FastQCFastQC Report
Thu 26 May 2016
SRR1513183_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513183_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160099
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7450.011.7554034
GCACCGT1156.893424E-811.5878186
TGGCGAG16850.010.47736818
GGACGTG16450.010.4733146
AGGACGT16500.010.4424085
GACGTGA10100.010.3367267
GGCGAGG8300.010.17402919
GGACCGG856.4796E-410.0784816
GTCCTAA9500.010.0252721
GTAGGAC17700.010.0036733
ACACTCG1054.0206665E-59.971836
GACGTGG7700.09.7375137
CCTATAC2950.09.6809733
TAGGACG17800.09.6262794
TATGGCG18800.09.59302416
TGTAGGA18900.09.5709042
GTTCTAA3100.09.5240081
CCCTATA2002.5465852E-109.5205312
AGCACCG1901.0149961E-99.51932055
GCGTCAG800.00454894979.49204513
Produced by FastQC (version 0.11.5)