##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513181_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3204500
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.83234264315806	31.0	31.0	34.0	27.0	34.0
2	30.943771259166798	33.0	31.0	34.0	27.0	34.0
3	31.054335153690122	33.0	31.0	34.0	27.0	34.0
4	34.44471025120924	37.0	35.0	37.0	31.0	37.0
5	34.19736776408176	37.0	35.0	37.0	30.0	37.0
6	34.12498486503355	37.0	35.0	37.0	30.0	37.0
7	34.00161148385084	37.0	35.0	37.0	30.0	37.0
8	33.97314588859417	37.0	35.0	37.0	30.0	37.0
9	35.541456389452335	39.0	37.0	39.0	30.0	39.0
10	35.38577562802309	39.0	35.0	39.0	30.0	39.0
11	35.40821501014199	39.0	35.0	39.0	30.0	39.0
12	35.342184116086756	39.0	35.0	39.0	29.0	39.0
13	35.31810578873459	39.0	35.0	39.0	29.0	39.0
14	36.49563738492745	40.0	37.0	41.0	29.0	41.0
15	36.48552067405211	40.0	37.0	41.0	29.0	41.0
16	36.448251521298175	40.0	37.0	41.0	29.0	41.0
17	36.38772351380871	40.0	37.0	41.0	29.0	41.0
18	36.34639631767826	40.0	37.0	41.0	29.0	41.0
19	36.427169605242625	40.0	37.0	41.0	29.0	41.0
20	36.38712622874083	40.0	37.0	41.0	29.0	41.0
21	36.33538305507879	40.0	37.0	41.0	29.0	41.0
22	36.28182899048213	40.0	37.0	41.0	28.0	41.0
23	36.20819316586051	40.0	37.0	41.0	28.0	41.0
24	36.153569043532535	40.0	37.0	41.0	28.0	41.0
25	36.09920830082696	40.0	37.0	41.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4855679635011825
1101	2	0.595953778177531
1101	3	0.5306326144354294
1101	4	0.657360309092283
1101	5	0.49480654972715143
1101	6	0.5126652393425033
1101	7	0.5548843980334652
1101	8	0.5413773841814162
1101	9	0.6580483085878797
1101	10	0.7765997391480184
1101	11	0.6873699318937199
1101	12	0.70762755452769
1101	13	0.6808595832250006
1101	14	0.7498409799342909
1101	15	0.8218144241220102
1101	16	0.6754132632120573
1101	17	0.7413710564758986
1101	18	0.7639900519977232
1101	19	0.7257425836117193
1101	20	0.7565980250447666
1101	21	0.7507876951010175
1101	22	0.7515360511560161
1101	23	0.8346207487471062
1101	24	0.8497409519991379
1101	25	0.8744126272044497
1102	1	0.48664831315926094
1102	2	0.47891086698381713
1102	3	0.4814444490441758
1102	4	0.507799268527954
1102	5	0.5479015484378067
1102	6	0.5591938613306411
1102	7	0.5533526447923762
1102	8	0.6263311072277062
1102	9	0.5952132712599294
1102	10	0.6954036095024634
1102	11	0.7309731459878677
1102	12	0.6982531132788878
1102	13	0.6882733300095154
1102	14	0.7374890181167046
1102	15	0.6634101321939951
1102	16	0.7537660175016967
1102	17	0.7571119594447921
1102	18	0.7883402383903828
1102	19	0.7897564455832153
1102	20	0.8241967897946765
1102	21	0.7463927996273583
1102	22	0.756737974293415
1102	23	0.7818997034027859
1102	24	0.7567832612016119
1102	25	0.7280152752471238
1103	1	0.25911283223698334
1103	2	0.307865850522159
1103	3	0.3619580422033515
1103	4	0.4146273365216544
1103	5	0.33959604979168034
1103	6	0.2020401474260467
1103	7	0.359986999306642
1103	8	0.3574889504978245
1103	9	0.47387024428087443
1103	10	0.3318966780674728
1103	11	0.4491915134269959
1103	12	0.46365652359273923
1103	13	0.4518953203424445
1103	14	0.4800778167476949
1103	15	0.5164552071151363
