##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1513179_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2015650
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.69154863195495	31.0	30.0	34.0	25.0	34.0
2	29.56590429886141	31.0	30.0	34.0	24.0	34.0
3	29.741127179817926	31.0	30.0	34.0	25.0	34.0
4	32.83397117555131	37.0	35.0	37.0	25.0	37.0
5	32.987789050678444	37.0	35.0	37.0	26.0	37.0
6	33.378115248182965	37.0	35.0	37.0	28.0	37.0
7	33.357811127923995	37.0	35.0	37.0	28.0	37.0
8	33.241784039887875	37.0	35.0	37.0	27.0	37.0
9	34.52315134075857	39.0	35.0	39.0	27.0	39.0
10	34.10483466871729	39.0	34.0	39.0	23.0	39.0
11	34.563746186093816	39.0	35.0	39.0	27.0	39.0
12	34.558478902587254	39.0	35.0	39.0	27.0	39.0
13	34.59706595887183	39.0	35.0	39.0	27.0	39.0
14	34.85613524173343	40.0	34.0	41.0	18.0	41.0
15	35.287377272840025	40.0	35.0	41.0	25.0	41.0
16	35.134045593232955	40.0	35.0	41.0	24.0	41.0
17	34.74130230942872	39.0	34.0	41.0	18.0	41.0
18	34.478352392528464	39.0	34.0	41.0	17.0	41.0
19	35.1221070126262	40.0	34.0	41.0	25.0	41.0
20	35.33359412596433	40.0	36.0	41.0	25.0	41.0
21	35.38383846401905	40.0	36.0	41.0	26.0	41.0
22	35.07321409967008	40.0	36.0	41.0	23.0	41.0
23	34.618193138689755	40.0	34.0	41.0	18.0	41.0
24	34.87179718701164	40.0	35.0	41.0	22.0	41.0
25	34.51203185076774	40.0	34.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	0.9007724369815122
1101	2	1.1756606815187993
1101	3	1.0482978051070937
1101	4	1.388950553688609
1101	5	1.2760592624604286
1101	6	1.0121117647270808
1101	7	0.9241620072123311
1101	8	0.997182050446419
1101	9	1.3418259452888037
1101	10	1.6648387749107556
1101	11	1.208434738238502
1101	12	1.1200803891865831
1101	13	1.0334652968370364
1101	14	2.037214089646902
1101	15	1.6253476351600042
1101	16	1.679167568946987
1101	17	2.11681231001878
1101	18	2.1512072597903114
1101	19	1.7127080241054031
1101	20	1.3433502427817672
1101	21	1.2680829432704144
1101	22	1.6182286090385887
1101	23	1.951976592604872
1101	24	1.5886698672983783
1101	25	1.8297969695069085
1102	1	0.933153201349807
1102	2	1.0643172608317464
1102	3	0.9923623785282203
1102	4	1.3559875198252556
1102	5	1.3859729323667977
1102	6	0.9247696787399704
1102	7	0.977181101131869
1102	8	1.0326955044610884
1102	9	1.3446910967253984
1102	10	1.5748488915108823
1102	11	1.2449478878246012
1102	12	1.06637001641446
1102	13	1.1301622468349777
1102	14	2.034342623643859
1102	15	1.5083596567651298
1102	16	1.6732687668534751
1102	17	1.9546083800984988
1102	18	2.2967922478723963
1102	19	1.6062042200776432
1102	20	1.422730325176886
1102	21	1.2627318467293378
1102	22	1.4966472820756564
1102	23	1.797740980843031
1102	24	1.5144846790113036
1102	25	1.826821811675984
1103	1	0.8272004080018043
1103	2	1.076354264266179
1103	3	0.8748801032920817
1103	4	1.3488439478633154
1103	5	1.1379749206635594
1103	6	0.7781448894741345
1103	7	0.8382640410397784
1103	8	0.8317969218923338
1103	9	1.2476189956741734
1103	10	1.4216805384210502
1103	11	1.0719577116381913
1103	12	1.0056265605831314
1103	13	0.9535365008112962
1103	14	1.888338187366685
1103	15	1.4695512491730938
1103	16	1.5789858898496405
1103	17	1.9370740880278277
1103	18	2.2761933614505523
1103	19	1.4372378154746457
1103	20	1.1139609247121456
1103	21	1.309685232827313
1103	22	1.5952391984616199
1103	23	2.00137951992879
1103	24	1.6223102983470454
1103	25	1.8329004131819389
1104	1	0.6906359828178843
