##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513182_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3026664 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.69178739364528 31.0 30.0 34.0 16.0 34.0 2 28.584784435933425 31.0 29.0 34.0 16.0 34.0 3 28.74988502192513 31.0 30.0 34.0 16.0 34.0 4 31.826260860141726 35.0 32.0 37.0 17.0 37.0 5 31.927668879003416 35.0 32.0 37.0 17.0 37.0 6 32.27948427707866 36.0 33.0 37.0 22.0 37.0 7 32.21623113764858 36.0 33.0 37.0 19.0 37.0 8 32.09468807902034 36.0 33.0 37.0 18.0 37.0 9 33.24498722025306 38.0 33.0 39.0 17.0 39.0 10 32.81783574258656 38.0 32.0 39.0 13.0 39.0 11 33.24404294629335 38.0 33.0 39.0 17.0 39.0 12 33.22330724520462 38.0 33.0 39.0 17.0 39.0 13 33.26439274395837 38.0 33.0 39.0 17.0 39.0 14 33.43286635054304 39.0 32.0 41.0 10.0 41.0 15 33.81740027964782 39.0 33.0 41.0 11.0 41.0 16 33.64142071931341 39.0 33.0 41.0 10.0 41.0 17 33.252139649462244 38.0 32.0 41.0 10.0 41.0 18 32.9619148342862 38.0 32.0 41.0 10.0 41.0 19 33.588657346834665 39.0 33.0 41.0 10.0 41.0 20 33.78388317963276 39.0 33.0 41.0 10.0 41.0 21 33.82843156690006 39.0 34.0 41.0 2.0 41.0 22 33.504758043839686 39.0 33.0 41.0 2.0 41.0 23 33.019824466805694 39.0 32.0 41.0 2.0 41.0 24 33.22969711867587 39.0 33.0 41.0 2.0 41.0 25 32.84747596693918 39.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2550283731340208 1101 2 1.5641096973060762 1101 3 1.5325513540336715 1101 4 1.868187723515291 1101 5 1.799632306383085 1101 6 1.4922446851366118 1101 7 1.4462361035978333 1101 8 1.430210071786341 1101 9 1.963788843523396 1101 10 2.3003911790527454 1101 11 1.924629328532859 1101 12 1.8633990516077716 1101 13 1.7031637311827126 1101 14 2.6974916946499974 1101 15 2.279949194202395 1101 16 2.435130580079303 1101 17 2.8353093684800115 1101 18 2.8541579793161205 1101 19 2.4180046838263607 1101 20 1.9996985211387894 1101 21 2.0023681774598643 1101 22 2.2843471561582405 1101 23 2.635309120283992 1101 24 2.2949505517726863 1101 25 2.565333552933204 1102 1 0.9576661263802357 1102 2 1.1806366664945749 1102 3 1.0778605642008152 1102 4 1.3832155425780144 1102 5 1.3865603585093247 1102 6 1.133567553429458 1102 7 1.0366484098179143 1102 8 1.171252423796929 1102 9 1.5749452409587619 1102 10 1.7575782659259644 1102 11 1.3745144223807912 1102 12 1.2535835469965377 1102 13 1.2335211406679818 1102 14 2.2275855768805215 1102 15 1.8536219052856566 1102 16 1.8774598321307892 1102 17 2.2255185299070632 1102 18 2.531529299413357 1102 19 1.7848816993353296 1102 20 1.6369569431343933 1102 21 1.5604561338273797 1102 22 1.6975780472844164 1102 23 1.9972170801601976 1102 24 1.8165521284446484 1102 25 2.165843336517611 1103 1 0.7525424546361208 1103 2 1.0823231102203614 1103 3 0.8663873281212098 1103 4 1.2972603370001892 1103 5 1.2086207985725927 1103 6 0.902806377608762 1103 7 0.9136817671601776 1103 8 0.9481286534706115 1103 9 1.2603006773013234 1103 10 1.4713803777476713 1103 11 1.2280343803377747 1103 12 0.9968437614535475 