1103	16	0.521955553785105
1103	17	0.5909552189331677
1103	18	0.47358958138547536
1103	19	0.476129007889071
1103	20	0.4676622149944336
1103	21	0.4210995057276108
1103	22	0.3426646501541697
1103	23	0.36592936893480754
1103	24	0.4313597620824865
1103	25	0.4545667767258834
1104	1	0.3884336767056169
1104	2	0.4842765779024347
1104	3	0.4043703769000935
1104	4	0.5501615014482866
1104	5	0.5765380475111925
1104	6	0.4375637316840084
1104	7	0.46562224243754
1104	8	0.48645010474113093
1104	9	0.3786675929700962
1104	10	0.5471942508410734
1104	11	0.5773398202705877
1104	12	0.5884396264486469
1104	13	0.5951050646394478
1104	14	0.6989086239792002
1104	15	0.5995369542014544
1104	16	0.6143200654347396
1104	17	0.6013494788022626
1104	18	0.5608583064779111
1104	19	0.676567042321139
1104	20	0.6821015196318356
1104	21	0.5990504686603515
1104	22	0.6539308568899287
1104	23	0.6668522500377634
1104	24	0.6874934649778339
1104	25	0.6265338858115257
1105	1	0.3555068378837376
1105	2	0.41954789775616774
1105	3	0.28856270190072664
1105	4	0.38511776629469097
1105	5	0.4379434826996871
1105	6	0.4224930129547104
1105	7	0.4470805654344261
1105	8	0.4791832537157106
1105	9	0.5466580651565565
1105	10	0.5543846774165857
1105	11	0.438810712391124
1105	12	0.4119901079313877
1105	13	0.5732822718741772
1105	14	0.5812816901925402
1105	15	0.5589762006807462
1105	16	0.4322610532251048
1105	17	0.42735228857588936
1105	18	0.473756629753332
1105	19	0.6002822977032736
1105	20	0.5242240999232095
1105	21	0.5645557970461113
1105	22	0.5459460686631274
1105	23	0.4711315840134773
1105	24	0.5393686494018723
1105	25	0.6026538565299973
1106	1	0.32720216722669093
1106	2	0.3246676005465261
1106	3	0.3001855752805973
1106	4	0.42239121318453954
1106	5	0.45450585928263365
1106	6	0.36120284203865083
1106	7	0.2802071137574913
1106	8	0.39818490696999476
1106	9	0.525914852987782
1106	10	0.5029017921031951
1106	11	0.44963422792435637
1106	12	0.4513912434965306
1106	13	0.42409197324550973
1106	14	0.5608055290250391
1106	15	0.49850863917097143
1106	16	0.5640964561182145
1106	17	0.5155537462435191
1106	18	0.5047162523941964
1106	19	0.5155978948836832
1106	20	0.545200938976258
1106	21	0.5197616673810543
1106	22	0.5315095855983003
1106	23	0.3250469927878612
1106	24	0.5068579254748187
1106	25	0.5887208723119883
1107	1	0.23661156395592897
1107	2	0.3121947227041382
1107	3	0.28628781562802885
1107	4	0.32737686809348787
1107	5	0.26178173776691693
1107	6	0.21156715490781863
1107	7	0.22346181320729386
1107	8	0.188279907254163
1107	9	0.2833533525215799
1107	10	0.34955740089318255
1107	11	0.30079245478953
1107	12	0.30404208103314545
1107	13	0.34529429903096087
1107	14	0.2996359461748668
1107	15	0.3692611971811175
1107	16	0.3813936725718037
1107	17	0.36128078558951415
1107	18	0.41921557438776347
1107	19	0.32997360748293403
1107	20	0.36822825355308453
1107	21	0.4465385344787052
1107	22	0.46360996652669684
1107	23	0.42561489337811054
1107	24	0.38189534735967356
1107	25	0.4509821565778722
1108	1	0.21806763030708254
1108	2	0.19149160498254858
1108	3	0.1883614638234512
1108	4	0.28970818365741025
