506
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.456537691235834	31.0	30.0	34.0	25.0	34.0
2	29.3341164487742	31.0	30.0	34.0	22.0	34.0
3	29.50976867054356	31.0	30.0	34.0	23.0	34.0
4	32.59813176917623	37.0	33.0	37.0	22.0	37.0
5	32.74273680701063	37.0	33.0	37.0	25.0	37.0
6	33.10127632795295	37.0	35.0	37.0	27.0	37.0
7	33.05552967769569	37.0	35.0	37.0	26.0	37.0
8	32.93624661316862	37.0	35.0	37.0	26.0	37.0
9	34.17547714741261	39.0	35.0	39.0	25.0	39.0
10	33.74749930807743	39.0	34.0	39.0	19.0	39.0
11	34.19880869676882	39.0	35.0	39.0	26.0	39.0
12	34.19145557378527	39.0	35.0	39.0	25.0	39.0
13	34.22886046932689	39.0	35.0	39.0	26.0	39.0
14	34.450251764103456	39.0	34.0	41.0	17.0	41.0
15	34.88673765885306	40.0	34.0	41.0	22.0	41.0
16	34.73173703273292	39.0	34.0	41.0	18.0	41.0
17	34.347809337245856	39.0	34.0	41.0	17.0	41.0
18	34.074541795712555	39.0	33.0	41.0	17.0	41.0
19	34.707632205067505	39.0	34.0	41.0	19.0	41.0
20	34.91919500890526	40.0	35.0	41.0	23.0	41.0
21	34.962487427746936	40.0	35.0	41.0	23.0	41.0
22	34.649016193779794	40.0	34.0	41.0	18.0	41.0
23	34.183915811168546	39.0	34.0	41.0	11.0	41.0
24	34.43251102888687	39.0	34.0	41.0	17.0	41.0
25	34.07184150307118	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	1.0928623942245288
1101	2	1.290034087293268
1101	3	1.1673017916213873
1101	4	1.561918057197019
1101	5	1.4280345301969746
1101	6	1.071435291499867
1101	7	1.1156649128350153
1101	8	1.2393646870967245
1101	9	1.605038294767354
1101	10	1.9329659222166242
1101	11	1.5205550960719734
1101	12	1.4907675724138656
1101	13	1.3860143013733932
1101	14	2.431562027019595
1101	15	2.0326762523750617
1101	16	2.042547127791657
1101	17	2.3559901368809903
1101	18	2.395966343776756
1101	19	2.063176640112765
1101	20	1.740478907047141
1101	21	1.6352976114522377
1101	22	2.0493113110806007
1101	23	2.2238374622249992
1101	24	1.9550975181360144
1101	25	2.1949911083064038
1102	1	0.6978864973281702
1102	2	0.8473968169536121
1102	3	0.7420744792235396
1102	4	0.9680033389706608
1102	5	1.0015025540524576
1102	6	0.6650508751352149
1102	7	0.6648188962958912
1102	8	0.6816219317741314
1102	9	0.9605987625037358
1102	10	1.297300118953899
1102	11	0.9003050939500525
1102	12	0.7311262387001847
1102	13	0.7811032686357606
1102	14	1.729428488112724
1102	15	1.1850116314799948
1102	16	1.3186550716122127
1102	17	1.5744851127409873
1102	18	2.005033524328063
1102	19	1.2636712122554599
1102	20	1.04040462158801
1102	21	0.911958138712933
1102	22	1.2644770584354958
1102	23	1.4321773611923447
1102	24	1.2541754263490006
1102	25	1.5461797679096208
1103	1	0.7571501812209327
1103	2	1.0247519173428365
1103	3	0.8598278929710155
1103	4	1.2834032580426893
1103	5	1.13561176828037
1103	6	0.8227769275200245
1103	7	0.8318147342857998
1103	8	0.8505365977214439
1103	9	1.2062681524609573
1103	10	1.4772604899380113
1103	11	1.0841480808345878
1103	12	0.9988396701747462
1103	13	0.9654681124961542
1103	14	1.7691506354016369
1103	15	1.454306691019866
1103	16	1.4874918604415868
