FastQCFastQC Report
Thu 26 May 2016
SRR1513180_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513180_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835508
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29540.16093637292782162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGAC7350.013.0909653
TAGGACC4650.012.4972524
TGTAGGA9000.011.6515842
CTGTAGG9000.011.3537071
CTAGACA2200.011.2587014
GGACCGT855.391815E-511.1625576
GTCCTAC5500.010.9389171
TTAGACT1855.456968E-1210.81397154
TAGGACT2650.010.7848234
GTCCTAA3550.010.7603911
TAGGACG4350.010.7311054
AGGCGTG1252.2773747E-710.6273357
GGACGTG4600.010.5194976
TATGGCG5550.010.43043216
GTGCGCC1106.1225546E-610.3527111
TAAGCTG1951.4551915E-1110.2400775
ATATGGC10850.010.233438515
TCCTACA6450.010.19821552
GACTAGA1605.662514E-910.1467131
TGGCGAG5350.010.11109918
Produced by FastQC (version 0.11.5)