##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513180_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1835508
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.450578804341905	31.0	30.0	34.0	25.0	34.0
2	29.318292265683397	31.0	30.0	34.0	22.0	34.0
3	29.49341653645748	31.0	30.0	34.0	23.0	34.0
4	32.5826752049024	37.0	33.0	37.0	22.0	37.0
5	32.72728748662496	37.0	33.0	37.0	25.0	37.0
6	33.0824856116127	37.0	35.0	37.0	27.0	37.0
7	33.03313850988391	37.0	35.0	37.0	26.0	37.0
8	32.90599005833808	37.0	35.0	37.0	26.0	37.0
9	34.137525960115674	39.0	35.0	39.0	25.0	39.0
10	33.71242184724883	39.0	34.0	39.0	18.0	39.0
11	34.16080398451001	39.0	35.0	39.0	25.0	39.0
12	34.14873375654042	39.0	35.0	39.0	25.0	39.0
13	34.189979558792444	39.0	35.0	39.0	25.0	39.0
14	34.423369443227706	39.0	34.0	41.0	17.0	41.0
15	34.84551034373045	40.0	34.0	41.0	21.0	41.0
16	34.69022417772083	39.0	34.0	41.0	18.0	41.0
17	34.30040893311279	39.0	33.0	41.0	17.0	41.0
18	34.029214800480304	39.0	33.0	41.0	16.0	41.0
19	34.67453642261434	39.0	34.0	41.0	18.0	41.0
20	34.88473272794235	40.0	35.0	41.0	22.0	41.0
21	34.93742658708107	40.0	35.0	41.0	22.0	41.0
22	34.626777437090986	40.0	34.0	41.0	18.0	41.0
23	34.17962384255476	39.0	34.0	41.0	10.0	41.0
24	34.41769145108602	39.0	34.0	41.0	17.0	41.0
25	34.07166244985039	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.8462440366107487
1101	2	1.0471578014950111
1101	3	0.9689485463686438
1101	4	1.2814387305863164
1101	5	1.226983085930108
1101	6	0.8773499480819282
1101	7	0.9121143303391221
1101	8	0.9185757812352477
1101	9	1.2771356518245014
1101	10	1.6269108533228405
1101	11	1.1096449811501472
1101	12	0.9736260246157968
1101	13	0.9641716621797016
1101	14	1.9716708965323306
1101	15	1.5587502239154887
1101	16	1.6787205325359622
1101	17	2.0525013578807645
1101	18	2.0836727769060133
1101	19	1.683013965246822
1101	20	1.3447573373619477
1101	21	1.2465251912719069
1101	22	1.4494012089071404
1101	23	1.746828935914067
1101	24	1.445808467118482
1101	25	1.6629920374152007
1102	1	0.9415248372618805
1102	2	1.2384423444322543
1102	3	1.0372996365409435
1102	4	1.3261060805395957
1102	5	1.2935362423266596
1102	6	1.0490547768798777
1102	7	1.0747239827672956
1102	8	1.0197773823652554
1102	9	1.314648947921583
1102	10	1.5200393011569204
1102	11	1.2552986108231607
1102	12	1.0463196093297
1102	13	1.012467716199538
1102	14	1.9534041046921473
1102	15	1.5925271552408802
1102	16	1.6872133472605029
1102	17	1.9800839228755294
1102	18	2.235755903889661
1102	19	1.6515775776510537
1102	20	1.3936336825824611
1102	21	1.3343569477347756
1102	22	1.5499283157194768
1102	23	1.9310756985566613
1102	24	1.6690504754643456
1102	25	2.00039907239632
1103	1	0.6601801527848892
1103	2	0.9090414694152216
1103	3	0.7111514199882834
1103	4	1.2242107149291073
1103	5	1.0127597998786015
1103	6	0.6327155747760287
1103	7	0.5952947767490144
1103	8	0.6562592775578509
1103	9	0.9935321874310006
1103	10	1.3302529515091166
1103	11	0.8228502067668515
1103	12	0.8087717331815938
1103	13	0.6848629401550212
1103	14	1.6262015856119802
1103	15	1.2359669064471106
1103	16	1.2671781389627625
1103	17	1.5735962317223695
1103	18	1.7229896519513659
1103	19	1.1992255638791605
1103	20	0.9039761200433958
1103	21	0.9329391000690563
1103	22	1.1324450602062868
1103	23	1.5141471683733343
1103	24	1.0913110730970814
1103	25	1.3745218386266345
1104	1	0.6800854212658756