1103 13 1.0278199719633605 1103 14 1.9623353763340887 1103 15 1.5630555056101088 1103 16 1.6077177753119614 1103 17 2.0211289819233045 1103 18 2.167060983034901 1103 19 1.5913518279223737 1103 20 1.32898123592296 1103 21 1.3623756125793847 1103 22 1.5577652701419282 1103 23 1.874567950826858 1103 24 1.6028235787914937 1103 25 1.860196153972069 1104 1 0.8634069921509386 1104 2 1.0824081836896688 1104 3 0.9527257422931683 1104 4 1.3580845204029686 1104 5 1.1551325980535871 1104 6 0.9171603638516856 1104 7 0.8237542288363215 1104 8 0.9279644282033104 1104 9 1.2728543136830908 1104 10 1.4791588821365593 1104 11 1.06356756017626 1104 12 0.9712864360908995 1104 13 1.086668777301739 1104 14 1.8914525801002426 1104 15 1.505756989919007 1104 16 1.646150900522116 1104 17 1.9909846624531795 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1107 19 1.4719133101698674 1107 20 1.2030572260346233 1107 21 1.135307560358072 1107 22 1.3289804309465296 1107 23 1.5367266837242397 1107 24 1.385888521354019 1107 25 1.6987907046879158 1108 1 0.6163052256486026 1108 2 0.8493370830996376 1108 3 0.6559970134990145 1108 4 1.089415716386256 1108 5 0.9745157168008767 1108 6 0.6097854622598788 1108 7 0.5775010025565877 1108 8 0.6123151814614758 1108 9 0.9220828092932152 1108 10 1.1471681895266599 1108 11 0.7715360995092411 1108 12 0.5379008377633809 1108 13 0.5580151920888028 1108 14 1.4767206146437104 1108 15 1.0786604385689316 1108 16 1.1311030983638446 1108 17 1.482140667446835 1108 18 1.7219389050724772 1108 19 1.1354096938656042 1108 20 0.9288288369061561 1108 21 0.9090524940002922 1108 22 1.1387174944663414 1108 23 1.4537335068217345 1108 24 1.052703374980652 1108 25 1.496699784298471 1109 1 0.5324807080331659 1109 2 0.7001157064595525 1109 3 0.6476530046452282 1109 4 0.8640065432729003 1109 5 0.9699676692349506 1109 6 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-3.479589498947721 2215 19 -3.9150080422137883 2215 20 -3.733937387921639 2215 21 -3.8069702466927318 2215 22 -9.796124977956762 2215 23 -11.018740253001976 2215 24 -7.446609403421746 2215 25 -5.894972661126332 2216 1 -2.4723799840328375 2216 2 -2.4731857407797477 2216 3 -2.329386615955219 2216 4 -7.051040502587636 2216 5 -3.988940263136488 2216 6 -3.179107339405302 2216 7 -2.786005878384131 2216 8 -8.645147217416284 2216 9 -4.899344289521199 2216 10 -3.0548050098624273 2216 11 -2.910371929193513 2216 12 -3.173651152197568 2216 13 -2.8753093999357873 2216 14 -2.2609217891307978 2216 15 -2.84577230890336 2216 16 -2.4635315588386852 2216 17 -2.2497611859442586 2216 18 -8.131078767920236 2216 19 -5.740922137171101 2216 20 -5.346280983337895 2216 21 -4.180787293520645 2216 22 -3.894625418052332 2216 23 -3.015858557649004 2216 24 -3.4943994129006555 2216 25 -2.7721579135598446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 181538.0 3 3.0 4 1513.0 5 8604.0 6 10152.0 7 9012.0 8 8455.0 9 7906.0 10 7463.0 11 7116.0 12 7006.0 13 6718.0 14 6956.0 15 6968.0 16 7159.0 17 7536.0 18 8511.0 19 10380.0 20 13281.0 21 16643.0 22 20636.0 23 24481.0 24 28769.0 25 32714.0 26 36907.0 27 41082.0 28 45466.0 29 54133.0 30 63999.0 31 76283.0 32 97113.0 33 123750.0 34 158556.0 35 207184.0 36 286703.0 37 403778.0 38 992190.