FastQCFastQC Report
Thu 26 May 2016
SRR1513181_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513181_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3204500
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT54270.1693555936963645No Hit
CTGTAGGACGTGGAATATGGCAAGA46350.14464034950850368No Hit
CTTTAGGACGTGAAATATGGCGAGG42940.13399906381650803No Hit
GTCCTAAAGTGTGTATTTCTCATTT42870.13378062100171634No Hit
GTCCTACAGTGTGCATTTCTCATTT33310.10394757372444999No Hit
GTATCAACGCAGAGTACTTTTTTTT32330.1008893743173662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11400.012.4427874
GGCGAGG11450.012.34708519
GCCGGTT1552.5465852E-1111.63305111
TGTAGGA29350.011.2237572
GTAGGAC28450.011.1765373
TTCTACG600.005783361411.1075182
GGACCGT1201.241624E-711.105786
TGGCGAG26150.011.07016618
AGGACGT27350.010.92970855
CGGTTTC1752.1827873E-1110.84754613
GGACGTG26600.010.8075576
TCCAACG2150.010.59494718
TAGGACG28500.010.5888954
CTGTAGG30150.010.5192211
GACGTGA15200.010.4897357
TTAGGAC17950.010.4482813
GACGTGG12400.010.2560947
GTCCTAC31400.010.2217891
CTAGGAC5850.010.0897143
ATGGCGA29700.010.0030917
Produced by FastQC (version 0.11.5)