1104	2	0.95520055404765
1104	3	0.7832308816577047
1104	4	1.214671440509214
1104	5	1.0357309004581339
1104	6	0.8209036151621021
1104	7	0.7906246937467927
1104	8	0.9357444834846262
1104	9	1.1890999293866784
1104	10	1.3242388410783121
1104	11	0.9543606130415867
1104	12	0.9579779696886206
1104	13	0.8834096459738987
1104	14	1.8780293967016348
1104	15	1.4787703823407625
1104	16	1.5860469139319449
1104	17	1.9885158179950366
1104	18	1.964939548464919
1104	19	1.5168431680636658
1104	20	1.1240087195694386
1104	21	1.1679182912088137
1104	22	1.4439796218185705
1104	23	1.7730908368157614
1104	24	1.4434275779007422
1104	25	1.681721853987547
1105	1	0.47120474281108926
1105	2	0.8471167418942933
1105	3	0.6745161325629923
1105	4	0.8926665709702561
1105	5	0.7801210891190706
1105	6	0.6411922822943339
1105	7	0.5828113216584683
1105	8	0.5970467481168384
1105	9	0.8018610334723988
1105	10	1.2242277862002808
1105	11	0.5929426766367953
1105	12	0.6116367298705683
1105	13	0.5276464144982356
1105	14	1.361723990866274
1105	15	1.0389388478967305
1105	16	1.1474961275526354
1105	17	1.4477009386684685
1105	18	1.1971378244432103
1105	19	0.9647857474093797
1105	20	0.8128618973076129
1105	21	0.7064456196762023
1105	22	0.9966124353928834
1105	23	1.2477236665679428
1105	24	1.20285455773503
1105	25	1.3601106459590113
1106	1	0.6065115965658379
1106	2	0.7892431072391375
1106	3	0.7436236803483744
1106	4	1.2274373979560238
1106	5	1.114290638592344
1106	6	0.7953717002684826
1106	7	0.8747490799988356
1106	8	0.7858263165451831
1106	9	1.0879542665388868
1106	10	0.9239115088360919
1106	11	0.9654899092772666
1106	12	0.9380107029128482
1106	13	0.8110692204419863
1106	14	1.6966873345085034
1106	15	1.2961189911787372
1106	16	1.490793231444222
1106	17	1.756764590499131
1106	18	1.9371163078757192
1106	19	1.34859439555958
1106	20	1.2055144478836795
1106	21	1.0157087985737903
1106	22	1.251835595631988
1106	23	1.65525122961445
1106	24	1.2401542554784868
1106	25	1.4548806101250236
1107	1	0.5902200807794209
1107	2	0.7285527432778558
1107	3	0.7485625953892594
1107	4	1.0197846751136836
1107	5	0.903045616726672
1107	6	0.582206865402064
1107	7	0.5758891027888495
1107	8	0.7717346321837084
1107	9	0.9803624354314593
1107	10	1.3174129089282118
1107	11	0.8896409500245923
1107	12	0.8134742582172692
1107	13	0.7358278207467563
1107	14	1.579927380589865
1107	15	1.0896065000174389
1107	16	1.1638249818361501
1107	17	1.6258080889225255
1107	18	1.6897637405527561
1107	19	1.1986084434812696
1107	20	1.0575779256895288
1107	21	0.8567663302965016
1107	22	1.1123266775517422
1107	23	1.536454901500953
1107	24	1.2209960236574915
1107	25	1.529075256874627
1108	1	0.5827666538489815
1108	2	0.8856390483193337
1108	3	0.6892540567804524
1108	4	1.1205903930460437
1108	5	0.9572397711619018
1108	6	0.7066583778462245
1108	7	0.6077439081494092
1108	8	0.6403258255468245
1108	9	0.8190023843784928
1108	10	1.0915564188197209
1108	11	0.6113724624687578
1108	12	0.5375011120781465
1108	13	0.5848800224059758
1108	14	1.4732147322171798
1108	15	1.043765734222923
1108	16	1.202528746992499
1108	17	1.4129337558019088
1108	18	1.7351711687595213
1108	19	1.140116752351723
1108	20	0.8786189978018442
1108	21	0.7858361962824603
1108	22	1.0009901619167678
1108	23	1.330918921922141
1108	24	1.0719033880480424
1108	25	1.4295026723181508
1109	1	0.29317527475982175
1109	2	0.49415445563171545