1108	5	0.27446305752320654
1108	6	0.21474694678021677
1108	7	0.16143549214671538
1108	8	0.2358443182735641
1108	9	0.2417601492757555
1108	10	0.28118605290831766
1108	11	0.2542711855279407
1108	12	0.273438112727419
1108	13	0.33062131509237247
1108	14	0.32182636336169423
1108	15	0.39525967861360556
1108	16	0.3337384269924186
1108	17	0.31908755020222657
1108	18	0.403330880709845
1108	19	0.334786965753068
1108	20	0.43655418425796455
1108	21	0.4047217384698314
1108	22	0.2433530444674048
1108	23	0.33550671873352655
1108	24	0.3927326090936347
1108	25	0.3278847330186494
1109	1	0.1859201581317116
1109	2	0.1837671635736271
1109	3	0.10625064959728192
1109	4	0.15018136466242993
1109	5	0.1258353206367815
1109	6	0.1705663306381311
1109	7	0.19747270717870435
1109	8	0.19369878392001283
1109	9	0.11334055773318141
1109	10	0.2924374284011293
1109	11	0.2362930517651165
1109	12	0.2554154270965512
1109	13	0.39872316943375097
1109	14	0.34795236571724786
1109	15	0.2869470787715116
1109	16	0.25454713315698285
1109	17	0.29396873975596094
1109	18	0.344325968610228
1109	19	0.35950735052382043
1109	20	0.3642304358744539
1109	21	0.2839563791825839
1109	22	0.3235323897131508
1109	23	0.2839002857552799
1109	24	0.22797152186738856
1109	25	0.33582943361697914
1110	1	0.22179104505023162
1110	2	0.243173311433992
1110	3	0.24648179743720533
1110	4	0.21505964141018552
1110	5	0.20388330832503243
1110	6	0.12809971243562046
1110	7	0.21125984542501897
1110	8	0.20802394785450673
1110	9	0.2657905596548531
1110	10	0.27653429019733977
1110	11	0.21908268981312773
1110	12	0.17618618702154976
1110	13	0.20537107191449877
1110	14	0.33645581285956894
1110	15	0.30668312358967853
1110	16	0.294177494503856
1110	17	0.3297075223353616
1110	18	0.3569869286093166
1110	19	0.3519554880202733
1110	20	0.2887905991588511
1110	21	0.27074962505692923
1110	22	0.26313490734137446
1110	23	0.2880323181341282
1110	24	0.33045672708090734
1110	25	0.33253189506712744
1111	1	0.12483894055095845
1111	2	0.1567169673808273
1111	3	0.1387627538194529
1111	4	0.1816359151860638
1111	5	0.09263512254854334
1111	6	0.12279072152583126
1111	7	0.14859465559994334
1111	8	0.15109934316426177
1111	9	0.1873740903247736
1111	10	0.2585142764474355
1111	11	0.2983967223545747
1111	12	0.30636722743098943
1111	13	0.25336587750612694
1111	14	0.28334298725418705
1111	15	0.2301687243841215
1111	16	0.25487186468183864
1111	17	0.30476290389520955
1111	18	0.3185826814165864
1111	19	0.22854627558425022
1111	20	0.2691778881360065
1111	21	0.26377536793371803
1111	22	0.23700244992788555
1111	23	0.24272256334076303
1111	24	0.2393597902540776
1111	25	0.27545833307797807
1112	1	-0.3459518719526038
1112	2	-0.26195179164181326
1112	3	-0.20820753471249276
1112	4	-1.0162629820198035
1112	5	-0.3908177253058014
1112	6	-0.20744640928406
1112	7	-0.10793750256294743
1112	8	-0.08560360031295033
1112	9	-0.08025885523063181
1112	10	0.024165630421549622
1112	11	-0.039341607834998626
1112	12	0.022907789687479863
1112	13	-0.03682846666195161
1112	14	-0.033123815881410223
1112	15	-0.04528622111928371
1112	16	-0.07560472523313422
1112	17	-0.0833426637473238
