FastQCFastQC Report
Thu 26 May 2016
SRR1513179_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1513179_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2015650
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28550.14164165405700394No Hit
GTACATGGGAAGCAGTGGTATCAAC23580.11698459553990029No Hit
CCCATGTACTCTGCGTTGATACCAC23020.11420633542529705No Hit
TATCAACGCAGAGTACTTTTTTTTT20540.1019026120606256No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCCG350.002187174516.2670659
ACCGTCC701.1026875E-412.2006038
GCGCCAC1052.7486567E-711.752810513
TAGGACC4600.011.5867554
TACACCG600.00579585211.1039735
CCCTATA1303.1721356E-810.9835912
GGCGAGG5350.010.81937319
TGACGAA1158.9506284E-710.72683310
CGTGCGC803.811859E-410.67526210
GTCTAAG1909.094947E-1210.525841
GACGAAA1451.465196E-810.47283411
TAGGACT2650.010.4155864
ACCGTGC1201.546141E-610.2801378
AGGACGT8850.010.2167315
AGGACTG3750.010.15220455
TGCACCG750.002607591510.1522045
TGGCGAG10400.010.13133118
ACACCGC856.487345E-410.0768036
CTAACAC1909.640644E-1110.0191213
TTAGGAC7900.010.0000973
Produced by FastQC (version 0.11.5)