FastQCFastQC Report
Thu 26 May 2016
SRR1513181_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513181_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204500
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT53300.1663286004056795No Hit
GTCCTAAAGTGTGTATTTCTCATTT41680.13006709315025747No Hit
CTGTAGGACGTGGAATATGGCAAGA40330.12585426743641756No Hit
CTTTAGGACGTGAAATATGGCGAGG34490.10762989545951007No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCTCG350.002187992416.2665759
TAGGACC11750.013.7066274
TCCAACG3000.012.65158418
GGCGAGG10400.011.86104619
GTAGGAC28100.011.6325883
AGGACCT20550.011.569795
TGTAGGA30400.011.2935592
AGGACGT23850.011.1253365
GGACGTG23750.011.0635246
GGACCTG20150.010.8746246
GCACCGT1158.996449E-710.7231626
CTGTAGG30450.010.6972341
GACGTGG11900.010.5231317
GTCCTAA13250.010.4533771
TTAGGAC17150.010.4467753
GACGTGA12900.010.4427937
GTCCTAC29250.010.3849811
TGGCGAG25450.010.29028618
TAGGACG25800.010.1946214
TAGAACG750.002581040810.16507154
Produced by FastQC (version 0.11.5)