1112	18	-0.11872978711024018
1112	19	-0.140812049837578
1112	20	-0.14353789847940845
1112	21	0.006133091333438756
1112	22	-0.01813705822422662
1112	23	-0.0756010403123426
1112	24	-0.07728894939984343
1112	25	-0.07837528532099469
1113	1	-0.4799290782951964
1113	2	-0.3407309490178534
1113	3	-0.3855009593751113
1113	4	-1.3684680798459254
1113	5	-0.5929103398048596
1113	6	-0.4266002099490578
1113	7	-0.2843611820340257
1113	8	-0.13526861995401163
1113	9	-0.13641308616755765
1113	10	-0.10539182840726369
1113	11	-0.06950995561071238
1113	12	-0.16252917451900828
1113	13	-0.12414582515080497
1113	14	-0.07952455256175739
1113	15	-0.0972196684641915
1113	16	-0.09765506617245023
1113	17	-0.17553780501250316
1113	18	-0.19611978196944335
1113	19	-0.16280296051237286
1113	20	-0.1253511222231225
1113	21	-0.12301178124879186
1113	22	-0.1889072147034767
1113	23	-0.2075236802716134
1113	24	-0.1939197282063745
1113	25	-0.15354664324757294
1114	1	-0.5655560525047676
1114	2	-0.5642090691456154
1114	3	-0.43031393081946945
1114	4	-1.252787101553622
1114	5	-0.7497282390212945
1114	6	-0.6339556678254183
1114	7	-0.6086778649884366
1114	8	-0.5704730977524832
1114	9	-0.5517628251302256
1114	10	-0.4296575284312638
1114	11	-0.5270332158832218
1114	12	-0.5117402629202559
1114	13	-0.5588229014759563
1114	14	-0.6746967606080503
1114	15	-0.5380120960727623
1114	16	-0.5324476617804166
1114	17	-0.591250098013532
1114	18	-0.5843797763179808
1114	19	-0.667492838517397
1114	20	-0.5663747459952546
1114	21	-0.6361193586991121
1114	22	-0.6724253867692127
1114	23	-0.6343769649513078
1114	24	-0.5904552686671991
1114	25	-0.5901921177205693
1115	1	-0.2111082005824585
1115	2	-0.16497702719172835
1115	3	-0.17575711249270753
1115	4	0.09837011983950816
1115	5	-0.16450758205227345
1115	6	-0.14493134535774743
1115	7	-0.2417095818652939
1115	8	-0.2915676179085338
1115	9	-0.2944843521905227
1115	10	-0.3133210864576981
1115	11	-0.23972406653182787
1115	12	-0.1707321323500821
1115	13	-0.21882403122797456
1115	14	-0.28167698812552544
1115	15	-0.31337912881552654
1115	16	-0.4314414536108515
1115	17	-0.434983983103173
1115	18	-0.46774814107482143
1115	19	-0.472888725491174
1115	20	-0.4571124649530418
1115	21	-0.45834513417365486
1115	22	-0.42878160851402214
1115	23	-0.547791266883003
1115	24	-0.4964763002765693
1115	25	-0.5303469210799392
1116	1	-0.2414184521647087
1116	2	-0.17368344893294463
1116	3	-0.20450469450838327
1116	4	-0.037658629700388246
1116	5	-0.3075118457599686
1116	6	-0.42541235222375917
1116	7	-0.36784011889555757
1116	8	-0.4615944297046042
1116	9	-0.42586900552792173
1116	10	-0.4007233182194625
1116	11	-0.31966634816924255
1116	12	-0.3827804070084895
1116	13	-0.42495069165179444
1116	14	-0.5125389708670767
1116	15	-0.5961286217240769
1116	16	-0.5177286715499747
1116	17	-0.3854380998578364
1116	18	-0.5630090316538272
1116	19	-0.45032091844212374
1116	20	-0.5357235266509406
1116	21	-0.5573404050494304
1116	22	-0.5454392129838084
1116	23	-0.6820836547495048
1116	24	-0.614225563833223
1116	25	-0.5965548000447924
1201	1	0.3245458711029805
1201	2	0.38657418868325166