1103	17	1.7995018121642161
1103	18	2.091735645154813
1103	19	1.5727221390734485
1103	20	1.2425591683271264
1103	21	1.2880708983568496
1103	22	1.518453608591571
1103	23	1.7710608441782085
1103	24	1.4144088007886069
1103	25	1.6431737111572744
1104	1	0.6409920254944339
1104	2	0.8304841713664572
1104	3	0.688130618755757
1104	4	1.067365482202753
1104	5	1.0256841259786214
1104	6	0.7888317892583387
1104	7	0.7038915317223058
1104	8	0.7147785375029514
1104	9	0.9794266819144468
1104	10	1.217166833261615
1104	11	0.835235955123899
1104	12	0.6786938387879076
1104	13	0.8104864701044363
1104	14	1.6388972623425104
1104	15	1.3684656236140498
1104	16	1.409444125469392
1104	17	1.7973507191857792
1104	18	1.8011784804969295
1104	19	1.2093001061370288
1104	20	1.001320557484199
1104	21	0.9702342585777615
1104	22	1.3191708270246139
1104	23	1.6279089155046904
1104	24	1.357190289883043
1104	25	1.6604030013182154
1105	1	0.7053996329186631
1105	2	0.8183931898634178
1105	3	0.7767104357156285
1105	4	1.007930024379796
1105	5	0.989395915167961
1105	6	0.776644898629371
1105	7	0.6165274353778116
1105	8	0.7638876145207334
1105	9	0.9602219572768291
1105	10	1.402209391611045
1105	11	0.8611811052814033
1105	12	0.816283289773537
1105	13	0.7860469589370709
1105	14	1.5909374692574332
1105	15	1.2331472470155944
1105	16	1.5460168125010227
1105	17	1.7047072389213653
1105	18	1.5195400246833302
1105	19	1.2688583304682624
1105	20	1.0260983303145679
1105	21	0.9944731168280327
1105	22	1.1018068932379563
1105	23	1.5162540130782673
1105	24	1.3331959044895072
1105	25	1.5584091940651703
1106	1	0.4754659354482911
1106	2	0.6610243695683273
1106	3	0.6417094552393188
1106	4	1.0297215116123226
1106	5	0.8709924097383563
1106	6	0.5557299781526339
1106	7	0.5903083125307589
1106	8	0.611520549313127
1106	9	0.8245713501840441
1106	10	0.7685743786275836
1106	11	0.7304343452147748
1106	12	0.6778610401491534
1106	13	0.571809745080877
1106	14	1.6068189523517233
1106	15	1.0707697629387383
1106	16	1.2448000448906527
1106	17	1.5057902290879923
1106	18	1.5653071933120017
1106	19	1.1657613512424945
1106	20	0.9619003704031357
1106	21	0.8839607359593913
1106	22	1.0254837561603907
1106	23	1.3424101900441272
1106	24	0.8789411606938984
1106	25	1.2333667823586296
1107	1	0.5566714522004297
1107	2	0.7559415567122869
1107	3	0.7246936533998287
1107	4	0.9602767542921526
1107	5	0.8066838202553797
1107	6	0.5123062185023528
1107	7	0.5312423824031214
1107	8	0.6137263572786082
1107	9	0.830954765795731
1107	10	1.00898976654274
1107	11	0.5988449606715776
1107	12	0.5887023135856495
1107	13	0.5466683946564785
1107	14	1.3540801065532122
1107	15	0.8732402159795924
1107	16	1.1464596332845503
1107	17	1.4544852779286828
1107	18	1.603530890214202
1107	19	1.0290135448653785
1107	20	0.8478304106637609
1107	21	0.6620507068203096
1107	22	0.9746599282190118
1107	23	1.0911276157563918
1107	24	0.9334999738431975
1107	25	1.2890213659242349
1108	1	0.7048890675290274
1108	2	0.9330942554091237
1108	3	0.7526614720409874
