##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1513181_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3204500 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.424508971758463 31.0 30.0 34.0 25.0 34.0 2 29.317171789670777 31.0 30.0 34.0 22.0 34.0 3 29.483565298798563 31.0 30.0 34.0 23.0 34.0 4 32.57249617725074 37.0 33.0 37.0 22.0 37.0 5 32.714972694648154 37.0 33.0 37.0 25.0 37.0 6 33.06895865189577 37.0 35.0 37.0 26.0 37.0 7 33.02329661413637 37.0 35.0 37.0 26.0 37.0 8 32.90408768918708 37.0 35.0 37.0 26.0 37.0 9 34.155290684974254 39.0 35.0 39.0 25.0 39.0 10 33.74114838508348 39.0 34.0 39.0 19.0 39.0 11 34.18464877515993 39.0 35.0 39.0 25.0 39.0 12 34.17327445779373 39.0 35.0 39.0 25.0 39.0 13 34.2165857388048 39.0 35.0 39.0 25.0 39.0 14 34.45604306444063 39.0 34.0 41.0 17.0 41.0 15 34.88733905445467 40.0 34.0 41.0 22.0 41.0 16 34.732231861444845 40.0 34.0 41.0 18.0 41.0 17 34.35482352941177 39.0 34.0 41.0 17.0 41.0 18 34.094845997815575 39.0 33.0 41.0 17.0 41.0 19 34.72265657668903 40.0 34.0 41.0 18.0 41.0 20 34.927453580901854 40.0 35.0 41.0 22.0 41.0 21 34.96885598377282 40.0 35.0 41.0 22.0 41.0 22 34.65668341394913 40.0 35.0 41.0 18.0 41.0 23 34.208999843969416 39.0 34.0 41.0 10.0 41.0 24 34.456135746606336 40.0 34.0 41.0 16.0 41.0 25 34.10062505851147 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.955337146861563 1101 2 1.22679066056671 1101 3 1.185468615912015 1101 4 1.4613788182994227 1101 5 1.31872038680617 1101 6 1.097818504042145 1101 7 1.0445982305875319 1101 8 1.1420942810915946 1101 9 1.4300469308584027 1101 10 1.741534998655304 1101 11 1.3838787220550657 1101 12 1.2255222226892926 1101 13 1.1717246360259281 1101 14 2.16133271904566 1101 15 1.8225714457432147 1101 16 1.9242391703030322 1101 17 2.1982543850487914 1101 18 2.284072488769695 1101 19 1.8097853491417908 1101 20 1.4694009798075314 1101 21 1.4389805206096256 1101 22 1.7309609502465193 1101 23 1.914510106706551 1101 24 1.6041869462616631 1101 25 1.9258552643701279 1102 1 1.0273495856179373 1102 2 1.23296044186419 1102 3 1.1461731433354103 1102 4 1.4046741183651008 1102 5 1.3441647596705621 1102 6 1.171467730034152 1102 7 1.106452561285579 1102 8 1.1569581636835409 1102 9 1.4425398664462392 1102 10 1.7073761286737295 1102 11 1.338123524040384 1102 12 1.295078177775359 1102 13 1.2042595672416851 1102 14 2.200305003189122 1102 15 1.8076544621326534 1102 16 1.936945588158629 1102 17 2.217195365647605 1102 18 2.472055216113482 1102 19 1.835860914317223 1102 20 1.6512740351287292 1102 21 1.5571873414399633 1102 22 1.7365866033396244 1102 23 2.102930041421949 1102 24 1.8972435612918588 1102 25 2.1411107108185163 1103 1 0.6528217124006197 1103 2 0.903156201425638 1103 3 0.7001947574018281 1103 4 1.0948970350151583 1103 5 0.9804678349052622 1103 6 0.7308444987657623 1103 7 0.715922998057664 1103 8 0.7651047588170528 1103 9 1.1030074360462407 1103 10 1.4018447983749738 1103 11 1.0164725218587805 1103 12 0.8996802679677174 1103 13 0.9605193824565674 1103 14 1.75397400026349 1103 15 1.3691089341504892 1103 16 1.536024585525297 1103 17 1.8657239138062636 1103 18 2.043731595026614 1103 19 1.4506974004769404 1103 20 1.0868094310190273 1103 21 1.2252331205321951 1103 22 1.5220307227139003 1103 23 1.7569285374188013 1103 24 