1201	3	0.3519855404349137
1201	4	0.41364516983637145
1201	5	0.4452936604065556
1201	6	0.3199201565979237
1201	7	0.34983095562819244
1201	8	0.4121910506696054
1201	9	0.4505148406852655
1201	10	0.5298884686118797
1201	11	0.4803226569922572
1201	12	0.4906336179103121
1201	13	0.4893156883796337
1201	14	0.5278388142135952
1201	15	0.686387701966396
1201	16	0.6758445719942046
1201	17	0.6589354889607222
1201	18	0.6177450502558983
1201	19	0.5945998306404761
1201	20	0.5826078152599905
1201	21	0.6205358841865021
1201	22	0.5792691352573485
1201	23	0.6815782217717725
1201	24	0.5230644332327969
1201	25	0.6251060037204255
1202	1	0.4087987741632233
1202	2	0.4533813696296676
1202	3	0.3715927545915072
1202	4	0.459021407198577
1202	5	0.5392001586573656
1202	6	0.501296793991834
1202	7	0.4488218190551052
1202	8	0.4928125338814837
1202	9	0.5856708341790906
1202	10	0.5643798811389118
1202	11	0.5706166048145391
1202	12	0.6004300546090704
1202	13	0.5623261910987196
1202	14	0.626540032045952
1202	15	0.7253964536033166
1202	16	0.6265953862096723
1202	17	0.7090413464060248
1202	18	0.7050925381700353
1202	19	0.6530780496691548
1202	20	0.6903050290014505
1202	21	0.6785848239138019
1202	22	0.7175461307928472
1202	23	0.676082503243137
1202	24	0.6737971328052126
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2210	17	-0.23809578179550783
2210	18	-0.3053446211407902
2210	19	-0.32632588615592084
2210	20	-0.3727343566939538
2210	21	-0.4218648727111187
2210	22	-0.39123398680059296
2210	23	-0.326131555015472
2210	24	-0.28829736170687426
2210	25	-0.366181005282769
2211	1	-0.5903216023156439
2211	2	-0.46152823288398537
2211	3	-0.2922741544890535
2211	4	-1.2436744125199937
2211	5	-0.6694283498116036
2211	6	-0.4799918835718202
2211	7	-0.499119263632096
2211	8	-0.38727578096681725
2211	9	-0.44296147736803704
2211	10	-0.3816276467567974
2211	11	-0.43861709757260314
2211	12	-0.39126186889473047
2211	13	-0.45910929627738994
2211	14	-0.4522971153608424
2211	15	-0.3724111332789093
2211	16	-0.3033973342589107
2211	17	-0.40847360719383374
2211	18	-0.41647814281380136
2211	19	-0.4481336317312099
2211	20	-0.4938103607881885
2211	21	-0.38392406933871115
2211	22	-0.5175945378245146
2211	23	-0.48111681103858217
2211	24	-0.465084102496661
2211	25	-0.5677992802950769
2212	1	-0.11765297650592643
2212	2	-0.4087287916987421
2212	3	-0.3274997005307263
2212	4	-0.03346414586154367
2212	5	-0.2632145100120056
2212	6	-0.236351209672506
2212	7	-0.34930464693928087
2212	8	-0.3267749720050048
2212	9	-0.33496103680242584
2212	10	-0.36996205659212933
2212	11	-0.3881476113463975
2212	12	-0.4480176059105716
2212	13	-0.4755754738830049
2212	14	-0.46830895023962427
2212	15	-0.4654490264512532
2212	16	-0.4329738643411787
2212	17	-0.454389135153896
2212	18	-0.5009968739485942
2212	19	-0.4951745043832858
2212	20	-0.4517474417375169
2212	21	-0.453184366012394
2212	22	-0.4447503047951997
2212	23	-0.502340848165673
2212	24	-0.5278447730018954
2212	25	-0.5603907044543845
2213	1	-0.24646185924310515
2213	2	-0.27555626850158177
2213	3	-0.3029999686182805
2213	4	-0.15451565955235935