1108	4	1.217816401923173
1108	5	1.050496002760248
1108	6	0.7366075240877876
1108	7	0.7377085423957794
1108	8	0.7152333317805386
1108	9	1.0058623503833033
1108	10	1.1802549423492579
1108	11	0.8265967286098288
1108	12	0.5549446266490605
1108	13	0.580309595533592
1108	14	1.5187971785940562
1108	15	1.1735950814785525
1108	16	1.195790268876074
1108	17	1.5881304334133617
1108	18	1.7028254366489577
1108	19	1.1202128482087943
1108	20	0.981762745983275
1108	21	1.053010339423814
1108	22	1.2365234146279391
1108	23	1.490980407507564
1108	24	1.0999833027511343
1108	25	1.5013866167068812
1109	1	0.5078945971196163
1109	2	0.7586875597403662
1109	3	0.7229241131452788
1109	4	0.866470939000564
1109	5	0.9773399130938145
1109	6	0.6797980068641536
1109	7	0.7441435579122597
1109	8	0.6360780375278523
1109	9	0.9224365122318545
1109	10	1.3321828664121114
1109	11	0.8566877764686538
1109	12	0.6228148707152812
1109	13	0.697975320956175
1109	14	1.6572584441083578
1109	15	1.15988185541989
1109	16	1.3236020222096414
1109	17	1.6236458320886342
1109	18	1.6960402939240709
1109	19	1.1481826359268723
1109	20	1.122534380652617
1109	21	0.9791343720881969
1109	22	1.284992310361666
1109	23	1.646474790485982
1109	24	1.3811845764346202
1109	25	1.5749246205624488
1110	1	0.44556839360204137
1110	2	0.6341487206168601
1110	3	0.5617392470982949
1110	4	0.9212967836101384
1110	5	0.8272931865786362
1110	6	0.4556565017806875
1110	7	0.4285468156545704
1110	8	0.4611610072378909
1110	9	0.6636282685108412
1110	10	0.9793587631326446
1110	11	0.6079914859907944
1110	12	0.4305341083913419
1110	13	0.35478932550971365
1110	14	1.0828174504211958
1110	15	0.8392776367193591
1110	16	0.9926703384524558
1110	17	1.2501746148403683
1110	18	0.9424329595212839
1110	19	0.6664051083844811
1110	20	0.5981006786934273
1110	21	0.3061953273934108
1110	22	0.5651180527534336
1110	23	-0.7664435060193924
1110	24	0.0956941531155735
1110	25	0.6274804430322831
1111	1	0.0810303603310274
1111	2	0.41759716582856043
1111	3	0.42009434876551666
1111	4	0.7400359696968764
1111	5	0.6680563323065414
1111	6	0.37011707263682325
1111	7	0.42139083529443866
1111	8	0.40325682679335983
1111	9	0.5693655780267974
1111	10	0.8225994822578002
1111	11	0.48927556662149385
1111	12	0.37895848810937593
1111	13	0.09732063760284859
1111	14	1.1194767872689724
1111	15	0.7044670599586524
1111	16	0.7695666052184507
1111	17	1.1395985311770502
1111	18	1.3776686779296838
1111	19	0.8036521804822669
1111	20	0.4967654008683766
1111	21	0.3109589501736494
1111	22	0.6790567434556962
1111	23	1.0285793173674733
1111	24	0.694644429653593
1111	25	0.9585346492759186
1112	1	-0.08566641146907727
1112	2	0.12680805740530587
1112	3	-0.05464906467049602
1112	4	0.276760565292399
1112	5	-0.055833242405149974
1112	6	-0.20680606332879137
1112	7	-0.2340450388524573
1112	8	-0.0027967356118949738
1112	9	0.029953011905313076
1112	10	0.38640373066352396
1112	11	-0.044701445166836606
1112	12	-0.2152702810554601
1112	13	-0.2331022201248132
1112	14	0.3600252038711389