1104	2	0.9172605804004235
1104	3	0.8058483876794895
1104	4	1.180663737044597
1104	5	1.0224447606542455
1104	6	0.8536960380549843
1104	7	0.7746692257149874
1104	8	0.9348091281002127
1104	9	1.1353302859168366
1104	10	1.23489328680256
1104	11	0.89153135233515
1104	12	0.9122040959890114
1104	13	0.9452295283641519
1104	14	1.8078151191400025
1104	15	1.3761017757477987
1104	16	1.4301976333220239
1104	17	1.7895340552273282
1104	18	1.9455809217190918
1104	19	1.3435898592572286
1104	20	1.1545674639442325
1104	21	1.1253697128056501
1104	22	1.2631868678361755
1104	23	1.6966305744718397
1104	24	1.4518128218441007
1104	25	1.7267989164054995
1105	1	0.6249285165376861
1105	2	0.8430802458452291
1105	3	0.7380495697753773
1105	4	1.0764696263802165
1105	5	0.9363228691703114
1105	6	0.6578506672126991
1105	7	0.6307140401616991
1105	8	0.7448874189490553
1105	9	0.941628793515882
1105	10	1.314679212200133
1105	11	0.9787093904370892
1105	12	0.8348166884360992
1105	13	0.7431903248594978
1105	14	1.5333272856436295
1105	15	1.1568733031208822
1105	16	1.3750399454895756
1105	17	1.5579433681642811
1105	18	1.2873426322276345
1105	19	1.0281411046214117
1105	20	0.8506152019801902
1105	21	0.9079700496710075
1105	22	1.0064490061154174
1105	23	1.2908725393326463
1105	24	1.0731435378689582
1105	25	1.3656179168642666
1106	1	0.5009692887805812
1106	2	0.777562278556772
1106	3	0.6611201810323593
1106	4	1.140091674522516
1106	5	0.9790879919319195
1106	6	0.7092957190011333
1106	7	0.6408631574561738
1106	8	0.669113003513317
1106	9	0.806834723571427
1106	10	0.7296040857882389
1106	11	0.7085713743675299
1106	12	0.7680248802171548
1106	13	0.6626527787642686
1106	14	1.5523966597440193
1106	15	1.0400050411133037
1106	16	1.1878606417816329
1106	17	1.5347437006306706
1106	18	1.6722579030747085
1106	19	1.294615872019378
1106	20	1.1018386181501256
1106	21	0.9208752884536366
1106	22	1.2404981895713263
1106	23	1.7228665184347562
1106	24	1.3552755174854312
1106	25	1.5348797087398935
1107	1	0.7452039068867045
1107	2	0.9202993835189623
1107	3	0.8602360512468579
1107	4	1.173569097407352
1107	5	1.1368166839567024
1107	6	0.7805071717035545
1107	7	0.7626139432121875
1107	8	0.8297674539366398
1107	9	1.165594051852878
1107	10	1.3184364256133208
1107	11	0.8378174394166109
1107	12	0.769351871964659
1107	13	0.7769720507674904
1107	14	1.7527824412627098
1107	15	1.2117626662377532
1107	16	1.304188528303655
1107	17	1.8326385635964684
1107	18	1.9549241151547818
1107	19	1.3330227825583876
1107	20	1.1784698846571757
1107	21	1.0566498649270315
1107	22	1.3354131962769955
1107	23	1.6139945719813582
1107	24	1.4687846071603516
1107	25	1.7230919286857542
1108	1	0.5021967217993115
1108	2	0.684069691218955
1108	3	0.506616680322967
1108	4	0.9800879219265397
1108	5	0.9203986760353047
1108	6	0.4946843697115568
1108	7	0.4771740214785467
1108	8	0.5185782585870342
1108	9	0.726480848630537
1108	10	1.1377319259379348
1108	11	0.6918360814594209
1108	12	0.485655784130131
1108	13	0.47859154462284437
1108	14	1.364155393525607
1108	15	0.91870983004263
1108	16	1.0390671270899574
1108	17	1.286604191449662
1108	18	1.546335304222076
1108	19	1.0317678572968632
1108	20	0.740521456963279
1108	21	0.8230182520791587
1108	22	1.133990776909144
1108	23	1.36933984930063
1108	24	1.0734718810657142
1108	25	1.3403363945293734
1109	1	0.23005780308119128
1109	2	0.46447524482346125
