FastQCFastQC Report
Thu 26 May 2016
SRR1513182_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513182_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3026664
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGG456.8290555E-414.75940410
ATGTCGA1251.4351826E-912.1476917
TACACCG1501.6916601E-1011.4256535
TGTCGAG1201.2965211E-711.07101518
TAGGACC5250.010.8815754
TATGTCG1053.512192E-610.84597316
ACCGAGC1408.511051E-910.8448978
GTAGGAC9800.010.4934693
TGCGTTG6350.010.31221412
GTATTAG2250.010.1608531
GTCCTAC10500.010.1608531
TCTACGC750.002598800210.1566413
TAGACAG5000.010.092665
CTAGACG951.6080965E-410.0225034
TTAGACA3550.09.9236894
GGTCTAC4250.09.8620041
TGTACCG1752.4792826E-99.7934175
TGGCGAG9750.09.7327618
TTAGGAC7450.09.7135673
CTTAGAC2950.09.6832383
Produced by FastQC (version 0.11.5)