1112	15	0.24272317959457723
1112	16	0.09117411700358957
1112	17	0.6062504011279941
1112	18	0.5928677619831362
1112	19	0.11137453098533712
1112	20	0.036453902285153106
1112	21	0.025274580750405562
1112	22	0.23224573418749372
1112	23	0.6093984243195862
1112	24	0.33502513593025895
1112	25	0.6293563787006988
1113	1	0.13246978693769051
1113	2	0.15561558015659926
1113	3	0.08712927454938324
1113	4	0.4921956113105992
1113	5	0.4818637851026466
1113	6	0.1503044576952135
1113	7	0.19713068106813836
1113	8	0.16740693037588983
1113	9	0.26455431639885063
1113	10	0.5631418522959493
1113	11	0.17097503527864433
1113	12	0.18801815123204335
1113	13	0.033321508251788146
1113	14	0.7119852956497397
1113	15	0.38628192047472965
1113	16	0.5163362763786736
1113	17	0.8425086186638922
1113	18	0.7534671587193245
1113	19	0.502452413890282
1113	20	0.37689165525635815
1113	21	0.27825790076381196
1113	22	0.34392739651443804
1113	23	0.476742001106814
1113	24	0.23604952428778603
1113	25	0.7858760105550928
1114	1	-1.0270050956258743
1114	2	-0.7595648243831796
1114	3	-0.6670287349440791
1114	4	-0.48093875075496584
1114	5	-3.756224218313683
1114	6	-1.3083587378898365
1114	7	-1.017630175642985
1114	8	-0.6657604452893082
1114	9	-0.4444702220677428
1114	10	-0.24450824871581034
1114	11	-0.8515103467593264
1114	12	-0.8586915754613429
1114	13	-0.772774407752415
1114	14	-6.249338184246458
1114	15	-2.569572655440126
1114	16	-1.337398387079709
1114	17	-0.43911709580953584
1114	18	-6.25918754120184
1114	19	-2.4694398320199724
1114	20	-1.520047093019052
1114	21	-1.3067788083880174
1114	22	-1.0230275916597904
1114	23	-0.6180422402373793
1114	24	-0.6082486492881998
1114	25	-0.4991095767913407
1115	1	-0.8867139256866494
1115	2	-0.5264158158901147
1115	3	-0.6138718372504535
1115	4	-3.11543841960334
1115	5	-1.1333260571791968
1115	6	-0.7751715008631876
1115	7	-0.7785231162694473
1115	8	-0.5958402138727621
1115	9	-0.3279262762986903
1115	10	-0.7245887477807926
1115	11	-0.7985845247241059
1115	12	-0.8418427753990088
1115	13	-0.8969730695102456
1115	14	-6.4463522192316525
1115	15	-2.6168591600372935
1115	16	-1.4067510343447864
1115	17	-0.5703661218341551
1115	18	-0.3295360782908219
1115	19	-0.6425917137404014
1115	20	-0.7401326567559963
1115	21	-0.8298474152136421
1115	22	-2.08336974921005
1115	23	-1.1073764793678151
1115	24	-0.7993679417924824
1115	25	-0.3146459450319483
1116	1	-0.15838161986117072
1116	2	0.07864360015923211
1116	3	-0.03740356114767707
1116	4	0.24225451538313791
1116	5	-0.3484738062846162
1116	6	-0.16528981105312823
1116	7	-0.07516903706326872
1116	8	-0.03748951076381957
1116	9	-0.03386918600521227
1116	10	0.13955905677742209
1116	11	-0.1310412144526154
1116	12	-0.36035716213991265
1116	13	-0.35587569112313844
1116	14	0.6161673931206195
1116	15	0.013741165503290631
1116	16	0.022841681113824563
1116	17	0.5955595975028913
1116	18	0.38651277889211144
1116	19	0.21530835378154478
1116	20	-0.307090287551226
1116	21	-0.2500125014753678
