FastQCFastQC Report
Thu 26 May 2016
SRR1513180_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513180_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1835508
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT46190.251646955502237No Hit
TATCAACGCAGAGTACTTTTTTTTT30710.16731063008169944No Hit
GGTATCAACGCAGAGTACTTTTTTT29770.16218943202644717No Hit
GTACATGGGAAGCAGTGGTATCAAC19640.1070003508565476No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAA456.828345E-414.7590910
GGACGTG4100.012.539076
TAGGACG4550.011.9273134
ATACTGC2250.011.4244856
AGGACGT4700.011.3440825
GTCCTAC7000.011.293421
CCTACAC3950.011.0882663
ACCGTGC951.3780022E-510.98728758
GACGTGG2950.010.9385847
GTAGGAC6450.010.923833
TATACCG700.00146449110.8810585
CGTGCGC1053.5196717E-610.84341310
TGTAGGA7150.010.7874172
CCGTGCC1158.9596324E-710.7258429
GCGAATC909.60992E-510.54536715
TATGCGA909.60992E-510.54536712
TCCTACA8700.010.3978352
CTACACG1303.7272002E-710.25330454
TAGGACC3650.09.9121964
TGTGGCG1151.0420134E-59.90050810
Produced by FastQC (version 0.11.5)