##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513184_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3215673 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.837857891645076 31.0 31.0 34.0 27.0 34.0 2 30.95359913772327 33.0 31.0 34.0 27.0 34.0 3 31.048097863184474 33.0 31.0 34.0 27.0 34.0 4 34.4449762149323 37.0 35.0 37.0 31.0 37.0 5 34.1892313055463 37.0 35.0 37.0 30.0 37.0 6 34.105638228762686 37.0 35.0 37.0 30.0 37.0 7 33.98179510167856 37.0 35.0 37.0 30.0 37.0 8 33.95166423949201 37.0 35.0 37.0 30.0 37.0 9 35.52686109564001 39.0 37.0 39.0 30.0 39.0 10 35.3650716350823 39.0 35.0 39.0 29.0 39.0 11 35.384123323484694 39.0 35.0 39.0 30.0 39.0 12 35.32238352593687 39.0 35.0 39.0 29.0 39.0 13 35.29853564090627 39.0 35.0 39.0 29.0 39.0 14 36.468570031840926 40.0 37.0 41.0 29.0 41.0 15 36.45097091650799 40.0 37.0 41.0 29.0 41.0 16 36.41152038780063 40.0 37.0 41.0 29.0 41.0 17 36.34587347656307 40.0 37.0 41.0 28.0 41.0 18 36.302515212212185 40.0 37.0 41.0 28.0 41.0 19 36.38187589347549 40.0 37.0 41.0 28.0 41.0 20 36.344454799975 40.0 37.0 41.0 28.0 41.0 21 36.29256115282866 40.0 37.0 41.0 28.0 41.0 22 36.23558521031212 40.0 37.0 41.0 28.0 41.0 23 36.15229409209208 40.0 37.0 41.0 28.0 41.0 24 36.09418308391432 40.0 37.0 41.0 27.0 41.0 25 36.03626643629499 40.0 37.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6156473965756106 1101 2 0.6635077854137847 1101 3 0.5706573347697699 1101 4 0.672915492966176 1101 5 0.6114444692855017 1101 6 0.5505826671926073 1101 7 0.6712223098952776 1101 8 0.6858836300414524 1101 9 0.7595872876410112 1101 10 0.8640742537158914 1101 11 0.8047179105565405 1101 12 0.7457173015341354 1101 13 0.7805210651124881 1101 14 0.8938128654878668 1101 15 0.9724597176764931 1101 16 0.9346703100380935 1101 17 0.8785132330556209 1101 18 0.923655837542924 1101 19 0.8723918482258455 1101 20 0.8831154205987346 1101 21 0.9081849499116785 1101 22 0.9808794951795434 1101 23 0.9662507413279116 1101 24 0.975652593946684 1101 25 1.02868832716009 1102 1 0.443636913433906 1102 2 0.5371913100054435 1102 3 0.40935261775632625 1102 4 0.5475585933215328 1102 5 0.6397551346634245 1102 6 0.5555149657931224 1102 7 0.505883487086976 1102 8 0.5696559775526211 1102 9 0.4900337685960565 1102 10 0.6607819031444748 1102 11 0.6429933874865554 1102 12 0.5801629266861994 1102 13 0.684725575336536 1102 14 0.7710405887005436 1102 15 0.6664507708879555 1102 16 0.6519569964748158 1102 17 0.7658494395265407 1102 18 0.7005628125596104 1102 19 0.8264906952492836 1102 20 0.7539986368522307 1102 21 0.7620473835858377 1102 22 0.7565282085171248 1102 23 0.762054726861102 1102 24 0.8336908035036501 1102 25 0.8272999158177683 1103 1 0.4224441223244355 1103 2 0.4354775146918719 1103 3 0.40606885731397213 1103 4 0.5089782102813274 1103 5 0.5148147199023612 1103 6 0.3191311434299209 1103 7 0.41132057224443486 1103 8 0.4902128306698472 1103 9 0.5897928204466254 1103 10 0.5444958505443438 1103 11 0.5428363040672224 1103 12 0.5282145925743293 1103 13 0.5593265117535964 1103 14 0.5484785863757935 1103 15 0.5485011114526301 1103 16 0.6030012861774878 1103 17 0.6427533970292885 1103 18 0.5320635784227576 1103 19 0.5951102948277835 1103 20 0.6232089830658225 1103 21 0.5669964889925083 1103 22 0.580724921280428 1103 23 0.5360318921897687 1103 24 0.5262218454034269 1103 25 0.5646866386839378 1104 1 0.2706205378742297 1104 2 0.3829528309555812 1104 3 0.3605040686477672 1104 4 0.5176544955232103 1104 5 0.4408625450419592 1104 6 0.3867241213288395 1104 7 0.37512146175614447 1104 8 0.46176964705531987 