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.972744787376044 18.105877240335253 12.479119550852314 25.442258421436392 2 14.937480232294345 19.444599918196886 40.767690751114856 24.85022909839391 3 18.719328811044278 23.942572670182038 29.272635734916136 28.065462783857548 4 11.759573269492735 16.157063261775864 38.26152703858503 33.82183643014637 5 13.244083352239889 37.54424547940124 35.74508112154238 13.466590046816496 6 33.02063585272464 36.34714971972555 17.6056568415021 13.02655758604771 7 28.84529593281972 31.112485071672495 21.702070568112497 18.340148427395288 8 25.87369906334213 35.964258629264236 20.119292112015625 18.04275019537801 9 26.53506192063003 14.907743614620909 19.54785845429082 39.009336010458235 10 14.269285486501357 28.537216069159584 34.8786947979954 22.31480364634366 11 35.28258465841535 22.161678621540187 23.527296832691032 19.028439887353436 12 23.101549244479756 24.30867633751969 31.16295455642062 21.42681986157994 13 29.0344371066657 21.10372045541597 25.46273116729508 24.39911127062325 14 21.186417432840475 20.56489710561946 28.059360858859332 30.189324602680728 15 23.908092023000794 29.069388103168407 23.08851444457555 23.934005429255244 16 23.28449564089227 27.337365395075736 26.34446303102564 23.033675933006354 17 22.602500657754696 27.37512246136435 27.324088629635852 22.698288251245106 18 22.55203362091075 27.048823823360664 28.105822847730273 22.293319707998318 19 23.846251465691257 26.757904157341546 26.848786929801488 22.547057447165713 20 24.052474858138098 26.626148858329778 26.929966323289804 22.391409960242314 21 23.96221644569523 26.26370157866644 26.513796186280324 23.260285789358008 22 23.848331820582708 26.56414338099434 26.570886304395398 23.016638494027557 23 23.37307126918155 26.358803420159273 26.8171909536047 23.450934357054475 24 23.4990554484017 26.468996725885013 26.799912073237042 23.232035752476246 25 23.629987688290655 26.235938623236905 26.776067359097034 23.358006329375407 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 114.0 1 114.0 2 249.5 3 385.0 4 385.0 5 385.0 6 768.5 7 1152.0 8 1152.0 9 1152.0 10 2113.0 11 3074.0 12 3074.0 13 3074.0 14 6396.0 15 9718.0 16 9718.0 17 9718.0 18 18689.0 19 27660.0 20 27660.0 21 27660.0 22 44910.0 23 62160.0 24 62160.0 25 62160.0 26 88986.0 27 115812.0 28 115812.0 29 115812.0 30 150535.0 31 185258.0 32 185258.0 33 185258.0 34 220360.0 35 255462.0 36 255462.0 37 255462.0 38 288334.0 39 321206.0 40 321206.0 41 321206.0 42 346676.0 43 372146.0 44 372146.0 45 372146.0 46 388897.0 47 405648.0 48 405648.0 49 405648.0 50 399949.0 51 394250.0 52 394250.0 53 394250.0 54 360307.0 55 326364.0 56 326364.0 57 326364.0 58 283244.0 59 240124.0 60 240124.0 61 240124.0 62 197176.0 63 154228.0 64 154228.0 65 154228.0 66 119805.0 67 85382.0 68 85382.0 69 85382.0 70 63095.5 71 40809.0 72 40809.0 73 40809.0 74 28662.5 75 16516.0 76 16516.0 77 16516.0 78 11365.5 79 6215.0 80 6215.0 81 6215.0 82 4158.0 83 2101.0 84 2101.0 85 2101.0 86 1387.5 87 674.0 88 674.0 89 674.0 90 416.0 91 158.0 92 158.0 93 158.0 94 96.5 95 35.0 96 35.0 97 35.0 98 24.0 99 13.0 100 13.