FastQCFastQC Report
Thu 26 May 2016
SRR1513185_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513185_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507506
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT34040.13575241694336923No Hit
GTCCTAAAGTGTGTATTTCTCATTT33420.13327984060656287No Hit
CTTTAGGACGTGAAATATGGCGAGG33280.13272151691760659No Hit
GTATCAACGCAGAGTACTTTTTTTT32790.1307673840062596No Hit
CTGTAGGACGTGGAATATGGCAAGA29750.11864378390320901No Hit
GTACATGGGAAGCAGTGGTATCAAC27670.11034868909585858No Hit
CCCATGTACTCTGCGTTGATACCAC25960.10352916403789264No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG604.0160667E-412.6954015
TAGGACC6150.012.0761154
GGCGAGG9650.011.79848319
GGACCGT700.001465480110.8804666
GAAGCGT803.698334E-410.710466
TGGCGAG20100.010.52894718
GGACGTG19400.010.3546716
AGGACGT20300.010.2720185
GTCCTAC17850.010.2447441
ACTAGAC1605.9517333E-910.1166493
TAGTACG951.6075486E-410.0226864
TAGGACG21200.010.0155944
TTAGGAC13750.09.9716623
TGTAGGA20200.09.94617752
GACCGTG1252.599456E-69.8715977
AGGACCT14050.09.8265125
GTAGGAC18900.09.8238233
CTAGGAC3200.09.81913
GACGTGG9150.09.7512637
CTAAGAC2750.09.6946713
Produced by FastQC (version 0.11.5)