FastQCFastQC Report
Thu 26 May 2016
SRR1513184_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513184_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215673
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTAGGACGTGAAATATGGCGAGG48440.15063720720359314No Hit
GTCCTACAGTGGACATTTCTAAATT47310.14712316830722527No Hit
GTCCTAAAGTGTGTATTTCTCATTT46250.1438268132362961No Hit
CTGTAGGACGTGGAATATGGCAAGA41310.12846455469819226No Hit
GTACATGGGAAGCAGTGGTATCAAC33300.10355530553013319No Hit
CCCATGTACTCTGCGTTGATACCAC32800.10200042106271379No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG551.9260411E-413.8476624
TAGGACC10500.012.6936894
GACCGTC1157.171002E-811.5549157
TGGCGAG25350.011.15631818
GACCGAT600.00591708911.0734617
GGCGAGG12100.010.89977319
ACCGATC700.001505823310.845288
GGACCTG19150.010.7367566
ATACCGT909.3311595E-510.576596
TGTAGGA28650.010.4689432
AGGACCT20650.010.4192765
GTAGGAC26750.010.3571123
GGCGAGA14950.010.15469319
AGGACGT26800.010.1241655
ATAGGAC4800.010.1157563
TATGGCG27550.010.05937116
GTCCTAC26800.010.0579821
GGACGTG26600.09.912576
CTGTAGG28700.09.8235631
ATGGCGA28650.09.80580917
Produced by FastQC (version 0.11.5)