1109	3	0.4391687342716857
1109	4	0.6796510275532981
1109	5	0.809788583344222
1109	6	0.4040038110040314
1109	7	0.39247635466692543
1109	8	0.3518558919025736
1109	9	0.6840209532772619
1109	10	0.8971273736659171
1109	11	0.49568382009363887
1109	12	0.3671350633431629
1109	13	0.1882908279182871
1109	14	1.1844079707079587
1109	15	0.7676397177508889
1109	16	0.6927778766440014
1109	17	1.2377944940681118
1109	18	1.3467879196929076
1109	19	0.765974429983487
1109	20	0.5495386474763606
1109	21	0.44006233074043166
1109	22	0.5319693480388707
1109	23	0.9801609089786751
1109	24	0.6203884540185811
1109	25	0.9164129410606563
1110	1	0.29012158763115536
1110	2	0.5620750953701226
1110	3	0.47282514183647706
1110	4	0.7901271431898564
1110	5	0.6512042089280214
1110	6	0.3144227449233199
1110	7	0.43257942014073336
1110	8	0.4196345131559909
1110	9	0.5805927190506708
1110	10	0.9568214786608422
1110	11	0.5692245990104396
1110	12	0.4897626645676567
1110	13	0.25080744620042594
1110	14	1.1775619798036558
1110	15	0.7601873139936046
1110	16	1.01058343310563
1110	17	1.2544345220609614
1110	18	0.9732561356529814
1110	19	0.4719501912614348
1110	20	0.3573843546960802
1110	21	0.2111036706014744
1110	22	0.56825873398531
1110	23	-0.6234309133932641
1110	24	0.15060778074710157
1110	25	0.6807881875548745
1111	1	0.10814644950960783
1111	2	0.48324034422007145
1111	3	0.3925749819468187
1111	4	0.7414046230206424
1111	5	0.5944790187941749
1111	6	0.3218667642795978
1111	7	0.27225921702307687
1111	8	0.3674305401869731
1111	9	0.6587628686149216
1111	10	0.7662797224174369
1111	11	0.47668990488128316
1111	12	0.2552228385584243
1111	13	0.2078082051947021
1111	14	1.1689823586072947
1111	15	0.8259087053033127
1111	16	0.8402865006753899
1111	17	1.274993827638987
1111	18	1.4726782212305451
1111	19	0.9743326434679105
1111	20	0.5166200050809735
1111	21	0.35152676175013653
1111	22	0.7851654277754321
1111	23	1.099279485984134
1111	24	0.7302814710003886
1111	25	0.9555590444863853
1112	1	0.17492550919978456
1112	2	0.39885562348094794
1112	3	0.25319796041997833
1112	4	0.6016668218228105
1112	5	0.3132604540202024
1112	6	0.17538344719586263
1112	7	0.01755650720986779
1112	8	0.12284320248946301
1112	9	0.346992165858687
1112	10	0.595648348320843
1112	11	0.1746138258308747
1112	12	0.18752587026834533
1112	13	0.11088034565309357
1112	14	0.6417246323123535
1112	15	0.4736233160094727
1112	16	0.3700542088636354
1112	17	0.836036200389195
1112	18	1.0612423761742917
1112	19	0.5107307662529337
1112	20	0.3636649003871639
1112	21	0.28388299153486685
1112	22	0.6902832760196986
1112	23	0.9854172283739047
1112	24	0.6215359009491621
1112	25	1.0194131772997324
1113	1	0.03839863794128462
1113	2	0.06733766136846597
1113	3	-0.011763709982158588
1113	4	0.19795654377637817
1113	5	0.30913237273944816
1113	6	0.07910434285943069
1113	7	-0.07160203697969081
1113	8	0.04528416371199029
1113	9	0.13157905368066025
1113	10	0.4802350913571303
1113	11	0.008412645151850029
1113	12	-0.15156510726670547
1113	13	-0.22652194813830562
1113	14	0.4698876687192026
1113	15	0.10013994609361987
1113	16	0.18821810433222907
1113	17	0.48393574614822654
1113	18	0.4926158904318214
1113	19	0.18491398192376352
1113	20	-0.07690998566650364
1113	21	-0.15700921648549127
1113	22	0.10475450903392414
1113	23	0.21272834579124122
1113	24	0.04145879774399219
1113	25	0.5926351607864362
1114	1	-1.1458687505330971