1104 9 0.45197473029063673 1104 10 0.6035285813281988 1104 11 0.568832176937974 1104 12 0.5764293741910933 1104 13 0.579338047208033 1104 14 0.6401632326859428 1104 15 0.6169125084816045 1104 16 0.6279752886811067 1104 17 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-0.5874660019155833 2216 2 -0.5769513966030182 2216 3 -0.6084430568886638 2216 4 -0.3775279639568083 2216 5 -0.67837171565521 2216 6 -0.7223238620457053 2216 7 -0.8970042644309189 2216 8 -0.8577362649081195 2216 9 -1.109923579991424 2216 10 -1.0685848488250116 2216 11 -1.028548084739846 2216 12 -0.9127619566496392 2216 13 -1.0628282407104308 2216 14 -1.3057244271055666 2216 15 -1.2115536447876991 2216 16 -1.1933965084677283 2216 17 -1.372030032599504 2216 18 -1.2456838631391207 2216 19 -1.3484428187253243 2216 20 -1.2615967266883388 2216 21 -1.563563535175767 2216 22 -1.3349716158594092 2216 23 -1.3791465376278325 2216 24 -1.4871281036166266 2216 25 -1.5752767312794305 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74006.0 3 1.0 4 1242.0 5 10331.0 6 11239.0 7 8638.0 8 7053.0 9 6191.0 10 5570.0 11 5133.0 12 4693.0 13 4573.0 14 4503.0 15 4526.0 16 4416.0 17 4850.0 18 5262.0 19 6462.0 20 8054.0 21 10685.0 22 13210.0 23 16219.0 24 18694.0 25 21054.0 26 23434.0 27 25620.0 28 29516.0 29 35414.0 30 44745.0 31 57605.0 32 73583.0 33 96602.0 34 128800.0 35 172550.0 36 248618.0 37 404509.0 38 1618072.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.9623920067047 19.550486184024003 13.893678205683416 27.593443603587886 2 13.786292484552485 20.422348171489492 39.7174351963237 26.073924147634326 3 16.377260192773168 24.74264412038107 29.945989552444434 28.934106134401333 4 11.32473821566203 17.512308653001718 37.39967732545845 33.763275805877804 5 13.083131346706157 37.99141261168398 35.794800260435146 13.130655781174722 6 32.31303766141543 37.10536135714384 17.927205652020355 12.654395329420383 7 28.166255229032235 31.343895501476943 22.592343278345027 17.897505991145795 8 25.42813795214111 36.48993127025923 20.47945976644702 17.602471011152645 9 26.403284829866745 14.684362569036505 19.847588671031556 39.0647639300652 10 14.708223917043068 29.10228676418452 34.58029548929141 21.60919382948101 11 34.27229696551857 21.903812980984075 24.733422832483278 19.090467221014077 12 23.07725319085616 24.610929034139975 31.668705120203455 20.64311265480041 13 28.73703265226284 21.653663167865638 25.791086344911314 23.81821783496021 14 21.038419687419243 21.60767280431718 27.561304720058388 29.792602788205187 15 23.288655283046502 30.678461398282725 22.8632699904499 23.169613328220873 16 22.67590641212586 27.57966994778387 27.233521567646957 22.51090207244331 17 22.085057781587654 28.399508808294023 27.35028326259285 22.165150147525473 18 22.047921581509243 27.32055225410856 28.931531887996336 21.699994276385862 19 23.80472143778301 26.805119799183565 26.911660482891143 22.47849828014229 20 23.941022610196995 27.025011560566014 27.273140023876806 21.760825805360184 21 23.37695455052918 26.39906183440482 26.68236495599472 23.541618659071283 22 23.694524339740404 27.502132528026067 26.462441540122157 22.340901592111372 23 22.594426187396614 27.310518640796566 27.059111472831137 23.03594369897568 24 23.515481829153646 26.690960181585627 27.36397637446345 22.429581614797275 25 23.127222518514834 26.946619128547283 27.02769100637038 22.898467346567504 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 76.0 1 76.0 2 172.0 3 268.0 4 268.0 5 268.0 6 663.5 7 1059.0 8 1059.0 9 1059.0 10 1733.5 11 2408.0 12 2408.0 