2213	5	-0.2883567799241149
2213	6	-0.34516048849972947
2213	7	-0.48048767161005657
2213	8	-0.4648880826037072
2213	9	-0.5882059318285684
2213	10	-0.41595610543003403
2213	11	-0.5249108906740005
2213	12	-0.5925749925447903
2213	13	-0.5623118899476225
2213	14	-0.6076879137519171
2213	15	-0.6053842966164638
2213	16	-0.5356224894090644
2213	17	-0.5164821367333516
2213	18	-0.6700728885341576
2213	19	-0.5422855271513782
2213	20	-0.5712178657119367
2213	21	-0.5913921021597375
2213	22	-0.6926466760107886
2213	23	-0.5576011874331499
2213	24	-0.5699211278978495
2213	25	-0.6015438061574017
2214	1	-0.44516373325627967
2214	2	-0.4627489075263007
2214	3	-0.5535064823815539
2214	4	-0.31675602327580776
2214	5	-0.46884716771155865
2214	6	-0.6216041728234316
2214	7	-0.5885471319136926
2214	8	-0.6189265520169087
2214	9	-0.6674822004440415
2214	10	-0.8139938310310555
2214	11	-0.7695085292298529
2214	12	-0.8389943682495371
2214	13	-0.7912965278151418
2214	14	-0.91756815396284
2214	15	-0.8494843468007431
2214	16	-0.8818209018415715
2214	17	-0.8875792898436146
2214	18	-0.9897129869598658
2214	19	-0.8792172735199344
2214	20	-0.9530281833229282
2214	21	-0.8757150903702424
2214	22	-0.955815298197983
2214	23	-0.9441155321418861
2214	24	-1.0253694546661052
2214	25	-0.9731988184074396
2215	1	-0.5031711860613548
2215	2	-0.4697931126099206
2215	3	-0.5206470264029548
2215	4	-0.3622062840617275
2215	5	-0.5309292529384066
2215	6	-0.6057374328032381
2215	7	-0.591368332621947
2215	8	-0.6474796536990226
2215	9	-0.8082882686820554
2215	10	-0.8685758574092901
2215	11	-0.7752162674708032
2215	12	-0.7942166080651347
2215	13	-0.8300225520774163
2215	14	-0.9116748633376801
2215	15	-0.8092974429096387
2215	16	-0.9052308621886311
2215	17	-0.9465853677422515
2215	18	-0.9816931365972792
2215	19	-0.8967704591115933
2215	20	-0.9104288186221154
2215	21	-0.9291209140008974
2215	22	-1.065107681151943
2215	23	-0.9480879271236944
2215	24	-0.8871781885276349
2215	25	-0.9473230225214948
2216	1	-0.5216735313090801
2216	2	-0.48092452055857393
2216	3	-0.5254056718186852
2216	4	-0.35835729253306425
2216	5	-0.5066967548866117
2216	6	-0.7174770791938059
2216	7	-0.8037295511433271
2216	8	-0.7769397726355294
2216	9	-0.9236213442307388
2216	10	-0.8168704045231436
2216	11	-0.8247331434763865
2216	12	-0.7875512451369389
2216	13	-1.0032756906796365
2216	14	-1.1317270276916958
2216	15	-1.0564899606360356
2216	16	-1.0113418399628529
2216	17	-1.1901566777919967
2216	18	-1.1612208869669232
2216	19	-1.1400732434821421
2216	20	-1.0661288965501186
2216	21	-1.3005226698995713
2216	22	-1.2108767535123945
2216	23	-1.2220104169086028
2216	24	-1.2486815323205107
2216	25	-1.3281172511083312
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	73934.0
3	0.0
4	1255.0
5	10150.0
6	11275.0
7	8644.0
8	6940.0
9	5941.0
10	5485.0
11	4917.0
12	4512.0
13	4329.0
14	4202.0
15	4256.0
16	4429.0
17	4601.0
18	5280.0
19	6213.0
20	7727.0
21	10381.0
22	13043.0
23	15796.0
24	18451.0
25	20854.0
26	22957.0
27	25232.0
28	28951.0
29	34881.0
30	44225.0
31	56807.0
32	72854.0
33	95633.0
34	127422.0
35	171230.0
36	247874.0
37	407279.0