1109	3	0.4407646148304849
1109	4	0.6593786419235244
1109	5	0.6948533183075085
1109	6	0.3890836247994045
1109	7	0.3896137221327294
1109	8	0.4518557806462411
1109	9	0.5488179071494983
1109	10	0.9198708768056107
1109	11	0.49157864591968803
1109	12	0.5021758009812913
1109	13	0.35444410194414644
1109	14	1.264353564802171
1109	15	0.7957201665513409
1109	16	0.9640953922779403
1109	17	1.281901145154059
1109	18	1.46794674284547
1109	19	0.7835067202798811
1109	20	0.6209751748384065
1109	21	0.6509183258527003
1109	22	0.8883645301015051
1109	23	1.1455063241030317
1109	24	0.9329683784264944
1109	25	1.2909372329339348
1110	1	0.1771471666694957
1110	2	0.4521113560116419
1110	3	0.3864632875496845
1110	4	0.5705770597127113
1110	5	0.5395838737260021
1110	6	0.1978676086154607
1110	7	0.1827141645596697
1110	8	0.211330953751947
1110	9	0.33657674335285037
1110	10	0.6054035983068999
1110	11	0.097609385545681
1110	12	0.23962008643712096
1110	13	0.029774894200848223
1110	14	0.8709644758069217
1110	15	0.5401929137101078
1110	16	0.6774749008386536
1110	17	0.8921096532378101
1110	18	0.5907101431184927
1110	19	0.3816208549158233
1110	20	0.1985518183146695
1110	21	0.01851158089784377
1110	22	0.3325911875577958
1110	23	-0.8995564626932406
1110	24	-0.11275763759298485
1110	25	0.41058164667712305
1111	1	0.34876061611313247
1111	2	0.7553467212753091
1111	3	0.697989654264731
1111	4	1.0436902672598762
1111	5	0.910368827765673
1111	6	0.5971298243871104
1111	7	0.5769328389670179
1111	8	0.6227484670562546
1111	9	0.7761872900388695
1111	10	1.0757591861153202
1111	11	0.6674569907706456
1111	12	0.5429340680529933
1111	13	0.3173353683179272
1111	14	1.291211210204601
1111	15	0.8914447279336173
1111	16	1.0125438413321248
1111	17	1.3021423092610291
1111	18	1.6370272693884473
1111	19	1.1292926014083235
1111	20	0.6518597525188312
1111	21	0.40834588181705556
1111	22	0.7750319229230556
1111	23	1.083093513117113
1111	24	0.8796286710669108
1111	25	1.029618898733247
1112	1	0.10372665384898028
1112	2	0.38035904831933465
1112	3	0.2662140567804556
1112	4	0.8012303930460405
1112	5	0.5210797711619009
1112	6	0.2250583778462243
1112	7	0.11174390814940693
1112	8	0.2716858255468182
1112	9	0.46796238437849524
1112	10	0.6566764188197212
1112	11	0.23697246246875636
1112	12	0.17654111207814793
1112	13	0.05304002240598038
1112	14	0.6341747322171827
1112	15	0.4885657342229237
1112	16	0.5462087469924981
1112	17	0.8935737558019099
1112	18	1.0945311687595165
1112	19	0.5058767523517176
1112	20	0.4101389978018446
1112	21	0.29015619628246014
1112	22	0.6560301619167674
1112	23	1.0560389219221449
1112	24	0.8053433880480441
1112	25	1.072702672318151
1113	1	0.2301475256275367
1113	2	0.29333330221688314
1113	3	0.24628425722588787
1113	4	0.6020252514615692
1113	5	0.6289900225141096
1113	6	0.2806125362935674
1113	7	0.33899878565497943
1113	8	0.42354898813034225
1113	9	0.47296509516436913
1113	10	0.6819695527681802
1113	11	0.3756699107665611
1113	12	0.2657224547443917
1113	13	0.1213549826350615
1113	14	0.97142500265943
1113	15	0.5208035706849046
1113	16	0.7234975029581392
1113	17	1.0366360529702234
1113	18	0.7846099979036509
1113	19	0.6687918780278252
1113	20	0.5444524562810074
1113	21	0.4977643159133862
1113	22	0.5777821695527834
1113	23	0.8127195964369349
1113	24	0.6253595254963429
1113	25	0.9732891692955619
1114	1	-0.7977670665675554
1114	2	-0.4769559911252834
