FastQCFastQC Report
Thu 26 May 2016
SRR1513183_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513183_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160099
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6700.012.9434754
TAGGACT6300.011.7988144
TTAGGAC11150.011.0258723
GGACGTG13350.010.9439496
GACGTGA8500.010.82665357
AGGACGT13950.010.7042055
GGCGAGG6950.010.64927419
GTAGGAC16100.010.5955933
CCGCGCT909.628352E-510.54391917
TGGCGAG15300.010.41904818
CGCAGAT2250.010.1197598
TGTAGGA18200.010.1128642
CTAGGAC3800.010.0316993
TAGGACG15600.09.9574314
TCGAACT2107.2759576E-129.94062219
GTCTTAG5400.09.9060771
GTATAAT3800.09.8036651
TATGGCG15800.09.78982816
GTATCAA34650.09.7590481
GGCGAGA8950.09.54178619
Produced by FastQC (version 0.11.5)