38	1616540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.23042771385693	19.74615432716358	13.700318909454728	27.323099049524764
2	13.31554203436253	20.35735730862234	40.900596344534094	25.42650431248104
3	16.926207317320554	24.56659223578206	30.05172712420595	28.455473322691432
4	10.9442792040876	17.39530018352758	38.31981607921301	33.340604533171806
5	12.285776215319421	38.48026355241065	36.581358189413926	12.652602042856001
6	31.67094545785884	38.20890082504112	17.872164811584458	12.247988905515586
7	27.628580980662633	31.514096982444446	22.58295413716366	18.274367899729263
8	24.437300641069655	38.18818234592793	20.360609484335303	17.01390752866711
9	25.83630535163023	15.105113951987498	20.063588916504283	38.99499177987799
10	13.774091701616323	29.74468312189818	35.391064268151226	21.090160908334273
11	34.184147292869405	22.47764081760025	24.845810578873458	18.492401310656888
12	22.602278046497112	24.95178655016383	32.374567015134964	20.07136838820409
13	28.580652207832735	21.90660009361835	26.01076611015759	23.501981588391324
14	20.1935940697227	22.18005846268783	28.000612900175597	29.62573456741387
15	22.55106880948666	31.34036511156187	23.324044312685285	22.784521766266188
16	21.74161335621782	28.643844593540337	27.89680137306912	21.717740677172724
17	21.249383164583392	29.070559729176086	28.068922013588015	21.611135092652503
18	21.14700218601171	28.17673263200742	29.665518045443946	21.010747136536924
19	22.98467779684818	27.43204868154158	27.69308784521766	21.890185676392573
20	23.039694180059293	27.823498205648306	28.138836011858327	20.997971602434077
21	22.40662799772942	27.134216080240492	27.43348581839039	23.0256701036397
22	22.48716256383611	28.471631255470832	27.42185586184406	21.619350318848994
23	21.722329698958685	28.05660873741656	27.801265232993927	22.419796330630827
24	22.449274457793727	27.4158527071306	28.44287720393197	21.691995631143705
25	22.281808697975	27.696050514075083	27.96208323750434	22.060057550445578
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	90.0
1	90.0
2	233.0
3	376.0
4	376.0
5	376.0
6	953.0
7	1530.0
8	1530.0
9	1530.0
10	2742.0
11	3954.0
12	3954.0
13	3954.0
14	8386.0
15	12818.0
16	12818.0
17	12818.0
18	24707.5
19	36597.0
20	36597.0
21	36597.0
22	59185.5
23	81774.0
24	81774.0
25	81774.0
26	118410.0
27	155046.0
28	155046.0
29	155046.0
30	201702.5
31	248359.0
32	248359.0
33	248359.0
34	285404.5
35	322450.0
36	322450.0
37	322450.0
38	351036.5
39	379623.0
40	379623.0
41	379623.0
42	398151.5
43	416680.0
44	416680.0
45	416680.0
46	425265.5
47	433851.0
48	433851.0
49	433851.0
50	412106.0
51	390361.0
52	390361.0
53	390361.0
54	341657.0
55	292953.0
56	292953.0
57	292953.0
58	247308.0
59	201663.0
60	201663.0
61	201663.0
62	161139.0
63	120615.0
64	120615.0
65	120615.0
66	91590.0
67	62565.0
68	62565.0
69	62565.0
70	44953.0
71	27341.0
72	27341.0
73	27341.0
74	18827.5
75	10314.0
76	10314.0
77	10314.0
78	7068.5
79	3823.0
80	3823.0
81	3823.0
82	2543.0
83	1263.0
84	1263.0
85	1263.0
86	807.5
87	352.0
88	352.0
89	352.0
90	223.0
91	94.0
92	94.0
93	94.0
94	50.5
95	7.0
96	7.0
97	7.0