13 2408.0 14 5386.5 15 8365.0 16 8365.0 17 8365.0 18 17721.5 19 27078.0 20 27078.0 21 27078.0 22 47107.5 23 67137.0 24 67137.0 25 67137.0 26 101699.5 27 136262.0 28 136262.0 29 136262.0 30 180474.5 31 224687.0 32 224687.0 33 224687.0 34 261660.0 35 298633.0 36 298633.0 37 298633.0 38 331054.5 39 363476.0 40 363476.0 41 363476.0 42 386411.5 43 409347.0 44 409347.0 45 409347.0 46 425132.0 47 440917.0 48 440917.0 49 440917.0 50 426034.5 51 411152.0 52 411152.0 53 411152.0 54 365934.5 55 320717.0 56 320717.0 57 320717.0 58 274696.0 59 228675.0 60 228675.0 61 228675.0 62 185813.0 63 142951.0 64 142951.0 65 142951.0 66 109884.0 67 76817.0 68 76817.0 69 76817.0 70 55815.5 71 34814.0 72 34814.0 73 34814.0 74 24206.0 75 13598.0 76 13598.0 77 13598.0 78 9309.0 79 5020.0 80 5020.0 81 5020.0 82 3341.5 83 1663.0 84 1663.0 85 1663.0 86 1057.0 87 451.0 88 451.0 89 451.0 90 271.0 91 91.0 92 91.0 93 91.0 94 50.5 95 10.0 96 10.0 97 10.0 98 5.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18605747537140746 2 0.008365278434716466 3 0.005721974840103456 4 0.001523786778071029 5 0.08051191772297743 6 0.3292934325100842 7 0.028889753404652774 8 0.014647011683090912 9 0.005100021053135689 10 0.0038872111685485434 11 0.0 12 0.0 13 0.0 14 0.0017414706035097476 15 0.0 16 0.0 17 0.0197470327362266 18 0.02864097188986567 19 0.0 20 0.0 21 0.0011195168165419806 22 1.8658613609033006E-4 23 0.005348802567922795 24 0.0 25 2.4878151478710677E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3215673.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.8236112929403 #Duplication Level Percentage of deduplicated Percentage of total 1 77.92928492371416 50.5165767423164 2 13.708457161829646 17.77263396968737 3 4.155254330256125 8.0807577458349 4 1.6795282013750297 4.354923331258643 5 0.8268780650893225 2.680061113900441 6 0.4709387223706521 1.8316769209049448 7 0.2745611575266422 1.2458632026152763 8 0.18541185217781103 0.9615252667742822 9 0.14493173622312402 0.8455498679644872 >10 0.583063339455539 6.317910066522245 >50 0.024785613436979454 1.0935549704273904 >100 0.013882148590419436 1.7672895330627203 >500 0.001823244798707534 0.8985186939535156 >1k 0.0011995031558600273 1.6331585747773898 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTTAGGACGTGAAATATGGCGAGG 4844 0.15063720720359314 No Hit GTCCTACAGTGGACATTTCTAAATT 4731 0.14712316830722527 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4625 0.1438268132362961 No Hit CTGTAGGACGTGGAATATGGCAAGA 4131 0.12846455469819226 No Hit GTACATGGGAAGCAGTGGTATCAAC 3330 0.10355530553013319 No Hit CCCATGTACTCTGCGTTGATACCAC 3280 0.10200042106271379 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 3.1097689348388346E-5 0.0 0.0 0.0 0.0 11 3.1097689348388346E-5 0.0 0.0 0.0 0.0 12 9.329306804516505E-5 0.0 0.0 0.0 3.1097689348388346E-5 13 9.329306804516505E-5 0.0 0.0 0.0 3.1097689348388346E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACG 55 1.9260411E-4 13.847662 4 TAGGACC 1050 0.0 12.693689 4 GACCGTC 115 7.171002E-8 11.554915 7 TGGCGAG 2535 0.0 11.156318 18 GACCGAT 60 0.005917089 11.073461 7 GGCGAGG 1210 0.0 10.899773 19 ACCGATC 70 0.0015058233 10.84528 8 GGACCTG 1915 0.0 10.736756 6 ATACCGT 90 9.3311595E-5 10.57659 6 TGTAGGA 2865 0.0 10.468943 2 AGGACCT 2065 0.0 10.419276 5 GTAGGAC 2675 0.0 10.357112 3 GGCGAGA 1495 0.0 10.154693 19 AGGACGT 2680 0.0 10.124165 5 ATAGGAC 480 0.0 10.115756 3 TATGGCG 2755 0.0 10.059371 16 GTCCTAC 2680 0.0 10.057982 1 GGACGTG 2660 0.0 9.91257 6 CTGTAGG 2870 0.0 9.823563 1 ATGGCGA 2865 0.0 9.805809 17 >>END_MODULE