1116	22	-0.41541233696410984
1116	23	-0.9067178957629238
1116	24	-0.43998695819020384
1116	25	0.03568611941073385
1201	1	0.8298717255870898
1201	2	1.1074321152167954
1201	3	1.0599706928302624
1201	4	1.4085766432586055
1201	5	1.2964395982953434
1201	6	1.0259087043758086
1201	7	0.9078617968640543
1201	8	0.989928082103475
1201	9	1.1636031142855217
1201	10	1.6314649024127448
1201	11	1.0813851695178016
1201	12	1.103474834946958
1201	13	1.0395828761194252
1201	14	1.937104137476119
1201	15	1.6174928668478188
1201	16	1.6714423223929913
1201	17	2.0886295873505887
1201	18	1.4677739106070717
1201	19	1.4443603071435689
1201	20	1.34260806398094
1201	21	1.3231591560348974
1201	22	1.6149739360901592
1201	23	1.8150377793413384
1201	24	1.4637325473004452
1201	25	1.7086759861349705
1202	1	0.7997035219914537
1202	2	1.017342356347374
1202	3	0.955597466455341
1202	4	1.3072939068320366
1202	5	1.1581583734849943
1202	6	0.8098565685704884
1202	7	0.813855856318817
1202	8	0.9299531293259804
1202	9	1.115354676417823
1202	10	1.3536251436527422
1202	11	0.9720701938377374
1202	12	0.8831409779971224
1202	13	0.9834769809912416
1202	14	1.8610542747120817
1202	15	1.4863615823737817
1202	16	1.5551391307833455
1202	17	1.8769827456013886
1202	18	2.0076428066049274
1202	19	1.5783969410408005
1202	20	1.2935310894368115
1202	21	1.1249772389098638
1202	22	1.329539841375535
1202	23	1.5927433148679668
1202	24	1.3421650329411108
1202	25	1.69377684249379
1203	1	0.775174288464374
1203	2	0.8417056420567839
1203	3	0.8328198947734293
1203	4	1.1140380043372815
1203	5	1.04412333106702
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2211	17	-1.8843508807666893
2211	18	-0.5890426082491373
2211	19	-0.4964346119967047
2211	20	-0.6448232351167249
2211	21	-0.5421728802109556
2211	22	-0.40125273255331706
2211	23	-0.04084966151721403
2211	24	-0.3866761710365907
2211	25	-7.3805043100766135
2212	1	-1.4718483629223762
2212	2	-3.844522074564786
2212	3	-1.687192582223176
2212	4	-3.644217886588482
2212	5	-1.1856247779005002
2212	6	-1.011247375323876
2212	7	-0.8683378414235676
2212	8	-0.7593371958939983
2212	9	-0.8509694317810812
2212	10	-0.32022478276998356
2212	11	-0.8180937938460389
2212	12	-0.8461744625621037
2212	13	-1.0542156805729377
2212	14	-6.435237342371643
2212	15	-5.086929525745308
2212	16	-2.7927021011276523
2212	17	-7.396494501593793
2212	18	-2.989289367941467
2212	19	-1.8290430871628232
2212	20	-1.8992032699275114
2212	21	-1.419162909656471
2212	22	-1.2227315085679393
2212	23	-0.7752298949020684
2212	24	-1.0632377329548106
2212	25	-0.8295717597786236
2213	1	-0.15565372229201557
2213	2	-0.13358128368596311
2213	3	-0.12320590289896316
2213	4	0.2792717843818693
2213	5	0.13138225696039285
2213	6	-0.23172513113851778
2213	7	-0.1789678205676637
2213	8	-0.2241170290104222
2213	9	0.08347657973050815
2213	10	-3.6866772634251745
2213	11	-1.345332809098629
2213	12	-0.7553055631575276
2213	13	-0.5815767334484505
2213	14	0.22308484300332765
2213	15	-0.30955313497321413