1114	3	-0.4954025036487053
1114	4	-0.07304606009375192
1114	5	-3.2738235548084376
1114	6	-1.195531789398963
1114	7	-0.8034846686887107
1114	8	-0.4293860078391063
1114	9	-0.08321333035801359
1114	10	-0.031065278876710067
1114	11	-0.35275532547696997
1114	12	-0.45788499079341705
1114	13	-0.42882575228635034
1114	14	-5.667655245693833
1114	15	-2.082237623303108
1114	16	-1.0401729254593803
1114	17	-0.0601029876502821
1114	18	-6.541028919596428
1114	19	-2.37049752344506
1114	20	-1.236709345524126
1114	21	-0.875361455973767
1114	22	-0.7198169570482023
1114	23	-0.17784341319934782
1114	24	-0.29500948036470476
1114	25	-0.2608426479721615
1115	1	-0.7455463960133635
1115	2	-0.2932950844429776
1115	3	-0.3434876501635671
1115	4	-2.8934205950237057
1115	5	-0.9355444686667909
1115	6	-0.6119395542430937
1115	7	-0.5070702123767461
1115	8	-0.32897267552384335
1115	9	-0.0631516688487892
1115	10	-0.30478868977618134
1115	11	-0.3783812726184266
1115	12	-0.5211108671203846
1115	13	-0.497675376798675
1115	14	-5.917118996751924
1115	15	-2.4402846298027683
1115	16	-1.1868140489695662
1115	17	-0.2012080123229012
1115	18	0.0787288071810579
1115	19	-0.22880115526528755
1115	20	-0.3374359180745685
1115	21	-0.4904805244272339
1115	22	-1.6487931846723995
1115	23	-0.8797277100754073
1115	24	-0.5677108303673748
1115	25	-0.11348671893299667
1116	1	-0.28054734386006785
1116	2	0.04208514224831461
1116	3	-0.01901957437647539
1116	4	0.08022768972416117
1116	5	-0.42095560111759767
1116	6	-0.3569279680873336
1116	7	-0.32386791315643393
1116	8	-0.20730945509464505
1116	9	-0.3446484747280323
1116	10	-0.08522332917569742
1116	11	-0.429505338218533
1116	12	-0.6786678684487697
1116	13	-0.6939529672847442
1116	14	0.23401848937640324
1116	15	-0.2027965567278187
1116	16	-0.1509846550693581
1116	17	0.23495153357968945
1116	18	0.10485345971026305
1116	19	-0.17161621442949127
1116	20	-0.6249249197239237
1116	21	-0.674508729261639
1116	22	-0.6040177647728058
1116	23	-1.3245487527628512
1116	24	-0.8111286394433677
1116	25	-0.3627463998467988
1201	1	0.6084685680404007
1201	2	0.8508134377582799
1201	3	0.7174927366484418
1201	4	1.1988773897457108
1201	5	1.0751798371685055
1201	6	0.7825274207505188
1201	7	0.7345547332319171
1201	8	0.8057330862728946
1201	9	0.9601931434544042
1201	10	1.3987712042982707
1201	11	0.8127337826007661
1201	12	0.7872083067976234
1201	13	0.6873117055742952
1201	14	1.6288598807320298
1201	15	1.2966380774572457
1201	16	1.399825116629465
1201	17	1.677140752501579
1201	18	1.0415574393865796
1201	19	1.0884214388203688
1201	20	0.9590208459866645
1201	21	0.9521717738402202
1201	22	1.2736993368342624
1201	23	1.4587800440343557
1201	24	1.2337167213813771
1201	25	1.5147705271036287
1202	1	0.7464788206785684
1202	2	0.8966551187005649
1202	3	0.8178559987811056
1202	4	1.1963590733979501
1202	5	1.035451670803475
1202	6	0.8570055137211057
1202	7	0.7732573131673277
1202	8	0.9090789047257104
1202	9	1.0524720487642867
1202	10	1.4286290287903967
1202	11	0.9876719215759593
1202	12	0.8092005060175396
1202	13	0.856957702432048
1202	14	1.7248168957883792
1202	15	1.3314005859661648
1202	16	1.4562004315803136
1202	17	1.8158410741466469
1202	18	2.014530543148574
1202	19	1.5287512782559247
1202	20	1.2837452539113272
1202	21	1.0528966850410129
1202	22	1.2279912176352425
1202	23	1.547624089729247
1202	24	1.1981350987029842
1202	25	1.6822311441330768