1.509378687737069 1103 25 1.6952282515873662 1104 1 0.7997188066024385 1104 2 1.0213752202169601 1104 3 0.9045837395388752 1104 4 1.3132018281105786 1104 5 1.2289028542405802 1104 6 1.018019861928181 1104 7 1.0050727192452271 1104 8 1.0077151594305747 1104 9 1.2993107394465255 1104 10 1.4418105831704722 1104 11 1.1045991212659985 1104 12 1.0369477722137646 1104 13 1.0452323378421156 1104 14 2.048461183083006 1104 15 1.5578166039275558 1104 16 1.6920604369264964 1104 17 2.1249156399859075 1104 18 2.2934292058171124 1104 19 1.6521199884454916 1104 20 1.3217254801080784 1104 21 1.2780181861940392 1104 22 1.5437052093611214 1104 23 1.9301806857802575 1104 24 1.678799347555966 1104 25 1.9761623329713487 1105 1 0.766063454758374 1105 2 1.0219720795028344 1105 3 0.9097123597990127 1105 4 1.2544472681038954 1105 5 1.1627136227763373 1105 6 0.852389843309517 1105 7 0.8068727848762478 1105 8 0.8522753576118802 1105 9 1.1147315483096705 1105 10 1.49264363578758 1105 11 0.9030132704085716 1105 12 0.8884215541882128 1105 13 0.8371484502574305 1105 14 1.6480470722396987 1105 15 1.3173892014971145 1105 16 1.4906857543099292 1105 17 1.7372335117303592 1105 18 1.4661073344103883 1105 19 1.2820273651457796 1105 20 1.0134041370571794 1105 21 0.9909140287213702 1105 22 1.245863677808181 1105 23 1.5013482563967102 1105 24 1.2577931996528946 1105 25 1.6285498136367451 1106 1 0.6668269094614274 1106 2 0.8727574180267368 1106 3 0.8502097380146978 1106 4 1.2944305474970292 1106 5 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1.6122552196342497 1107 18 1.7037922980831368 1107 19 1.0560853781900832 1107 20 0.8792546914272918 1107 21 0.7754047200490106 1107 22 0.9602854226218867 1107 23 1.3355997568147089 1107 24 1.0771528653894435 1107 25 1.3589033739455942 1108 1 0.6779417217196269 1108 2 0.8647009015107017 1108 3 0.651409394610674 1108 4 1.182639796529827 1108 5 1.1191175404354397 1108 6 0.711564615632625 1108 7 0.6174796313492763 1108 8 0.6650778041280176 1108 9 0.8660753808257056 1108 10 1.1235539728571453 1108 11 0.7394287739921239 1108 12 0.6087541779959409 1108 13 0.6993678957496954 1108 14 1.6801409249637373 1108 15 1.1236610935055253 1108 16 1.2128052712038198 1108 17 1.4751817927025002 1108 18 1.739270763305143 1108 19 1.1441398765969808 1108 20 0.9987021378116125 1108 21 0.9387226386798773 1108 22 1.29670315213054 1108 23 1.502572019666637 1108 24 1.1838601824626167 1108 25 1.495301073269225 1109 1 0.3770930803589678 1109 2 0.5743160881317948 1109 3 0.4422775568211179 1109 4 0.7100256289866067 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-9.263512847279337 2215 17 -4.442271839394355 2215 18 -2.995066319017049 2215 19 -3.4378677269391105 2215 20 -3.1645655493267135 2215 21 -3.2021182004091635 2215 22 -9.064861072915463 2215 23 -10.285029399704548 2215 24 -6.40423883351114 2215 25 -4.728549479412177 2216 1 -1.8878952191173113 2216 2 -1.9087027781429775 2216 3 -1.685599083022801 2216 4 -6.959090001449972 2216 5 -3.498396889578988 2216 6 -2.6660057306877185 2216 7 -2.1872191420994973 2216 8 -7.893724504674289 2216 9 -3.9778099005595777 2216 10 -2.2708589059307336 2216 11 -2.238475266411914 2216 12 -2.441119559377796 2216 13 -2.160706058074254 2216 14 -1.5697012467534321 2216 15 -2.2059745486301168 2216 16 -1.9710646782911283 2216 17 -1.608737759967049 2216 18 -7.392718774934391 2216 19 -4.6522841854520784 2216 20 -4.0815648174553445 2216 21 -3.376550629718391 2216 22 -2.973791977739591 2216 23 -2.1748946109249943 2216 24 -2.568304319701884 2216 25 -1.967654374061219 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 150134.0 3 5.0 4 1357.0 5 7467.0 6 8643.0 7 7872.0 8 7119.0 9 6710.0 10 6496.0 11 6146.0 12 6000.0 13 5965.0 14 6008.0 15 6068.0 16 6139.0 17 6671.0 18 7358.0 19 9136.0 20 11642.0 21 15232.0 22 18552.0 23 22252.0 24 25620.0 25 29315.0 26 33181.0 27 37423.0 28 41739.0 29 49736.0 30 59765.0 31 71879.0 32 90962.0 33 118879.0 34 156549.0 35 210321.0 36 299168.0 37 436124.0 38 1220867.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.942917032042054 18.91389333070732 12.721097048392114 25.422092588858515 2 13.819347668694343 19.970739393499464 42.66989267564968 23.54002026215651 3 18.46875936699601 24.57996747247081 29.64394933515057 27.30732382538261 4 10.97950423151998 16.84619614664253 39.90479785455008 32.26950176728741 5 12.292202264128276 38.30158153580646 36.80655478995819 12.599661410107075 6 31.59196945691895 37.74072464908043 18.325505295474095 12.34180059852653 7 27.882654414817083 31.465945871711398 22.551664081251737 18.099735632219787 8 24.37460829136381 37.807343023154196 20.781219958600612 17.036828726881385 9 26.037626311887944 15.201810307830362 20.50776013421248 38.25280324606921 10 13.362650574957325 29.423188629263407 36.15709205590981 21.05706873986946 11 34.231669720841836 22.503917990168585 24.93904915115155 18.32536313783803 12 22.79135304607753 24.872369309450463 32.40066812774088 19.93560951673113 13 28.682727571539118 22.016876654585456 25.93509732965788 23.365298444217547 14 19.80137723685496 22.04112732277229 29.067214040436863 29.090281399935886 15 22.648564307103662 31.282452004125883 23.421355947442425 22.647627741328023 16 21.58507793264981 28.417512747394337 28.463926497100932 21.533482822854914 17 21.10595544971586 28.70886844176404 28.701656829007042 21.483519279513057 18 20.925506590024966 27.88038500654601 30.392279890793695 20.80182851263533 19 23.04905076132625 27.372566034673273 27.789126051154632 21.789257152845842 20 23.119926281044776 27.780271563949384 28.210330018435982 20.889472136569857 21 22.662297100334055 27.160405185915202 27.300039427240506 22.877258286510234 22 22.5576883691656 28.38512527973146 27.61386568893862 21.44332066216432 23 21.69962656924108 28.014018246695294 28.170969668232537 22.115385515831086 24 22.59298655651033 27.354827389514018 28.472074138852054 21.580111915123588 25 22.358987134233466 27.607219663928557 28.152031634807408 21.881761567030573 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 347.0 3 518.0 4 518.0 5 518.0 6 1022.0 7 1526.0 8 1526.0 9 1526.0 10 2856.5 11 4187.0 12 4187.0 13 4187.0 14 8679.5 15 13172.0 16 13172.0 17 13172.0 18 25364.0 19 37556.0 20 37556.0 21 37556.0 22 60513.5 23 83471.0 24 83471.0 25 83471.0 26 120613.5 27 157756.0 28 157756.0 29 157756.0 30 204773.0 31 251790.0 32 251790.0 33 251790.0 34 290251.0 35 328712.0 36 328712.0 37 328712.0 38 356607.5 39 384503.0 40 384503.0 41 384503.0 42 401985.0 43 419467.0 44 419467.0 45 419467.0 46 425161.0 47 430855.0 48 430855.0 49 430855.0 50 407848.0 51 384841.0 