2213	16	-0.21472913575863117
2213	17	0.20572674045515527
2213	18	0.40392777250586676
2213	19	-0.22453955400120407
2213	20	-0.5003490440798615
2213	21	-0.49320271800234394
2213	22	-0.7287898264574864
2213	23	-0.8889965495283363
2213	24	-0.4620334509287076
2213	25	-0.09435195592218548
2214	1	-0.774397189063432
2214	2	-0.5479553626745108
2214	3	-0.5198589376594391
2214	4	-0.03470019971417315
2214	5	-3.3279614356751246
2214	6	-1.2649809876030034
2214	7	-0.9091484822702114
2214	8	-0.6663196003590457
2214	9	-0.48562062051298227
2214	10	-4.188520972329059
2214	11	-1.7492131401687843
2214	12	-1.1475163278477183
2214	13	-0.6861054329196392
2214	14	-0.03078007748576539
2214	15	-0.37146273372427885
2214	16	-0.3594314749132792
2214	17	-8.595230083940919E-4
2214	18	0.24403793597634404
2214	19	-0.46576696980943666
2214	20	-0.6995712977871236
2214	21	-0.6916323768339652
2214	22	-0.45855182911130754
2214	23	-0.2177069057345946
2214	24	-0.6721340367674173
2214	25	-0.2682534122073079
2215	1	-2.1517942889236323
2215	2	-4.666438809811911
2215	3	-2.8097284849884225
2215	4	-1.8055349224715158
2215	5	-1.728134812654833
2215	6	-1.9348842096534256
2215	7	-2.0852541220312055
2215	8	-2.2220986720211684
2215	9	-7.785107343904976
2215	10	-3.6412729110780617
2215	11	-2.99264323487019
2215	12	-3.177077293768157
2215	13	-2.653476792308183
2215	14	-7.829674696569764
2215	15	-4.567769562275014
2215	16	-9.286215013806167
2215	17	-4.471740502378363
2215	18	-2.9141131160130946
2215	19	-3.3856031090707077
2215	20	-3.176679364675447
2215	21	-3.2507496523570794
2215	22	-9.49189459269316
2215	23	-10.18080931829141
2215	24	-6.406549471356016
2215	25	-4.795703099537015
2216	1	-1.805589481110843
2216	2	-1.7763829748440614
2216	3	-1.5990649590217672
2216	4	-6.588348333329911
2216	5	-3.36702359771326
2216	6	-2.5306843630588745
2216	7	-1.9903720014801358
2216	8	-7.739878086167071
2216	9	-3.801463946620565
2216	10	-2.1363622627907937
2216	11	-1.9873507523059857
2216	12	-2.1084181439576426
2216	13	-1.9284985032583606
2216	14	-1.2355719816595254
2216	15	-1.8603927580694588
2216	16	-1.5908203355201707
2216	17	-1.3081998279133629
2216	18	-7.338979685288592
2216	19	-4.49934448962982
2216	20	-3.76890305515073
2216	21	-3.0189404112037472
2216	22	-2.7531014707048413
2216	23	-1.8910088304254629
2216	24	-2.3036624011796007
2216	25	-1.768956660736869
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	112675.0
3	5.0
4	936.0
5	5587.0
6	6820.0
7	5987.0
8	5706.0
9	5390.0
10	5024.0
11	4807.0
12	4833.0
13	4746.0
14	4697.0
15	4862.0
16	5015.0
17	5256.0
18	5938.0
19	7375.0
20	9384.0
21	11887.0
22	14816.0
23	17660.0
24	20611.0
25	23578.0
26	26788.0
27	29831.0
28	33581.0
29	40130.0
30	48244.0
31	57796.0
32	73977.0
33	95853.0
34	125491.0
35	166890.0
36	236304.0
37	342743.0
38	936283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23509034929093	18.629941482522668	12.364144440599926	25.770823727586478
2	14.35807068935597	20.24271926183787	41.51854082147567	23.88066922733049
