FastQCFastQC Report
Thu 26 May 2016
SRR1513182_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513182_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026664
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGGG551.9790477E-413.80336317
AATACCG951.025739E-612.0145315
GCGTAAT600.005654214411.141231
CCGATAC803.6736476E-410.7183743
TAGGACG10500.010.3440814
GGACGTG9500.010.2870846
CCATGCG1303.903897E-710.219469
ATTATAC2650.010.0667333
GTAGGAC11550.010.063623
TAGGACC5400.010.0567464
GTCCTAC10400.010.0087421
GTCCTAA7950.09.970051
TGGCGAG9350.09.94637618
AGGACGT10150.09.9331965
TAGAACA6550.09.7456294
TTAGGAC8500.09.639533
TAGACTG4150.09.62615
GTATAGG4300.09.5496251
ATACGTG900.00110188949.5115045
GACGTGA6000.09.4882437
Produced by FastQC (version 0.11.5)