52 384841.0 53 384841.0 54 336428.0 55 288015.0 56 288015.0 57 288015.0 58 242594.0 59 197173.0 60 197173.0 61 197173.0 62 157312.5 63 117452.0 64 117452.0 65 117452.0 66 89121.5 67 60791.0 68 60791.0 69 60791.0 70 43765.5 71 26740.0 72 26740.0 73 26740.0 74 18481.0 75 10222.0 76 10222.0 77 10222.0 78 7017.5 79 3813.0 80 3813.0 81 3813.0 82 2530.5 83 1248.0 84 1248.0 85 1248.0 86 801.5 87 355.0 88 355.0 89 355.0 90 235.0 91 115.0 92 115.0 93 115.0 94 73.0 95 31.0 96 31.0 97 31.0 98 23.0 99 15.0 100 15.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17896707754719926 2 0.2619441410516461 3 0.2633172101731939 4 0.4639101263847714 5 0.259104384459354 6 0.019722265564050552 7 0.019659853331252926 8 0.01834919644250273 9 0.019503822749258853 10 0.021750663129973476 11 0.02150101419878296 12 0.02874083320330785 13 0.028179123108129193 14 0.02618193165860509 15 0.04081760024964893 16 0.02209393041036043 17 0.041597753159619284 18 0.03189265095958808 19 0.027461382430956468 20 0.03064440630363551 21 0.03529411764705882 22 0.04456233421750663 23 0.029989077859260416 24 0.04796380090497737 25 0.0372288968637853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3204500.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.10413472298092 #Duplication Level Percentage of deduplicated Percentage of total 1 82.61864087561001 59.571456123245625 2 11.98797209396559 17.287647098372613 3 2.955003424106453 6.3920389499592485 4 1.0553088645150401 3.043685301653939 5 0.46515807303099277 1.676991018265445 6 0.244654397432083 1.058435617980758 7 0.148479687900575 0.7494199594006453 8 0.09709466240520977 0.5600741295158077 9 0.06336726577510075 0.4112137681627323 >10 0.329012843153026 4.207780297707355 >50 0.020644097829217202 1.0067571787217269 >100 0.011821543347681488 1.6052964207641038 >500 0.0019239310921057946 0.9771801462324536 >1k 8.745141304916569E-4 1.2839795243396879 >5k 4.372570652458285E-5 0.16804446567785233 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 5330 0.1663286004056795 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4168 0.13006709315025747 No Hit CTGTAGGACGTGGAATATGGCAAGA 4033 0.12585426743641756 No Hit CTTTAGGACGTGAAATATGGCGAGG 3449 0.10762989545951007 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 3.120611639881417E-5 0.0 6 0.0 0.0 0.0 3.120611639881417E-5 0.0 7 0.0 0.0 0.0 6.241223279762834E-5 0.0 8 0.0 0.0 0.0 6.241223279762834E-5 0.0 9 0.0 0.0 0.0 9.36183491964425E-5 0.0 10 0.0 0.0 0.0 1.2482446559525668E-4 0.0 11 0.0 0.0 0.0 1.5603058199407084E-4 0.0 12 0.0 0.0 0.0 1.5603058199407084E-4 0.0 13 0.0 0.0 0.0 1.5603058199407084E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCG 35 0.0021879924 16.266575 9 TAGGACC 1175 0.0 13.706627 4 TCCAACG 300 0.0 12.651584 18 GGCGAGG 1040 0.0 11.861046 19 GTAGGAC 2810 0.0 11.632588 3 AGGACCT 2055 0.0 11.56979 5 TGTAGGA 3040 0.0 11.293559 2 AGGACGT 2385 0.0 11.125336 5 GGACGTG 2375 0.0 11.063524 6 GGACCTG 2015 0.0 10.874624 6 GCACCGT 115 8.996449E-7 10.723162 6 CTGTAGG 3045 0.0 10.697234 1 GACGTGG 1190 0.0 10.523131 7 GTCCTAA 1325 0.0 10.453377 1 TTAGGAC 1715 0.0 10.446775 3 GACGTGA 1290 0.0 10.442793 7 GTCCTAC 2925 0.0 10.384981 1 TGGCGAG 2545 0.0 10.290286 18 TAGGACG 2580 0.0 10.194621 4 TAGAACG 75 0.0025810408 10.1650715 4 >>END_MODULE