1114	2	-0.8634297254412289
1114	3	-0.8582176203050658
1114	4	-0.5804364542702345
1114	5	-3.788092405964374
1114	6	-1.436529433898606
1114	7	-1.1993171987942723
1114	8	-0.7532122921721722
1114	9	-0.5716129573500197
1114	10	-0.3496739835104421
1114	11	-0.8657233235216211
1114	12	-0.9051749781869773
1114	13	-0.8459560120784033
1114	14	-6.4073660184406975
1114	15	-2.6921705377639213
1114	16	-1.518271411239482
1114	17	-0.5101051547035667
1114	18	-6.857910792339489
1114	19	-2.7531682716230854
1114	20	-1.688282690243291
1114	21	-1.340533441705098
1114	22	-1.1288617733043793
1114	23	-0.5558587022882122
1114	24	-0.6987458349335967
1114	25	-0.48925405771021957
1115	1	-0.6779511032658085
1115	2	-0.4287196046763384
1115	3	-0.445885674367716
1115	4	-3.062468422138057
1115	5	-1.1243063866540481
1115	6	-0.7014267967416643
1115	7	-0.3917505634009686
1115	8	-0.39018226444956383
1115	9	-0.09645583688359238
1115	10	-0.4045247952947264
1115	11	-0.6345628791959186
1115	12	-0.6373723451031665
1115	13	-0.6526946742181465
1115	14	-6.088692276031509
1115	15	-2.484118900583738
1115	16	-1.1836190276867384
1115	17	-0.301420916502245
1115	18	-0.12149267182957146
1115	19	-0.4200751840031245
1115	20	-0.36221046142811986
1115	21	-0.63927323327583
1115	22	-1.6915993870155077
1115	23	-0.9028576214126218
1115	24	-0.6460234068686646
1115	25	-0.23767905605910045
1116	1	-0.5083060853248185
1116	2	-0.42628178997500044
1116	3	-0.46911322820641743
1116	4	-0.1877629123350033
1116	5	-0.8301318085452039
1116	6	-0.6096332009728442
1116	7	-0.6278163589177126
1116	8	-0.5059832337454111
1116	9	-0.5620982627655948
1116	10	-0.3493823054500993
1116	11	-0.6379396861399513
1116	12	-0.9473771475425536
1116	13	-0.8534608625282942
1116	14	-0.049124160034772046
1116	15	-0.4039333670411054
1116	16	-0.5066687444686409
1116	17	-0.03909196408414317
1116	18	-0.08099112099816352
1116	19	-0.37763797747096817
1116	20	-0.7832974694664401
1116	21	-0.8078589882759246
1116	22	-0.8679218928403003
1116	23	-1.5827477734758872
1116	24	-1.2708508650780033
1116	25	-0.7232801264368618
1201	1	0.7885642406175961
1201	2	1.021812519687579
1201	3	0.9730243299564343
1201	4	1.3383520052402602
1201	5	1.1598073725310982
1201	6	0.8955154354986945
1201	7	0.8173218145786905
1201	8	0.9365087749844889
1201	9	1.0809077914713328
1201	10	1.4754499758241693
1201	11	0.9522048365847553
1201	12	1.0619450406481121
1201	13	0.8477959620278952
1201	14	1.795222491697956
1201	15	1.521536948300053
1201	16	1.660703942557042
1201	17	1.9879315499544532
1201	18	1.4570759019627673
1201	19	1.3060856432715369
1201	20	1.3859746854224682
1201	21	1.135075392276768
1201	22	1.3538881096717361
1201	23	1.7318570648434886
1201	24	1.3392278146512453
1201	25	1.7956868967545319
1202	1	0.8839385997826916
1202	2	1.1021978967733936
1202	3	1.0362672996638942
1202	4	1.4283512482738772
1202	5	1.313531005414454
1202	6	0.8918370765389483
1202	7	0.9548161144122886
1202	8	0.9390972595089835
1202	9	1.2416177787914933
1202	10	1.426986709254919
1202	11	1.0218518990444352
1202	12	0.9209879470218496
1202	13	0.9732214936920087
1202	14	1.9415421290036718
1202	15	1.501851762498859
1202	16	1.5607799273139875
1202	17	2.0046670551614127
1202	18	2.1464813323714935
1202	19	1.6128547824069344
1202	20	1.3274107632784222
1202	21	1.3457844497593285
1202	22	1.6124315491960175
1202	23	1.8715929281034036
1202	24	1.5607802191196
