FastQCFastQC Report
Thu 26 May 2016
SRR1513184_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513184_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215673
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT45970.14295607793454124No Hit
GTCCTACAGTGGACATTTCTAAATT44300.13776276381336036No Hit
CTTTAGGACGTGAAATATGGCGAGG41160.12799808935796642No Hit
CTGTAGGACGTGGAATATGGCAAGA37590.1168962142605918No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9250.012.9795634
TAGGCCG550.003046112812.1033535
GGCGAGG11700.011.76141319
AGGACGT25650.010.7147195
GTCCTAG5400.010.6119231
TTAGGAC17250.010.6059633
GTAGGAC25650.010.587553
GGACGTG25300.010.4246086
TGGCGAG26050.010.34604618
TGTAGGA28100.010.2145022
CTAATAC3300.010.1062983
TACACCG1701.5334081E-910.0691755
GTCCTAA14650.09.9744841
TAGGACG28000.09.9370174
GTGTAGG5000.09.932761
TCTATAC3650.09.920393
GTCCTAC22800.09.8858451
TTTAGGA19550.09.8528442
AGGACCT18750.09.8394495
GACGTGA14650.09.7793187
Produced by FastQC (version 0.11.5)