98	4.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1903573100327664
2	0.00973630831643002
3	0.005773131533780621
4	0.0024028709627086906
5	0.08450616320798877
6	0.3383055078795444
7	0.03161179591199875
8	0.014978935871430801
9	0.00524262755500078
10	0.004025589015447028
11	0.0
12	0.0
13	0.0
14	0.0019659853331252925
15	0.0
16	0.0
17	0.020190357310032766
18	0.029614604462474645
19	0.0
20	0.0
21	0.0010298018411608676
22	1.5603058199407084E-4
23	0.005897955999375877
24	0.0
25	2.1844281479169918E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3204500.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.34465478741596
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.87677526738317	56.08372061236106
2	12.81565404840399	17.77394211723049
3	3.4327553641344593	7.141297070866634
4	1.2359040398147176	3.4281335596529754
5	0.5542261017798724	1.9216308851050257
6	0.2941873727855496	1.224019308517847
7	0.17793194612210483	0.8637040565643319
8	0.11626169117362191	0.644970147154878
9	0.08098477157524786	0.5054274925130969
>10	0.3751276564295422	4.59239525482563
>50	0.023215522498310145	1.1137844442208913
>100	0.013917708783629256	1.8934909360844818
>500	0.0017091668610779134	0.8509537966036455
>1k	0.0013043641796136583	1.7932627801183092
>5k	4.497807515909166E-5	0.16926753818074727
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	5427	0.1693555936963645	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4635	0.14464034950850368	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4294	0.13399906381650803	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4287	0.13378062100171634	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3331	0.10394757372444999	No Hit
GTATCAACGCAGAGTACTTTTTTTT	3233	0.1008893743173662	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	3.120611639881417E-5	0.0	0.0	0.0
6	0.0	3.120611639881417E-5	0.0	0.0	6.241223279762834E-5
7	0.0	3.120611639881417E-5	0.0	3.120611639881417E-5	6.241223279762834E-5
8	0.0	3.120611639881417E-5	0.0	6.241223279762834E-5	6.241223279762834E-5
9	0.0	3.120611639881417E-5	0.0	6.241223279762834E-5	6.241223279762834E-5
10	0.0	3.120611639881417E-5	0.0	6.241223279762834E-5	6.241223279762834E-5
11	0.0	3.120611639881417E-5	0.0	6.241223279762834E-5	6.241223279762834E-5
12	0.0	3.120611639881417E-5	0.0	6.241223279762834E-5	6.241223279762834E-5
13	0.0	3.120611639881417E-5	0.0	6.241223279762834E-5	6.241223279762834E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1140	0.0	12.442787	4
GGCGAGG	1145	0.0	12.347085	19
GCCGGTT	155	2.5465852E-11	11.633051	11
TGTAGGA	2935	0.0	11.223757	2
GTAGGAC	2845	0.0	11.176537	3
TTCTACG	60	0.0057833614	11.107518	2
GGACCGT	120	1.241624E-7	11.10578	6
TGGCGAG	2615	0.0	11.070166	18
AGGACGT	2735	0.0	10.9297085	5
CGGTTTC	175	2.1827873E-11	10.847546	13
GGACGTG	2660	0.0	10.807557	6
TCCAACG	215	0.0	10.594947	18
TAGGACG	2850	0.0	10.588895	4
CTGTAGG	3015	0.0	10.519221	1
GACGTGA	1520	0.0	10.489735	7
TTAGGAC	1795	0.0	10.448281	3
GACGTGG	1240	0.0	10.256094	7
GTCCTAC	3140	0.0	10.221789	1
CTAGGAC	585	0.0	10.089714	3
ATGGCGA	2970	0.0	10.00309	17
>>END_MODULE