3	18.223308766138217	25.052621140583213	29.755455644696944	26.96861444858163
4	11.327335692268365	16.936651335325244	39.18290988833457	32.55310308407182
5	12.646608526395633	38.032429907999436	36.32828634410025	12.992675221504676
6	31.579847981543352	37.28345187837247	18.41408914969861	12.722610990385567
7	27.73978927382636	31.568121034923585	22.781202571154488	17.91088712009557
8	24.92912084317877	36.58281892429611	20.981227593491084	17.506832639034034
9	26.576311019777783	14.84681634554633	20.209799860872675	38.367072773803216
10	13.966486463631442	28.617699254124574	35.817018596426706	21.59879568581728
11	34.297497255692846	22.18564918819565	24.89529351322373	18.62156004288778
12	23.147818309976582	24.614923722725134	31.910759397983355	20.32649856931493
13	28.888931439155048	21.819670110445863	25.72410912707198	23.567289323327106
14	20.41397742231441	21.58243248633771	28.680561649846425	29.32302844150146
15	23.159849669656253	30.406665921930358	23.361367335865438	23.072117072547957
16	22.232039183102987	27.635990281607608	27.81429111060675	22.31767942468266
17	21.612183277397694	28.179554206503855	28.224797442759726	21.983465073338728
18	21.622229713152766	27.1314279331481	29.75831110987001	21.488031243829127
19	23.527682395215695	26.863483619374644	27.39298980718539	22.215844178224266
20	23.802227462193816	27.13596104035465	27.544529896493497	21.517281600958036
21	23.33434796328662	26.612254309617455	26.792174929118858	23.26122279797707
22	23.401043156584002	27.670261020550736	26.916519944095807	22.01217587876945
23	22.35942237115047	27.415908728259136	27.635870432423808	22.588798468166587
24	23.25216181849303	26.990323336148865	27.57528011972842	22.182234725629694
25	22.90268683964018	27.009983292028846	27.576370747805385	22.51095912052559
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	253.0
1	253.0
2	372.0
3	491.0
4	491.0
5	491.0
6	945.0
7	1399.0
8	1399.0
9	1399.0
10	2348.0
11	3297.0
12	3297.0
13	3297.0
14	6540.5
15	9784.0
16	9784.0
17	9784.0
18	18870.0
19	27956.0
20	27956.0
21	27956.0
22	44830.5
23	61705.0
24	61705.0
25	61705.0
26	88686.5
27	115668.0
28	115668.0
29	115668.0
30	149303.5
31	182939.0
32	182939.0
33	182939.0
34	210750.0
35	238561.0
36	238561.0
37	238561.0
38	261428.5
39	284296.0
40	284296.0
41	284296.0
42	299753.5
43	315211.0
44	315211.0
45	315211.0
46	324367.5
47	333524.0
48	333524.0
49	333524.0
50	321045.0
51	308566.0
52	308566.0
53	308566.0
54	274490.5
55	240415.0
56	240415.0
57	240415.0
58	206162.5
59	171910.0
60	171910.0
61	171910.0
62	140021.0
63	108132.0
64	108132.0
65	108132.0
66	83692.0
67	59252.0
68	59252.0
69	59252.0
70	43100.0
71	26948.0
72	26948.0
73	26948.0
74	19008.5
75	11069.0
76	11069.0
77	11069.0
78	7592.0
79	4115.0
80	4115.0
81	4115.0
82	2760.0
83	1405.0
84	1405.0
85	1405.0
86	934.0
87	463.0
88	463.0
89	463.0
90	290.5
91	118.0
92	118.0
93	118.0
94	67.0
95	16.0
96	16.0
97	16.0
98	14.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18600154894943421
2	0.26289069697141304