1202	25	1.9785576642582043
1203	1	0.5524003886423507
1203	2	0.6106387657736185
1203	3	0.5215410506354772
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2211	18	-0.3210070896890542
2211	19	-0.4414027926857287
2211	20	-0.5606425437143088
2211	21	-0.4601839380465407
2211	22	-0.29755035899518134
2211	23	-0.1616160906769224
2211	24	-0.5985123680983122
2211	25	-7.7914252059203655
2212	1	-1.7152119714612937
2212	2	-4.04944542060753
2212	3	-1.9092243400274604
2212	4	-3.840992971522226
2212	5	-1.5801642880405247
2212	6	-1.3448170136183357
2212	7	-1.243457312473101
2212	8	-1.1424758103171513
2212	9	-1.1256701127337578
2212	10	-0.7011989208950098
2212	11	-1.1770303028669957
2212	12	-1.3108919187910644
2212	13	-1.3235321257357313
2212	14	-6.867536945667972
2212	15	-5.37379292783314
2212	16	-3.018966333318698
2212	17	-7.526116991398865
2212	18	-3.2932677312889425
2212	19	-2.225458443599649
2212	20	-2.085150504542163
2212	21	-1.7886115328580274
2212	22	-1.7560633628729008
2212	23	-1.2501375082515267
2212	24	-1.6785069743113397
2212	25	-1.4633195703879025
2213	1	0.05315634698944294
2213	2	0.047012613549703985
2213	3	0.035872393829706084
2213	4	0.45785475154015387
2213	5	0.35142572012664175
2213	6	-0.05110942267936025
2213	7	-0.006873719426245373
2213	8	0.08604666569292618
2213	9	0.3572258047687953
2213	10	-3.527043910400831
2213	11	-1.2165085322032496
2213	12	-0.6231120212518988
2213	13	-0.4879864738019606
2213	14	0.5525503262262887
2213	15	0.006425073075931209
2213	16	0.22603523763878286
2213	17	0.5669694827930627
2213	18	0.6450531732203828
2213	19	0.044423788916546414
2213	20	-0.26472509197341765
2213	21	-0.3046706117246174
2213	22	-0.5623746053146519
2213	23	-0.6588563901644164
2213	24	-0.2788810840266507
2213	25	0.11722957658671618
2214	1	-0.9486126734689115
2214	2	-0.6833075598838505
2214	3	-0.7030561117854468
2214	4	-0.33030576840612014
2214	5	-3.5593352045215454
2214	6	-1.4706326133038878
2214	7	-1.3073748569859092
2214	8	-0.8479434222996218
2214	9	-0.6478566683071918
2214	10	-4.456736919901758
2214	11	-1.9302437235507028
2214	12	-1.2844740629057227
2214	13	-1.0361715168414065
2214	14	-0.2543819216412331
2214	15	-0.5975546581623021
2214	16	-0.561685186695783
2214	17	-0.19194715995295297
2214	18	0.04064885049363909
2214	19	-0.6622335486976993
2214	20	-0.9897712056655976
2214	21	-0.9900963815264845
2214	22	-0.7028731913386181
2214	23	-0.5601037633229708
2214	24	-1.0760767425177704
2214	25	-0.639593566550893
2215	1	-2.3356630175885336
2215	2	-4.880838265967796
2215	3	-3.0394372304254382
2215	4	-2.181473292498435
2215	5	-1.9682316770244874
2215	6	-2.221743391646715
2215	7	-2.36538983287225
2215	8	-2.537690634434803
2215	9	-8.508963893472878
2215	10	-3.9875083534084155
2215	11	-3.190319213787351
2215	12	-3.5384848511659186
2215	13	-2.9368795908289336
2215	14	-8.173724143828426
2215	15	-4.821360485241456
2215	16	-9.670815012957899
2215	17	-4.775783750584257
2215	18	-3.155526591075642
2215	19	-3.80762844066075
2215	20	-3.442390475182293
2215	21	-3.351372399025717
2215	22	-9.282468956687058
2215	23	-10.425225224841274
2215	24	-6.574982547857516
2215	25	-5.009250418961372
2216	1	-1.798888188464712
2216	2	-1.7683698759903628
2216	3	-1.619119178356062
2216	4	-6.709438414973892
2216	5	-3.4380496726794867
2216	6	-2.572307019719382
2216	7	-2.047013029213975
2216	8	-7.441353005795175