1203	1	0.5443887047960736
1203	2	0.6485911580515342
1203	3	0.5638096674924071
1203	4	0.8849521957893458
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2211	13	-0.5966085851889602
2211	14	-0.06249868550433746
2211	15	-0.3571740126125178
2211	16	-6.751529480855602
2211	17	-1.9880794087550484
2211	18	-0.8281108565569397
2211	19	-0.7881900830913153
2211	20	-1.0499673313120752
2211	21	-0.9074012720719651
2211	22	-0.8496954077034857
2211	23	-0.5075043692170951
2211	24	-0.9613254727114509
2211	25	-7.69289125173248
2212	1	-1.6339644070851342
2212	2	-4.185100747535909
2212	3	-1.9131352039680785
2212	4	-3.8299513452774967
2212	5	-1.505529718476204
2212	6	-1.2653096023579167
2212	7	-1.103358943690985
2212	8	-0.9105487800826282
2212	9	-0.9618733347678088
2212	10	-0.4565379579201476
2212	11	-0.8881260590283091
2212	12	-1.0267432182651888
2212	13	-1.2314377010706679
2212	14	-6.586714349131412
2212	15	-5.051508153806768
2212	16	-2.8799244543684637
2212	17	-7.8815982825525595
2212	18	-3.4261905881226333
2212	19	-2.042142598096781
2212	20	-2.0550997278399734
2212	21	-1.6167185578159007
2212	22	-1.5631472708082512
2212	23	-0.9444299429092808
2212	24	-1.424516086124548
2212	25	-1.2222988247433975
2213	1	-0.35487156098635353
2213	2	-0.472428443216522
2213	3	-0.38149430887051494
2213	4	-0.022520816556912848
2213	5	-0.2233872771606613
2213	6	-0.4990065775246535
2213	7	-0.6039207763689021
2213	8	-0.5189517367800462
2213	9	-0.32825326351316164
2213	10	-3.9268613589580568
2213	11	-1.7928344196488197
2213	12	-1.28218425572733
2213	13	-1.0684242558334773
2213	14	-0.2371835934214772
2213	15	-0.8047874944628219
2213	16	-0.5212119732291072
2213	17	-0.13754769695277957
2213	18	0.023018604893714212
2213	19	-0.6478245957553881
2213	20	-1.0076604481815323
2213	21	-1.034567177063181
2213	22	-1.396904870400867
2213	23	-1.6481320599184244
2213	24	-1.0495088864979678
2213	25	-0.6693482725879747
2214	1	-0.7496620759713046
2214	2	-0.451137607178687
2214	3	-0.5454253462046204
2214	4	-0.01882034103864072
2214	5	-3.417195123751288
2214	6	-1.1730554448768942
2214	7	-0.897911748262409
2214	8	-0.6028473697014292
2214	9	-0.4383510119053895
2214	10	-3.9711975013752223
2214	11	-1.489897936556524
2214	12	-1.0084367252657458
2214	13	-0.7184630174965534
2214	14	-0.0648732117365185
2214	15	-0.25978238639845586
2214	16	-0.2631706742685438
2214	17	0.16970802324023282
2214	18	0.4422992345529977
2214	19	-0.398772215056141
2214	20	-0.6859916144430471
2214	21	-0.7164274406349875
2214	22	-0.3736087780771413
2214	23	-0.2920999266919324
2214	24	-0.6422300752477241
2214	25	-0.314919575625801
2215	1	-2.0131256622273135
2215	2	-4.699650736724564
2215	3	-2.739333191175948
2215	4	-1.8141716535782386
2215	5	-1.7820651516355568
2215	6	-1.8338780072582246
2215	7	-2.0016101094103824
2215	8	-2.144561125709611
2215	9	-7.733289229306035
2215	10	-3.617445676245371
2215	11	-2.8523597567259174
2215	12	-3.110018936362664
2215	13	-2.5059959085658647
2215	14	-7.942909857549697
2215	15	-4.68253147437532
2215	16	-9.2998082799527
2215	17	-4.319014557852341
2215	18	-2.871739203629218
2215	19	-3.402766590064264
2215	20	-3.1590371450985444
2215	21	-3.324584730148054
2215	22	-9.301216656127433
2215	23	-10.414475592155966
2215	24	-6.555081340986167
2215	25	-4.999744630134163
2216	1	-1.6285518748079895
2216	2	-1.6498246072267158
2216	3	-1.47161560960107
2216	4	-6.78953842360637