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17501116741072018 2 0.2385134260030185 3 0.24925132092627394 4 0.4126655618198783 5 0.24545175810727587 6 0.017279750907269523 7 0.01777534605757362 8 0.01582600513304417 9 0.01757710799745198 10 0.020451559869215742 11 0.018568298298060174 12 0.02649782070292573 13 0.025473590725630595 14 0.023028654650797047 15 0.03974673105438859 16 0.019361250538546728 17 0.04030840555806657 18 0.030660819965480146 19 0.026035265229308574 20 0.027555090356907803 21 0.03333703377712227 22 0.04196038939241356 23 0.027753328417029446 24 0.0468833012187676 25 0.035980207912077455 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3026664.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.85200317247242 #Duplication Level Percentage of deduplicated Percentage of total 1 80.74172685087296 56.39971360138081 2 12.283894157027927 17.161092272540603 3 3.584421231618894 7.511370097275616 4 1.3649400073102658 3.813751748834849 5 0.6724654865734014 2.348653065075172 6 0.3638601255474854 1.52498151864475 7 0.23260395015254814 1.137349630478979 8 0.15212727042789967 0.8501115661239375 9 0.11315163668659588 0.7113481636322289 >10 0.46001351245134464 5.437844784931823 >50 0.020610259730687388 0.9644679491660966 >100 0.008371162965477628 1.0259074878095364 >500 0.0013846344851531874 0.6399828756796183 >1k 4.297141493481934E-4 0.47342523842606204 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 3.303967668693981E-5 0.0 2 0.0 0.0 0.0 3.303967668693981E-5 0.0 3 0.0 0.0 0.0 3.303967668693981E-5 0.0 4 0.0 0.0 0.0 3.303967668693981E-5 0.0 5 0.0 0.0 0.0 3.303967668693981E-5 0.0 6 0.0 0.0 0.0 3.303967668693981E-5 0.0 7 0.0 0.0 0.0 3.303967668693981E-5 0.0 8 0.0 0.0 0.0 3.303967668693981E-5 0.0 9 0.0 0.0 0.0 3.303967668693981E-5 0.0 10 0.0 0.0 0.0 3.303967668693981E-5 0.0 11 0.0 0.0 0.0 3.303967668693981E-5 0.0 12 0.0 0.0 0.0 3.303967668693981E-5 0.0 13 0.0 0.0 0.0 3.303967668693981E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGCGGG 55 1.9790477E-4 13.803363 17 AATACCG 95 1.025739E-6 12.014531 5 GCGTAAT 60 0.0056542144 11.14123 1 CCGATAC 80 3.6736476E-4 10.718374 3 TAGGACG 1050 0.0 10.344081 4 GGACGTG 950 0.0 10.287084 6 CCATGCG 130 3.903897E-7 10.21946 9 ATTATAC 265 0.0 10.066733 3 GTAGGAC 1155 0.0 10.06362 3 TAGGACC 540 0.0 10.056746 4 GTCCTAC 1040 0.0 10.008742 1 GTCCTAA 795 0.0 9.97005 1 TGGCGAG 935 0.0 9.946376 18 AGGACGT 1015 0.0 9.933196 5 TAGAACA 655 0.0 9.745629 4 TTAGGAC 850 0.0 9.63953 3 TAGACTG 415 0.0 9.6261 5 GTATAGG 430 0.0 9.549625 1 ATACGTG 90 0.0011018894 9.511504 5 GACGTGA 600 0.0 9.488243 7 >>END_MODULE