3	0.2636085417143568
4	0.4684734552978138
5	0.26093656406006605
6	0.017627076465619625
7	0.018384801472060286
8	0.016829471195682084
9	0.01794611857359464
10	0.020697856754879153
11	0.019860371221444736
12	0.026520375225423186
13	0.02660013575241694
14	0.02416743967910745
15	0.04091715034779578
16	0.02021929359291663
17	0.04203379772570833
18	0.03074768315609215
19	0.025603129164995016
20	0.02819534629229202
21	0.033698822654861044
22	0.042871283259142745
23	0.028155466028795147
24	0.04654026750085543
25	0.03661008189013307
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2507506.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.56083435988337
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.22073856374013	60.38566226230706
2	11.295555683887915	16.392298899628603
3	2.9324084760086757	6.383340171095507
4	1.085491000644776	3.150565307877186
5	0.4588401504445007	1.664691207703369
6	0.26744652445825645	1.1643685776805226
7	0.16185115968201783	0.8220838632049352
8	0.11848278001426835	0.6877767500091061
9	0.08195275988758544	0.5351904571984543
>10	0.3444092809460195	4.292197499504262
>50	0.019132911157423906	0.9565883336334473
>100	0.01119212471969064	1.5824661886795968
>500	0.0016657229392139494	0.7844219383659294
>1k	8.328614695911415E-4	1.198348543112123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	3345	0.1333994813970535	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3170	0.12642043528510002	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2889	0.11521408124247759	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2660	0.10608150090169274	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.988026349687698E-5	0.0
2	0.0	0.0	0.0	3.988026349687698E-5	0.0
3	0.0	0.0	0.0	3.988026349687698E-5	0.0
4	0.0	0.0	0.0	3.988026349687698E-5	0.0
5	0.0	0.0	0.0	3.988026349687698E-5	0.0
6	0.0	0.0	0.0	3.988026349687698E-5	3.988026349687698E-5
7	0.0	0.0	0.0	3.988026349687698E-5	3.988026349687698E-5
8	0.0	0.0	0.0	3.988026349687698E-5	3.988026349687698E-5
9	0.0	0.0	0.0	1.1964079049063093E-4	7.976052699375396E-5
10	0.0	0.0	0.0	1.1964079049063093E-4	7.976052699375396E-5
11	0.0	0.0	0.0	1.1964079049063093E-4	7.976052699375396E-5
12	0.0	0.0	0.0	1.1964079049063093E-4	1.9940131748438488E-4
13	0.0	0.0	0.0	1.1964079049063093E-4	1.9940131748438488E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAACT	145	1.0004442E-10	11.780298	19
TAGGACC	710	0.0	11.139583	4
TAGGACT	450	0.0	11.011336	4
GCCGGTT	70	0.0015060374	10.844921	11
GTCCTAC	1865	0.0	10.654675	1
GGACGTG	1680	0.0	10.559341	6
GGCGAGG	840	0.0	10.393464	19
GACGTGG	905	0.0	10.377875	7
TGTAGGA	2015	0.0	10.364308	2
GTATTAG	305	0.0	10.3364105	1
AGGACGT	1815	0.0	10.32296	5
AAAGGCG	195	1.4551915E-11	10.242345	5
ACGTGGA	940	0.0	10.193313	8
TCCTACA	1975	0.0	10.091377	2
TAAGACT	340	0.0	10.089573	4
GTCCTAA	1090	0.0	10.079222	1
CTAAGGT	180	3.7289283E-10	10.058433	4
GTAGGAC	1950	0.0	10.018111	3
TTAGGAC	1285	0.0	9.937298	3
CCTACAG	2005	0.0	9.885885	3
>>END_MODULE