2216	5	-3.3100539385615093
2216	6	-2.499147701423805
2216	7	-1.8801118317992653
2216	8	-7.975742152782235
2216	9	-3.7327412141387555
2216	10	-1.8955905672082238
2216	11	-1.9132705030288193
2216	12	-2.1199541716401313
2216	13	-1.7738028404397568
2216	14	-1.1632868018575309
2216	15	-1.7590725663188493
2216	16	-1.437039202822156
2216	17	-1.058580450072057
2216	18	-7.157590234148948
2216	19	-4.304722118478047
2216	20	-3.6545850398371584
2216	21	-2.910589831661646
2216	22	-2.5796979703900433
2216	23	-1.9193358599426986
2216	24	-2.1613788018578575
2216	25	-1.5338372079213656
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79137.0
3	2.0
4	676.0
5	4089.0
6	4777.0
7	4505.0
8	4214.0
9	3883.0
10	3833.0
11	3617.0
12	3508.0
13	3521.0
14	3546.0
15	3751.0
16	3759.0
17	3976.0
18	4559.0
19	5495.0
20	6982.0
21	9103.0
22	11099.0
23	13460.0
24	15705.0
25	18068.0
26	20374.0
27	23182.0
28	25826.0
29	30622.0
30	37003.0
31	44763.0
32	57150.0
33	75226.0
34	99251.0
35	133559.0
36	192082.0
37	281848.0
38	779499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.477820111005045	18.57557938739011	12.36743673164737	25.579163769957475
2	14.638578935196122	20.272505042014714	40.918330552246715	24.17058547054245
3	18.467393045189258	24.856380581759364	29.696815055990434	26.979411317060944
4	11.524515017198537	16.8023877609807	39.034699082910805	32.638398138909956
5	12.899072019226082	37.78927903185215	36.2109457764391	13.10070317248267
6	31.93386768077892	37.032580374168916	18.23293188633117	12.800620058720991
7	27.959100533274977	31.49751176385649	22.56859210344155	17.97479559942698
8	25.315061315263765	35.89252734798707	20.960025246585907	17.83238609016326
9	26.651866414658798	14.916480629748408	19.960988070646227	38.470664884946565
10	13.901745161468002	28.56712562195165	35.6426986709699	21.888430545610447
11	34.502585040359776	22.36869984800886	24.35654353382634	18.772171577805025
12	23.073808117751565	24.70013746284285	31.599531534571657	20.62652288483393
13	28.813269986015815	21.606404338371544	25.702260991080493	23.878064684532145
14	20.70611132118354	21.1451792347872	28.673744949577657	29.474964494451605
15	23.53301734620443	29.567809018041046	23.418765664937837	23.480407970816685
16	22.693579536770734	27.59472302113293	27.10237569247198	22.609321749624364
17	21.838737599437767	27.71794912518587	28.081454099307734	22.361859176068627
18	21.81736613273106	27.18049345992158	29.09088021708783	21.911260190259537
19	23.473037726935278	26.934383796037142	27.39167847742458	22.200899999603
20	23.86259534408234	26.81792692065288	27.38366409107376	21.935813644191022
21	23.581463490166573	26.50801701668119	26.844300940364196	23.06621855278804
22	23.418955117429054	27.15854895123117	26.84268492364717	22.57981100769261
23	22.596512680440203	26.94132021089073	27.442691874037255	23.019475234631813
24	23.19351247358784	26.793408636633696	27.346444651478606	22.666634238299853
25	23.0098237053345	26.67855132853778	27.396345863512334	22.915279102615386
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	220.0
1	220.0
2	319.0
3	418.0
4	418.0
5	418.0
6	857.0
7	1296.0
8	1296.0
9	1296.0
10	2060.0
11	2824.0
12	2824.0
13	2824.0
14	5514.5
15	8205.0
16	8205.0
17	8205.0
18	15195.5
19	22186.0
20	22186.0
21	22186.0
22	34945.0
23	47704.0
24	47704.0
25	47704.0
26	68017.0
27	88330.0
28	88330.0
29	88330.0
30	113340.0
31	138350.0