2216	9	-3.622223334911162
2216	10	-2.001349722430639
2216	11	-1.9221152631557104
2216	12	-2.164997920704998
2216	13	-1.9155587067890778
2216	14	-1.3040291658956704
2216	15	-1.9447082550295391
2216	16	-1.5019916293391944
2216	17	-1.2634221259877165
2216	18	-7.817437572626421
2216	19	-4.830910857283897
2216	20	-3.99208435295656
2216	21	-3.2795642416630493
2216	22	-2.9064110308421185
2216	23	-2.0423760081259914
2216	24	-2.3687900307480234
2216	25	-1.8263396933849663
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	83228.0
3	3.0
4	680.0
5	4088.0
6	4992.0
7	4705.0
8	4416.0
9	4019.0
10	3743.0
11	3622.0
12	3547.0
13	3506.0
14	3526.0
15	3517.0
16	3559.0
17	3785.0
18	4332.0
19	5367.0
20	6895.0
21	8680.0
22	10764.0
23	13027.0
24	15067.0
25	17664.0
26	19805.0
27	22245.0
28	24653.0
29	29149.0
30	35510.0
31	42163.0
32	53522.0
33	69499.0
34	91259.0
35	122000.0
36	172919.0
37	250059.0
38	685993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87497796361418	17.783349716215014	11.853474271598524	25.488198048572286
2	14.871142509289548	19.210397958074545	41.03333968749539	24.885119845140512
3	19.55144679165927	23.389056547090714	28.986522101295897	28.072974559954115
4	11.5550875334004	15.504024467536617	38.41721791235604	34.52367008670694
5	12.859098995175511	37.654947082447826	36.0168706956868	13.469083226689868
6	33.24545648733832	36.44498742333027	17.20911274537584	13.10044334395557
7	29.072453359474657	31.177945381120807	21.023369713587915	18.726231545816617
8	25.850724537386434	36.491749270231495	19.695276352993808	17.962249839388264
9	26.140343994053882	15.333422699422607	19.57591431317801	38.9503189933455
10	14.056181348847172	28.716310290541138	34.821232493394064	22.40627586721763
11	35.524965655841285	22.545571061211344	22.93530437834212	18.99415890460525
12	22.867718345412545	24.687692612035832	31.06588605487498	21.378702987676647
13	29.155778724684346	21.00103488809966	25.448710368118256	24.39447601909775
14	20.6144892153764	20.89410469640658	28.19615045067629	30.295255637540734
15	23.72558028752895	29.152516137190236	23.10200317538699	24.019900399893825
16	22.809118873954752	28.195107065301794	26.33749206444353	22.65828199629992
17	21.983531073815847	27.959294238710044	27.434529804962914	22.622644882511196
18	21.968966190019955	27.4805411951156	28.462773488720376	22.087719126144066
19	23.390834163493167	26.946116268880232	27.43865354977798	22.22439601784862
20	23.52627336771379	26.86885850731107	27.505167696027755	22.09970042894738
21	23.465175101411504	26.506511972906793	27.065140439196426	22.963172486485277
22	23.13692466595265	26.931560831636865	27.207737525855798	22.723776976554685
23	22.62276445715077	26.722969530076178	27.629148802941977	23.025117209831077
24	22.919309131759782	26.744572033757418	27.53552644990595	22.800592384576852
25	22.999061485251364	26.598990415377255	27.496629086109	22.90531901326238
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	143.0
1	143.0
2	272.0
3	401.0
4	401.0
5	401.0
6	930.0
7	1459.0
8	1459.0
9	1459.0
10	2403.5
11	3348.0
12	3348.0
13	3348.0
14	5681.5
15	8015.0
16	8015.0
17	8015.0
18	13853.5
19	19692.0
20	19692.0
21	19692.0
22	30126.0
23	40560.0
24	40560.0
25	40560.0
26	57021.5
27	73483.0
28	73483.0
29	73483.0
30	94406.0
31	115329.0
32	115329.0
33	115329.0
34	135455.5
35	155582.0
36	155582.0
37	155582.0
38	174976.5
39	194371.0
40	194371.0
41	194371.0
