FastQCFastQC Report
Thu 26 May 2016
SRR1513179_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513179_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015650
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC21710.10770719122863592No Hit
GTATCAACGCAGAGTACTTTTTTTT21400.10616922580805199No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAT350.0021901616.2637428
CCCTTAT1251.6983904E-811.4588351
GTCCTAT3150.011.216321
GGCGAGG3400.010.88586919
TAGGACC4350.010.7319844
ATAGGAC2400.010.7185633
GCTATAC1451.3800673E-810.51322653
TGGCGAG8050.010.49232118
GGACGTG7600.010.4847936
TAAGACC1658.8402885E-1010.3934994
GTAGGAC8350.010.2692813
GTCCTAA6700.010.2616421
TAGGACA4950.010.2010264
AGGACGT7750.010.1829565
TAGGACT3200.010.1228354
TTAGGAC7400.010.0426173
TAGGACG8050.09.9416084
AATCCCG1151.0392327E-59.90286419
TGTTAGG1553.8451617E-89.8415762
AGCACCG1554.010144E-89.8148985
Produced by FastQC (version 0.11.5)