32	138350.0
33	138350.0
34	160777.5
35	183205.0
36	183205.0
37	183205.0
38	202441.0
39	221677.0
40	221677.0
41	221677.0
42	236391.5
43	251106.0
44	251106.0
45	251106.0
46	260269.5
47	269433.0
48	269433.0
49	269433.0
50	261245.0
51	253057.0
52	253057.0
53	253057.0
54	227292.0
55	201527.0
56	201527.0
57	201527.0
58	173671.5
59	145816.0
60	145816.0
61	145816.0
62	118873.5
63	91931.0
64	91931.0
65	91931.0
66	70966.5
67	50002.0
68	50002.0
69	50002.0
70	36654.0
71	23306.0
72	23306.0
73	23306.0
74	16384.5
75	9463.0
76	9463.0
77	9463.0
78	6573.0
79	3683.0
80	3683.0
81	3683.0
82	2507.0
83	1331.0
84	1331.0
85	1331.0
86	891.0
87	451.0
88	451.0
89	451.0
90	274.0
91	97.0
92	97.0
93	97.0
94	60.0
95	23.0
96	23.0
97	23.0
98	16.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1641654057003944
2	0.25029146925309453
3	0.2544092476372386
4	0.4506734800188525
5	0.25044030461637684
6	0.01771140823059559
7	0.01820752610820331
8	0.016272666385533204
9	0.018306749683724855
10	0.020886562647284994
11	0.019894326892069557
12	0.027782601146032298
13	0.02559968248455833
14	0.023416763823084365
15	0.03968943020861757
16	0.020142385830873414
17	0.04078088953935455
18	0.030412025897353215
19	0.026691141815295312
20	0.028774836901247738
21	0.03403368640388957
22	0.042616525686503114
23	0.02867561332572619
24	0.04728003373601568
25	0.03611738148984198
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2015650.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.83636353476696
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9052093483851	62.79160749256474
2	11.150010599280204	16.68852493248476
3	2.6662154053103904	5.9858959600141315
4	0.9488368539914342	2.840299989619503
5	0.4494770205243427	1.681861285424181
6	0.2318021534920696	1.040833813612462
7	0.14832407001995693	0.7770023817478808
8	0.10008628375254704	0.5992074812599559
9	0.07247515264085222	0.4881399183241688
>10	0.30115428884874085	3.8096623969686307
>50	0.016027847891867817	0.8102240256542282
>100	0.008573883088668965	1.2174729683366936
>500	0.0011377991538377738	0.5059338440724902
>1k	6.692936199044191E-4	0.7633335099161688
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	2171	0.10770719122863592	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2140	0.10616922580805199	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	4.961178776077196E-5	0.0	0.0	0.0
6	0.0	4.961178776077196E-5	0.0	0.0	0.0
7	0.0	4.961178776077196E-5	0.0	0.0	0.0
8	0.0	4.961178776077196E-5	0.0	0.0	0.0
9	0.0	4.961178776077196E-5	0.0	0.0	0.0
10	0.0	4.961178776077196E-5	0.0	0.0	0.0
11	0.0	4.961178776077196E-5	0.0	0.0	0.0
12	0.0	4.961178776077196E-5	0.0	0.0	0.0
13	0.0	9.922357552154392E-5	0.0	4.961178776077196E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGTAT	35	0.00219016	16.263742	8
CCCTTAT	125	1.6983904E-8	11.458835	1
GTCCTAT	315	0.0	11.21632	1
GGCGAGG	340	0.0	10.885869	19
TAGGACC	435	0.0	10.731984	4
ATAGGAC	240	0.0	10.718563	3
GCTATAC	145	1.3800673E-8	10.5132265	3
TGGCGAG	805	0.0	10.492321	18
GGACGTG	760	0.0	10.484793	6
TAAGACC	165	8.8402885E-10	10.393499	4
GTAGGAC	835	0.0	10.269281	3
GTCCTAA	670	0.0	10.261642	1
TAGGACA	495	0.0	10.201026	4
AGGACGT	775	0.0	10.182956	5
TAGGACT	320	0.0	10.122835	4
TTAGGAC	740	0.0	10.042617	3
TAGGACG	805	0.0	9.941608	4
AATCCCG	115	1.0392327E-5	9.902864	19
TGTTAGG	155	3.8451617E-8	9.841576	2
AGCACCG	155	4.010144E-8	9.814898	5
>>END_MODULE