42	208555.5
43	222740.0
44	222740.0
45	222740.0
46	233855.5
47	244971.0
48	244971.0
49	244971.0
50	240546.5
51	236122.0
52	236122.0
53	236122.0
54	214802.5
55	193483.0
56	193483.0
57	193483.0
58	168235.5
59	142988.0
60	142988.0
61	142988.0
62	117500.5
63	92013.0
64	92013.0
65	92013.0
66	71386.0
67	50759.0
68	50759.0
69	50759.0
70	37622.5
71	24486.0
72	24486.0
73	24486.0
74	17179.5
75	9873.0
76	9873.0
77	9873.0
78	6880.0
79	3887.0
80	3887.0
81	3887.0
82	2593.5
83	1300.0
84	1300.0
85	1300.0
86	845.0
87	390.0
88	390.0
89	390.0
90	238.5
91	87.0
92	87.0
93	87.0
94	53.0
95	19.0
96	19.0
97	19.0
98	13.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18038602937170525
2	0.2553516519677386
3	0.25785777016498973
4	0.4594913233829545
5	0.2528455337704875
6	0.019831022256508825
7	0.019340694783133605
8	0.018360039836383173
9	0.019613098935008726
10	0.02260954460563506
11	0.022173697962634867
12	0.02925620591138802
13	0.02871139760763778
14	0.026586645223011834
15	0.04200472021914369
16	0.022500582944885012
17	0.04249504769251891
18	0.033015383207264694
19	0.02833003179501261
20	0.030018937536638354
21	0.03579390555639093
22	0.044292915094894705
23	0.030018937536638354
24	0.04843345820339655
25	0.03780969628026682
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1835508.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.96801239882787
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.91350825296377	58.95152377580784
2	11.980288196721217	17.243950589663275
3	3.201430194165975	6.912017037231564
4	1.2096854084637279	3.482346178999949
5	0.5542265204629531	1.9943290548218515
6	0.310447779977948	1.3405385807184926
7	0.1917942516894234	0.9662135758525835
8	0.12826147201938953	0.7384578566866673
9	0.0859657134503464	0.5568103378321752
>10	0.3985001689006288	4.863147411763006
>50	0.016000820552397852	0.7634713849363667
>100	0.008358317523122546	1.1136152333215443
>500	0.001149677331878929	0.5614184289424976
>1k	3.832257772929751E-4	0.5121605534221926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2954	0.16093637292782162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	5.448083037502424E-5	0.0	0.0	0.0
6	0.0	5.448083037502424E-5	0.0	0.0	0.0
7	0.0	5.448083037502424E-5	0.0	5.448083037502424E-5	0.0
8	0.0	5.448083037502424E-5	0.0	5.448083037502424E-5	0.0
9	5.448083037502424E-5	5.448083037502424E-5	0.0	5.448083037502424E-5	0.0
10	5.448083037502424E-5	5.448083037502424E-5	0.0	5.448083037502424E-5	0.0
11	5.448083037502424E-5	5.448083037502424E-5	0.0	1.0896166075004848E-4	0.0
12	5.448083037502424E-5	5.448083037502424E-5	0.0	1.0896166075004848E-4	0.0
13	5.448083037502424E-5	5.448083037502424E-5	0.0	1.0896166075004848E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAGGAC	735	0.0	13.090965	3
TAGGACC	465	0.0	12.497252	4
TGTAGGA	900	0.0	11.651584	2
CTGTAGG	900	0.0	11.353707	1
CTAGACA	220	0.0	11.258701	4
GGACCGT	85	5.391815E-5	11.162557	6
GTCCTAC	550	0.0	10.938917	1
TTAGACT	185	5.456968E-12	10.8139715	4
TAGGACT	265	0.0	10.784823	4
GTCCTAA	355	0.0	10.760391	1
TAGGACG	435	0.0	10.731105	4
AGGCGTG	125	2.2773747E-7	10.627335	7
GGACGTG	460	0.0	10.519497	6
TATGGCG	555	0.0	10.430432	16
GTGCGCC	110	6.1225546E-6	10.35271	11
TAAGCTG	195	1.4551915E-11	10.240077	5
ATATGGC	1085	0.0	10.2334385	15
TCCTACA	645	0.0	10.1982155	2
GACTAGA	160	5.662514E-9	10.146713	1
TGGCGAG	535	0.0	10.111099	18
>>END_MODULE